(Circular) (Six-base) MAPSORT of: pFN21AE4242.seq Check: 7333 from: 1 to: 7417 pFN21AE4242 7417 bp With 182 enzymes: SgfI * March 28, 2012 13:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5227 278 Size: 53 83 186 936 3691 2468 Fragments arranged by size: 3691 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4760 1937 Size: 2823 4594 Acc65I G'GTAC_C Cuts at: 4738 4738 Size: 7417 AclI AA'CG_TT Cuts at: 1563 5544 5917 1563 Size: 3981 373 3063 Fragments arranged by size: 3981 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3129 3174 4088 4598 5480 6864 7132 1632 Size: 1497 45 914 510 882 1384 268 1917 Fragments arranged by size: 1917 1497 1384 914 882 510 268 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7220 AflIII A'CryG_T Cuts at: 1203 6316 7293 1203 Size: 5113 977 1327 Fragments arranged by size: 5113 1327 977 AgeI A'CCGG_T Cuts at: 6248 6248 Size: 7417 AhdI GACnn_n'nnGTC Cuts at: 3619 6146 3619 Size: 2527 4890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7417 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7417 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3201 3233 4649 4681 3201 Size: 32 1416 32 5937 Fragments arranged by size: 5937 1416 32 32 AlwNI CAG_nnn'CTG Cuts at: 3757 5160 6732 3757 Size: 1403 1572 4442 Fragments arranged by size: 4442 1572 1403 ApaI G_GGCC'C Cuts at: 3929 3929 Size: 7417 ApaLI G'TGCA_C Cuts at: 5474 6630 5474 Size: 1156 6261 ApoI r'AATT_y Cuts at: 1493 2138 2571 3094 3646 4726 4952 5104 7213 Size: 645 433 523 552 1080 226 152 2109 Cuts at: 7213 1493 Size: 1697 Fragments arranged by size: 2109 1697 1080 645 552 523 433 226 152 AseI AT'TA_AT Cuts at: 160 1032 3663 5971 160 Size: 872 2631 2308 1606 Fragments arranged by size: 2631 2308 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7417 AvaI C'yCGr_G Cuts at: 1870 1943 4742 1870 Size: 73 2799 4545 Fragments arranged by size: 4545 2799 73 AvrII C'CTAG_G Cuts at: 2474 2474 Size: 7417 BamHI G'GATC_C Cuts at: 3314 4747 5114 3314 Size: 1433 367 5617 Fragments arranged by size: 5617 1433 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4738 6193 618 Size: 325 217 627 2951 1455 1842 Fragments arranged by size: 2951 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3929 4736 1391 Size: 2538 807 4072 Fragments arranged by size: 4072 2538 807 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3606 928 Size: 971 1707 4739 Fragments arranged by size: 4739 1707 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5608 5642 1903 Size: 34 3671 34 3678 Fragments arranged by size: 3678 3671 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3441 5309 6519 3441 Size: 1868 1210 4339 Fragments arranged by size: 4339 1868 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7417 BfrBI ATG'CAT Cuts at: 4604 4604 Size: 7417 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6028 136 Size: 107 122 71 631 605 4356 1525 Fragments arranged by size: 4356 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2846 7412 2846 Size: 4566 2851 BlpI GC'TnA_GC Cuts at: 3629 4103 4834 3629 Size: 474 731 6212 Fragments arranged by size: 6212 731 474 Bme1580I G_kGCm'C Cuts at: 1790 3189 3929 4490 5478 6634 1790 Size: 1399 740 561 988 1156 2573 Fragments arranged by size: 2573 1399 1156 988 740 561 BmgBI CAC'GTC Cuts at: 1318 3372 1318 Size: 2054 5363 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3276 5148 6106 Size: 416 53 83 399 24 1859 1872 958 Cuts at: 6106 442 Size: 1753 Fragments arranged by size: 1872 1859 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7417 