(Circular) (Six-base) MAPSORT of: pFN21AE2415.seq Check: 5406 from: 1 to: 6082 pFN21AE2415 6082 bp With 182 enzymes: SgfI * July 10, 2012 14:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2449 2449 Size: 6082 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3892 278 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2310 3330 3425 1937 Size: 373 1020 95 4594 Fragments arranged by size: 4594 1020 373 95 Acc65I G'GTAC_C Cuts at: 3403 3403 Size: 6082 AclI AA'CG_TT Cuts at: 1563 4209 4582 1563 Size: 2646 373 3063 Fragments arranged by size: 3063 2646 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 4145 5529 5797 1632 Size: 402 2111 1384 268 1917 Fragments arranged by size: 2111 1917 1384 402 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5885 AflIII A'CryG_T Cuts at: 1203 3264 3379 4981 5958 1203 Size: 2061 115 1602 977 1327 Fragments arranged by size: 2061 1602 1327 977 115 AgeI A'CCGG_T Cuts at: 4913 4913 Size: 6082 AhdI GACnn_n'nnGTC Cuts at: 4811 4811 Size: 6082 AleI CACnn'nnGTG Cuts at: 1166 2930 3200 1166 Size: 1764 270 4048 Fragments arranged by size: 4048 1764 270 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6082 AlwNI CAG_nnn'CTG Cuts at: 3825 5397 3825 Size: 1572 4510 ApaLI G'TGCA_C Cuts at: 4139 5295 4139 Size: 1156 4926 ApoI r'AATT_y Cuts at: 1493 2044 3391 3617 3769 5878 1493 Size: 551 1347 226 152 2109 1697 Fragments arranged by size: 2109 1697 1347 551 226 152 AseI AT'TA_AT Cuts at: 160 1032 4636 160 Size: 872 3604 1606 Fragments arranged by size: 3604 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 AvaI C'yCGr_G Cuts at: 1870 1943 3407 1870 Size: 73 1464 4545 Fragments arranged by size: 4545 1464 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2798 2831 2798 Size: 33 6049 BamHI G'GATC_C Cuts at: 3348 3412 3779 3348 Size: 64 367 5651 Fragments arranged by size: 5651 367 64 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2377 3403 4858 618 Size: 325 217 627 590 1026 1455 1842 Fragments arranged by size: 1842 1455 1026 627 590 325 217 BanII G_rGCy'C Cuts at: 1391 2389 2428 3401 1391 Size: 998 39 973 4072 Fragments arranged by size: 4072 998 973 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2171 2632 928 Size: 971 272 461 4378 Fragments arranged by size: 4378 971 461 272 BbvCI CC'TCA_GC Cuts at: 3025 3224 3025 Size: 199 5883 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4273 4307 1903 Size: 34 2336 34 3678 Fragments arranged by size: 3678 2336 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3974 5184 3974 Size: 1210 4872 BclI T'GATC_A Cuts at: 1548 3300 1548 Size: 1752 4330 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4693 136 Size: 107 122 71 631 605 3021 1525 Fragments arranged by size: 3021 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6077 6077 Size: 6082 BlpI GC'TnA_GC Cuts at: 3499 3499 Size: 6082 Bme1580I G_kGCm'C Cuts at: 1790 4143 5299 1790 Size: 2353 1156 2573 Fragments arranged by size: 2573 2353 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6082 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2114 2175 3170 Size: 416 53 83 399 24 697 61 995 Cuts at: 3170 3813 4771 442 Size: 643 958 1753 Fragments arranged by size: 1753 995 958 697 643 416 399 83 61 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6082 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2414 3151 4742 1239 Size: 866 309 737 1591 2579 Fragments arranged by size: 2579 1591 866 737 309 Bpu10I CC'TnA_GC Cuts at: 3025 3224 3025 Size: 199 5883 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2218 2981 4207 5072 5370 2218 Size: 763 1226 865 298 2930 Fragments arranged by size: 2930 1226 865 763 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4745 882 Size: 617 3246 2219 Fragments arranged by size: 3246 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2392 3265 3873 493 Size: 711 1188 873 608 2702 Fragments arranged by size: 2702 1188 873 711 608 BsaBI GATnn'nnATC Cuts at: 6076 6076 Size: 6082 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3889 4271 4906 275 Size: 53 83 186 936 2356 382 635 1451 Fragments arranged by size: 2356 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4515 4913 5187 5334 5745 4515 Size: 398 274 147 411 4852 Fragments arranged by size: 4852 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2376 2406 1178 Size: 30 165 30 973 30 4854 Fragments arranged by size: 4854 973 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3013 1186 Size: 1827 4255 BseYI C'CCAG_C Cuts at: 3360 5285 5781 3360 Size: 1925 496 3661 Fragments arranged by size: 3661 1925 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2208 1163 Size: 573 472 5037 Fragments arranged by size: 5037 573 472 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3532 4293 5321 1540 Size: 275 186 1531 761 1028 2301 Fragments arranged by size: 2301 1531 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2443 4143 4228 5299 2443 Size: 1700 85 1071 3226 Fragments arranged by size: 3226 1700 1071 85 BsmI GAATG_Cn' Cuts at: 3593 3686 3593 Size: 93 5989 BsmBI CGTCTCn'nnnn_ Cuts at: 2661 2661 Size: 6082 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2389 2428 2443 3401 4143 4228 5299 Size: 399 599 39 15 958 742 85 1071 Cuts at: 5299 1391 Size: 2174 Fragments arranged by size: 2174 1071 958 742 599 399 85 39 15 BspEI T'CCGG_A Cuts at: 5745 5745 Size: 6082 BspHI T'CATG_A Cuts at: 3971 5701 3971 Size: 1730 4352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2449 3437 844 Size: 1605 988 3489 Fragments arranged by size: 3489 1605 988 BsrBI CCG'CTC Cuts at: 1394 1823 3969 1394 Size: 429 2146 3507 Fragments arranged by size: 3507 2146 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3124 4577 4751 65 Size: 1183 1876 1453 174 1396 Fragments arranged by size: 1876 1453 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4726 4913 1700 Size: 110 2916 187 2869 Fragments arranged by size: 2916 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3185 96 Size: 3089 2993 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6082 BssSI C'ACGA_G Cuts at: 4142 5154 4142 Size: 1012 5070 BstAPI GCAn_nnn'nTGC Cuts at: 794 2447 794 Size: 1653 4429 BstBI TT'CG_AA Cuts at: 4886 4886 Size: 6082 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6082 BstYI r'GATC_y Cuts at: 1972 3348 3412 3779 4164 4181 5633 5719 5731 Size: 1376 64 367 385 17 1452 86 12 Cuts at: 5731 6077 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1376 385 367 346 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4591 Fragments arranged by size: 4591 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5343 Fragments arranged by size: 5343 683 56 BtsI GCAGTG_nn' Cuts at: 2762 3598 4392 4412 2762 Size: 836 794 20 4432 Fragments arranged by size: 4432 836 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6082 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6047 DraI TTT'AAA Cuts at: 3386 3386 Size: 6082 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5089 809 Size: 507 3773 1802 Fragments arranged by size: 3773 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3529 4418 6047 Size: 54 1425 50 275 18 1699 889 1629 Cuts at: 6047 8 Size: 43 Fragments arranged by size: 1699 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3529 1537 Size: 275 1717 4090 Fragments arranged by size: 4090 1717 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3209 4012 1353 Size: 1856 803 3423 Fragments arranged by size: 3423 1856 803 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3341 4669 5043 5189 387 Size: 1064 138 1752 1328 374 146 1280 Fragments arranged by size: 1752 1328 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2105 2414 3151 4145 4742 5529 Size: 393 402 71 309 737 994 597 787 Cuts at: 5529 5797 1239 Size: 268 1524 Fragments arranged by size: 1524 994 787 737 597 402 393 309 268 71 EcoRI G'AATT_C Cuts at: 2044 3391 3769 2044 Size: 1347 378 4357 Fragments arranged by size: 4357 1347 378 FspI TGC'GCA Cuts at: 4588 4588 Size: 6082 HaeII r_GCGC'y Cuts at: 1678 5229 1678 Size: 3551 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2531 2563 2658 2690 4723 Size: 32 135 32 959 32 95 32 2033 Cuts at: 4723 4755 4797 4829 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2033 959 135 95 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3127 3426 3672 669 Size: 988 281 1189 299 246 3079 Fragments arranged by size: 3079 1189 988 299 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6082 HpaI GTT'AAC Cuts at: 3672 3672 Size: 6082 KpnI G_GTAC'C Cuts at: 3407 3407 Size: 6082 MfeI C'AATT_G Cuts at: 3681 3681 Size: 6082 MluI A'CGCG_T Cuts at: 5958 5958 Size: 6082 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5196 5380 1305 Size: 3891 184 2007 Fragments arranged by size: 3891 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4260 Fragments arranged by size: 4260 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2930 3200 4040 4399 4558 518 Size: 648 1764 270 840 359 159 2042 Fragments arranged by size: 2042 1764 840 648 359 270 159 MspA1I CmG'CkG Cuts at: 1606 3498 4175 5323 5568 1606 Size: 1892 677 1148 245 2120 Fragments arranged by size: 2120 1892 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4591 Fragments arranged by size: 4591 939 552 NdeI CA'TA_TG Cuts at: 387 3046 387 Size: 2659 3423 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6082 NotI GC'GGCC_GC Cuts at: 3529 3529 Size: 6082 NspI r_CATG'y Cuts at: 1132 1229 3440 4985 1132 Size: 97 2211 1545 2229 Fragments arranged by size: 2229 2211 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6082 PciI A'CATG_T Cuts at: 4981 4981 Size: 6082 PflMI CCAn_nnn'nTGG Cuts at: 1108 3199 1108 Size: 2091 3991 PmeI GTTT'AAAC Cuts at: 3386 3386 Size: 6082 PmlI CAC'GTG Cuts at: 2392 2392 Size: 6082 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4151 4183 5664 5696 4151 Size: 32 1481 32 4537 Fragments arranged by size: 4537 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6082 PsiI TTA'TAA Cuts at: 2663 3652 2663 Size: 989 5093 PstI C_TGCA'G Cuts at: 830 2589 3259 3434 830 Size: 1759 670 175 3478 Fragments arranged by size: 3478 1759 670 175 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6082 SalI G'TCGA_C Cuts at: 1936 3424 1936 Size: 1488 4594 SbfI CC_TGCA'GG Cuts at: 3434 3434 Size: 6082 ScaI AGT'ACT Cuts at: 4330 6042 4330 Size: 1712 4370 SfcI C'TryA_G Cuts at: 826 1045 2585 3255 3430 4565 5246 5437 826 Size: 219 1540 670 175 1135 681 191 1471 Fragments arranged by size: 1540 1471 1135 681 670 219 191 175 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6082 SmaI CCC'GGG Cuts at: 1872 3409 1872 Size: 1537 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2197 2996 4186 5087 5349 820 Size: 197 926 254 799 1190 901 262 1553 Fragments arranged by size: 1553 1190 926 901 799 262 254 197 SnaBI TAC'GTA Cuts at: 493 3873 493 Size: 3380 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6082 SphI G_CATG'C Cuts at: 1132 3440 1132 Size: 2308 3774 SspI AAT'ATT Cuts at: 5 52 2304 4006 5 Size: 47 2252 1702 2081 Fragments arranged by size: 2252 2081 1702 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6082 StyI C'CwwG_G Cuts at: 513 1065 2004 3521 513 Size: 552 939 1517 3074 Fragments arranged by size: 3074 1517 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4109 4126 4279 4464 4909 325 Size: 1255 2529 17 153 185 445 1498 Fragments arranged by size: 2529 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3082 3185 4328 Size: 274 80 33 51 1443 1105 103 1143 Cuts at: 4328 6040 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1143 1105 274 138 103 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2376 2408 6042 6074 2376 Size: 32 3634 32 2384 Fragments arranged by size: 3634 2384 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6082 XbaI T'CTAG_A Cuts at: 3418 3418 Size: 6082 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6082 XmaI C'CCGG_G Cuts at: 1870 3407 1870 Size: 1537 4545 XmnI GAAnn'nnTTC Cuts at: 3097 3218 4211 3097 Size: 121 993 4968 Fragments arranged by size: 4968 993 121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3890 276 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI