(Linear) (Six-base) MAPSORT of: KIEE1907.seq Check: 4020 from: 1 to: 1326 KIEE1907.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AccI GT'mk_AC Cuts at: 0 146 1326 Size: 146 1180 AfeI AGC'GCT Cuts at: 0 496 1326 Size: 496 830 AflIII A'CryG_T Cuts at: 0 410 624 1166 1326 Size: 410 214 542 160 Fragments arranged by size: 542 410 214 160 AleI CACnn'nnGTG Cuts at: 0 94 1326 Size: 94 1232 ApaI G_GGCC'C Cuts at: 0 39 377 678 1326 Size: 39 338 301 648 Fragments arranged by size: 648 338 301 39 BanI G'GyrC_C Cuts at: 0 3 807 1286 1326 Size: 3 804 479 40 Fragments arranged by size: 804 479 40 3 BanII G_rGCy'C Cuts at: 0 39 67 122 377 678 1000 1326 Size: 39 28 55 255 301 322 326 Fragments arranged by size: 326 322 301 255 55 39 28 BbeI G_GCGC'C Cuts at: 0 7 1326 Size: 7 1319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1216 1250 1326 Size: 1216 34 76 Fragments arranged by size: 1216 76 34 BglI GCCn_nnn'nGGC Cuts at: 0 1219 1326 Size: 1219 107 Bme1580I G_kGCm'C Cuts at: 0 39 377 678 1326 Size: 39 338 301 648 Fragments arranged by size: 648 338 301 39 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1040 1326 Size: 1040 286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 562 577 836 980 1326 Size: 331 231 15 259 144 346 Fragments arranged by size: 346 331 259 231 144 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 1326 Size: 96 1230 BsaI GGTCTCn'nnnn_ Cuts at: 0 341 751 1326 Size: 341 410 575 Fragments arranged by size: 575 410 341 BsaAI yAC'GTr Cuts at: 0 144 1326 Size: 144 1182 BsaHI Gr'CG_yC Cuts at: 0 4 179 384 1326 Size: 4 175 205 942 Fragments arranged by size: 942 205 175 4 BsaWI w'CCGG_w Cuts at: 0 1127 1326 Size: 1127 199 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 374 404 439 469 535 565 938 953 Size: 374 30 35 30 66 30 373 15 Cuts at: 953 968 983 1122 1152 1326 Size: 15 15 139 30 174 Fragments arranged by size: 374 373 174 139 66 35 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 7 379 538 1326 Size: 7 372 159 788 Fragments arranged by size: 788 372 159 7 BseYI C'CCAG_C Cuts at: 0 331 491 671 863 896 1326 Size: 331 160 180 192 33 430 Fragments arranged by size: 430 331 192 180 160 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 865 1326 Size: 865 461 BsiEI CG_ry'CG Cuts at: 0 111 1326 Size: 111 1215 BsiHKAI G_wGCw'C Cuts at: 0 1000 1103 1326 Size: 1000 103 223 Fragments arranged by size: 1000 223 103 BsiWI C'GTAC_G Cuts at: 0 413 1326 Size: 413 913 BsmBI CGTCTCn'nnnn_ Cuts at: 0 586 1326 Size: 586 740 Bsp1286I G_dGCh'C Cuts at: 0 39 67 122 377 678 1000 1103 1326 Size: 39 28 55 255 301 322 103 223 Fragments arranged by size: 322 301 255 223 103 55 39 28 BspEI T'CCGG_A Cuts at: 0 1127 1326 Size: 1127 199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 311 458 1326 Size: 311 147 868 Fragments arranged by size: 868 311 147 BssHII G'CGCG_C Cuts at: 0 603 1326 Size: 603 723 BssSI C'ACGA_G Cuts at: 0 850 1326 Size: 850 476 BstAPI GCAn_nnn'nTGC Cuts at: 0 162 628 1326 Size: 162 466 698 Fragments arranged by size: 698 466 162 BstYI r'GATC_y Cuts at: 0 609 1172 1326 Size: 609 563 154 Fragments arranged by size: 609 563 154 Bsu36I CC'TnA_GG Cuts at: 0 295 664 1326 Size: 295 369 662 Fragments arranged by size: 662 369 295 BtgI C'CryG_G Cuts at: 0 111 134 202 211 393 464 476 1326 Size: 111 23 68 9 182 71 12 850 Fragments arranged by size: 850 182 111 71 68 23 12 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 114 1326 Size: 114 1212 BtsI GCAGTG_nn' Cuts at: 0 1208 1326 Size: 1208 118 EaeI y'GGCC_r Cuts at: 0 108 1326 Size: 108 1218 EagI C'GGCC_G Cuts at: 0 108 1326 Size: 108 1218 EarI