################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HphI KasI KpnI KspI MamI MfeI MflI MluI MroI MslI MspA1I MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI StyI SwaI TseI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AciI 761 GCGG CCGC(-3/-1) AclI 740 AACGTT AA^CGTT AflIII 1216 ACATGT A^C[GA][CT]GT AlwI 779 GATCC GGATC(4/5) AvaII 443 GGTCC G^G[AT]CC BalI 64 TGGCCA TGG^CCA BbvCI 1118 CCTCAGC CCTCAGC(-5/-2) BsaWI 730 ACCGGA [AT]^CCGG[AT] BsiYI 434 CCTTTAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 434 CCTTTAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 757 GAATGC GAATGC(1/-1) Bsp1407I 827 TGTACA T^GTACA BspLU11I 1216 ACATGT A^CATGT BsrGI 827 TGTACA T^GTACA Cac8I 356 GCATGC GC[ACGT]^[ACGT]GC Cfr13I 443 GGTCC G^G[ACGT]CC EaeI 64 TGGCCA [CT]^GGCC[GA] Fnu4HI 761 GCGGC GC^[ACGT]GC HaeIII 65 GGCC GG^CC HapII 731 CCGG C^CGG HpaII 731 CCGG C^CGG ItaI 761 GCGGC GC^[ACGT]GC MaeII 741 ACGT A^CGT MaeIII 100 GTGAC ^GT[ACGT]AC MluNI 64 TGGCCA TGG^CCA MscI 64 TGGCCA TGG^CCA MspI 731 CCGG C^CGG NdeI 422 CATATG CA^TATG Psp1406I 740 AACGTT AA^CGTT SapI 428 GAAGAGC GCTCTTC(1/4) Sau96I 443 GGTCC G^G[ACGT]CC SmlI 228 CTTGAG C^T[CT][GA]AG SphI 356 GCATGC GCATG^C SspBI 827 TGTACA T^GTACA SspI 237 AATATT AAT^ATT TaqI 616 TCGA T^CGA Tsp45I 100 GTGAC ^GT[GC]AC TthHB8I 616 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BfaI 395 CTAG C^TAG 1046 CTAG BsmAI 6 GAGAC GTCTC(1/5) 303 GAGAC DpnII 779 GATC ^GATC 1283 GATC DraI 325 TTTAAA TTT^AAA 940 TTTAAA MaeI 395 CTAG C^TAG 1046 CTAG MboI 779 GATC ^GATC 1283 GATC NdeII 779 GATC ^GATC 1283 GATC NspI 356 GCATGC [GA]CATG^[CT] 1216 ACATGT Sau3AI 779 GATC ^GATC 1283 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AcsI AfaI AluI ApoI BpmI BsrI DdeI EarI EcoRI HindIII HinfI Ksp632I MboII MnlI MseI NlaIII RsaI TfiI Tru9I Tsp509I