################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspLU11I BsrBI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI KasI KpnI KspI MamI MluI MluNI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 875 TCCGGA T^CCGGA AcsI 28 GAATTT [GA]^AATT[CT] ApoI 28 GAATTT [GA]^AATT[CT] AseI 111 ATTAAT AT^TAAT AsnI 111 ATTAAT AT^TAAT Asp700I 705 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 360 GGACC G^G[AT]CC BfaI 17 CTAG C^TAG BpmI 202 CTGGAG CTGGAG(16/14) BsaAI 775 TACGTG [CT]AC^GT[GA] BsaWI 875 TCCGGA [AT]^CCGG[AT] BseAI 875 TCCGGA T^CCGGA BsmAI 646 GTCTC GTCTC(1/5) BspEI 875 TCCGGA T^CCGGA BspHI 134 TCATGA T^CATGA BspMI 418 ACCTGC ACCTGC(4/8) BsrDI 349 GCAATG GCAATG(2/0) BsrFI 474 GCCGGT [GA]^CCGG[CT] Cac8I 795 GCAGGC GC[ACGT]^[ACGT]GC Cfr10I 474 GCCGGT [GA]^CCGG[CT] DdeI 388 CTGAG C^T[ACGT]AG DraII 896 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 896 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 39 CCTTGG C^C[AT][AT]GG HincII 926 GTCAAC GT[CT]^[GA]AC HindII 926 GTCAAC GT[CT]^[GA]AC MaeI 17 CTAG C^TAG MroI 875 TCCGGA T^CCGGA PshBI 111 ATTAAT AT^TAAT PstI 160 CTGCAG CTGCA^G RcaI 134 TCATGA T^CATGA SapI 300 GCTCTTC GCTCTTC(1/4) SfcI 160 CTGCAG C^T[GA][CT]AG SmlI 676 CTCAAG C^T[CT][GA]AG StyI 39 CCTTGG C^C[AT][AT]GG TspRI 24 CACTG CA[GC]TG[ACGT][ACGT]^ XmnI 705 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 309 GTAC GT^AC 393 GTAC BstYI 206 AGATCC [GA]^GATC[CT] 680 AGATCC Cfr13I 360 GGACC G^G[ACGT]CC 897 GGCCC EarI 301 CTCTTC CTCTTC(1/4) 595 CTCTTC Ksp632I 301 CTCTTC CTCTTC(1/4) 595 CTCTTC MaeII 156 ACGT A^CGT 776 ACGT MflI 206 AGATCC [GA]^GATC[CT] 680 AGATCC MseI 112 TTAA T^TAA 601 TTAA MslI 129 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 399 CATCATTATG MspA1I 759 CAGCTG C[AC]G^C[GT]G 800 CAGCTG MwoI 44 GCTTCCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 752 GCTACGGCAGC PvuII 759 CAGCTG CAG^CTG 800 CAGCTG RsaI 309 GTAC GT^AC 393 GTAC Sau96I 360 GGACC G^G[ACGT]CC 897 GGCCC SfaNI 237 GATGC GCATC(5/9) 264 GATGC Tru9I 112 TTAA T^TAA 601 TTAA XhoII 206 AGATCC [GA]^GATC[CT] 680 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AlwI BbvI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboI MboII MfeI MnlI MspI MunI MvaI NdeII NlaIII Sau3AI ScrFI TseI Tsp45I Tsp509I