Restriction map for
pFN21AE3955
(Linear) (Six-base) MAPSORT of: KIEE3955.seq Check: 4488 from: 1 to: 2265
KIEE3955.orf
With 182 enzymes: SgfI *
March 22, 2012 16:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 888 2265
Size: 888 1377
Acc65I G'GTAC_C
Cuts at: 0 1153 2201 2265
Size: 1153 1048 64
Fragments arranged by size:
1153 1048 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 2265
Size: 638 1627
AhdI GACnn_n'nnGTC
Cuts at: 0 1515 2265
Size: 1515 750
AleI CACnn'nnGTG
Cuts at: 0 1358 2188 2265
Size: 1358 830 77
Fragments arranged by size:
1358 830 77
AlwNI CAG_nnn'CTG
Cuts at: 0 190 561 2265
Size: 190 371 1704
Fragments arranged by size:
1704 371 190
ApaI G_GGCC'C
Cuts at: 0 795 1320 1752 2265
Size: 795 525 432 513
Fragments arranged by size:
795 525 513 432
AvaI C'yCGr_G
Cuts at: 0 169 199 263 482 517 746 863 1280
Size: 169 30 64 219 35 229 117 417
Cuts at: 1280 1412 1553 2174 2265
Size: 132 141 621 91
Fragments arranged by size:
621 417 229 219 169 141 132 117
91 64 35 30
BanI G'GyrC_C
Cuts at: 0 87 325 356 401 1020 1153 1624 2201
Size: 87 238 31 45 619 133 471 577
Cuts at: 2201 2265
Size: 64
Fragments arranged by size:
619 577 471 238 133 87 64 45
31
BanII G_rGCy'C
Cuts at: 0 795 837 1061 1073 1248 1296 1320 1421
Size: 795 42 224 12 175 48 24 101
Cuts at: 1421 1538 1752 2047 2265
Size: 117 214 295 218
Fragments arranged by size:
795 295 224 218 214 175 117 101
48 42 24 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 1207 2265
Size: 1207 1058
BbvCI CC'TCA_GC
Cuts at: 0 776 2265
Size: 776 1489
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 841 875 2265
Size: 841 34 1390
Fragments arranged by size:
1390 841 34
BglI GCCn_nnn'nGGC
Cuts at: 0 799 910 2030 2042 2265
Size: 799 111 1120 12 223
Fragments arranged by size:
1120 799 223 111 12
Bme1580I G_kGCm'C
Cuts at: 0 90 306 404 633 795 1023 1320 1590
Size: 90 216 98 229 162 228 297 270
Cuts at: 1590 1627 1752 2265
Size: 37 125 513
Fragments arranged by size:
513 297 270 229 228 216 162 125
98 90 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 495 2265
Size: 495 1770
BmtI G_CTAG'C
Cuts at: 0 724 1700 2265
Size: 724 976 565
Fragments arranged by size:
976 724 565
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 981 1665 2265
Size: 981 684 600
Fragments arranged by size:
981 684 600
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 94 667 709 1075 1526 1898 2265
Size: 52 42 573 42 366 451 372 367
Fragments arranged by size:
573 451 372 367 366 52 42 42
Bpu10I CC'TnA_GC
Cuts at: 0 40 776 994 1753 2265
Size: 40 736 218 759 512
Fragments arranged by size:
759 736 512 218 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1384 2265
Size: 904 480 881
Fragments arranged by size:
904 881 480
BsaI GGTCTCn'nnnn_
Cuts at: 0 269 2265
Size: 269 1996
BsaAI yAC'GTr
Cuts at: 0 840 1830 2265
Size: 840 990 435
Fragments arranged by size:
990 840 435
BsaHI Gr'CG_yC
Cuts at: 0 782 2265
Size: 782 1483
BsaWI w'CCGG_w
Cuts at: 0 2256 2265
Size: 2256 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 781 1126 1183 1321 1450 2265
Size: 490 291 345 57 138 129 815
Fragments arranged by size:
815 490 345 291 138 129 57
BseYI C'CCAG_C
Cuts at: 0 509 819 1346 1420 1425 1989 2265
Size: 509 310 527 74 5 564 276
Fragments arranged by size:
564 527 509 310 276 74 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 585 1756 2265
Size: 393 192 1171 509
Fragments arranged by size:
1171 509 393 192
BsiEI CG_ry'CG
Cuts at: 0 156 2265
Size: 156 2109
BsiHKAI G_wGCw'C
Cuts at: 0 1061 2265
Size: 1061 1204
Bsp1286I G_dGCh'C
Cuts at: 0 90 306 404 633 795 837 1023 1061
Size: 90 216 98 229 162 42 186 38
Cuts at: 1061 1073 1248 1296 1320 1421 1538 1590 1627
Size: 12 175 48 24 101 117 52 37
Cuts at: 1627 1752 2047 2265
Size: 125 295 218
Fragments arranged by size:
295 229 218 216 186 175 162 125
117 101 98 90 52 48 42 38
37 24 12
BspEI T'CCGG_A
Cuts at: 0 2256 2265
Size: 2256 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 287 888 2189 2265
Size: 287 601 1301 76
Fragments arranged by size:
1301 601 287 76
BsrFI r'CCGG_y
Cuts at: 0 342 661 1632 2265
Size: 342 319 971 633
Fragments arranged by size:
971 633 342 319
BsrGI T'GTAC_A
Cuts at: 0 1838 2265
Size: 1838 427
BssSI C'ACGA_G
Cuts at: 0 1330 2265
Size: 1330 935
BstAPI GCAn_nnn'nTGC
Cuts at: 0 572 816 2265
Size: 572 244 1449
Fragments arranged by size:
1449 572 244
BstEII G'GTnAC_C
Cuts at: 0 2178 2265
Size: 2178 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1132 1336 1360 2002 2190 2265
Size: 1132 204 24 642 188 75
Fragments arranged by size:
1132 642 204 188 75 24
BstYI r'GATC_y
Cuts at: 0 959 1559 2265
Size: 959 600 706
Fragments arranged by size:
959 706 600
Bsu36I CC'TnA_GG
Cuts at: 0 1640 2265
Size: 1640 625
BtgI C'CryG_G
Cuts at: 0 9 347 2157 2186 2265
Size: 9 338 1810 29 79
Fragments arranged by size:
1810 338 79 29 9
BtsI GCAGTG_nn'
Cuts at: 0 97 526 1744 1786 2265
Size: 97 429 1218 42 479
Fragments arranged by size:
1218 479 429 97 42
DraIII CAC_nnn'GTG
Cuts at: 0 435 1779 2265
Size: 435 1344 486
Fragments arranged by size:
1344 486 435
EaeI y'GGCC_r
Cuts at: 0 153 344 659 902 1664 1859 2265
Size: 153 191 315 243 762 195 406
Fragments arranged by size:
762 406 315 243 195 191 153
EagI C'GGCC_G
Cuts at: 0 153 2265
Size: 153 2112
EarI CTCTTCn'nnn_
Cuts at: 0 1280 2265
Size: 1280 985
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 160 892 1585 2265
Size: 160 732 693 680
Fragments arranged by size:
732 693 680 160
EcoICRI GAG'CTC
Cuts at: 0 1059 2265
Size: 1059 1206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 94 638 667 709 1075 1526 1898
Size: 52 42 544 29 42 366 451 372
Cuts at: 1898 2265
Size: 367
Fragments arranged by size:
544 451 372 367 366 52 42 42
29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 256 1762 2265
Size: 31 225 1506 503
Fragments arranged by size:
1506 503 225 31
EcoO109I rG'GnC_Cy
Cuts at: 0 14 36 792 1316 1342 1748 1749 1896
Size: 14 22 756 524 26 406 1 147
Cuts at: 1896 2126 2265
Size: 230 139
Fragments arranged by size:
756 524 406 230 147 139 26 22
14 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 243 2265
Size: 243 2022
FseI GG_CCGG'CC
Cuts at: 0 346 2265
Size: 346 1919
HaeII r_GCGC'y
Cuts at: 0 492 721 1909 2265
Size: 492 229 1188 356
Fragments arranged by size:
1188 492 356 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1501 1533 2265
Size: 1501 32 732
Fragments arranged by size:
1501 732 32
HincII GTy'rAC
Cuts at: 0 2097 2265
Size: 2097 168
KpnI G_GTAC'C
Cuts at: 0 1157 2205 2265
Size: 1157 1048 60
Fragments arranged by size:
1157 1048 60
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1931 2265
Size: 1931 334
MscI TGG'CCA
Cuts at: 0 904 1861 2265
Size: 904 957 404
Fragments arranged by size:
957 904 404
MslI CAynn'nnrTG
Cuts at: 0 1334 1358 1498 2188 2265
Size: 1334 24 140 690 77
Fragments arranged by size:
1334 690 140 77 24
MspA1I CmG'CkG
Cuts at: 0 81 141 164 407 990 1002 1053 2265
Size: 81 60 23 243 583 12 51 1212
Fragments arranged by size:
1212 583 243 81 60 51 23 12
NaeI GCC'GGC
Cuts at: 0 344 2265
Size: 344 1921
NcoI C'CATG_G
Cuts at: 0 2186 2265
Size: 2186 79
NgoMIV G'CCGG_C
Cuts at: 0 342 2265
Size: 342 1923
NheI G'CTAG_C
Cuts at: 0 720 1696 2265
Size: 720 976 569
Fragments arranged by size:
976 720 569
NotI GC'GGCC_GC
Cuts at: 0 153 2265
Size: 153 2112
PasI CC'CwG_GG
Cuts at: 0 1016 1158 1431 1932 2265
Size: 1016 142 273 501 333
Fragments arranged by size:
1016 501 333 273 142
PflMI CCAn_nnn'nTGG
Cuts at: 0 435 1302 1779 2163 2265
Size: 435 867 477 384 102
Fragments arranged by size:
867 477 435 384 102
PfoI T'CCnGG_A
Cuts at: 0 29 1073 1875 2111 2265
Size: 29 1044 802 236 154
Fragments arranged by size:
1044 802 236 154 29
PmlI CAC'GTG
Cuts at: 0 840 1830 2265
Size: 840 990 435
Fragments arranged by size:
990 840 435
PpuMI rG'GwC_Cy
Cuts at: 0 14 1342 2126 2265
Size: 14 1328 784 139
Fragments arranged by size:
1328 784 139 14
PspOMI G'GGCC_C
Cuts at: 0 791 1316 1748 2265
Size: 791 525 432 517
Fragments arranged by size:
791 525 517 432
PstI C_TGCA'G
Cuts at: 0 1007 1486 1676 2265
Size: 1007 479 190 589
Fragments arranged by size:
1007 589 479 190
PvuII CAG'CTG
Cuts at: 0 81 141 1002 1053 2265
Size: 81 60 861 51 1212
Fragments arranged by size:
1212 861 81 60 51
SacI G_AGCT'C
Cuts at: 0 1061 2265
Size: 1061 1204
SanDI GG'GwC_CC
Cuts at: 0 2126 2265
Size: 2126 139
SexAI A'CCwGG_T
Cuts at: 0 251 443 677 2204 2265
Size: 251 192 234 1527 61
Fragments arranged by size:
1527 251 234 192 61
SfcI C'TryA_G
Cuts at: 0 453 1003 1482 1672 2265
Size: 453 550 479 190 593
Fragments arranged by size:
593 550 479 453 190
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 799 2265
Size: 799 1466
SmaI CCC'GGG
Cuts at: 0 265 484 519 748 1414 1555 2176 2265
Size: 265 219 35 229 666 141 621 89
Fragments arranged by size:
666 621 265 229 219 141 89 35
SmlI C'TyrA_G
Cuts at: 0 883 1399 2265
Size: 883 516 866
Fragments arranged by size:
883 866 516
SrfI GCCC'GGGC
Cuts at: 0 265 2265
Size: 265 2000
StuI AGG'CCT
Cuts at: 0 388 2024 2265
Size: 388 1636 241
Fragments arranged by size:
1636 388 241
StyI C'CwwG_G
Cuts at: 0 1301 2133 2186 2265
Size: 1301 832 53 79
Fragments arranged by size:
1301 832 79 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 105 310 442 1537 2265
Size: 105 205 132 1095 728
Fragments arranged by size:
1095 728 205 132 105
TatI w'GTAC_w
Cuts at: 0 1838 2009 2265
Size: 1838 171 256
Fragments arranged by size:
1838 256 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 518 550 2265
Size: 518 32 1715
Fragments arranged by size:
1715 518 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1132 1237 1428 1776 2265
Size: 1132 105 191 348 489
Fragments arranged by size:
1132 489 348 191 105
XmaI C'CCGG_G
Cuts at: 0 263 482 517 746 1412 1553 2174 2265
Size: 263 219 35 229 666 141 621 91
Fragments arranged by size:
666 621 263 229 219 141 91 35
XmnI GAAnn'nnTTC
Cuts at: 0 1384 1597 2265
Size: 1384 213 668
Fragments arranged by size:
1384 668 213
Enzymes that do cut:
AarI Acc65I AcuI AhdI AleI AlwNI ApaI AvaI
BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspMI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I
HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NheI NotI PasI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI SanDI SexAI SfcI SfiI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BglII BlpI BmgBI
BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrBI BsrDI
BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI
EcoRI EcoRV FspI FspAI HindIII HpaI KasI MfeI
MluI NarI NdeI NruI NsiI NspI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI
ZraI