################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BbeI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 1347 CCGC CCGC(-3/-1) AcsI 39 GAATTC [GA]^AATT[CT] AflIII 1259 ACGTGT A^C[GA][CT]GT ApoI 39 GAATTC [GA]^AATT[CT] AspHI 434 GAGCAC G[AT]GC[AT]^C BamHI 1343 GGATCC G^GATCC BanI 372 GGTGCC G^G[CT][GA]CC BbrPI 385 CACGTG CAC^GTG BclI 1295 TGATCA T^GATCA BseRI 1017 CTCCTC GAGGAG(10/8) BsgI 182 GTGCAG GTGCAG(16/14) BsiHKAI 434 GAGCAC G[AT]GC[AT]^C BsmBI 662 GAGACG CGTCTC(1/5) Bsp1407I 1180 TGTACA T^GTACA BspMI 453 GCAGGT ACCTGC(4/8) BsrDI 1112 GCAATG GCAATG(2/0) BsrGI 1180 TGTACA T^GTACA BstAPI 436 GCACTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1343 GGATCC [GA]^GATC[CT] Cac8I 762 GCTTGC GC[ACGT]^[ACGT]GC EarI 1209 GAAGAG CTCTTC(1/4) EcoRI 39 GAATTC G^AATTC FbaI 1295 TGATCA T^GATCA HincII 1120 GTCAAC GT[CT]^[GA]AC HindII 1120 GTCAAC GT[CT]^[GA]AC Ksp632I 1209 GAAGAG CTCTTC(1/4) MaeIII 976 GTTAC ^GT[ACGT]AC MflI 1343 GGATCC [GA]^GATC[CT] MwoI 436 GCACTGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 1040 CATATG CA^TATG PflMI 1188 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 385 CACGTG CAC^GTG PmlI 385 CACGTG CAC^GTG SspBI 1180 TGTACA T^GTACA SspI 297 AATATT AAT^ATT Van91I 1188 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1343 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccI 304 GTAGAC GT^[AC][GT]AC 1324 GTAGAC Asp700I 1088 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1209 GAAGAGATTC BanII 380 GAGCCC G[GA]GC[CT]^C 419 GGGCTC BbsI 159 GAAGAC GAAGAC(2/6) 634 GTCTTC BbvCI 1019 CCTCAGC CCTCAGC(-5/-2) 1218 CCTCAGC BpuAI 159 GAAGAC GAAGAC(2/6) 634 GTCTTC BsaAI 385 CACGTG [CT]AC^GT[GA] 1258 TACGTG BsmAI 58 GAGAC GTCTC(1/5) 662 GAGAC BstF5I 441 GGATG GGATG(2/0) 857 GGATG FokI 441 GGATG GGATG(9/13) 857 GGATG HphI 82 GGTGA GGTGA(8/7) 1293 GGTGA MslI 921 CACACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1191 CACCCTGGTG PstI 580 CTGCAG CTGCA^G 1250 CTGCAG SfcI 580 CTGCAG C^T[GA][CT]AG 1250 CTGCAG SmlI 192 CTTGAG C^T[CT][GA]AG 991 CTCAAG XmnI 1088 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1209 GAAGAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AfaI AluI AlwI BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI DdeI DpnII EcoRII Fnu4HI HinfI ItaI MaeII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I