(Circular) (Six-base) MAPSORT of: pFN21AE5471.seq Check: 6878 from: 1 to: 5674 pFN21AE5471 5674 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3017 1937 Size: 1080 4594 Acc65I G'GTAC_C Cuts at: 2995 2995 Size: 5674 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2455 3737 5121 5389 1632 Size: 823 1282 1384 268 1917 Fragments arranged by size: 1917 1384 1282 823 268 AflII C'TTAA_G Cuts at: 820 1017 2464 820 Size: 197 1447 4030 Fragments arranged by size: 4030 1447 197 AflIII A'CryG_T Cuts at: 1203 4573 5550 1203 Size: 3370 977 1327 Fragments arranged by size: 3370 1327 977 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 2069 1166 Size: 903 4771 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5674 AlwNI CAG_nnn'CTG Cuts at: 2832 2971 3417 4989 2832 Size: 139 446 1572 3517 Fragments arranged by size: 3517 1572 446 139 ApaLI G'TGCA_C Cuts at: 3731 4887 3731 Size: 1156 4518 ApoI r'AATT_y Cuts at: 1493 2983 3209 3361 5470 1493 Size: 1490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 226 152 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2485 2999 1870 Size: 73 542 514 4545 Fragments arranged by size: 4545 542 514 73 BamHI G'GATC_C Cuts at: 3004 3371 3004 Size: 367 5307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2995 4450 618 Size: 325 217 627 1208 1455 1842 Fragments arranged by size: 1842 1455 1208 627 325 217 BanII G_rGCy'C Cuts at: 1391 2993 1391 Size: 1602 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2347 2488 928 Size: 971 448 141 4114 Fragments arranged by size: 4114 971 448 141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3865 3899 1903 Size: 34 1928 34 3678 Fragments arranged by size: 3678 1928 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 2622 1548 Size: 1074 4600 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4285 136 Size: 107 122 71 631 605 2613 1525 Fragments arranged by size: 2613 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5669 5669 Size: 5674 BlpI GC'TnA_GC Cuts at: 2192 3091 2192 Size: 899 4775 Bme1580I G_kGCm'C Cuts at: 1790 3735 4891 1790 Size: 1945 1156 2573 Fragments arranged by size: 2573 1945 1156 BmgBI CAC'GTC Cuts at: 1318 2805 1318 Size: 1487 4187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3405 4363 442 Size: 416 53 83 399 24 1988 958 1753 Fragments arranged by size: 1988 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 4334 1239 Size: 1013 2082 2579 Fragments arranged by size: 2579 2082 1013 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2648 3799 4664 4962 2648 Size: 1151 865 298 3360 Fragments arranged by size: 3360 1151 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2080 2561 4337 882 Size: 617 581 481 1776 2219 Fragments arranged by size: 2219 1776 617 581 481 BsaAI yAC'GTr Cuts at: 493 1204 3465 493 Size: 711 2261 2702 Fragments arranged by size: 2702 2261 711 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3481 3863 4498 275 Size: 53 83 186 936 1948 382 635 1451 Fragments arranged by size: 1948 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2078 4107 4505 4779 4926 5337 2078 Size: 2029 398 274 147 411 2415 Fragments arranged by size: 2415 2029 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2540 2570 1178 Size: 30 165 30 1137 30 4282 Fragments arranged by size: 4282 1137 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2524 1186 Size: 1338 4336 BseYI C'CCAG_C Cuts at: 2714 4877 5373 2714 Size: 2163 496 3015 Fragments arranged by size: 3015 2163 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 3885 4913 1540 Size: 275 186 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3735 3820 4891 3735 Size: 85 1071 4518 Fragments arranged by size: 4518 1071 85 BsmI GAATG_Cn' Cuts at: 3185 3278 3185 Size: 93 5581 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2993 3735 3820 4891 1391 Size: 399 1203 742 85 1071 2174 Fragments arranged by size: 2174 1203 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2078 5337 2078 Size: 3259 2415 BspHI T'CATG_A Cuts at: 3563 5293 3563 Size: 1730 3944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3029 844 Size: 2185 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3561 1394 Size: 429 1738 3507 Fragments arranged by size: 3507 1738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2698 4169 4343 65 Size: 1183 1450 1471 174 1396 Fragments arranged by size: 1471 1450 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2873 4318 4505 1700 Size: 110 1063 1445 187 2869 Fragments arranged by size: 2869 1445 1063 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5674 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5674 BssSI C'ACGA_G Cuts at: 3734 4746 3734 Size: 1012 4662 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5674 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 2056 2171 1184 Size: 872 115 4687 Fragments arranged by size: 4687 872 115 BstYI r'GATC_y Cuts at: 1972 2607 3004 3371 3756 3773 5225 5311 5323 Size: 635 397 367 385 17 1452 86 12 Cuts at: 5323 5669 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 635 397 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2544 513 Size: 552 939 540 3643 Fragments arranged by size: 3643 939 552 540 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4935 Fragments arranged by size: 4935 683 56 BtsI GCAGTG_nn' Cuts at: 3190 3984 4004 3190 Size: 794 20 4860 Fragments arranged by size: 4860 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2875 3121 Size: 54 1425 50 275 18 294 751 246 Cuts at: 3121 4010 5639 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 751 294 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2297 2402 2911 3604 1353 Size: 944 105 509 693 3423 Fragments arranged by size: 3423 944 693 509 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2455 3737 4334 5121 5389 1239 Size: 393 620 203 1282 597 787 268 1524 Fragments arranged by size: 1524 1282 787 620 597 393 268 203 EcoO109I rG'GnC_Cy Cuts at: 2710 2710 Size: 5674 EcoRI G'AATT_C Cuts at: 2983 3361 2983 Size: 378 5296 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 2575 2966 2077 Size: 498 391 4785 Fragments arranged by size: 4785 498 391 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 4821 1678 Size: 3143 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2644 2676 4315 4347 4389 Size: 32 135 32 1072 32 1639 32 42 Cuts at: 4389 4421 1373 Size: 32 2626 Fragments arranged by size: 2626 1639 1072 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2581 3018 3264 669 Size: 988 281 643 437 246 3079 Fragments arranged by size: 3079 988 643 437 281 246 HindIII A'AGCT_T Cuts at: 748 2467 748 Size: 1719 3955 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KpnI G_GTAC'C Cuts at: 2999 2999 Size: 5674 MfeI C'AATT_G Cuts at: 2784 3273 2784 Size: 489 5185 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2562 2832 4788 4972 1305 Size: 1257 270 1956 184 2007 Fragments arranged by size: 2007 1956 1257 270 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3852 Fragments arranged by size: 3852 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2069 3632 3991 4150 518 Size: 648 903 1563 359 159 2042 Fragments arranged by size: 2042 1563 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3090 3767 4915 5160 1606 Size: 1484 677 1148 245 2120 Fragments arranged by size: 2120 1484 1148 677 245 NaeI GCC'GGC Cuts at: 2875 2875 Size: 5674 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4183 Fragments arranged by size: 4183 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NgoMIV G'CCGG_C Cuts at: 2873 2873 Size: 5674 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 3032 4577 1132 Size: 97 1803 1545 2229 Fragments arranged by size: 2229 1803 1545 97 PasI CC'CwG_GG Cuts at: 1348 2906 1348 Size: 1558 4116 PciI A'CATG_T Cuts at: 4573 4573 Size: 5674 PflMI CCAn_nnn'nTGG Cuts at: 1108 2331 1108 Size: 1223 4451 PfoI T'CCnGG_A Cuts at: 2831 2831 Size: 5674 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2710 2710 Size: 5674 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 2268 3244 2268 Size: 976 4698 PstI C_TGCA'G Cuts at: 830 3026 830 Size: 2196 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SanDI GG'GwC_CC Cuts at: 2710 2710 Size: 5674 SapI GCTCTTCn'nnn_ Cuts at: 2911 2911 Size: 5674 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 2558 3922 5634 2558 Size: 1364 1712 2598 Fragments arranged by size: 2598 1712 1364 SexAI A'CCwGG_T Cuts at: 2583 2583 Size: 5674 SfcI C'TryA_G Cuts at: 826 1045 2213 3022 4157 4838 5029 826 Size: 219 1168 809 1135 681 191 1471 Fragments arranged by size: 1471 1168 1135 809 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 3001 1872 Size: 1129 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2464 2485 2663 3778 4679 4941 Size: 197 926 521 21 178 1115 901 262 Cuts at: 4941 820 Size: 1553 Fragments arranged by size: 1553 1115 926 901 521 262 197 178 21 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 2839 3598 5 Size: 47 2787 759 2081 Fragments arranged by size: 2787 2081 759 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 2004 2104 2432 3113 513 Size: 552 939 100 328 681 3074 Fragments arranged by size: 3074 939 681 552 328 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3701 3718 3871 4056 4501 325 Size: 1255 2121 17 153 185 445 1498 Fragments arranged by size: 2121 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2556 2884 3920 Size: 274 80 33 51 1443 579 328 1036 Cuts at: 3920 5632 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1036 579 328 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 2485 1943 Size: 542 5132 XmaI C'CCGG_G Cuts at: 1870 2999 1870 Size: 1129 4545 XmnI GAAnn'nnTTC Cuts at: 2240 2558 3803 2240 Size: 318 1245 4111 Fragments arranged by size: 4111 1245 318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI