(Circular) (Six-base) MAPSORT of: pFN21AE3955.seq Check: 868 from: 1 to: 6970 pFN21AE3955 6970 bp With 182 enzymes: SgfI * March 28, 2012 11:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2893 2893 Size: 6970 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4780 278 Size: 53 83 186 936 3244 2468 Fragments arranged by size: 3244 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4313 1937 Size: 2376 4594 Acc65I G'GTAC_C Cuts at: 3158 4206 4291 3158 Size: 1048 85 5837 Fragments arranged by size: 5837 1048 85 AclI AA'CG_TT Cuts at: 1563 5097 5470 1563 Size: 3534 373 3063 Fragments arranged by size: 3534 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2643 5033 6417 6685 1632 Size: 1011 2390 1384 268 1917 Fragments arranged by size: 2390 1917 1384 1011 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6773 AflIII A'CryG_T Cuts at: 1203 5869 6846 1203 Size: 4666 977 1327 Fragments arranged by size: 4666 1327 977 AgeI A'CCGG_T Cuts at: 5801 5801 Size: 6970 AhdI GACnn_n'nnGTC Cuts at: 3520 5699 3520 Size: 2179 4791 AleI CACnn'nnGTG Cuts at: 1166 3363 4193 1166 Size: 2197 830 3943 Fragments arranged by size: 3943 2197 830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6970 AlwNI CAG_nnn'CTG Cuts at: 2195 2566 4713 6285 2195 Size: 371 2147 1572 2880 Fragments arranged by size: 2880 2147 1572 371 ApaI G_GGCC'C Cuts at: 2800 3325 3757 2800 Size: 525 432 6013 Fragments arranged by size: 6013 525 432 ApaLI G'TGCA_C Cuts at: 5027 6183 5027 Size: 1156 5814 ApoI r'AATT_y Cuts at: 1493 4279 4505 4657 6766 1493 Size: 2786 226 152 2109 1697 Fragments arranged by size: 2786 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5524 160 Size: 872 4492 1606 Fragments arranged by size: 4492 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 AvaI C'yCGr_G Cuts at: 1870 1943 2174 2204 2268 2487 2522 2751 2868 Size: 73 231 30 64 219 35 229 117 Cuts at: 2868 3285 3417 3558 4179 4295 1870 Size: 417 132 141 621 116 4545 Fragments arranged by size: 4545 621 417 231 229 219 141 132 117 116 73 64 35 30 BamHI G'GATC_C Cuts at: 4300 4667 4300 Size: 367 6603 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 2330 2361 2406 3025 Size: 325 217 627 305 238 31 45 619 Cuts at: 3025 3158 3629 4206 4291 5746 618 Size: 133 471 577 85 1455 1842 Fragments arranged by size: 1842 1455 627 619 577 471 325 305 238 217 133 85 45 31 BanII G_rGCy'C Cuts at: 1391 2800 2842 3066 3078 3253 3301 3325 3426 Size: 1409 42 224 12 175 48 24 101 Cuts at: 3426 3543 3757 4052 4289 1391 Size: 117 214 295 237 4072 Fragments arranged by size: 4072 1409 295 237 224 214 175 117 101 48 42 24 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3212 928 Size: 971 1313 4686 Fragments arranged by size: 4686 1313 971 BbvCI CC'TCA_GC Cuts at: 2781 2781 Size: 6970 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2846 2880 5161 5195 1903 Size: 34 909 34 2281 34 3678 Fragments arranged by size: 3678 2281 909 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4862 6072 4862 Size: 1210 5760 BclI T'GATC_A Cuts at: 1548 1548 Size: 6970 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2804 2915 4035 Size: 107 122 71 631 605 1132 111 1120 Cuts at: 4035 4047 5581 136 Size: 12 1534 1525 Fragments arranged by size: 1534 1525 1132 1120 631 605 122 111 107 71 12 BglII A'GATC_T Cuts at: 6965 6965 Size: 6970 BlpI GC'TnA_GC Cuts