################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AclI AcsI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AvrII BbsI BbvCI BclI BlnI BpuAI BsaBI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspHI BsrGI BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI DraI DsaI Eam1105I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KspI MamI MfeI MluI MunI NcoI NdeI NheI NruI NsiI NspV PacI PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 85 GGTACC G^GTACC AccI 1390 GTCTAC GT^[AC][GT]AC AccIII 1476 TCCGGA T^CCGGA AflII 454 CTTAAG C^TTAAG AflIII 1941 ACATGT A^C[GA][CT]GT AgeI 75 ACCGGT A^CCGGT Asp718I 85 GGTACC G^GTACC BamHI 2031 GGATCC G^GATCC BbrPI 1210 CACGTG CAC^GTG BcgI 1590 CGAGATGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1473 GTATCC GTATCC(6/5) BfaI 898 CTAG C^TAG BfrI 454 CTTAAG C^TTAAG BglII 275 AGATCT A^GATCT BlpI 875 GCTCAGC GC^T[ACGT]AGC Bpu1102I 875 GCTCAGC GC^T[ACGT]AGC BsaAI 1210 CACGTG [CT]AC^GT[GA] BseAI 1476 TCCGGA T^CCGGA BsmBI 296 GAGACG CGTCTC(1/5) BspEI 1476 TCCGGA T^CCGGA BspLU11I 1941 ACATGT A^CATGT BsrDI 290 CATTGC GCAATG(2/0) BsrI 649 CCAGT ACTGG(1/-1) BstEII 1051 GGTCACC G^GT[ACGT]ACC BstPI 1051 GGTCACC G^GT[ACGT]ACC BstXI 724 CCAGGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 875 GCTCAGC GC^T[ACGT]AGC CpoI 72 CGGACCG CG^G[AT]CCG DraIII 475 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2053 GACGTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1051 GGTCACC G^GT[ACGT]ACC KpnI 85 GGTACC GGTAC^C MaeI 898 CTAG C^TAG MroI 1476 TCCGGA T^CCGGA PaeR7I 1291 CTCGAG C^TCGAG PflMI 630 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 75 ACCGGT A^CCGGT PmaCI 1210 CACGTG CAC^GTG PmlI 1210 CACGTG CAC^GTG PstI 1505 CTGCAG CTGCA^G RsrII 72 CGGACCG CG^G[AT]CCG SfcI 1505 CTGCAG C^T[GA][CT]AG Tsp509I 1448 AATT ^AATT Van91I 630 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1291 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AatII 298 GACGTC GACGT^C 2059 GACGTC Aor51HI 404 AGCGCT AGC^GCT 1295 AGCGCT ApaI 639 GGGCCC GGGCC^C 1151 GGGCCC AviII 146 TGCGCA TGC^GCA 1260 TGCGCA BalI 1544 TGGCCA TGG^CCA 1962 TGGCCA BglI 1645 GCCGTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1955 GCCACCCTGGC BsaI 565 GGTCTC GGTCTC(1/5) 1327 GGTCTC BsaWI 75 ACCGGT [AT]^CCGG[AT] 1476 TCCGGA BssHII 1218 GCGCGC G^CGCGC 1301 GCGCGC EarI 693 CTCTTC CTCTTC(1/4) 1189 GAAGAG Eco47III 404 AGCGCT AGC^GCT 1295 AGCGCT EcoT14I 341 CCTTGG C^C[AT][AT]GG 534 CCAAGG FspI 146 TGCGCA TGC^GCA 1260 TGCGCA Ksp632I 693 CTCTTC CTCTTC(1/4) 1189 GAAGAG MaeIII 1052 GTCAC ^GT[ACGT]AC 2051 GTGAC MluNI 1544 TGGCCA TGG^CCA 1962 TGGCCA MscI 1544 TGGCCA TGG^CCA 1962 TGGCCA MseI 208 TTAA T^TAA 455 TTAA MslI 27 CACCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1799 CACTCAAGTG NotI 913 GCGGCCGC GC^GGCCGC 1510 GCGGCCGC NspI 1772 ACATGC [GA]CATG^[CT] 1941 ACATGT PpuMI 1109 GGGACCC [GA]G^G[AT]CC[CT] 1928 GGGACCT StuI 656 AGGCCT AGG^CCT 689 AGGCCT StyI 341 CCTTGG C^C[AT][AT]GG 534 CCAAGG Tru9I 208 TTAA T^TAA 455 TTAA Tsp45I 1052 GTCAC ^GT[GC]AC 2051 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfoI SmaI SmlI TaqI TseI TspRI TthHB8I XhoII XmaCI XmaI