(Linear) (Six-base) MAPSORT of: KIEE4242.seq Check: 884 from: 1 to: 2712 KIEE4242.orf With 182 enzymes: SgfI * March 22, 2012 17:37 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1124 1169 2083 2593 2712 Size: 1124 45 914 510 119 Fragments arranged by size: 1124 914 510 119 45 AhdI GACnn_n'nnGTC Cuts at: 0 1614 2712 Size: 1614 1098 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1196 1228 2644 2676 2712 Size: 1196 32 1416 32 36 Fragments arranged by size: 1416 1196 36 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1752 2712 Size: 1752 960 ApaI G_GGCC'C Cuts at: 0 1924 2712 Size: 1924 788 ApoI r'AATT_y Cuts at: 0 133 566 1089 1641 2712 Size: 133 433 523 552 1071 Fragments arranged by size: 1071 552 523 433 133 AseI AT'TA_AT Cuts at: 0 1658 2712 Size: 1658 1054 AvrII C'CTAG_G Cuts at: 0 469 2712 Size: 469 2243 BamHI G'GATC_C Cuts at: 0 1309 2712 Size: 1309 1403 BanII G_rGCy'C Cuts at: 0 1924 2712 Size: 1924 788 BbsI GAAGACnn'nnnn_ Cuts at: 0 1601 2712 Size: 1601 1111 BciVI GTATCCnnnnn_n' Cuts at: 0 1436 2712 Size: 1436 1276 BfrBI ATG'CAT Cuts at: 0 2599 2712 Size: 2599 113 BglII A'GATC_T Cuts at: 0 841 2712 Size: 841 1871 BlpI GC'TnA_GC Cuts at: 0 1624 2098 2712 Size: 1624 474 614 Fragments arranged by size: 1624 614 474 Bme1580I G_kGCm'C Cuts at: 0 1184 1924 2485 2712 Size: 1184 740 561 227 Fragments arranged by size: 1184 740 561 227 BmgBI CAC'GTC Cuts at: 0 1367 2712 Size: 1367 1345 BmrI ACTGGGnnnn_n' Cuts at: 0 1271 2712 Size: 1271 1441 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 553 1660 1684 2712 Size: 553 1107 24 1028 Fragments arranged by size: 1107 1028 553 24 BsaAI yAC'GTr Cuts at: 0 589 1167 1743 2712 Size: 589 578 576 969 Fragments arranged by size: 969 589 578 576 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1931 1961 2712 Size: 1931 30 751 Fragments arranged by size: 1931 751 30 BseYI C'CCAG_C Cuts at: 0 1220 1305 1923 2712 Size: 1220 85 618 789 Fragments arranged by size: 1220 789 618 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1765 2049 2712 Size: 1765 284 663 Fragments arranged by size: 1765 663 284 BsmI GAATG_Cn' Cuts at: 0 2047 2601 2712 Size: 2047 554 111 Fragments arranged by size: 2047 554 111 Bsp1286I G_dGCh'C Cuts at: 0 1184 1924 2485 2712 Size: 1184 740 561 227 Fragments arranged by size: 1184 740 561 227 BsrDI GCAATG_nn' Cuts at: 0 434 2379 2531 2712 Size: 434 1945 152 181 Fragments arranged by size: 1945 434 181 152 BstBI TT'CG_AA Cuts at: 0 2682 2712 Size: 2682 30 BstYI r'GATC_y Cuts at: 0 841 1309 2712 Size: 841 468 1403 Fragments arranged by size: 1403 841 468 Bsu36I CC'TnA_GG Cuts at: 0 556 2712 Size: 556 2156 BtgI C'CryG_G Cuts at: 0 1493 2199 2712 Size: 1493 706 513 Fragments arranged by size: 1493 706 513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1049 2712 Size: 1049 1663 BtsI GCAGTG_nn' Cuts at: 0 2213 2712 Size: 2213 499 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1248 1283 2202 2237 2712 Size: 1248 35 919 35 475 Fragments arranged by size: 1248 919 475 35 35 DraI TTT'AAA Cuts at: 0 1640 2712 Size: 1640 1072 DraIII CAC_nnn'GTG Cuts at: 0 1935 2712 Size: 1935 777 EaeI y'GGCC_r Cuts at: 0 215 859 1142 2712 Size: 215 644 283 1570 Fragments arranged by size: 1570 644 283 215 EarI CTCTTCn'nnn_ Cuts at: 0 1338 1444 2059 2455 2712 Size: 1338 106 615 396 257 Fragments arranged by size: 1338 615 396 257 106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 553 1124 1169 1660 1684 2083 2593 2712 Size: 553 571 45 491 24 399 510 119 Fragments arranged by size: 571 553 510 491 399 119 45 24 EcoO109I rG'GnC_Cy Cuts at: 0 1326 1920 2712 Size: 1326 594 792 Fragments arranged by size: 1326 792 594 EcoRI G'AATT_C Cuts at: 0 133 2712 Size: 133 2579 EcoRV GAT'ATC Cuts at: 0 2347 2712 Size: 2347 365 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2164 2423 2712 Size: 2164 259 289 Fragments arranged by size: 2164 289 259 FspI TGC'GCA Cuts at: 0 1532 2712 Size: 1532 1180 HaeII r_GCGC'y Cuts at: 0 429 2712 Size: 429 2283 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 826 858 1600 1632 1854 1886 1931 1963 Size: 826 32 742 32 222 32 45 32 Cuts at: 1963 2275 2307 2712 Size: 312 32 405 Fragments arranged by size: 826 742 405 312 222 45 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2196 2712 Size: 2196 516 HindIII A'AGCT_T Cuts at: 0 713 2712 Size: 713 1999 MfeI C'AATT_G Cuts at: 0 2219 2712 Size: 2219 493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 421 2712 Size: 421 2291 MscI TGG'CCA Cuts at: 0 217 1144 2712 Size: 217 927 1568 Fragments arranged by size: 1568 927 217 MslI CAynn'nnrTG Cuts at: 0 830 1498 2275 2384 2429 2712 Size: 830 668 777 109 45 283 Fragments arranged by size: 830 777 668 283 109 45 MspA1I CmG'CkG Cuts at: 0 301 314 1224 1422 2712 Size: 301 13 910 198 1290 Fragments arranged by size: 1290 910 301 198 13 NcoI C'CATG_G Cuts at: 0 1493 2712 Size: 1493 1219 NdeI CA'TA_TG Cuts at: 0 849 2712 Size: 849 1863 NsiI A_TGCA'T Cuts at: 0 2601 2712 Size: 2601 111 NspI r_CATG'y Cuts at: 0 2194 2712 Size: 2194 518 PfoI T'CCnGG_A Cuts at: 0 883 2712 Size: 883 1829 PmlI CAC'GTG Cuts at: 0 1167 2712 Size: 1167 1545 PsiI TTA'TAA Cuts at: 0 91 2712 Size: 91 2621 PspOMI G'GGCC_C Cuts at: 0 1920 2712 Size: 1920 792 PstI C_TGCA'G Cuts at: 0 1448 1913 2187 2712 Size: 1448 465 274 525 Fragments arranged by size: 1448 525 465 274 PvuII CAG'CTG Cuts at: 0 314 1224 1422 2712 Size: 314 910 198 1290 Fragments arranged by size: 1290 910 314 198 SapI GCTCTTCn'nnn_ Cuts at: 0 1444 2712 Size: 1444 1268 ScaI AGT'ACT Cuts at: 0 2678 2712 Size: 2678 34 SfcI C'TryA_G Cuts at: 0 233 1360 1444 1858 1909 2183 2639 2712 Size: 233 1127 84 414 51 274 456 73 Fragments arranged by size: 1127 456 414 274 233 84 73 51 SnaBI TAC'GTA Cuts at: 0 589 2712 Size: 589 2123 SspI AAT'ATT Cuts at: 0 771 1028 2571 2712 Size: 771 257 1543 141 Fragments arranged by size: 1543 771 257 141 StuI AGG'CCT Cuts at: 0 1101 2712 Size: 1101 1611 StyI C'CwwG_G Cuts at: 0 469 619 962 1065 1185 1493 2712 Size: 469 150 343 103 120 308 1219 Fragments arranged by size: 1219 469 343 308 150 120 103 SwaI ATTT'AAAT Cuts at: 0 1640 2712 Size: 1640 1072 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 612 2712 Size: 612 2100 TatI w'GTAC_w Cuts at: 0 2676 2712 Size: 2676 36 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1124 1156 2712 Size: 1124 32 1556 Fragments arranged by size: 1556 1124 32 Tth111I GACn'n_nGTC Cuts at: 0 1368 2712 Size: 1368 1344 XbaI T'CTAG_A Cuts at: 0 1887 2712 Size: 1887 825 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2624 2712 Size: 2624 88 XmnI GAAnn'nnTTC Cuts at: 0 783 971 2570 2712 Size: 783 188 1599 142 Fragments arranged by size: 1599 783 188 142 Enzymes that do cut: AcuI AhdI AloI AlwNI ApaI ApoI AseI AvrII BamHI BanII BbsI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BpmI BsaAI BsaXI BseYI BsgI BsmI Bsp1286I BsrDI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PfoI PmlI PsiI PspOMI PstI PvuII SapI ScaI SfcI SnaBI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI ApaLI AscI AsiSI AvaI BaeI BanI BbeI BbvCI BcgI BclI BglI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I ClaI DrdI EagI EciI EcoICRI EcoNI FseI FspAI HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI XhoI XmaI ZraI