(Linear) (Six-base) MAPSORT of: KIEE2191.seq  Check: 7139  from: 1  to: 1101



KIEE2191.orf

 With 182 enzymes: SgfI * 

                        April 19, 2016 16:23  ..

AccI GT'mk_AC

Cuts at:      0     653    1101 
   Size:        653     448
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     785    1101 
   Size:        281     504     316

  Fragments arranged by size: 

                 504     316     281
 
ApaLI G'TGCA_C

Cuts at:      0     779    1101 
   Size:        779     322
 
ApoI r'AATT_y

Cuts at:      0     277     370     990    1101 
   Size:        277      93     620     111

  Fragments arranged by size: 

                 620     277     111      93
 
AseI AT'TA_AT

Cuts at:      0     145    1101 
   Size:        145     956
 
AvrII C'CTAG_G

Cuts at:      0     826    1101 
   Size:        826     275
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     257     791    1101 
   Size:        257     534     310

  Fragments arranged by size: 

                 534     310     257
 
BclI T'GATC_A

Cuts at:      0      36    1101 
   Size:         36    1065
 
Bme1580I G_kGCm'C

Cuts at:      0     783    1101 
   Size:        783     318
 
BmgBI CAC'GTC

Cuts at:      0      45    1101 
   Size:         45    1056
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350    1025    1101 
   Size:        350     675      76

  Fragments arranged by size: 

                 675     350      76
 
BsaAI yAC'GTr

Cuts at:      0     456    1101 
   Size:        456     645
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1101 
   Size:          4    1097
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     609     660    1101 
   Size:        609      51     441

  Fragments arranged by size: 

                 609     441      51
 
BseYI C'CCAG_C

Cuts at:      0     243    1101 
   Size:        243     858
 
BsiHKAI G_wGCw'C

Cuts at:      0     663     783    1101 
   Size:        663     120     318

  Fragments arranged by size: 

                 663     318     120
 
Bsp1286I G_dGCh'C

Cuts at:      0     663     783    1101 
   Size:        663     120     318

  Fragments arranged by size: 

                 663     318     120
 
BspHI T'CATG_A

Cuts at:      0     173    1101 
   Size:        173     928
 
BsrBI CCG'CTC

Cuts at:      0      11    1101 
   Size:         11    1090
 
BsrGI T'GTAC_A

Cuts at:      0    1062    1101 
   Size:       1062      39
 
BssSI C'ACGA_G

Cuts at:      0      98    1101 
   Size:         98    1003
 
EarI CTCTTCn'nnn_

Cuts at:      0     855    1101 
   Size:        855     246
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     350     785    1025    1101 
   Size:        281      69     435     240      76

  Fragments arranged by size: 

                 435     281     240      76      69
 
EcoO109I rG'GnC_Cy

Cuts at:      0     646    1101 
   Size:        646     455
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     286    1101 
   Size:        286     815
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     502     534     915     947    1101 
   Size:        502      32     381      32     154

  Fragments arranged by size: 

                 502     381     154      32      32
 
HincII GTy'rAC

Cuts at:      0     813    1101 
   Size:        813     288
 
MfeI C'AATT_G

Cuts at:      0     212    1101 
   Size:        212     889
 
NdeI CA'TA_TG

Cuts at:      0     420    1101 
   Size:        420     681
 
PpuMI rG'GwC_Cy

Cuts at:      0     646    1101 
   Size:        646     455
 
PsiI TTA'TAA

Cuts at:      0     713    1101 
   Size:        713     388
 
PstI C_TGCA'G

Cuts at:      0     564     845    1101 
   Size:        564     281     256

  Fragments arranged by size: 

                 564     281     256
 
ScaI AGT'ACT

Cuts at:      0     139    1101 
   Size:        139     962
 
SfcI C'TryA_G

Cuts at:      0     560     650     841    1101 
   Size:        560      90     191     260

  Fragments arranged by size: 

                 560     260     191      90
 
StyI C'CwwG_G

Cuts at:      0     826    1101 
   Size:        826     275
 
TatI w'GTAC_w

Cuts at:      0     137     221    1062    1101 
   Size:        137      84     841      39

  Fragments arranged by size: 

                 841     137      84      39
 
XbaI T'CTAG_A

Cuts at:      0     231    1101 
   Size:        231     870
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     315    1101 
   Size:        315     786
 

 Enzymes that do cut:

     AccI     AcuI    ApaLI     ApoI     AseI    AvrII     BbsI     BclI
 Bme1580I    BmgBI     BpmI    BsaAI    BsaHI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspHI    BsrBI    BsrGI    BssSI     EarI  Eco57MI EcoO109I
     FalI    Hin4I   HincII     MfeI     NdeI    PpuMI     PsiI     PstI
     ScaI     SfcI     StyI     TatI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrDI    BsrFI   BssHII
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XhoI     XmaI     XmnI     ZraI