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2558 3665 3689 6077 1239 Size: 1319 1107 24 2388 2579 Fragments arranged by size: 2579 2388 1319 1107 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5542 6407 6705 5542 Size: 865 298 6254 Fragments arranged by size: 6254 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6080 882 Size: 617 4581 2219 Fragments arranged by size: 4581 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2594 3172 3748 5208 493 Size: 711 1390 578 576 1460 2702 Fragments arranged by size: 2702 1460 1390 711 578 576 BsaBI GATnn'nnATC Cuts at: 7411 7411 Size: 7417 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5224 5606 6241 275 Size: 53 83 186 936 3691 382 635 1451 Fragments arranged by size: 3691 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5850 6248 6522 6669 7080 5850 Size: 398 274 147 411 6187 Fragments arranged by size: 6187 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3936 3966 1178 Size: 30 165 30 2533 30 4629 Fragments arranged by size: 4629 2533 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7417 BseYI C'CCAG_C Cuts at: 3225 3310 3928 6620 7116 3225 Size: 85 618 2692 496 3526 Fragments arranged by size: 3526 2692 618 496 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3770 4054 1163 Size: 573 2034 284 4526 Fragments arranged by size: 4526 2034 573 284 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4867 5628 6656 1540 Size: 275 186 2866 761 1028 2301 Fragments arranged by size: 2866 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5478 5563 6634 5478 Size: 85 1071 6261 Fragments arranged by size: 6261 1071 85 BsmI GAATG_Cn' Cuts at: 4052 4606 4928 5021 4052 Size: 554 322 93 6448 Fragments arranged by size: 6448 554 322 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3189 3929 4490 4736 5478 5563 6634 Size: 399 1399 740 561 246 742 85 1071 Cuts at: 6634 1391 Size: 2174 Fragments arranged by size: 2174 1399 1071 742 740 561 399 246 85 BspEI T'CCGG_A Cuts at: 7080 7080 Size: 7417 BspHI T'CATG_A Cuts at: 5306 7036 5306 Size: 1730 5687 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4772 844 Size: 3928 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5304 1394 Size: 429 3481 3507 Fragments arranged by size: 3507 3481 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2439 4384 4536 5912 6086 65 Size: 1183 1191 1945 152 1376 174 1396 Fragments arranged by size: 1945 1396 1376 1191 1183 174 152 BsrFI r'CCGG_y Cuts at: 1700 1810 6061 6248 1700 Size: 110 4251 187 2869 Fragments arranged by size: 4251 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7417 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7417 BssSI C'ACGA_G Cuts at: 5477 6489 5477 Size: 1012 6405 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7417 BstBI TT'CG_AA Cuts at: 4687 6221 4687 Size: 1534 5883 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7417 BstYI r'GATC_y Cuts at: 1972 2846 3314 4747 5114 5499 5516 6968 7054 Size: 874 468 1433 367 385 17 1452 86 Cuts at: 7054 7066 7412 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1433 874 468 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2561 2561 Size: 7417 BtgI C'CryG_G Cuts at: 513 1065 2004 3498 4204 513 Size: 552 939 1494 706 3726 Fragments arranged by size: 3726 1494 939 706 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3054 487 Size: 683 56 1828 4850 Fragments arranged by size: 4850 1828 683 56 BtsI GCAGTG_nn' Cuts at: 4218 4933 5727 5747 4218 Size: 715 794 20 5888 Fragments arranged by size: 5888 794 715 