(Linear) (Six-base) MAPSORT of: KIEE5727.seq Check: 3256 from: 1 to: 2121 KIEE5727.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 473 1370 2121 Size: 473 897 751 Fragments arranged by size: 897 751 473 AatII G_ACGT'C Cuts at: 0 302 2063 2121 Size: 302 1761 58 Fragments arranged by size: 1761 302 58 AccI GT'mk_AC Cuts at: 0 1391 2121 Size: 1391 730 Acc65I G'GTAC_C Cuts at: 0 85 2121 Size: 85 2036 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1479 1489 2121 Size: 1479 10 632 Fragments arranged by size: 1479 632 10 AfeI AGC'GCT Cuts at: 0 406 1297 2121 Size: 406 891 824 Fragments arranged by size: 891 824 406 AflII C'TTAA_G Cuts at: 0 454 2121 Size: 454 1667 AflIII A'CryG_T Cuts at: 0 1941 2121 Size: 1941 180 AgeI A'CCGG_T Cuts at: 0 75 2121 Size: 75 2046 AleI CACnn'nnGTG Cuts at: 0 31 1803 2121 Size: 31 1772 318 Fragments arranged by size: 1772 318 31 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1378 2121 Size: 1378 743 AlwNI CAG_nnn'CTG Cuts at: 0 243 553 1584 1971 2121 Size: 243 310 1031 387 150 Fragments arranged by size: 1031 387 310 243 150 ApaI G_GGCC'C Cuts at: 0 643 1155 2121 Size: 643 512 966 Fragments arranged by size: 966 643 512 AvaI C'yCGr_G Cuts at: 0 127 794 1015 1291 1320 1626 1995 2089 Size: 127 667 221 276 29 306 369 94 Cuts at: 2089 2121 Size: 32 Fragments arranged by size: 667 369 306 276 221 127 94 32 29 BamHI G'GATC_C Cuts at: 0 2031 2121 Size: 2031 90 BanI G'GyrC_C Cuts at: 0 78 85 502 538 585 1010 1663 1999 Size: 78 7 417 36 47 425 653 336 Cuts at: 1999 2121 Size: 122 Fragments arranged by size: 653 425 417 336 122 78 47 36 7 BanII G_rGCy'C Cuts at: 0 128 187 643 1155 1763 2009 2121 Size: 128 59 456 512 608 246 112 Fragments arranged by size: 608 512 456 246 128 112 59 BbeI G_GCGC'C Cuts at: 0 506 542 589 1014 2121 Size: 506 36 47 425 1107 Fragments arranged by size: 1107 506 425 47 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1579 1613 2121 Size: 1579 34 508 Fragments arranged by size: 1579 508 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1484 2121 Size: 1484 637 BglI GCCn_nnn'nGGC Cuts at: 0 1651 1961 2121 Size: 1651 310 160 Fragments arranged by size: 1651 310 160 BglII A'GATC_T Cuts at: 0 275 2121 Size: 275 1846 BlpI GC'TnA_GC Cuts at: 0 876 2121 Size: 876 1245 Bme1580I G_kGCm'C Cuts at: 0 643 774 809 1155 2121 Size: 643 131 35 346 966 Fragments arranged by size: 966 643 346 131 35 BmgBI CAC'GTC Cuts at: 0 2055 2121 Size: 2055 66 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 985 1503 2121 Size: 985 518 618 Fragments arranged by size: 985 618 518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 447 1954 2121 Size: 214 233 1507 167 Fragments arranged by size: 1507 233 214 167 Bpu10I CC'TnA_GC Cuts at: 0 1519 2121 Size: 1519 602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1747 1786 2121 Size: 1747 39 335 Fragments arranged by size: 1747 335 39 BsaI GGTCTCn'nnnn_ Cuts at: 0 571 1333 2121 Size: 571 762 788 Fragments arranged by size: 788 762 571 BsaAI yAC'GTr Cuts at: 0 1212 2121 Size: 1212 909 BsaHI Gr'CG_yC Cuts at: 0 299 503 539 586 1011 1195 1617 2060 Size: 299 204 36 47 425 184 422 443 Cuts at: 2060 2121 Size: 61 Fragments arranged by size: 443 425 422 299 204 184 61 47 36 BsaWI w'CCGG_w Cuts at: 0 75 1476 2121 Size: 75 1401 645 Fragments arranged by size: 1401 645 75 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 360 390 2121 Size: 360 30 1731 Fragments arranged by size: 1731 360 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 