################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HindIII HpaI KasI KpnI KspI MamI MflI MluI MluNI MroI MscI MslI MspA1I MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 652 GTAGAC GT^[AC][GT]AC AcyI 3 GGCGTC G[GA]^CG[CT]C Alw44I 779 GTGCAC G^TGCAC ApaLI 779 GTGCAC G^TGCAC AseI 144 ATTAAT AT^TAAT AsnI 144 ATTAAT AT^TAAT AvrII 826 CCTAGG C^CTAGG BbvI 544 GCTGC GCAGC(8/12) BclI 36 TGATCA T^GATCA BcnI 641 CCGGG CC^[GC]GG BlnI 826 CCTAGG C^CTAGG BsaAI 454 TACGTG [CT]AC^GT[GA] BsaHI 3 GGCGTC G[GA]^CG[CT]C Bsp1407I 1062 TGTACA T^GTACA BspHI 173 TCATGA T^CATGA BsrBI 9 GAGCGG CCGCTC(-3/-3) BsrGI 1062 TGTACA T^GTACA BsrI 907 ACTGG ACTGG(1/-1) BssSI 98 CTCGTG CACGAG(-5/-1) BstNI 775 CCTGG CC^[AT]GG Cac8I 769 GCCTGC GC[ACGT]^[ACGT]GC CfoI 849 GCGC GCG^C DraII 645 GGGACCT [GA]G^G[ACGT]CC[CT] EarI 860 GAAGAG CTCTTC(1/4) EcoO109I 645 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 775 CCTGG ^CC[AT]GG EcoT14I 826 CCTAGG C^C[AT][AT]GG FauI 14 GCGGG CCCGC(4/6) FbaI 36 TGATCA T^GATCA HapII 641 CCGG C^CGG HgaI 4 GCGTC GACGC(5/10) HhaI 849 GCGC GCG^C Hin1I 3 GGCGTC G[GA]^CG[CT]C HinP1I 849 GCGC G^CGC HincII 811 GTTGAC GT[CT]^[GA]AC HindII 811 GTTGAC GT[CT]^[GA]AC HpaII 641 CCGG C^CGG HphI 39 TCACC GGTGA(8/7) Ksp632I 860 GAAGAG CTCTTC(1/4) MfeI 212 CAATTG C^AATTG MspI 641 CCGG C^CGG MunI 212 CAATTG C^AATTG MvaI 775 CCTGG CC^[AT]GG NciI 641 CCGGG CC^[GC]GG NdeI 419 CATATG CA^TATG NlaIV 645 GGGACC GG[ACGT]^[ACGT]CC PpuMI 645 GGGACCT [GA]G^G[AT]CC[CT] PshBI 144 ATTAAT AT^TAAT RcaI 173 TCATGA T^CATGA ScaI 137 AGTACT AGT^ACT SspBI 1062 TGTACA T^GTACA StyI 826 CCTAGG C^C[AT][AT]GG TseI 544 GCTGC G^C[AT]GC Tsp45I 457 GTGAC ^GT[GC]AC TspRI 764 CAGTG CA[GC]TG[ACGT][ACGT]^ XbaI 231 TCTAGA T^CTAGA XcmI 308 CCAAGAGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AluI 20 AGCT AG^CT 543 AGCT AspHI 659 GAGCAC G[AT]GC[AT]^C 779 GTGCAC AvaII 646 GGACC G^G[AT]CC 928 GGACC BbsI 250 GAAGAC GAAGAC(2/6) 798 GTCTTC BmyI 659 GAGCAC G[AGT]GC[ACT]^C 779 GTGCAC BpmI 365 CTCCAG CTGGAG(16/14) 1040 CTCCAG BpuAI 250 GAAGAC GAAGAC(2/6) 798 GTCTTC BseRI 618 CTCCTC GAGGAG(10/8) 669 CTCCTC BsiHKAI 659 GAGCAC G[AT]GC[AT]^C 779 GTGCAC BsiYI 48 CCAGAGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 160 CCTGCTGTGGG BslI 48 CCAGAGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 160 CCTGCTGTGGG BsmFI 87 GGGAC GGGAC(10/14) 645 GGGAC Bsp1286I 659 GAGCAC G[AGT]GC[ACT]^C 779 GTGCAC BssKI 641 CCGGG ^CC[ACGT]GG 775 CCTGG DpnII 37 GATC ^GATC 871 GATC Fnu4HI 11 GCGGC GC^[ACGT]GC 544 GCTGC ItaI 11 GCGGC GC^[ACGT]GC 544 GCTGC MboI 37 GATC ^GATC 871 GATC MwoI 298 GCAAGGACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 781 GCACAAAAGGC NdeII 37 GATC ^GATC 871 GATC NlaIII 174 CATG CATG^ 352 CATG PstI 560 CTGCAG CTGCA^G 841 CTGCAG Sau3AI 37 GATC ^GATC 871 GATC ScrFI 641 CCGGG CC^[ACGT]GG 775 CCTGG TaqI 7 TCGA T^CGA 81 TCGA TfiI 130 GAATC G^A[AT]TC 194 GATTC TthHB8I 7 TCGA T^CGA 81 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AcsI AfaI ApoI BfaI BsaJI BstF5I Cfr13I DdeI FokI HaeIII HinfI MaeI MaeII MaeIII MboII MnlI MseI PleI RsaI Sau96I SfcI Tru9I Tsp509I