(Circular) (Six-base) MAPSORT of: pFN21AE2191.seq Check: 5770 from: 1 to: 5806 pFN21AE2191 5806 bp With 182 enzymes: SgfI * April 19, 2016 16:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2658 3149 1937 Size: 721 491 4594 Fragments arranged by size: 4594 721 491 Acc65I G'GTAC_C Cuts at: 3127 3127 Size: 5806 AclI AA'CG_TT Cuts at: 1563 3933 4306 1563 Size: 2370 373 3063 Fragments arranged by size: 3063 2370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 2790 3869 5253 5521 1632 Size: 654 504 1079 1384 268 1917 Fragments arranged by size: 1917 1384 1079 654 504 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 4705 5682 1203 Size: 3502 977 1327 Fragments arranged by size: 3502 1327 977 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 4535 4535 Size: 5806 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5806 AlwNI CAG_nnn'CTG Cuts at: 3549 5121 3549 Size: 1572 4234 ApaLI G'TGCA_C Cuts at: 2784 3863 5019 2784 Size: 1079 1156 3571 Fragments arranged by size: 3571 1156 1079 ApoI r'AATT_y Cuts at: 1493 2282 2375 2995 3115 3341 3493 5602 1493 Size: 789 93 620 120 226 152 2109 1697 Fragments arranged by size: 2109 1697 789 620 226 152 120 93 AseI AT'TA_AT Cuts at: 160 1032 2150 4360 160 Size: 872 1118 2210 1606 Fragments arranged by size: 2210 1606 1118 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 3131 1870 Size: 73 1188 4545 Fragments arranged by size: 4545 1188 73 AvrII C'CTAG_G Cuts at: 2831 2831 Size: 5806 BamHI G'GATC_C Cuts at: 3136 3503 3136 Size: 367 5439 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3127 4582 618 Size: 325 217 627 1340 1455 1842 Fragments arranged by size: 1842 1455 1340 627 325 217 BanII G_rGCy'C Cuts at: 1391 3125 1391 Size: 1734 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2262 2796 928 Size: 971 363 534 3938 Fragments arranged by size: 3938 971 534 363 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3997 4031 1903 Size: 34 2060 34 3678 Fragments arranged by size: 3678 2060 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3698 4908 3698 Size: 1210 4596 BclI T'GATC_A Cuts at: 1548 2041 1548 Size: 493 5313 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4417 136 Size: 107 122 71 631 605 2745 1525 Fragments arranged by size: 2745 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5801 5801 Size: 5806 BlpI GC'TnA_GC Cuts at: 3223 3223 Size: 5806 Bme1580I G_kGCm'C Cuts at: 1790 2788 3867 5023 1790 Size: 998 1079 1156 2573 Fragments arranged by size: 2573 1156 1079 998 BmgBI CAC'GTC Cuts at: 1318 2050 1318 Size: 732 5074 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3537 4495 442 Size: 416 53 83 399 24 2120 958 1753 Fragments arranged by size: 2120 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2355 3030 4466 1239 Size: 1116 675 1436 2579 Fragments arranged by size: 2579 1436 1116 675 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3931 4796 5094 3931 Size: 865 298 4643 Fragments arranged by size: 4643 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4469 882 Size: 617 2970 2219 Fragments arranged by size: 2970 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2461 3597 493 Size: 711 1257 1136 2702 Fragments arranged by size: 2702 1257 1136 711 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3613 3995 4630 Size: 53 83 186 936 476 1604 382 635 Cuts at: 4630 275 Size: 1451 Fragments arranged by size: 1604 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4239 4637 4911 5058 5469 4239 Size: 398 274 147 411 4576 Fragments arranged by size: 4576 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5581 Fragments arranged by size: 5581 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2614 2665 1186 Size: 1428 51 4327 Fragments arranged by size: 4327 1428 51 BseYI C'CCAG_C Cuts at: 2248 5009 5505 2248 Size: 2761 496 2549 Fragments arranged by size: 2761 2549 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5233 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3256 4017 5045 1540 Size: 275 186 1255 761 1028 2301 Fragments arranged by size: 2301 1255 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2668 2788 3867 3952 5023 2668 Size: 120 1079 85 1071 3451 Fragments arranged by size: 3451 1079 1071 120 85 BsmI GAATG_Cn' Cuts at: 3317 3410 3317 Size: 93 5713 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2668 2788 3125 3867 3952 5023 1391 Size: 399 878 120 337 742 85 1071 2174 Fragments arranged by size: 2174 1071 878 742 399 337 120 85 BspEI T'CCGG_A Cuts at: 5469 5469 Size: 5806 BspHI T'CATG_A Cuts at: 2178 3695 5425 2178 Size: 1517 1730 2559 Fragments arranged by size: 2559 1730 1517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3161 844 Size: 2317 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2016 3693 1394 Size: 429 193 1677 3507 Fragments arranged by size: 3507 1677 429 193 BsrDI GCAATG_nn' Cuts at: 65 1248 4301 4475 65 Size: 1183 3053 174 1396 Fragments arranged by size: 3053 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4450 4637 1700 Size: 110 2640 187 2869 Fragments arranged by size: 2869 2640 187 110 BsrGI T'GTAC_A Cuts at: 96 3067 96 Size: 2971 2835 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5806 BssSI C'ACGA_G Cuts at: 2103 3866 4878 2103 Size: 1763 1012 3031 Fragments arranged by size: 3031 1763 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5806 BstBI TT'CG_AA Cuts at: 4610 4610 Size: 5806 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5806 BstYI r'GATC_y Cuts at: 1972 3136 3503 3888 3905 5357 5443 5455 5801 Size: 1164 367 385 17 1452 86 12 346 Cuts at: 5801 1972 Size: 1977 Fragments arranged by size: 1977 1452 1164 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4315 Fragments arranged by size: 4315 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5067 Fragments arranged by size: 5067 683 56 BtsI GCAGTG_nn' Cuts at: 3322 4116 4136 3322 Size: 794 20 4992 Fragments arranged by size: 4992 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3253 4142 5771 Size: 54 1425 50 275 18 1423 889 1629 Cuts at: 5771 8 Size: 43 Fragments arranged by size: 1629 1425 1423 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3253 1537 Size: 275 1441 4090 Fragments arranged by size: 4090 1441 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2860 3736 1353 Size: 1507 876 3423 Fragments arranged by size: 3423 1507 876 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4393 4767 4913 387 Size: 1064 138 2804 374 146 1280 Fragments arranged by size: 2804 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2355 2790 3030 3869 4466 5253 Size: 393 654 69 435 240 839 597 787 Cuts at: 5253 5521 1239 Size: 268 1524 Fragments arranged by size: 1524 839 787 654 597 435 393 268 240 69 EcoO109I rG'GnC_Cy Cuts at: 2651 2651 Size: 5806 EcoRI G'AATT_C Cuts at: 3115 3493 3115 Size: 378 5428 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2291 2291 Size: 5806 FspI TGC'GCA Cuts at: 4312 4312 Size: 5806 HaeII r_GCGC'y Cuts at: 1678 4953 1678 Size: 3275 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2507 2539 2920 2952 4447 Size: 32 135 32 935 32 381 32 1495 Cuts at: 4447 4479 4521 4553 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1495 935 381 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2818 3150 3396 669 Size: 988 281 880 332 246 3079 Fragments arranged by size: 3079 988 880 332 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5806 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KpnI G_GTAC'C Cuts at: 3131 3131 Size: 5806 MfeI C'AATT_G Cuts at: 2217 3405 2217 Size: 1188 4618 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4920 5104 1305 Size: 3615 184 2007 Fragments arranged by size: 3615 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3984 Fragments arranged by size: 3984 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3764 4123 4282 518 Size: 648 2598 359 159 2042 Fragments arranged by size: 2598 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3222 3899 5047 5292 1606 Size: 1616 677 1148 245 2120 Fragments arranged by size: 2120 1616 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4315 Fragments arranged by size: 4315 939 552 NdeI CA'TA_TG Cuts at: 387 2425 387 Size: 2038 3768 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 3253 3253 Size: 5806 NspI r_CATG'y Cuts at: 1132 1229 3164 4709 1132 Size: 97 1935 1545 2229 Fragments arranged by size: 2229 1935 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 4705 4705 Size: 5806 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5806 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2651 2651 Size: 5806 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 2718 3376 2718 Size: 658 5148 PstI C_TGCA'G Cuts at: 830 2569 2850 3158 830 Size: 1739 281 308 3478 Fragments arranged by size: 3478 1739 308 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 2144 4054 5766 2144 Size: 1910 1712 2184 Fragments arranged by size: 2184 1910 1712 SfcI C'TryA_G Cuts at: 826 1045 2565 2655 2846 3154 4289 4970 5161 Size: 219 1520 90 191 308 1135 681 191 Cuts at: 5161 826 Size: 1471 Fragments arranged by size: 1520 1471 1135 681 308 219 191 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5806 SmaI CCC'GGG Cuts at: 1872 3133 1872 Size: 1261 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3910 4811 5073 820 Size: 197 926 1967 901 262 1553 Fragments arranged by size: 1967 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3597 493 Size: 3104 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 3164 1132 Size: 2032 3774 SspI AAT'ATT Cuts at: 5 52 3730 5 Size: 47 3678 2081 Fragments arranged by size: 3678 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5806 StyI C'CwwG_G Cuts at: 513 1065 2004 2831 3245 513 Size: 552 939 827 414 3074 Fragments arranged by size: 3074 939 827 552 414 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3833 3850 4003 4188 4633 325 Size: 1255 2253 17 153 185 445 1498 Fragments arranged by size: 2253 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 2226 3067 Size: 274 80 33 51 1443 165 84 841 Cuts at: 3067 4052 5764 96 Size: 985 1712 138 Fragments arranged by size: 1712 1443 985 841 274 165 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5766 5798 5766 Size: 32 5774 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 2236 3142 2236 Size: 906 4900 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 2320 Size: 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 3131 1870 Size: 1261 4545 XmnI GAAnn'nnTTC Cuts at: 3935 3935 Size: 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI