################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 532 GTCTAC GT^[AC][GT]AC ApaI 38 GGGCCC GGGCC^C AvaI 439 CCCGGG C^[CT]CG[GA]G BanII 38 GGGCCC G[GA]GC[CT]^C BbsI 279 GTCTTC GAAGAC(2/6) BpuAI 279 GTCTTC GAAGAC(2/6) BsmAI 271 GTCTC GTCTC(1/5) BsmBI 270 CGTCTC CGTCTC(1/5) BsoBI 439 CCCGGG C^[CT]CG[GA]G BssSI 99 CTCGTG CACGAG(-5/-1) BstXI 149 CCATCGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 208 GCGC GCG^C DpnII 220 GATC ^GATC DraIII 376 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 79 CCGTGG C^C[GA][CT]GG EcoRV 252 GATATC GAT^ATC HaeII 207 GGCGCT [GA]GCGC^[CT] HgaI 390 GACGC GACGC(5/10) HhaI 208 GCGC GCG^C HinP1I 208 GCGC G^CGC MaeII 269 ACGT A^CGT MaeIII 10 GTCAC ^GT[ACGT]AC MboI 220 GATC ^GATC MboII 280 TCTTC GAAGA(8/7) NdeII 220 GATC ^GATC NspI 446 ACATGC [GA]CATG^[CT] PpuMI 656 GGGTCCC [GA]G^G[AT]CC[CT] PstI 632 CTGCAG CTGCA^G Sau3AI 220 GATC ^GATC SfaNI 563 GCATC GCATC(5/9) SfcI 632 CTGCAG C^T[GA][CT]AG SmaI 439 CCCGGG CCC^GGG Tsp45I 10 GTCAC ^GT[GC]AC XmaCI 439 CCCGGG C^CCGGG XmaI 439 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 267 GTAC GT^AC 465 GTAC AvaII 508 GGTCC G^G[AT]CC 657 GGTCC BpmI 525 CTCCAG CTGGAG(16/14) 651 CTCCAG BsgI 432 CTGCAC GTGCAG(16/14) 493 GTGCAG BsrI 401 CCAGT ACTGG(1/-1) 527 CCAGT BstF5I 564 CATCC GGATG(2/0) 29 GGATG EcoT14I 614 CCTTGG C^C[AT][AT]GG 641 CCAAGG FokI 564 CATCC GGATG(9/13) 29 GGATG HinfI 8 GAGTC G^A[ACGT]TC 646 GAGTC PleI 8 GAGTC GAGTC(4/5) 646 GAGTC RsaI 267 GTAC GT^AC 465 GTAC StyI 614 CCTTGG C^C[AT][AT]GG 641 CCAAGG TaqI 188 TCGA T^CGA 218 TCGA TspRI 402 CAGTG CA[GC]TG[ACGT][ACGT]^ 528 CAGTG TthHB8I 188 TCGA T^CGA 218 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AlwNI BanI BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HpaII HphI ItaI MnlI MslI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI