(Circular) (Six-base) MAPSORT of: pFN21AE5727.seq Check: 7054 from: 1 to: 6826 pFN21AE5727 6826 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2478 3375 2478 Size: 897 5929 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2307 4068 4636 278 Size: 53 83 186 936 771 1761 568 2468 Fragments arranged by size: 2468 1761 936 771 568 186 83 53 AccI GT'mk_AC Cuts at: 1937 3396 4169 1937 Size: 1459 773 4594 Fragments arranged by size: 4594 1459 773 Acc65I G'GTAC_C Cuts at: 2090 4147 2090 Size: 2057 4769 AclI AA'CG_TT Cuts at: 1563 4953 5326 1563 Size: 3390 373 3063 Fragments arranged by size: 3390 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3484 3494 4889 6273 6541 1632 Size: 1852 10 1395 1384 268 1917 Fragments arranged by size: 1917 1852 1395 1384 268 10 AfeI AGC'GCT Cuts at: 2411 3302 2411 Size: 891 5935 AflII C'TTAA_G Cuts at: 820 1017 2459 820 Size: 197 1442 5187 Fragments arranged by size: 5187 1442 197 AflIII A'CryG_T Cuts at: 1203 3946 5725 6702 1203 Size: 2743 1779 977 1327 Fragments arranged by size: 2743 1779 1327 977 AgeI A'CCGG_T Cuts at: 2080 5657 2080 Size: 3577 3249 AhdI GACnn_n'nnGTC Cuts at: 5555 5555 Size: 6826 AleI CACnn'nnGTG Cuts at: 1166 2036 3808 1166 Size: 870 1772 4184 Fragments arranged by size: 4184 1772 870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3383 1184 Size: 2199 4627 AlwNI CAG_nnn'CTG Cuts at: 2248 2558 3589 3976 4569 6141 2248 Size: 310 1031 387 593 1572 2933 Fragments arranged by size: 2933 1572 1031 593 387 310 ApaI G_GGCC'C Cuts at: 2648 3160 2648 Size: 512 6314 ApaLI G'TGCA_C Cuts at: 4883 6039 4883 Size: 1156 5670 ApoI r'AATT_y Cuts at: 1493 4135 4361 4513 6622 1493 Size: 2642 226 152 2109 1697 Fragments arranged by size: 2642 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5380 160 Size: 872 4348 1606 Fragments arranged by size: 4348 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 AvaI C'yCGr_G Cuts at: 1870 1943 2132 2799 3020 3296 3325 3631 4000 Size: 73 189 667 221 276 29 306 369 Cuts at: 4000 4094 4151 1870 Size: 94 57 4545 Fragments arranged by size: 4545 667 369 306 276 221 189 94 73 57 29 BamHI G'GATC_C Cuts at: 4036 4156 4523 4036 Size: 120 367 6339 Fragments arranged by size: 6339 367 120 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 2090 2507 2543 2590 Size: 325 217 627 296 7 417 36 47 Cuts at: 2590 3015 3668 4004 4147 5602 618 Size: 425 653 336 143 1455 1842 Fragments arranged by size: 1842 1455 653 627 425 417 336 325 296 217 143 47 36 7 BanII G_rGCy'C Cuts at: 1391 2133 2192 2648 3160 3768 4014 4145 1391 Size: 742 59 456 512 608 246 131 4072 Fragments arranged by size: 4072 742 608 512 456 246 131 59 BbeI G_GCGC'C Cuts at: 2511 2547 2594 3019 2511 Size: 36 47 425 6318 Fragments arranged by size: 6318 425 47 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5855 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3584 3618 5017 5051 1903 Size: 34 50 34 1563 34 1399 34 3678 Fragments arranged by size: 3678 1563 1399 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3489 4718 5928 3489 Size: 1229 1210 4387 Fragments arranged by size: 4387 1229 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6826 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3656 3966 5437 Size: 107 122 71 631 605 1984 310 1471 Cuts at: 5437 136 Size: 1525 Fragments arranged by size: 1984 1525 1471 631 605 310 122 107 71 BglII A'GATC_T Cuts at: 2280 6821 2280 Size: 4541 2285 BlpI GC'TnA_GC Cuts at: 2881 4243 2881 Size: 1362 5464 Bme1580I G_kGCm'C Cuts at: 1790 2648 2779 2814 3160 4887 6043 1790 Size: 858 131 35 346 1727 1156 2573 Fragments arranged by size: 2573 1727 1156 858 346 131 35 BmgBI CAC'GTC Cuts at: 1318 4060 1318 Size: 2742 4084 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4557 5515 442 Size: 416 53 83 399 24 3140 958 1753 Fragments arranged by size: 3140 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6826 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2990 3508 2990 Size: 518 6308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2452 3959 5486 1239 Size: 980 233 1507 1527 2579 Fragments arranged by size: 2579 1527 1507 980 233 Bpu10I CC'TnA_GC Cuts at: 3524 3524 Size: 6826 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3752 3791 4951 5816 6114 3752 Size: 39 1160 865 298 4464 Fragments arranged by size: 4464 1160 865 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2576 3338 5489 882 Size: 617 1077 762 2151 2219 Fragments arranged by size: 2219 2151 1077 762 617 BsaAI yAC'GTr Cuts at: 493 1204 3217 4617 493 Size: 711 2013 1400 2702 Fragments arranged by size: 2702 2013 1400 711 BsaBI GATnn'nnATC Cuts at: 6820 6820 Size: 6826 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 2508 2544 2591 Size: 53 83 186 936 771 204 36 47 Cuts at: 2591 3016 3200 3622 4065 4633 5015 5650 275 Size: 425 184 422 443 568 382 635 1451 Fragments arranged by size: 1451 936 771 635 568 443 425 422 382 204 186 184 83 53 47 36 BsaWI w'CCGG_w Cuts at: 2080 3481 5259 5657 5931 6078 6489 2080 Size: 1401 1778 398 274 147 411 2417 Fragments arranged by size: 2417 1778 1401 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2365 2395 1178 Size: 30 165 30 962 30 5609 Fragments arranged by size: 5609 962 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 2420 2827 2960 1186 Size: 1147 87 407 133 5052 Fragments arranged by size: 5052 1147 407 133 87 BseYI C'CCAG_C Cuts at: 2101 2551 2992 3110 3538 3842 6029 6525 2101 Size: 450 441 118 428 304 2187 496 2402 Fragments arranged by size: 2402 2187 496 450 441 428 304 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 3379 3854 1163 Size: 573 765 878 475 4135 Fragments arranged by size: 4135 878 765 573 475 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2064 2922 3519 3562 3651 4276 Size: 275 186 63 858 597 43 89 625 Cuts at: 4276 5037 6065 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 858 761 625 597 275 186 89 63 43 BsiHKAI G_wGCw'C Cuts at: 4887 4972 6043 4887 Size: 85 1071 5670 Fragments arranged by size: 5670 1071 85 BsmI GAATG_Cn' Cuts at: 4337 4430 4337 Size: 93 6733 BsmBI CGTCTCn'nnnn_ Cuts at: 2295 2295 Size: 6826 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2192 2648 2779 2814 3160 3768 Size: 399 343 59 456 131 35 346 608 Cuts at: 3768 4014 4145 4887 4972 6043 1391 Size: 246 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 608 456 399 346 343 246 131 131 85 59 35 BspEI T'CCGG_A Cuts at: 3481 6489 3481 Size: 3008 3818 BspHI T'CATG_A Cuts at: 4715 6445 4715 Size: 1730 5096 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2478 3375 3692 4181 844 Size: 1634 897 317 489 3489 Fragments arranged by size: 3489 1634 897 489 317 BsrBI CCG'CTC Cuts at: 1394 1823 2790 2924 3027 3448 4713 1394 Size: 429 967 134 103 421 1265 3507 Fragments arranged by size: 3507 1265 967 429 421 134 103 BsrDI GCAATG_nn' Cuts at: 65 1248 2294 5321 5495 65 Size: 1183 1046 3027 174 1396 Fragments arranged by size: 3027 1396 1183 1046 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2063 2080 2897 2969 3382 3424 3646 Size: 110 253 17 817 72 413 42 222 Cuts at: 3646 3716 5470 5657 1700 Size: 70 1754 187 2869 Fragments arranged by size: 2869 1754 817 413 253 222 187 110 72 70 42 17 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6826 BssHII G'CGCG_C Cuts at: 1925 3223 3306 1925 Size: 1298 83 5445 Fragments arranged by size: 5445 1298 83 BssSI C'ACGA_G Cuts at: 4886 5898 4886 Size: 1012 5814 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6826 BstBI TT'CG_AA Cuts at: 5630 5630 Size: 6826 BstEII G'GTnAC_C Cuts at: 1184 3056 1184 Size: 1872 4954 BstXI CCAn_nnnn'nTGG Cuts at: 2736 2736 Size: 6826 BstYI r'GATC_y Cuts at: 1972 2280 3771 4036 4156 4523 4908 4925 6377 Size: 308 1491 265 120 367 385 17 1452 Cuts at: 6377 6463 6475 6821 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1491 1452 385 367 346 308 265 120 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6274 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6087 Fragments arranged by size: 6087 683 56 BtsI GCAGTG_nn' Cuts at: 2479 4342 5136 5156 2479 Size: 1863 794 20 4149 Fragments arranged by size: 4149 1863 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6826 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6791 DraI TTT'AAA Cuts at: 4130 4130 Size: 6826 DraIII CAC_nnn'GTG Cuts at: 2485 2485 Size: 6826 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4064 5833 809 Size: 507 2748 1769 1802 Fragments arranged by size: 2748 1802 1769 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2712 2919 Size: 54 1425 50 275 18 231 651 207 Cuts at: 2919 3426 3516 3549 3648 3718 3967 4273 5162 Size: 507 90 33 99 70 249 306 889 Cuts at: 5162 6791 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 651 507 306 275 249 231 207 99 90 70 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2061 2919 3516 3648 4273 1537 Size: 275 249 858 597 132 625 4090 Fragments arranged by size: 4090 858 625 597 275 249 132 EarI CTCTTCn'nnn_ Cuts at: 1353 2704 3189 4756 1353 Size: 1351 485 1567 3423 Fragments arranged by size: 3423 1567 1351 485 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3193 5413 5787 5933 387 Size: 1064 138 1604 2220 374 146 1280 Fragments arranged by size: 2220 1604 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2452 3484 3494 3959 4889 5486 Size: 393 587 233 1032 10 465 930 597 Cuts at: 5486 6273 6541 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1032 930 787 597 587 465 393 268 233 10 EcoO109I rG'GnC_Cy Cuts at: 3115 3156 3157 3934 3115 Size: 41 1 777 6007 Fragments arranged by size: 6007 777 41 1 EcoRI G'AATT_C Cuts at: 4135 4513 4135 Size: 378 6448 FspI TGC'GCA Cuts at: 2153 3267 5332 2153 Size: 1114 2065 3647 Fragments arranged by size: 3647 2065 1114 FspAI rTGC'GCAy Cuts at: 2153 2153 Size: 6826 HaeII r_GCGC'y Cuts at: 1678 2027 2413 2511 2547 2594 3019 3304 5973 Size: 349 386 98 36 47 425 285 2669 Cuts at: 5973 1678 Size: 2531 Fragments arranged by size: 2669 2531 425 386 349 285 98 47 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2265 2297 2371 2403 3527 Size: 32 135 32 693 32 74 32 1124 Cuts at: 3527 3559 5467 5499 5541 5573 1373 Size: 32 1908 32 42 32 2626 Fragments arranged by size: 2626 1908 1124 693 135 74 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4170 4416 669 Size: 988 281 2232 246 3079 Fragments arranged by size: 3079 2232 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6826 HpaI GTT'AAC Cuts at: 4416 4416 Size: 6826 KasI G'GCGC_C Cuts at: 2507 2543 2590 3015 2507 Size: 36 47 425 6318 Fragments arranged by size: 6318 425 47 36 KpnI G_GTAC'C Cuts at: 2094 4151 2094 Size: 2057 4769 MfeI C'AATT_G Cuts at: 4425 4425 Size: 6826 MluI A'CGCG_T Cuts at: 6702 6702 Size: 6826 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2408 5940 6124 1305 Size: 1103 3532 184 2007 Fragments arranged by size: 3532 2007 1103 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3551 3969 10 Size: 54 1425 343 1719 418 2867 Fragments arranged by size: 2867 1719 1425 418 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 3808 4784 5143 5302 518 Size: 648 870 1772 976 359 159 2042 Fragments arranged by size: 2042 1772 976 870 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2493 2555 2767 3271 3504 3515 3589 3687 Size: 887 62 212 504 233 11 74 98 Cuts at: 3687 3696 3897 4242 4919 6067 6312 1606 Size: 9 201 345 677 1148 245 2120 Fragments arranged by size: 2120 1148 887 677 504 345 245 233 212 201 98 74 62 11 9 NaeI GCC'GGC Cuts at: 2065 2899 2971 3384 2065 Size: 834 72 413 5507 Fragments arranged by size: 5507 834 413 72 NarI GG'CG_CC Cuts at: 2508 2544 2591 3016 2508 Size: 36 47 425 6318 Fragments arranged by size: 6318 425 47 36 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6274 NdeI CA'TA_TG Cuts at: 387 387 Size: 6826 NgoMIV G'CCGG_C Cuts at: 2063 2897 2969 3382 2063 Size: 834 72 413 5507 Fragments arranged by size: 5507 834 413 72 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6826 NotI GC'GGCC_GC Cuts at: 2919 3516 4273 2919 Size: 597 757 5472 Fragments arranged by size: 5472 757 597 NspI r_CATG'y Cuts at: 1132 1229 3781 3950 4184 5729 1132 Size: 97 2552 169 234 1545 2229 Fragments arranged by size: 2552 2229 1545 234 169 97 PasI CC'CwG_GG Cuts at: 1348 2641 1348 Size: 1293 5533 PciI A'CATG_T Cuts at: 3946 5725 3946 Size: 1779 5047 PflMI CCAn_nnn'nTGG Cuts at: 1108 2641 1108 Size: 1533 5293 PmeI GTTT'AAAC Cuts at: 4130 4130 Size: 6826 PmlI CAC'GTG Cuts at: 3217 3217 Size: 6826 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4895 4927 6408 6440 4895 Size: 32 1481 32 5281 Fragments arranged by size: 5281 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3115 3934 3115 Size: 819 6007 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6826 PsiI TTA'TAA Cuts at: 4396 4396 Size: 6826 PspOMI G'GGCC_C Cuts at: 2644 3156 2644 Size: 512 6314 PspXI vC'TCGA_Gb Cuts at: 3296 3296 Size: 6826 PstI C_TGCA'G Cuts at: 830 3514 4178 830 Size: 2684 664 3478 Fragments arranged by size: 3478 2684 664 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6826 PvuII CAG'CTG Cuts at: 2767 3271 3589 2767 Size: 504 318 6004 Fragments arranged by size: 6004 504 318 RsrII CG'GwC_CG Cuts at: 2078 2078 Size: 6826 SalI G'TCGA_C Cuts at: 1936 4168 1936 Size: 2232 4594 SanDI GG'GwC_CC Cuts at: 3115 3115 Size: 6826 SbfI CC_TGCA'GG Cuts at: 4178 4178 Size: 6826 ScaI AGT'ACT Cuts at: 5074 6786 5074 Size: 1712 5114 SfcI C'TryA_G Cuts at: 826 1045 3510 4174 5309 5990 6181 826 Size: 219 2465 664 1135 681 191 1471 Fragments arranged by size: 2465 1471 1135 681 664 219 191 SfoI GGC'GCC Cuts at: 2509 2545 2592 3017 2509 Size: 36 47 425 6318 Fragments arranged by size: 6318 425 47 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6826 SmaI CCC'GGG Cuts at: 1872 2134 3022 3327 4002 4153 1872 Size: 262 888 305 675 151 4545 Fragments arranged by size: 4545 888 675 305 262 151 SmlI C'TyrA_G Cuts at: 820 1017 1943 2459 3296 3767 3806 4930 5831 Size: 197 926 516 837 471 39 1124 901 Cuts at: 5831 6093 820 Size: 262 1553 Fragments arranged by size: 1553 1124 926 901 837 516 471 262 197 39 SnaBI TAC'GTA Cuts at: 493 4617 493 Size: 4124 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6826 SphI G_CATG'C Cuts at: 1132 4184 1132 Size: 3052 3774 SrfI GCCC'GGGC Cuts at: 2134 2134 Size: 6826 SspI AAT'ATT Cuts at: 5 52 4750 5 Size: 47 4698 2081 Fragments arranged by size: 4698 2081 47 StuI AGG'CCT Cuts at: 1517 2663 2696 1517 Size: 1146 33 5647 Fragments arranged by size: 5647 1146 33 StyI C'CwwG_G Cuts at: 513 1065 2346 2539 4265 513 Size: 552 1281 193 1726 3074 Fragments arranged by size: 3074 1726 1281 552 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2558 3576 4853 4870 5023 5208 5653 Size: 1255 978 1018 1277 17 153 185 445 Cuts at: 5653 325 Size: 1498 Fragments arranged by size: 1498 1277 1255 1018 978 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3747 5072 6784 Size: 274 80 33 51 1443 1770 1325 1712 Cuts at: 6784 96 Size: 138 Fragments arranged by size: 1770 1712 1443 1325 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2365 2397 6786 6818 2365 Size: 32 4389 32 2373 Fragments arranged by size: 4389 2373 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6826 XbaI T'CTAG_A Cuts at: 4162 4162 Size: 6826 XhoI C'TCGA_G Cuts at: 1943 3296 1943 Size: 1353 5473 XmaI C'CCGG_G Cuts at: 1870 2132 3020 3325 4000 4151 1870 Size: 262 888 305 675 151 4545 Fragments arranged by size: 4545 888 675 305 262 151 XmnI GAAnn'nnTTC Cuts at: 4955 4955 Size: 6826 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2305 4066 4634 276 Size: 53 83 186 936 771 1761 568 2468 Fragments arranged by size: 2468 1761 936 771 568 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PfoI PsrI SacI SacII SapI SexAI SfiI SwaI XcmI