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7417 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3253 3288 4207 4242 529 Size: 35 2689 35 919 35 3704 Fragments arranged by size: 3704 2689 919 35 35 35 DraI TTT'AAA Cuts at: 3645 4721 3645 Size: 1076 6341 DraIII CAC_nnn'GTG Cuts at: 3940 3940 Size: 7417 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6424 809 Size: 507 5108 1802 Fragments arranged by size: 5108 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 2864 3147 Size: 54 1425 50 275 18 390 644 283 Cuts at: 3147 4864 5753 7382 8 Size: 1717 889 1629 43 Fragments arranged by size: 1717 1629 1425 889 644 390 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4864 1537 Size: 275 3052 4090 Fragments arranged by size: 4090 3052 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3343 3449 4064 4460 5347 1353 Size: 1990 106 615 396 887 3423 Fragments arranged by size: 3423 1990 887 615 396 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6004 6378 6524 387 Size: 1064 138 4415 374 146 1280 Fragments arranged by size: 4415 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2558 3129 3174 3665 3689 4088 4598 Size: 393 926 571 45 491 24 399 510 Cuts at: 4598 5480 6077 6864 7132 1239 Size: 882 597 787 268 1524 Fragments arranged by size: 1524 926 882 787 597 571 510 491 399 393 268 45 24 EcoO109I rG'GnC_Cy Cuts at: 3331 3925 3331 Size: 594 6823 EcoRI G'AATT_C Cuts at: 2138 4726 5104 2138 Size: 2588 378 4451 Fragments arranged by size: 4451 2588 378 EcoRV GAT'ATC Cuts at: 4352 4352 Size: 7417 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4169 4428 4169 Size: 259 7158 FspI TGC'GCA Cuts at: 3537 5923 3537 Size: 2386 5031 HaeII r_GCGC'y Cuts at: 1678 2434 6564 1678 Size: 756 4130 2531 Fragments arranged by size: 4130 2531 756 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2831 2863 3605 3637 3859 Size: 32 135 32 1259 32 742 32 222 Cuts at: 3859 3891 3936 3968 4280 4312 6058 6090 6132 Size: 32 45 32 312 32 1746 32 42 Cuts at: 6132 6164 1373 Size: 32 2626 Fragments arranged by size: 2626 1746 1259 742 312 222 135 45 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4201 4761 5007 669 Size: 988 281 2263 560 246 3079 Fragments arranged by size: 3079 2263 988 560 281 246 HindIII A'AGCT_T Cuts at: 748 2718 748 Size: 1970 5447 HpaI GTT'AAC Cuts at: 5007 5007 Size: 7417 KpnI G_GTAC'C Cuts at: 4742 4742 Size: 7417 MfeI C'AATT_G Cuts at: 4224 5016 4224 Size: 792 6625 MluI A'CGCG_T Cuts at: 7293 7293 Size: 7417 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2426 6531 6715 1305 Size: 1121 4105 184 2007 Fragments arranged by size: 4105 2007 1121 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2222 3149 10 Size: 54 1425 343 390 927 4278 Fragments arranged by size: 4278 1425 927 390 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2835 3503 4280 4389 4434 5375 5734 Size: 648 1669 668 777 109 45 941 359 Cuts at: 5734 5893 518 Size: 159 2042 Fragments arranged by size: 2042 1669 941 777 668 648 359 159 109 45 MspA1I CmG'CkG Cuts at: 1606 2306 2319 3229 3427 4833 5510 6658 6903 Size: 700 13 910 198 1406 677 1148 245 Cuts at: 6903 1606 Size: 2120 Fragments arranged by size: 2120 1406 1148 910 700 677 245 198 13 NcoI C'CATG_G Cuts at: 513 1065 2004 3498 513 Size: 552 939 1494 4432 Fragments arranged by size: 4432 1494 939 552 NdeI CA'TA_TG Cuts at: 387 2854 387 Size: 2467 4950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7417 NotI GC'GGCC_GC Cuts at: 4864 4864 Size: 7417 NsiI A_TGCA'T Cuts at: 4606 4606 Size: 7417 NspI r_CATG'y Cuts at: 1132 1229 4199 4775 6320 