(Circular) (Six-base) MAPSORT of: pFN21AE3420.seq Check: 8274 from: 1 to: 6154 pFN21AE3420 6154 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3964 278 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3497 1937 Size: 1560 4594 Acc65I G'GTAC_C Cuts at: 3475 3475 Size: 6154 AclI AA'CG_TT Cuts at: 1563 2746 4281 4654 1563 Size: 1183 1535 373 3063 Fragments arranged by size: 3063 1535 1183 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2589 3429 4217 5601 5869 1632 Size: 957 840 788 1384 268 1917 Fragments arranged by size: 1917 1384 957 840 788 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5957 AflIII A'CryG_T Cuts at: 1203 3221 5053 6030 1203 Size: 2018 1832 977 1327 Fragments arranged by size: 2018 1832 1327 977 AgeI A'CCGG_T Cuts at: 4985 4985 Size: 6154 AhdI GACnn_n'nnGTC Cuts at: 4883 4883 Size: 6154 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6154 AlwNI CAG_nnn'CTG Cuts at: 3897 5469 3897 Size: 1572 4582 ApaLI G'TGCA_C Cuts at: 4211 5367 4211 Size: 1156 4998 ApoI r'AATT_y Cuts at: 1493 2140 2280 2287 2407 2475 2559 2658 2895 Size: 647 140 7 120 68 84 99 237 Cuts at: 2895 2979 3231 3315 3463 3689 3841 5950 1493 Size: 84 252 84 148 226 152 2109 1697 Fragments arranged by size: 2109 1697 647 252 237 226 152 148 140 120 99 84 84 84 68 7 AseI AT'TA_AT Cuts at: 160 1032 4708 160 Size: 872 3676 1606 Fragments arranged by size: 3676 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 AvaI C'yCGr_G Cuts at: 1870 1943 3479 1870 Size: 73 1536 4545 Fragments arranged by size: 4545 1536 73 BamHI G'GATC_C Cuts at: 3484 3851 3484 Size: 367 5787 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3475 4930 618 Size: 325 217 627 1688 1455 1842 Fragments arranged by size: 1842 1688 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3473 1391 Size: 2082 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5183 BbvCI CC'TCA_GC Cuts at: 3124 3124 Size: 6154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4345 4379 1903 Size: 34 2408 34 3678 Fragments arranged by size: 3678 2408 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4046 5256 4046 Size: 1210 4944 BclI T'GATC_A Cuts at: 1548 1548 Size: 6154 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4765 136 Size: 107 122 71 631 605 3093 1525 Fragments arranged by size: 3093 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6149 6149 Size: 6154 BlpI GC'TnA_GC Cuts at: 3571 3571 Size: 6154 Bme1580I G_kGCm'C Cuts at: 1790 4215 5371 1790 Size: 2425 1156 2573 Fragments arranged by size: 2573 2425 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2661 3885 4843 Size: 416 53 83 399 24 1244 1224 958 Cuts at: 4843 442 Size: 1753 Fragments arranged by size: 1753 1244 1224 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6154 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3266 3266 Size: 6154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2505 2841 3009 3093 3177 3261 3345 4814 Size: 1266 336 168 84 84 84 84 1469 Cuts at: 4814 1239 Size: 2579 Fragments arranged by size: 2579 1469 1266 336 168 84 84 84 84 Bpu10I CC'TnA_GC Cuts at: 3124 3124 Size: 6154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2254 4279 5144 5442 2254 Size: 2025 865 298 2966 Fragments arranged by size: 2966 2025 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4817 882 Size: 617 3318 2219 Fragments arranged by size: 3318 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3945 493 Size: 711 2741 2702 Fragments arranged by size: 2741 2702 711 BsaBI GATnn'nnATC Cuts at: 6148 6148 Size: 6154 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3961 4343 4978 275 Size: 53 83 186 936 2428 382 635 1451 Fragments arranged by size: 2428 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2735 4587 4985 5259 5406 5817 2735 Size: 1852 398 274 147 411 3072 Fragments arranged by size: 3072 1852 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5929 Fragments arranged by size: 5929 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6154 BseYI C'CCAG_C Cuts at: 5357 5853 5357 Size: 496 5658 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5581 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3604 4365 5393 1540 Size: 275 186 1603 761 1028 2301 Fragments arranged by size: 2301 1603 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4215 4300 5371 4215 Size: 85 1071 4998 Fragments arranged by size: 4998 1071 85 BsmI GAATG_Cn' Cuts at: 2768 3665 3758 2768 Size: 897 93 5164 Fragments arranged by size: 5164 897 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3473 4215 4300 5371 1391 Size: 399 1683 742 85 1071 2174 Fragments arranged by size: 2174 1683 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5817 5817 Size: 6154 BspHI T'CATG_A Cuts at: 4043 5773 4043 Size: 1730 4424 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3509 844 Size: 2665 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4041 1394 Size: 429 2218 3507 Fragments arranged by size: 3507 2218 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4649 4823 65 Size: 1183 3401 174 1396 Fragments arranged by size: 3401 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4798 4985 1700 Size: 110 2988 187 2869 Fragments arranged by size: 2988 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2832 96 Size: 2736 3418 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6154 BssSI C'ACGA_G Cuts at: 4214 5226 4214 Size: 1012 5142 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6154 BstBI TT'CG_AA Cuts at: 4958 4958 Size: 6154 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6154 BstYI r'GATC_y Cuts at: 1972 3484 3851 4236 4253 5705 5791 5803 6149 Size: 1512 367 385 17 1452 86 12 346 Cuts at: 6149 1972 Size: 1977 Fragments arranged by size: 1977 1512 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5602 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5415 Fragments arranged by size: 5415 683 56 BtsI GCAGTG_nn' Cuts at: 3670 4464 4484 3670 Size: 794 20 5340 Fragments arranged by size: 5340 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6119 DraI TTT'AAA Cuts at: 2332 2947 3458 2332 Size: 615 511 5028 Fragments arranged by size: 5028 615 511 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5161 809 Size: 507 3845 1802 Fragments arranged by size: 3845 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2069 3601 4490 Size: 54 1425 50 275 18 239 1532 889 Cuts at: 4490 6119 8 Size: 1629 43 Fragments arranged by size: 1629 1532 1425 889 275 239 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3601 1537 Size: 275 1789 4090 Fragments arranged by size: 4090 1789 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2003 2428 2565 3405 4084 1353 Size: 650 425 137 840 679 3423 Fragments arranged by size: 3423 840 679 650 425 137 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4741 5115 5261 387 Size: 1064 138 3152 374 146 1280 Fragments arranged by size: 3152 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2505 2589 2841 3009 3093 3177 3261 Size: 393 873 84 252 168 84 84 84 Cuts at: 3261 3345 3429 4217 4814 5601 5869 1239 Size: 84 84 788 597 787 268 1524 Fragments arranged by size: 1524 873 788 787 597 393 268 252 168 84 84 84 84 84 84 EcoRI G'AATT_C Cuts at: 2475 2559 2895 3315 3463 3841 2475 Size: 84 336 420 148 378 4788 Fragments arranged by size: 4788 420 378 336 148 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2109 2109 Size: 6154 FspI TGC'GCA Cuts at: 4660 4660 Size: 6154 HaeII r_GCGC'y Cuts at: 1678 5301 1678 Size: 3623 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4795 4827 4869 4901 1373 Size: 32 135 32 3223 32 42 32 2626 Fragments arranged by size: 3223 