CTCTTCn'nnn_ Cuts at: 0 988 1108 1326 Size: 988 120 218 Fragments arranged by size: 988 218 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 298 484 1326 Size: 298 186 842 Fragments arranged by size: 842 298 186 EcoICRI GAG'CTC Cuts at: 0 998 1326 Size: 998 328 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 562 577 836 980 1326 Size: 331 231 15 259 144 346 Fragments arranged by size: 346 331 259 231 144 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 442 1326 Size: 442 884 EcoO109I rG'GnC_Cy Cuts at: 0 35 36 373 447 533 675 1326 Size: 35 1 337 74 86 142 651 Fragments arranged by size: 651 337 142 86 74 35 1 HaeII r_GCGC'y Cuts at: 0 7 498 1326 Size: 7 491 828 Fragments arranged by size: 828 491 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 319 351 374 406 418 439 450 471 Size: 319 32 23 32 12 21 11 21 Cuts at: 471 938 953 970 985 1326 Size: 467 15 17 15 341 Fragments arranged by size: 467 341 319 32 32 23 21 21 17 15 15 12 11 HindIII A'AGCT_T Cuts at: 0 1080 1326 Size: 1080 246 KasI G'GCGC_C Cuts at: 0 3 1326 Size: 3 1323 MluI A'CGCG_T Cuts at: 0 410 1326 Size: 410 916 MslI CAynn'nnrTG Cuts at: 0 94 133 1326 Size: 94 39 1193 Fragments arranged by size: 1193 94 39 MspA1I CmG'CkG Cuts at: 0 94 113 204 395 466 717 799 1052 Size: 94 19 91 191 71 251 82 253 Cuts at: 1052 1326 Size: 274 Fragments arranged by size: 274 253 251 191 94 91 82 71 19 NarI GG'CG_CC Cuts at: 0 4 1326 Size: 4 1322 NspI r_CATG'y Cuts at: 0 839 1170 1326 Size: 839 331 156 Fragments arranged by size: 839 331 156 PasI CC'CwG_GG Cuts at: 0 678 1326 Size: 678 648 PciI A'CATG_T Cuts at: 0 1166 1326 Size: 1166 160 PfoI T'CCnGG_A Cuts at: 0 457 1326 Size: 457 869 PpuMI rG'GwC_Cy Cuts at: 0 447 533 1326 Size: 447 86 793 Fragments arranged by size: 793 447 86 PspOMI G'GGCC_C Cuts at: 0 35 373 674 1326 Size: 35 338 301 652 Fragments arranged by size: 652 338 301 35 PstI C_TGCA'G Cuts at: 0 771 1035 1326 Size: 771 264 291 Fragments arranged by size: 771 291 264 SacI G_AGCT'C Cuts at: 0 1000 1326 Size: 1000 326 SacII CC_GC'GG Cuts at: 0 114 205 396 467 1326 Size: 114 91 191 71 859 Fragments arranged by size: 859 191 114 91 71 SapI GCTCTTCn'nnn_ Cuts at: 0 988 1108 1326 Size: 988 120 218 Fragments arranged by size: 988 218 120 SbfI CC_TGCA'GG Cuts at: 0 771 1326 Size: 771 555 SfcI C'TryA_G Cuts at: 0 598 767 1031 1326 Size: 598 169 264 295 Fragments arranged by size: 598 295 264 169 SfoI GGC'GCC Cuts at: 0 5 1326 Size: 5 1321 SmlI C'TyrA_G Cuts at: 0 75 1326 Size: 75 1251 SnaBI TAC'GTA Cuts at: 0 144 1326 Size: 144 1182 StuI AGG'CCT Cuts at: 0 765 1326 Size: 765 561 StyI C'CwwG_G Cuts at: 0 263 942 1107 1326 Size: 263 679 165 219 Fragments arranged by size: 679 263 219 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 64 204 299 505 1150 1326 Size: 64 140 95 206 645 176 Fragments arranged by size: 645 206 176 140 95 64 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 535 567 1122 1146 1154 1178 1326 Size: 535 32 555 24 8 24 148 Fragments arranged by size: 555 535 148 32 24 24 8 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 328 355 912 1326 Size: 328 27 557 414 Fragments arranged by size: 557 414 328 27 Enzymes that do cut: AccI AfeI AflIII AleI ApaI BanI BanII BbeI BcgI BglI Bme1580I BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BssHII BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI MluI MslI MspA1I NarI NspI PasI PciI PfoI PpuMI PspOMI PstI SacI SacII SapI SbfI SfcI SfoI SmlI SnaBI StuI StyI TaqII TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BsaBI BsmI BspHI BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI