Restriction map for
pFN21AE5936
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AhdI AlwI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BcnI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BseAI BseRI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SfcI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 592 GTAGAC GT^[AC][GT]AC
AflIII 572 ACATGT A^C[GA][CT]GT
AgeI 362 ACCGGT A^CCGGT
Alw44I 324 GTGCAC G^TGCAC
ApaLI 324 GTGCAC G^TGCAC
Asp700I 699 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 324 GTGCAC G[AT]GC[AT]^C
AvaI 779 CTCGAG C^[CT]CG[GA]G
AvaII 508 GGTCC G^G[AT]CC
BanII 415 GGGCTC G[GA]GC[CT]^C
BbvCI 495 CCTCAGC CCTCAGC(-5/-2)
BclI 128 TGATCA T^GATCA
BglI 346 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 545 CTCCAG CTGGAG(16/14)
BsaBI 129 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 208 GAGACC GGTCTC(1/5)
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BsiHKAI 324 GTGCAC G[AT]GC[AT]^C
BsmAI 208 GAGAC GTCTC(1/5)
BsmFI 509 GTCCC GGGAC(10/14)
BsmI 659 GCATTC GAATGC(1/-1)
BsoBI 779 CTCGAG C^[CT]CG[GA]G
BspLU11I 572 ACATGT A^CATGT
BsrDI 312 CATTGC GCAATG(2/0)
BsrFI 362 ACCGGT [GA]^CCGG[CT]
BssSI 20 CACGAG CACGAG(-5/-1)
BstEII 735 GGTCACC G^GT[ACGT]ACC
BstPI 735 GGTCACC G^GT[ACGT]ACC
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
EcoO65I 735 GGTCACC G^GT[ACGT]ACC
FauI 69 GCGGG CCCGC(4/6)
FbaI 128 TGATCA T^GATCA
HapII 363 CCGG C^CGG
HpaII 363 CCGG C^CGG
MaeII 125 ACGT A^CGT
MamI 129 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 554 TTAA T^TAA
MspI 363 CCGG C^CGG
PaeR7I 779 CTCGAG C^TCGAG
PflMI 723 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 362 ACCGGT A^CCGGT
PleI 23 GAGTC GAGTC(4/5)
PshAI 18 GACACGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 549 AGTACT AGT^ACT
SexAI 211 ACCTGGT A^CC[AT]GGT
SfiI 345 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 680 GCATGC GCATG^C
TfiI 47 GATTC G^A[AT]TC
Tru9I 554 TTAA T^TAA
Tsp509I 810 AATT ^AATT
Van91I 723 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 779 CTCGAG C^TCGAG
XmnI 699 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 113 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
498 CAGCTTCTG
BbvI 73 GCAGC GCAGC(8/12)
147 GCTGC
BglII 476 AGATCT A^GATCT
925 AGATCT
BsgI 163 CTGCAC GTGCAG(16/14)
564 CTGCAC
BstF5I 135 CATCC GGATG(2/0)
655 GGATG
BstYI 476 AGATCT [GA]^GATC[CT]
925 AGATCT
Cfr13I 446 GGGCC G^G[ACGT]CC
508 GGTCC
DsaI 350 CCATGG C^C[GA][CT]GG
411 CCATGG
EcoT14I 350 CCATGG C^C[AT][AT]GG
411 CCATGG
EcoT22I 657 ATGCAT ATGCA^T
678 ATGCAT
FokI 135 CATCC GGATG(9/13)
655 GGATG
HinfI 23 GAGTC G^A[ACGT]TC
47 GATTC
HphI 26 TCACC GGTGA(8/7)
737 TCACC
MflI 476 AGATCT [GA]^GATC[CT]
925 AGATCT
NcoI 350 CCATGG C^CATGG
411 CCATGG
NlaIV 439 GGTTCC GG[ACGT]^[ACGT]CC
508 GGTCCC
NsiI 657 ATGCAT ATGCA^T
678 ATGCAT
NspI 572 ACATGT [GA]CATG^[CT]
680 GCATGC
Sau96I 446 GGGCC G^G[ACGT]CC
508 GGTCC
SmlI 60 CTTGAG C^T[CT][GA]AG
779 CTCGAG
StyI 350 CCATGG C^C[AT][AT]GG
411 CCATGG
TaqI 774 TCGA T^CGA
780 TCGA
TseI 73 GCAGC G^C[AT]GC
147 GCTGC
TthHB8I 774 TCGA T^CGA
780 TCGA
XcmI 29 CCAAGTTCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
578 CCATAACTGAGTTGG
XhoII 476 AGATCT [GA]^GATC[CT]
925 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI BalI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cac8I DdeI DpnII
EaeI EcoRII Fnu4HI HaeIII ItaI
MaeIII MboI MboII MluNI MnlI
MscI MslI MvaI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI SfaNI
Tsp45I TspRI