(Circular) (Six-base) MAPSORT of: pFN21AE1907.seq Check: 8851 from: 1 to: 6031 pFN21AE1907 6031 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 278 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2151 3374 1937 Size: 214 1223 4594 Fragments arranged by size: 4594 1223 214 Acc65I G'GTAC_C Cuts at: 3352 3352 Size: 6031 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4094 5478 5746 1632 Size: 2462 1384 268 1917 Fragments arranged by size: 2462 1917 1384 268 AfeI AGC'GCT Cuts at: 2501 2501 Size: 6031 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 2415 2629 3171 4930 5907 1203 Size: 1212 214 542 1759 977 1327 Fragments arranged by size: 1759 1327 1212 977 542 214 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 2099 1166 Size: 933 5098 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6031 AlwNI CAG_nnn'CTG Cuts at: 3774 5346 3774 Size: 1572 4459 ApaI G_GGCC'C Cuts at: 2044 2382 2683 2044 Size: 338 301 5392 Fragments arranged by size: 5392 338 301 ApaLI G'TGCA_C Cuts at: 4088 5244 4088 Size: 1156 4875 ApoI r'AATT_y Cuts at: 1493 3340 3566 3718 5827 1493 Size: 1847 226 152 2109 1697 Fragments arranged by size: 2109 1847 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 3356 1870 Size: 73 1413 4545 Fragments arranged by size: 4545 1413 73 BamHI G'GATC_C Cuts at: 3361 3728 3361 Size: 367 5664 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2812 3291 3352 4807 Size: 325 217 627 221 804 479 61 1455 Cuts at: 4807 618 Size: 1842 Fragments arranged by size: 1842 1455 804 627 479 325 221 217 61 BanII G_rGCy'C Cuts at: 1391 2044 2072 2127 2382 2683 3005 3350 1391 Size: 653 28 55 255 301 322 345 4072 Fragments arranged by size: 4072 653 345 322 301 255 55 28 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 6031 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5060 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3221 3255 4222 4256 1903 Size: 34 1284 34 967 34 3678 Fragments arranged by size: 3678 1284 967 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3923 5133 3923 Size: 1210 4821 BclI T'GATC_A Cuts at: 1548 1548 Size: 6031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3224 4642 136 Size: 107 122 71 631 605 1552 1418 1525 Fragments arranged by size: 1552 1525 1418 631 605 122 107 71 BglII A'GATC_T Cuts at: 6026 6026 Size: 6031 BlpI GC'TnA_GC Cuts at: 3448 3448 Size: 6031 Bme1580I G_kGCm'C Cuts at: 1790 2044 2382 2683 4092 5248 1790 Size: 254 338 301 1409 1156 2573 Fragments arranged by size: 2573 1409 1156 338 301 254 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6031 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3762 4720 442 Size: 416 53 83 399 24 2345 958 1753 Fragments arranged by size: 2345 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3045 3045 Size: 6031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2567 2582 2841 2985 4691 1239 Size: 1097 231 15 259 144 1706 2579 Fragments arranged by size: 2579 1706 1097 259 231 144 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2101 4156 5021 5319 2101 Size: 2055 865 298 2813 Fragments arranged by size: 2813 2055 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2346 2756 4694 882 Size: 617 847 410 1938 2219 Fragments arranged by size: 2219 1938 847 617 410 BsaAI yAC'GTr Cuts at: 493 1204 2149 3822 493 Size: 711 945 1673 2702 Fragments arranged by size: 2702 1673 945 711 BsaBI GATnn'nnATC Cuts at: 6025 6025 Size: 6031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2184 2389 3838 Size: 53 83 186 936 476 175 205 1449 Cuts at: 3838 4220 4855 275 Size: 382 635 1451 Fragments arranged by size: 1451 1449 936 635 476 382 205 186 175 83 53 BsaWI w'CCGG_w Cuts at: 3132 4464 4862 5136 5283 5694 3132 Size: 1332 398 274 147 411 3469 Fragments arranged by size: 3469 1332 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2379 2409 2444 2474 2540 Size: 30 165 30 976 30 35 30 66 Cuts at: 2540 2570 2943 2958 2973 2988 3127 3157 1178 Size: 30 373 15 15 15 139 30 4052 Fragments arranged by size: 4052 976 373 165 139 66 35 30 30 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2384 2543 1186 Size: 826 372 159 4674 Fragments arranged by size: 4674 826 372 159 BseYI C'CCAG_C Cuts at: 2336 2496 2676 2868 2901 5234 5730 2336 Size: 160 180 192 33 2333 496 2637 Fragments arranged by size: 2637 2333 496 192 180 160 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2870 1163 Size: 573 1134 4324 Fragments arranged by size: 4324 1134 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2116 3481 4242 5270 1540 Size: 275 186 115 1365 761 1028 2301 Fragments arranged by size: 2301 1365 1028 761 275 186 115 BsiHKAI G_wGCw'C Cuts at: 3005 3108 4092 4177 5248 3005 Size: 103 984 85 1071 3788 Fragments arranged by size: 3788 1071 984 103 85 BsiWI C'GTAC_G Cuts at: 2418 2418 Size: 6031 BsmI GAATG_Cn' Cuts at: 3542 3635 3542 Size: 93 5938 BsmBI CGTCTCn'nnnn_ Cuts at: 2591 2591 Size: 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2072 2127 2382 2683 3005 3108 Size: 399 254 28 55 255 301 322 103 Cuts at: 3108 3350 4092 4177 5248 1391 Size: 242 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 322 301 255 254 242 103 85 55 28 BspEI T'CCGG_A Cuts at: 3132 5694 3132 Size: 2562 3469 BspHI T'CATG_A Cuts at: 3920 5650 3920 Size: 1730 4301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2316 2463 3386 844 Size: 1472 147 923 3489 Fragments arranged by size: 3489 1472 923 147 BsrBI CCG'CTC Cuts at: 1394 1823 3918 1394 Size: 429 2095 3507 Fragments arranged by size: 3507 2095 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4526 4700 65 Size: 1183 3278 174 1396 Fragments arranged by size: 3278 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4675 4862 1700 Size: 110 2865 187 2869 Fragments arranged by size: 2869 2865 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6031 BssHII G'CGCG_C Cuts at: 1925 2608 1925 Size: 683 5348 BssSI C'ACGA_G Cuts at: 2855 4091 5103 2855 Size: 1236 1012 3783 Fragments arranged by size: 3783 1236 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2167 2633 794 Size: 1373 466 4192 Fragments arranged by size: 4192 1373 466 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstYI r'GATC_y Cuts at: 1972 2614 3177 3361 3728 4113 4130 5582 5668 Size: 642 563 184 367 385 17 1452 86 Cuts at: 5668 5680 6026 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 642 563 385 367 346 184 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2300 2669 2300 Size: 369 5662 BtgI C'CryG_G Cuts at: 513 1065 2004 2116 2139 2207 2216 2398 2469 Size: 552 939 112 23 68 9 182 71 Cuts at: 2469 2481 513 Size: 12 4063 Fragments arranged by size: 4063 939 552 182 112 71 68 23 12 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2119 487 Size: 683 56 893 4399 Fragments arranged by size: 4399 893 683 56 BtsI GCAGTG_nn' Cuts at: 3213 3547 4341 4361 3213 Size: 334 794 20 4883 Fragments arranged by size: 4883 794 334 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 3335 3335 Size: 6031 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5038 809 Size: 507 3722 1802 Fragments arranged by size: 3722 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2113 3478 4367 Size: 54 1425 50 275 18 283 1365 889 Cuts at: 4367 5996 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1365 889 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2113 3478 1537 Size: 275 301 1365 4090 Fragments arranged by size: 4090 1365 301 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2993 3113 3961 1353 Size: 1640 120 848 3423 Fragments arranged by size: 3423 1640 848 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2303 2489 4618 4992 5138 387 Size: 1064 138 714 186 2129 374 146 1280 Fragments arranged by size: 2129 1280 1064 714 374 186 146 138 EcoICRI GAG'CTC Cuts at: 3003 3003 Size: 6031 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2567 2582 2841 2985 4094 4691 Size: 393 704 231 15 259 144 1109 597 Cuts at: 4691 5478 5746 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1109 787 704 597 393 268 259 231 144 15 EcoNI CCTnn'n_nnAGG Cuts at: 2447 2447 Size: 6031 EcoO109I rG'GnC_Cy Cuts at: 2040 2041 2378 2452 2538 2680 2040 Size: 1 337 74 86 142 5391 Fragments arranged by size: 5391 337 142 86 74 1 EcoRI G'AATT_C Cuts at: 3340 3718 3340 Size: 378 5653 FspI TGC'GCA Cuts at: 4537 4537 Size: 6031 HaeII r_GCGC'y Cuts at: 1678 2012 2503 5178 1678 Size: 334 491 2675 2531 Fragments arranged by size: 2675 2531 491 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2324 2356 2379 2411 2423 Size: 32 135 32 752 32 23 32 12 Cuts at: 2423 2444 2455 2476 2943 2958 2975 2990 4672 Size: 21 11 21 467 15 17 15 1682 Cuts at: 4672 4704 4746 4778 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1682 752 467 135 42 32 32 32 32 32 32 23 21 21 17 15 15 12 11 HincII GTy'rAC Cuts at: 669 1657 1938 3375 3621 669 Size: 988 281 1437 246 3079 Fragments arranged by size: 3079 1437 988 281 246 HindIII A'AGCT_T Cuts at: 748 3085 748 Size: 2337 3694 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KasI G'GCGC_C Cuts at: 2008 2008 Size: 6031 KpnI G_GTAC'C Cuts at: 3356 3356 Size: 6031 MfeI C'AATT_G Cuts at: 3630 3630 Size: 6031 MluI A'CGCG_T Cuts at: 2415 5907 2415 Size: 3492 2539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5145 5329 1305 Size: 3840 184 2007 Fragments arranged by size: 3840 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4209 Fragments arranged by size: 4209 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2138 3989 4348 4507 518 Size: 648 933 39 1851 359 159 2042 Fragments arranged by size: 2042 1851 933 648 359 159 39 MspA1I CmG'CkG Cuts at: 1606 2099 2118 2209 2400 2471 2722 2804 3057 Size: 493 19 91 191 71 251 82 253 Cuts at: 3057 3447 4124 5272 5517 1606 Size: 390 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 493 390 253 251 245 191 91 82 71 19 NarI GG'CG_CC Cuts at: 2009 2009 Size: 6031 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4540 Fragments arranged by size: 4540 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6031 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 3478 3478 Size: 6031 NspI r_CATG'y Cuts at: 1132 1229 2844 3175 3389 4934 1132 Size: 97 1615 331 214 1545 2229 Fragments arranged by size: 2229 1615 1545 331 214 97 PasI CC'CwG_GG Cuts at: 1348 2683 1348 Size: 1335 4696 PciI A'CATG_T Cuts at: 3171 4930 3171 Size: 1759 4272 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6031 PfoI T'CCnGG_A Cuts at: 2462 2462 Size: 6031 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5613 5645 4100 Size: 32 1481 32 4486 Fragments arranged by size: 4486 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2538 2452 Size: 86 5945 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6031 PsiI TTA'TAA Cuts at: 3601 3601 Size: 6031 PspOMI G'GGCC_C Cuts at: 2040 2378 2679 2040 Size: 338 301 5392 Fragments arranged by size: 5392 338 301 PstI C_TGCA'G Cuts at: 830 2776 3040 3383 830 Size: 1946 264 343 3478 Fragments arranged by size: 3478 1946 343 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 SacI G_AGCT'C Cuts at: 3005 3005 Size: 6031 SacII CC_GC'GG Cuts at: 2119 2210 2401 2472 2119 Size: 91 191 71 5678 Fragments arranged by size: 5678 191 91 71 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SapI GCTCTTCn'nnn_ Cuts at: 2993 3113 2993 Size: 120 5911 SbfI CC_TGCA'GG Cuts at: 2776 3383 2776 Size: 607 5424 ScaI AGT'ACT Cuts at: 4279 5991 4279 Size: 1712 4319 SfcI C'TryA_G Cuts at: 826 1045 2603 2772 3036 3379 4514 5195 5386 Size: 219 1558 169 264 343 1135 681 191 Cuts at: 5386 826 Size: 1471 Fragments arranged by size: 1558 1471 1135 681 343 264 219 191 169 SfoI GGC'GCC Cuts at: 2010 2010 Size: 6031 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 3358 1872 Size: 1486 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2080 4135 5036 5298 820 Size: 197 926 137 2055 901 262 1553 Fragments arranged by size: 2055 1553 926 901 262 197 137 SnaBI TAC'GTA Cuts at: 493 2149 3822 493 Size: 1656 1673 2702 Fragments arranged by size: 2702 1673 1656 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 3389 1132 Size: 2257 3774 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 2770 1517 Size: 1253 4778 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 2947 3112 3470 513 Size: 552 939 264 679 165 358 3074 Fragments arranged by size: 3074 939 679 552 358 264 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2069 2209 2304 2510 3155 4058 4075 Size: 1255 489 140 95 206 645 903 17 Cuts at: 4075 4228 4413 4858 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 903 645 489 445 206 185 153 140 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4277 5989 96 Size: 274 80 33 51 1443 2300 1712 138 Fragments arranged by size: 2300 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2540 2572 3127 3151 3159 3183 5991 6023 2540 Size: 32 555 24 8 24 2808 32 2548 Fragments arranged by size: 2808 2548 555 32 32 24 24 8 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2333 2360 2917 2333 Size: 27 557 5447 Fragments arranged by size: 5447 557 27 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 3356 1870 Size: 1486 4545 XmnI GAAnn'nnTTC Cuts at: 4160 4160 Size: 6031 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 276 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SrfI SwaI