1132 Size: 97 2970 576 1545 2229 Fragments arranged by size: 2970 2229 1545 576 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7417 PciI A'CATG_T Cuts at: 6316 6316 Size: 7417 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7417 PfoI T'CCnGG_A Cuts at: 2888 2888 Size: 7417 PmeI GTTT'AAAC Cuts at: 4721 4721 Size: 7417 PmlI CAC'GTG Cuts at: 3172 3172 Size: 7417 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5486 5518 6999 7031 5486 Size: 32 1481 32 5872 Fragments arranged by size: 5872 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7417 PsiI TTA'TAA Cuts at: 2096 4987 2096 Size: 2891 4526 PspOMI G'GGCC_C Cuts at: 3925 3925 Size: 7417 PstI C_TGCA'G Cuts at: 830 3453 3918 4192 4769 830 Size: 2623 465 274 577 3478 Fragments arranged by size: 3478 2623 577 465 274 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7417 PvuII CAG'CTG Cuts at: 2319 3229 3427 2319 Size: 910 198 6309 Fragments arranged by size: 6309 910 198 SalI G'TCGA_C Cuts at: 1936 4759 1936 Size: 2823 4594 SapI GCTCTTCn'nnn_ Cuts at: 3449 3449 Size: 7417 SbfI CC_TGCA'GG Cuts at: 4769 4769 Size: 7417 ScaI AGT'ACT Cuts at: 4683 5665 7377 4683 Size: 982 1712 4723 Fragments arranged by size: 4723 1712 982 SfcI C'TryA_G Cuts at: 826 1045 2238 3365 3449 3863 3914 4188 4644 Size: 219 1193 1127 84 414 51 274 456 Cuts at: 4644 4765 5900 6581 6772 826 Size: 121 1135 681 191 1471 Fragments arranged by size: 1471 1193 1135 1127 681 456 414 274 219 191 121 84 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7417 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7417 SmaI CCC'GGG Cuts at: 1872 4744 1872 Size: 2872 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5521 6422 6684 820 Size: 197 926 3578 901 262 1553 Fragments arranged by size: 3578 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2594 5208 493 Size: 2101 2614 2702 Fragments arranged by size: 2702 2614 2101 SpeI A'CTAG_T Cuts at: 152 152 Size: 7417 SphI G_CATG'C Cuts at: 1132 4775 1132 Size: 3643 3774 SspI AAT'ATT Cuts at: 5 52 2776 3033 4576 5341 5 Size: 47 2724 257 1543 765 2081 Fragments arranged by size: 2724 2081 1543 765 257 47 StuI AGG'CCT Cuts at: 1517 3106 1517 Size: 1589 5828 StyI C'CwwG_G Cuts at: 513 1065 2004 2474 2624 2967 3070 3190 3498 Size: 552 939 470 150 343 103 120 308 Cuts at: 3498 4856 513 Size: 1358 3074 Fragments arranged by size: 3074 1358 939 552 470 343 308 150 120 103 SwaI ATTT'AAAT Cuts at: 3645 3645 Size: 7417 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2617 5444 5461 5614 5799 6244 325 Size: 1255 1037 2827 17 153 185 445 1498 Fragments arranged by size: 2827 1498 1255 1037 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4681 5663 7375 Size: 274 80 33 51 1443 2704 982 1712 Cuts at: 7375 96 Size: 138 Fragments arranged by size: 2704 1712 1443 982 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3129 3161 7377 7409 3129 Size: 32 4216 32 3137 Fragments arranged by size: 4216 3137 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3373 1316 Size: 2057 5360 XbaI T'CTAG_A Cuts at: 3892 4753 3892 Size: 861 6556 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4629 4629 Size: 7417 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7417 XmaI C'CCGG_G Cuts at: 1870 4742 1870 Size: 2872 4545 XmnI GAAnn'nnTTC Cuts at: 2788 2976 4575 5546 2788 Size: 188 1599 971 4659 Fragments arranged by size: 4659 1599 971 188 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5225 276 Size: 53 83 186 936 3691 2468 Fragments arranged by size: 3691 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI