################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI Alw44I AlwNI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsmFI BsmI BsoBI Bsp1407I BspDI BspHI BsrBI BsrDI BsrFI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI MaeI MamI MfeI MluNI MscI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SpeI SphI SspBI SspI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 145 GTAGAC GT^[AC][GT]AC AccIII 1127 TCCGGA T^CCGGA Aor51HI 494 AGCGCT AGC^GCT BbeI 3 GGCGCC GGCGC^C BcgI 1227 GCAAAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 106 CCCGG CC^[GC]GG BglI 1213 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 142 TACGTA [CT]AC^GT[GA] BsaWI 1127 TCCGGA [AT]^CCGG[AT] BseAI 1127 TCCGGA T^CCGGA BsgI 844 GTGCAG GTGCAG(16/14) BsiEI 108 CGGCCG CG[GA][CT]^CG BsiWI 413 CGTACG C^GTACG BsmBI 592 GAGACG CGTCTC(1/5) BspEI 1127 TCCGGA T^CCGGA BspLU11I 1166 ACATGT A^CATGT BsrI 958 ACTGG ACTGG(1/-1) BssHII 603 GCGCGC G^CGCGC BssSI 850 CACGAG CACGAG(-5/-1) EaeI 108 CGGCCG [CT]^GGCC[GA] EagI 108 CGGCCG C^GGCCG EclXI 108 CGGCCG C^GGCCG Eco47III 494 AGCGCT AGC^GCT Eco52I 108 CGGCCG C^GGCCG EcoNI 438 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HindIII 1080 AAGCTT A^AGCTT KasI 3 GGCGCC G^GCGCC MluI 410 ACGCGT A^CGCGT MroI 1127 TCCGGA T^CCGGA MseI 1313 TTAA T^TAA NarI 3 GGCGCC GG^CGCC NciI 106 CCCGG CC^[GC]GG PleI 1131 GAGTC GAGTC(4/5) SacI 996 GAGCTC GAGCT^C SbfI 766 CCTGCAGG CCTGCA^GG SfaNI 920 GCATC GCATC(5/9) SfoI 3 GGCGCC GGC^GCC SmlI 75 CTTGAG C^T[CT][GA]AG SnaBI 142 TACGTA TAC^GTA Sse8387I 766 CCTGCAGG CCTGCA^GG StuI 763 AGGCCT AGG^CCT TaqI 170 TCGA T^CGA TfiI 186 GATTC G^A[AT]TC Tru9I 1313 TTAA T^TAA Tsp509I 158 AATT ^AATT TthHB8I 170 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 610 GATCC GGATC(4/5) 1172 GGATC AocI 294 CCTGAGG CC^T[ACGT]AGG 663 CCTGAGG AspHI 996 GAGCTC G[AT]GC[AT]^C 1099 GTGCTC BsaI 757 GAGACC GGTCTC(1/5) 335 GGTCTC BsiHKAI 996 GAGCTC G[AT]GC[AT]^C 1099 GTGCTC BspMI 302 ACCTGC ACCTGC(4/8) 449 ACCTGC BstAPI 156 GCAATTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 622 GCACACGTTGC BstYI 609 AGATCC [GA]^GATC[CT] 1172 GGATCT Bsu36I 294 CCTGAGG CC^T[ACGT]AGG 663 CCTGAGG EarI 1102 CTCTTC CTCTTC(1/4) 993 GAAGAG Eco81I 294 CCTGAGG CC^T[ACGT]AGG 663 CCTGAGG HaeII 3 GGCGCC [GA]GCGC^[CT] 494 AGCGCT HinfI 186 GATTC G^A[ACGT]TC 1131 GAGTC Ksp632I 1102 CTCTTC CTCTTC(1/4) 993 GAAGAG MaeII 143 ACGT A^CGT 626 ACGT MflI 609 AGATCC [GA]^GATC[CT] 1172 GGATCT MslI 90 CACCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 129 CATCGCCGTG NspI 835 ACATGC [GA]CATG^[CT] 1166 ACATGT PpuMI 446 AGGACCT [GA]G^G[AT]CC[CT] 532 AGGACCT PstI 767 CTGCAG CTGCA^G 1031 CTGCAG SapI 993 GAAGAGC GCTCTTC(1/4) 1101 GCTCTTC XhoII 609 AGATCC [GA]^GATC[CT] 1172 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI ApaI AvaII BanI BanII BbvI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KspI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV RsaI SacII Sau3AI Sau96I ScrFI SfcI StyI TseI Tsp45I TspRI XcmI