328 415 822 955 2121 Size: 328 87 407 133 1166 Fragments arranged by size: 1166 407 328 133 87 BseYI C'CCAG_C Cuts at: 0 96 546 987 1105 1533 1837 2121 Size: 96 450 441 118 428 304 284 Fragments arranged by size: 450 441 428 304 284 118 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 496 1374 1849 2121 Size: 496 878 475 272 Fragments arranged by size: 878 496 475 272 BsiEI CG_ry'CG Cuts at: 0 59 917 1514 1557 1646 2121 Size: 59 858 597 43 89 475 Fragments arranged by size: 858 597 475 89 59 43 BsmBI CGTCTCn'nnnn_ Cuts at: 0 290 2121 Size: 290 1831 Bsp1286I G_dGCh'C Cuts at: 0 128 187 643 774 809 1155 1763 2009 Size: 128 59 456 131 35 346 608 246 Cuts at: 2009 2121 Size: 112 Fragments arranged by size: 608 456 346 246 131 128 112 59 35 BspEI T'CCGG_A Cuts at: 0 1476 2121 Size: 1476 645 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 473 1370 1687 2121 Size: 473 897 317 434 Fragments arranged by size: 897 473 434 317 BsrBI CCG'CTC Cuts at: 0 785 919 1022 1443 2121 Size: 785 134 103 421 678 Fragments arranged by size: 785 678 421 134 103 BsrDI GCAATG_nn' Cuts at: 0 289 2121 Size: 289 1832 BsrFI r'CCGG_y Cuts at: 0 58 75 892 964 1377 1419 1641 1711 Size: 58 17 817 72 413 42 222 70 Cuts at: 1711 2121 Size: 410 Fragments arranged by size: 817 413 410 222 72 70 58 42 17 BssHII G'CGCG_C Cuts at: 0 1218 1301 2121 Size: 1218 83 820 Fragments arranged by size: 1218 820 83 BstEII G'GTnAC_C Cuts at: 0 1051 2121 Size: 1051 1070 BstXI CCAn_nnnn'nTGG Cuts at: 0 731 2121 Size: 731 1390 BstYI r'GATC_y Cuts at: 0 275 1766 2031 2121 Size: 275 1491 265 90 Fragments arranged by size: 1491 275 265 90 BtsI GCAGTG_nn' Cuts at: 0 474 2121 Size: 474 1647 DraIII CAC_nnn'GTG Cuts at: 0 480 2121 Size: 480 1641 DrdI GACnn_nn'nnGTC Cuts at: 0 2059 2121 Size: 2059 62 EaeI y'GGCC_r Cuts at: 0 56 707 914 1421 1511 1544 1643 1713 Size: 56 651 207 507 90 33 99 70 Cuts at: 1713 1962 2121 Size: 249 159 Fragments arranged by size: 651 507 249 207 159 99 90 70 56 33 EagI C'GGCC_G Cuts at: 0 56 914 1511 1643 2121 Size: 56 858 597 132 478 Fragments arranged by size: 858 597 478 132 56 EarI CTCTTCn'nnn_ Cuts at: 0 699 1184 2121 Size: 699 485 937 Fragments arranged by size: 937 699 485 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1188 2121 Size: 1188 933 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 447 1479 1489 1954 2121 Size: 214 233 1032 10 465 167 Fragments arranged by size: 1032 465 233 214 167 10 EcoO109I rG'GnC_Cy Cuts at: 0 1110 1151 1152 1929 2121 Size: 1110 41 1 777 192 Fragments arranged by size: 1110 777 192 41 1 FspI TGC'GCA Cuts at: 0 148 1262 2121 Size: 148 1114 859 Fragments arranged by size: 1114 859 148 FspAI rTGC'GCAy Cuts at: 0 148 2121 Size: 148 1973 HaeII r_GCGC'y Cuts at: 0 22 408 506 542 589 1014 1299 2121 Size: 22 386 98 36 47 425 285 822 Fragments arranged by size: 822 425 386 285 98 47 36 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 260 292 366 398 1522 1554 2121 Size: 260 32 74 32 1124 32 567 Fragments arranged by size: 1124 567 260 74 32 32 32 KasI G'GCGC_C Cuts at: 0 502 538 585 1010 2121 Size: 502 36 47 425 1111 Fragments arranged by size: 1111 502 425 47 36 KpnI G_GTAC'C Cuts at: 0 89 2121 Size: 89 2032 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 403 2121 Size: 403 1718 MscI TGG'CCA Cuts at: 0 1546 1964 2121 Size: 1546 418 157 Fragments arranged by size: 1546 418 157 MslI CAynn'nnrTG Cuts at: 0 31 1803 2121 Size: 31 1772 318 Fragments arranged by size: 1772 318 31 MspA1I CmG'CkG Cuts at: 0 488 550 762 1266 1499 1510 1584 1682 Size: 488 62 212 504 233 11 74 98 Cuts at: 1682 1691 1892 2121 Size: 9 201 229 Fragments arranged by size: 504 488 233 229 212 201 98 74 62 11 9 NaeI GCC'GGC Cuts at: 0 60 894 966 1379 2121 Size: 60 834 72 413 742 Fragments arranged by size: 834 742 413 72 60 NarI GG'CG_CC Cuts at: 0 503 539 586 1011 2121 Size: 503 36 47 425 1110 Fragments arranged by size: 1110 503 425 47 36 NgoMIV G'CCGG_C Cuts at: 0 58 892 964 1377 2121 Size: 58 834 72 413 744 Fragments arranged by size: 834 744 413 72 58 NotI GC'GGCC_GC Cuts at: 0 914 1511 2121 Size: 914 597 610 Fragments arranged by size: 914 610 597 NspI r_CATG'y Cuts at: 0 1776 1945 2121 Size: 1776 169 176 Fragments arranged by size: 1776 176 169 PasI CC'CwG_GG Cuts at: 0 636 2121 Size: 636 1485 PciI A'CATG_T Cuts at: 0 1941 2121 Size: 1941 180 PflMI CCAn_nnn'nTGG Cuts at: 0 636 2121 Size: 636 1485 PmlI CAC'GTG Cuts at: 0 1212 2121 Size: 1212 909 PpuMI rG'GwC_Cy Cuts at: 0 1110 1929 2121 Size: 1110 819 192 Fragments arranged by size: 1110 819 192 PspOMI G'GGCC_C Cuts at: 0 639 1151 2121 Size: 639 512 970 Fragments arranged by size: 970 639 512 PspXI vC'TCGA_Gb Cuts at: 0 1291 2121 Size: 1291 830 PstI C_TGCA'G Cuts at: 0 1509 2121 Size: 1509 612 PvuII CAG'CTG Cuts at: 0 762 1266 1584 2121 Size: 762 504 318 537 Fragments arranged by size: 762 537 504 318 RsrII CG'GwC_CG Cuts at: 0 73 2121 Size: 73 2048 SanDI GG'GwC_CC Cuts at: 0 1110 2121 Size: 1110 1011 SfcI C'TryA_G Cuts at: 0 1505 2121 Size: 1505 616 SfoI GGC'GCC Cuts at: 0 504 540 587 1012 2121 Size: 504 36 47 425 1109 Fragments arranged by size: 1109 504 425 47 36 SmaI CCC'GGG Cuts at: 0 129 1017 1322 1997 2121 Size: 129 888 305 675 124 Fragments arranged by size: 888 675 305 129 124 SmlI C'TyrA_G Cuts at: 0 454 1291 1762 1801 2121 Size: 454 837 471 39 320 Fragments arranged by size: 837 471 454 320 39 SrfI GCCC'GGGC Cuts at: 0 129 2121 Size: 129 1992 StuI AGG'CCT Cuts at: 0 658 691 2121 Size: 658 33 1430 Fragments arranged by size: 1430 658 33 StyI C'CwwG_G Cuts at: 0 341 534 2121 Size: 341 193 1587 Fragments arranged by size: 1587 341 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 553 1571 2121 Size: 553 1018 550 Fragments arranged by size: 1018 553 550 TatI w'GTAC_w Cuts at: 0 1742 2121 Size: 1742 379 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 360 392 2121 Size: 360 32 1729 Fragments arranged by size: 1729 360 32 XhoI C'TCGA_G Cuts at: 0 1291 2121 Size: 1291 830 XmaI C'CCGG_G Cuts at: 0 127 1015 1320 1995 2121 Size: 127 888 305 675 126 Fragments arranged by size: 888 675 305 127 126 ZraI GAC'GTC Cuts at: 0 300 2061 2121 Size: 300 1761 60 Fragments arranged by size: 1761 300 60 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflII AflIII AgeI AleI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstEII BstXI BstYI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I FspI FspAI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI PciI PflMI PmlI PpuMI PspOMI PspXI PstI PvuII RsrII SanDI SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XhoI XmaI ZraI Enzymes that do not cut: AclI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BclI BfrBI BmrI BmtI BsaBI BsiHKAI BsiWI BsmI BspHI BsrGI BssSI BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI EcoICRI EcoNI EcoRI EcoRV FalI FseI HincII HindIII HpaI MfeI MluI NcoI NdeI NheI NruI NsiI PacI PfoI PmeI PpiI PshAI PsiI PsrI PvuI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XcmI XmnI