at: 4387 4387 Size: 6970 Bme1580I G_kGCm'C Cuts at: 1790 2095 2311 2409 2638 2800 3028 3325 3595 Size: 305 216 98 229 162 228 297 270 Cuts at: 3595 3632 3757 5031 6187 1790 Size: 37 125 1274 1156 2573 Fragments arranged by size: 2573 1274 1156 305 297 270 229 228 216 162 125 98 37 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6970 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2500 4701 5659 Size: 416 53 83 399 24 1083 2201 958 Cuts at: 5659 442 Size: 1753 Fragments arranged by size: 2201 1753 1083 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2729 3705 1056 Size: 1673 976 4321 Fragments arranged by size: 4321 1673 976 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2986 3670 2986 Size: 684 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2099 2672 2714 3080 3531 3903 5630 Size: 818 42 573 42 366 451 372 1727 Cuts at: 5630 1239 Size: 2579 Fragments arranged by size: 2579 1727 818 573 451 372 366 42 42 Bpu10I CC'TnA_GC Cuts at: 2045 2781 2999 3758 2045 Size: 736 218 759 5257 Fragments arranged by size: 5257 759 736 218 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2909 3389 5095 5960 6258 2909 Size: 480 1706 865 298 3621 Fragments arranged by size: 3621 1706 865 480 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2274 5633 882 Size: 617 775 3359 2219 Fragments arranged by size: 3359 2219 775 617 BsaAI yAC'GTr Cuts at: 493 1204 2845 3835 4761 493 Size: 711 1641 990 926 2702 Fragments arranged by size: 2702 1641 990 926 711 BsaBI GATnn'nnATC Cuts at: 6964 6964 Size: 6970 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2787 4777 5159 5794 Size: 53 83 186 936 1254 1990 382 635 Cuts at: 5794 275 Size: 1451 Fragments arranged by size: 1990 1451 1254 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4261 5403 5801 6075 6222 6633 4261 Size: 1142 398 274 147 411 4598 Fragments arranged by size: 4598 1142 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6745 Fragments arranged by size: 6745 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 2786 3131 3188 3326 3455 1186 Size: 1309 291 345 57 138 129 4701 Fragments arranged by size: 4701 1309 345 291 138 129 57 BseYI C'CCAG_C Cuts at: 2514 2824 3351 3425 3430 3994 6173 6669 2514 Size: 310 527 74 5 564 2179 496 2815 Fragments arranged by size: 2815 2179 564 527 496 310 74 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2398 2590 3761 1163 Size: 573 662 192 1171 4372 Fragments arranged by size: 4372 1171 662 573 192 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2161 4420 5181 6209 1540 Size: 275 186 160 2259 761 1028 2301 Fragments arranged by size: 2301 2259 1028 761 275 186 160 BsiHKAI G_wGCw'C Cuts at: 3066 5031 5116 6187 3066 Size: 1965 85 1071 3849 Fragments arranged by size: 3849 1965 1071 85 BsmI GAATG_Cn' Cuts at: 4481 4574 4481 Size: 93 6877 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 2311 2409 2638 2800 2842 3028 Size: 399 305 216 98 229 162 42 186 Cuts at: 3028 3066 3078 3253 3301 3325 3426 3543 3595 Size: 38 12 175 48 24 101 117 52 Cuts at: 3595 3632 3757 4052 4289 5031 5116 6187 1391 Size: 37 125 295 237 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 305 295 237 229 216 186 175 162 125 117 101 98 85 52 48 42 38 37 24 12 BspEI T'CCGG_A Cuts at: 4261 6633 4261 Size: 2372 4598 BspHI T'CATG_A Cuts at: 4859 6589 4859 Size: 1730 5240 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2292 2893 4194 4325 844 Size: 1448 601 1301 131 3489 Fragments arranged by size: 3489 1448 1301 601 131 BsrBI CCG'CTC Cuts at: 1394 1823 4857 1394 Size: 429 3034 3507 Fragments arranged by size: 3507 3034 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5465 5639 65 Size: 1183 4217 174 1396 Fragments arranged by size: 4217 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2347 2666 3637 5614 5801 1700 Size: 110 537 319 971 1977 187 2869 Fragments arranged by size: 2869 1977 971 537 319 187 110 BsrGI T'GTAC_A Cuts at: 96 3843 96 Size: 3747 3223 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6970 BssSI C'ACGA_G Cuts at: 3335 5030 6042 3335 Size: 1695 1012 4263 Fragments arranged by size: 4263 1695 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2577 2821 794 Size: 1783 244 4943 Fragments arranged by size: 4943 1783 244 BstBI TT'CG_AA Cuts at: 5774 5774 Size: 6970 BstEII G'GTnAC_C Cuts at: 1184 4183 1184 Size: 2999 3971 BstXI CCAn_nnnn'nTGG Cuts at: 3137 3341 3365 4007 4195 3137 Size: 204 24 642 188 5912 Fragments arranged by size: 5912 642 204 188 24 BstYI r'GATC_y Cuts at: 1972 2964 3564 4300 4667 5052 5069 6521 6607 Size: 992 600 736 367 385 17 1452 86 Cuts at: 6607 6619 6965 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 992 736 600 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3645 3645 Size: 6970 BtgI C'CryG_G Cuts at: 513 1065 2004 2014 2352 4162 4191 513 Size: 552 939 10 338 1810 29 3292 Fragments arranged by size: 3292 1810 939 552 338 29 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6231 Fragments arranged by size: 6231 683 56 BtsI GCAGTG_nn' Cuts at: 2102 2531 3749 3791 4486 5280 5300 2102 Size: 429 1218 42 695 794 20 3772 Fragments arranged by size: 3772 1218 794 695 429 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6970 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6935 DraI TTT'AAA Cuts at: 4274 4274 Size: 6970 DraIII CAC_nnn'GTG Cuts at: 2440 3784 2440 Size: 1344 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5977 809 Size: 507 4661 1802 Fragments arranged by size: 4661 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2158 2349 2664 Size: 54 1425 50 275 18 328 191 315 Cuts at: 2664 2907 3669 3864 4417 5306 6935 8 Size: 243 762 195 553 889 1629 43 Fragments arranged by size: 1629 1425 889 762 553 328 315 275 243 195 191 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2158 4417 1537 Size: 275 346 2259 4090 Fragments arranged by size: 4090 2259 346 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3285 4900 1353 Size: 1932 1615 3423 Fragments arranged by size: 3423 1932 1615 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2165 2897 3590 5557 5931 6077 Size: 1064 138 576 732 693 1967 374 146 Cuts at: 6077 387 Size: 1280 Fragments arranged by size: 1967 1280 1064 732 693 576 374 146 138 EcoICRI GAG'CTC Cuts at: 3064 3064 Size: 6970 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2099 2643 2672 2714 3080 3531 Size: 393 425 42 544 29 42 366 451 Cuts at: 3531 3903 5033 5630 6417 6685 1239 Size: 372 1130 597 787 268 1524 Fragments arranged by size: 1524 1130 787 597 544 451 425 393 372 366 268 42 42 29 EcoNI CCTnn'n_nnAGG Cuts at: 2036 2261 3767 2036 Size: 225 1506 5239 Fragments arranged by size: 5239 1506 225 EcoO109I rG'GnC_Cy Cuts at: 2019 2041 2797 3321 3347 3753 3754 3901 4131 Size: 22 756 524 26 406 1 147 230 Cuts at: 4131 2019 Size: 4858 Fragments arranged by size: 4858 756 524 406 230 147 26 22 1 EcoRI G'AATT_C Cuts at: 4279 4657 4279 Size: 378 6592 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2248 2248 Size: 6970 FseI GG_CCGG'CC Cuts at: 2351 2351 Size: 6970 FspI TGC'GCA Cuts at: 5476 5476 Size: 6970 HaeII r_GCGC'y Cuts at: 1678 2497 2726 3914 6117 1678 Size: 819 229 1188 2203 2531 Fragments arranged by size: 2531 2203 1188 819 229 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3506 3538 5611 5643 5685 Size: 32 135 32 1934 32 2073 32 42 Cuts at: 5685 5717 1373 Size: 32 2626 Fragments arranged by size: 2626 2073 1934 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4102 4314 4560 669 Size: 988 281 2164 212 246 3079 Fragments arranged by size: 3079 2164 988 281 246 212 HindIII A'AGCT_T Cuts at: 748 748 Size: 6970 HpaI GTT'AAC Cuts at: 4560 4560 Size: 6970 KpnI G_GTAC'C Cuts at: 3162 4210 4295 3162 Size: 1048 85 5837 Fragments arranged by size: 5837 1048 85 MfeI C'AATT_G Cuts at: 4569 4569 Size: 6970 MluI A'CGCG_T Cuts at: 6846 6846 Size: 6970 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3936 6084 6268 1305 Size: 2631 2148 184 2007 Fragments arranged by size: 2631 2148 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2909 3866 10 Size: 54 1425 343 1077 957 3114 Fragments arranged by size: 3114 1425 1077 957 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3339 3363 3503 4193 4928 5287 5446 Size: 648 2173 24 140 690 735 359 159 Cuts at: 5446 518 Size: 2042 Fragments arranged by size: 2173 2042 735 690 648 359 159 140 24 MspA1I CmG'CkG Cuts at: 1606 2086 2146 2169 2412 2995 3007 3058 4386 Size: 480 60 23 243 583 12 51 1328 Cuts at: 4386 5063 6211 6456 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1328 1148 677 583 480 245 243 60 51 23 12 NaeI GCC'GGC Cuts at: 2349 2349 Size: 6970 NcoI C'CATG_G Cuts at: 513 1065 2004 4191 513 Size: 552 939 2187 3292 Fragments arranged by size: 3292 2187 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6970 NgoMIV G'CCGG_C Cuts at: 2347 2347 Size: 6970 NheI G'CTAG_C Cuts at: 1052 2725 3701 1052 Size: 1673 976 4321 Fragments arranged by size: 4321 1673 976 NotI GC'GGCC_GC Cuts at: 2158 4417 2158 Size: 2259 4711 NspI r_CATG'y Cuts at: 1132 1229 4328 5873 1132 Size: 97 3099 1545 2229 Fragments arranged by size: 3099 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3021 3163 3436 3937 1348 Size: 1673 142 273 501 4381 Fragments arranged by size: 4381 1673 501 273 142 PciI A'CATG_T Cuts at: 5869 5869 Size: 6970 PflMI CCAn_nnn'nTGG Cuts at: 1108 2440 3307 3784 4168 1108 Size: 1332 867 477 384 3910 Fragments arranged by size: 3910 1332 867 477 384 PfoI T'CCnGG_A Cuts at: 2034 3078 3880 4116 2034 Size: 1044 802 236 4888 Fragments arranged by size: 4888 1044 802 236 PmeI GTTT'AAAC Cuts at: 4274 4274 Size: 6970 PmlI CAC'GTG Cuts at: 2845 3835 2845 Size: 990 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5039 5071 6552 6584 5039 Size: 32 1481 32 5425 Fragments arranged by size: 5425 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2019 3347 4131 2019 Size: 1328 784 4858 Fragments arranged by size: 4858 1328 784 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6970 PsiI TTA'TAA Cuts at: 4540 4540 Size: 6970 PspOMI G'GGCC_C Cuts at: 2796 3321 3753 2796 Size: 525 432 6013 Fragments arranged by size: 6013 525 432 PstI C_TGCA'G Cuts at: 830 3012 3491 3681 4322 830 Size: 2182 479 190 641 3478 Fragments arranged by size: 3478 2182 641 479 190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6970 PvuII CAG'CTG Cuts at: 2086 2146 3007 3058 2086 Size: 60 861 51 5998 Fragments arranged by size: 5998 861 60 51 SacI G_AGCT'C Cuts at: 3066 3066 Size: 6970 SalI G'TCGA_C Cuts at: 1936 4312 1936 Size: 2376 4594 SanDI GG'GwC_CC Cuts at: 4131 4131 Size: 6970 SbfI CC_TGCA'GG Cuts at: 4322 4322 Size: 6970 ScaI AGT'ACT Cuts at: 5218 6930 5218 Size: 1712 5258 SexAI A'CCwGG_T Cuts at: 2256 2448 2682 4209 2256 Size: 192 234 1527 5017 Fragments arranged by size: 5017 1527 234 192 SfcI C'TryA_G Cuts at: 826 1045 2458 3008 3487 3677 4318 5453 6134 Size: 219 1413 550 479 190 641 1135 681 Cuts at: 6134 6325 826 Size: 191 1471 Fragments arranged by size: 1471 1413 1135 681 641 550 479 219 191 190 SfiI GGCCn_nnn'nGGCC Cuts at: 2804 2804 Size: 6970 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6970 SmaI CCC'GGG Cuts at: 1872 2270 2489 2524 2753 3419 3560 4181 4297 Size: 398 219 35 229 666 141 621 116 Cuts at: 4297 1872 Size: 4545 Fragments arranged by size: 4545 666 621 398 229 219 141 116 35 SmlI C'TyrA_G Cuts at: 820 1017 1943 2888 3404 5074 5975 6237 820 Size: 197 926 945 516 1670 901 262 1553 Fragments arranged by size: 1670 1553 945 926 901 516 262 197 SnaBI TAC'GTA Cuts at: 493 4761 493 Size: 4268 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6970 SphI G_CATG'C Cuts at: 1132 4328 1132 Size: 3196 3774 SrfI GCCC'GGGC Cuts at: 2270 2270 Size: 6970 SspI AAT'ATT Cuts at: 5 52 4894 5 Size: 47 4842 2081 Fragments arranged by size: 4842 2081 47 StuI AGG'CCT Cuts at: 1517 2393 4029 1517 Size: 876 1636 4458 Fragments arranged by size: 4458 1636 876 StyI C'CwwG_G Cuts at: 513 1065 2004 3306 4138 4191 4409 513 Size: 552 939 1302 832 53 218 3074 Fragments arranged by size: 3074 1302 939 832 552 218 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2110 2315 2447 3542 4997 5014 5167 Size: 1255 530 205 132 1095 1455 17 153 Cuts at: 5167 5352 5797 325 Size: 185 445 1498 Fragments arranged by size: 1498 1455 1255 1095 530 445 205 185 153 132 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3843 4014 5216 Size: 274 80 33 51 1443 1866 171 1202 Cuts at: 5216 6928 96 Size: 1712 138 Fragments arranged by size: 1866 1712 1443 1202 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2523 2555 6930 6962 2523 Size: 32 4375 32 2531 Fragments arranged by size: 4375 2531 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6970 XbaI T'CTAG_A Cuts at: 4306 4306 Size: 6970 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3137 3242 3433 3781 3137 Size: 105 191 348 6326 Fragments arranged by size: 6326 348 191 105 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6970 XmaI C'CCGG_G Cuts at: 1870 2268 2487 2522 2751 3417 3558 4179 4295 Size: 398 219 35 229 666 141 621 116 Cuts at: 4295 1870 Size: 4545 Fragments arranged by size: 4545 666 621 398 229 219 141 116 35 XmnI GAAnn'nnTTC Cuts at: 3389 3602 5099 3389 Size: 213 1497 5260 Fragments arranged by size: 5260 1497 213 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4778 276 Size: 53 83 186 936 3244 2468 Fragments arranged by size: 3244 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SfoI SwaI