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3498 3744 669 Size: 988 281 1560 246 3079 Fragments arranged by size: 3079 1560 988 281 246 HindIII A'AGCT_T Cuts at: 748 2708 2772 2940 3024 3360 3444 748 Size: 1960 64 168 84 336 84 3458 Fragments arranged by size: 3458 1960 336 168 84 84 64 HpaI GTT'AAC Cuts at: 3744 3744 Size: 6154 KpnI G_GTAC'C Cuts at: 3479 3479 Size: 6154 MfeI C'AATT_G Cuts at: 3753 3753 Size: 6154 MluI A'CGCG_T Cuts at: 6030 6030 Size: 6154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5268 5452 1305 Size: 3963 184 2007 Fragments arranged by size: 3963 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2071 10 Size: 54 1425 343 239 4093 Fragments arranged by size: 4093 1425 343 239 54 MslI CAynn'nnrTG Cuts at: 518 1166 4112 4471 4630 518 Size: 648 2946 359 159 2042 Fragments arranged by size: 2946 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3570 4247 5395 5640 1606 Size: 1964 677 1148 245 2120 Fragments arranged by size: 2120 1964 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5602 NdeI CA'TA_TG Cuts at: 387 2428 387 Size: 2041 4113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6154 NotI GC'GGCC_GC Cuts at: 3601 3601 Size: 6154 NspI r_CATG'y Cuts at: 1132 1229 2365 3225 3512 5057 1132 Size: 97 1136 860 287 1545 2229 Fragments arranged by size: 2229 1545 1136 860 287 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6154 PciI A'CATG_T Cuts at: 3221 5053 3221 Size: 1832 4322 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6154 PmeI GTTT'AAAC Cuts at: 3458 3458 Size: 6154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4223 4255 5736 5768 4223 Size: 32 1481 32 4609 Fragments arranged by size: 4609 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6154 PsiI TTA'TAA Cuts at: 3724 3724 Size: 6154 PstI C_TGCA'G Cuts at: 830 3506 830 Size: 2676 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6154 SalI G'TCGA_C Cuts at: 1936 3496 1936 Size: 1560 4594 SapI GCTCTTCn'nnn_ Cuts at: 2428 2428 Size: 6154 SbfI CC_TGCA'GG Cuts at: 3506 3506 Size: 6154 ScaI AGT'ACT Cuts at: 4402 6114 4402 Size: 1712 4442 SfcI C'TryA_G Cuts at: 826 1045 3502 4637 5318 5509 826 Size: 219 2457 1135 681 191 1471 Fragments arranged by size: 2457 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6154 SmaI CCC'GGG Cuts at: 1872 3481 1872 Size: 1609 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2233 4258 5159 5421 820 Size: 197 926 290 2025 901 262 1553 Fragments arranged by size: 2025 1553 926 901 290 262 197 SnaBI TAC'GTA Cuts at: 493 3945 493 Size: 3452 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6154 SphI G_CATG'C Cuts at: 1132 2365 3512 1132 Size: 1233 1147 3774 Fragments arranged by size: 3774 1233 1147 SspI AAT'ATT Cuts at: 5 52 2244 4078 5 Size: 47 2192 1834 2081 Fragments arranged by size: 2192 2081 1834 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6154 StyI C'CwwG_G Cuts at: 513 1065 3593 513 Size: 552 2528 3074 Fragments arranged by size: 3074 2528 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4181 4198 4351 4536 4981 325 Size: 1255 2601 17 153 185 445 1498 Fragments arranged by size: 2601 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2808 2832 3218 Size: 274 80 33 51 1443 831 24 386 Cuts at: 3218 3420 4400 6112 96 Size: 202 980 1712 138 Fragments arranged by size: 1712 1443 980 831 386 274 202 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6114 6146 6114 Size: 32 6122 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6154 XbaI T'CTAG_A Cuts at: 3490 3490 Size: 6154 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6154 XmaI C'CCGG_G Cuts at: 1870 3479 1870 Size: 1609 4545 XmnI GAAnn'nnTTC Cuts at: 4283 4283 Size: 6154 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3962 276 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI