################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BglI BglII BlnI BmyI BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeI MamI MluI MluNI MscI MspA1I MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 73 TCCGGA T^CCGGA AflII 459 CTTAAG C^TTAAG AvaI 480 CTCGAG C^[CT]CG[GA]G BclI 617 TGATCA T^GATCA BfrI 459 CTTAAG C^TTAAG BlpI 186 GCTGAGC GC^T[ACGT]AGC BpmI 226 CTGGAG CTGGAG(16/14) Bpu1102I 186 GCTGAGC GC^T[ACGT]AGC BsaWI 73 TCCGGA [AT]^CCGG[AT] BseAI 73 TCCGGA T^CCGGA BseRI 528 CTCCTC GAGGAG(10/8) BsmFI 704 GGGAC GGGAC(10/14) BsoBI 480 CTCGAG C^[CT]CG[GA]G BspEI 73 TCCGGA T^CCGGA BsrDI 686 GCAATG GCAATG(2/0) BsrFI 868 GCCGGC [GA]^CCGG[CT] BstYI 602 GGATCT [GA]^GATC[CT] Cac8I 868 GCCGGC GC[ACGT]^[ACGT]GC CelII 186 GCTGAGC GC^T[ACGT]AGC Cfr10I 868 GCCGGC [GA]^CCGG[CT] DraII 704 GGGACCC [GA]G^G[ACGT]CC[CT] DsaI 539 CCACGG C^C[GA][CT]GG EcoO109I 704 GGGACCC [GA]G^G[ACGT]CC[CT] FbaI 617 TGATCA T^GATCA HincII 574 GTCAAC GT[CT]^[GA]AC HindII 574 GTCAAC GT[CT]^[GA]AC HindIII 462 AAGCTT A^AGCTT HinfI 963 GACTC G^A[ACGT]TC MaeII 799 ACGT A^CGT MfeI 779 CAATTG C^AATTG MflI 602 GGATCT [GA]^GATC[CT] MroI 73 TCCGGA T^CCGGA MseI 460 TTAA T^TAA MslI 60 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 779 CAATTG C^AATTG NaeI 868 GCCGGC GCC^GGC NgoMIV 868 GCCGGC G^CCGGC PaeR7I 480 CTCGAG C^TCGAG PflMI 320 CCAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 963 GACTC GAGTC(4/5) PpuMI 704 GGGACCC [GA]G^G[AT]CC[CT] SapI 911 GAAGAGC GCTCTTC(1/4) ScaI 551 AGTACT AGT^ACT SexAI 578 ACCTGGT A^CC[AT]GGT SfcI 208 CTACAG C^T[GA][CT]AG SspI 832 AATATT AAT^ATT TaqI 481 TCGA T^CGA Tru9I 460 TTAA T^TAA TthHB8I 481 TCGA T^CGA Van91I 320 CCAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 480 CTCGAG C^TCGAG XhoII 602 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 171 CCGC CCGC(-3/-1) 924 GCGG AfaI 552 GTAC GT^AC 880 GTAC AlwI 648 GATCC GGATC(4/5) 602 GGATC AlwNI 822 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 961 CAGACTCTG Asp700I 231 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 549 GAAGTACTTC AvaII 698 GGTCC G^G[AT]CC 705 GGACC BbsI 349 GTCTTC GAAGAC(2/6) 490 GTCTTC BpuAI 349 GTCTTC GAAGAC(2/6) 490 GTCTTC BsaI 562 GAGACC GGTCTC(1/5) 69 GGTCTC BsrI 91 ACTGG ACTGG(1/-1) 163 ACTGG BstEII 51 GGTCACC G^GT[ACGT]ACC 166 GGTGACC BstPI 51 GGTCACC G^GT[ACGT]ACC 166 GGTGACC EaeI 119 CGGCCA [CT]^GGCC[GA] 870 CGGCCA EcoO65I 51 GGTCACC G^GT[ACGT]ACC 166 GGTGACC EcoT14I 99 CCTTGG C^C[AT][AT]GG 427 CCTTGG HapII 74 CCGG C^CGG 869 CCGG HpaII 74 CCGG C^CGG 869 CCGG MaeIII 52 GTCAC ^GT[ACGT]AC 167 GTGAC MspI 74 CCGG C^CGG 869 CCGG RsaI 552 GTAC GT^AC 880 GTAC StyI 99 CCTTGG C^C[AT][AT]GG 427 CCTTGG Tsp45I 52 GTCAC ^GT[GC]AC 167 GTGAC XmnI 231 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 549 GAAGTACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HphI ItaI Ksp632I MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI TseI Tsp509I TspRI