################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII AccI AclI AcsI AflII AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI AspI AviII AvrII BamHI BbeI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrBI BsrDI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DrdI Eco47III EcoRI EcoRV EcoT22I FbaI FspI HindIII HpaI KasI KspI MamI MfeI MluI MseI MunI NarI NdeI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tsp45I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 2256 TCCGGA T^CCGGA AcyI 781 GGCGTC G[GA]^CG[CT]C AhdI 1510 GACAAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1639 CCTGAGG CC^T[ACGT]AGG AspEI 1510 GACAAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1057 GAGCTC G[AT]GC[AT]^C BbsI 1200 GAAGAC GAAGAC(2/6) BbvCI 775 GCTGAGG CCTCAGC(-5/-2) BcgI 852 CGAAGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1200 GAAGAC GAAGAC(2/6) BsaHI 781 GGCGTC G[GA]^CG[CT]C BsaI 275 GAGACC GGTCTC(1/5) BsaWI 2256 TCCGGA [AT]^CCGG[AT] BseAI 2256 TCCGGA T^CCGGA BsiEI 153 CGGCCG CG[GA][CT]^CG BsiHKAI 1057 GAGCTC G[AT]GC[AT]^C Bsp1407I 1838 TGTACA T^GTACA BspEI 2256 TCCGGA T^CCGGA BsrGI 1838 TGTACA T^GTACA BssSI 1330 CACGAG CACGAG(-5/-1) BstEII 2178 GGTTACC G^GT[ACGT]ACC BstPI 2178 GGTTACC G^GT[ACGT]ACC Bsu36I 1639 CCTGAGG CC^T[ACGT]AGG EagI 153 CGGCCG C^GGCCG Eam1105I 1510 GACAAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1285 GAAGAG CTCTTC(1/4) EclXI 153 CGGCCG C^GGCCG Eco52I 153 CGGCCG C^GGCCG Eco81I 1639 CCTGAGG CC^T[ACGT]AGG EcoO65I 2178 GGTTACC G^GT[ACGT]ACC FseI 341 GGCCGGCC GGCCGG^CC Hin1I 781 GGCGTC G[GA]^CG[CT]C HincII 2095 GTCAAC GT[CT]^[GA]AC HindII 2095 GTCAAC GT[CT]^[GA]AC Ksp632I 1285 GAAGAG CTCTTC(1/4) MroI 2256 TCCGGA T^CCGGA NaeI 342 GCCGGC GCC^GGC NcoI 2186 CCATGG C^CATGG NgoMIV 342 GCCGGC G^CCGGC NotI 152 GCGGCCGC GC^GGCCGC SacI 1057 GAGCTC GAGCT^C SfaNI 1500 GATGC GCATC(5/9) SfiI 792 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 1838 TGTACA T^GTACA TaqI 2088 TCGA T^CGA TfiI 1440 GAATC G^A[AT]TC Tsp509I 1407 AATT ^AATT TthHB8I 2088 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 1153 GGTACC G^GTACC 2201 GGTACC AlwNI 185 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 556 CAGTTGCTG Asp700I 1380 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1593 GAAACGCTTC Asp718I 1153 GGTACC G^GTACC 2201 GGTACC BalI 902 TGGCCA TGG^CCA 1859 TGGCCA BbrPI 838 CACGTG CAC^GTG 1828 CACGTG BsaAI 838 CACGTG [CT]AC^GT[GA] 1828 CACGTG BsrI 1955 ACTGG ACTGG(1/-1) 501 CCAGT BstAPI 566 GCAGAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 810 GCAGGAGGTGC BstYI 959 AGATCC [GA]^GATC[CT] 1559 AGATCC DraIII 430 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG 1774 CACGCGGTG HgaI 158 GCGTC GACGC(5/10) 782 GCGTC KpnI 1153 GGTACC GGTAC^C 2201 GGTACC MaeII 839 ACGT A^CGT 1829 ACGT MaeIII 2179 GTTAC ^GT[ACGT]AC 2222 GTAAC MflI 959 AGATCC [GA]^GATC[CT] 1559 AGATCC MluNI 902 TGGCCA TGG^CCA 1859 TGGCCA MscI 902 TGGCCA TGG^CCA 1859 TGGCCA NheI 720 GCTAGC G^CTAGC 1696 GCTAGC NlaIII 2029 CATG CATG^ 2187 CATG PleI 756 GACTC GAGTC(4/5) 1220 GAGTC PmaCI 838 CACGTG CAC^GTG 1828 CACGTG PmlI 838 CACGTG CAC^GTG 1828 CACGTG SmlI 883 CTTGAG C^T[CT][GA]AG 1399 CTCAAG StuI 386 AGGCCT AGG^CCT 2022 AGGCCT XhoII 959 AGATCC [GA]^GATC[CT] 1559 AGATCC XmnI 1380 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1593 GAAACGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AfaI AluI AlwI ApaI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PflMI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfcI SmaI StyI TseI TspRI Van91I XcmI XmaCI XmaI