(Circular) (Six-base) MAPSORT of: pFN21AE4189.seq Check: 8303 from: 1 to: 6730 pFN21AE4189 6730 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4540 278 Size: 53 83 186 936 3004 2468 Fragments arranged by size: 3004 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2364 4073 1937 Size: 427 1709 4594 Fragments arranged by size: 4594 1709 427 Acc65I G'GTAC_C Cuts at: 2526 4051 2526 Size: 1525 5205 AclI AA'CG_TT Cuts at: 1563 4857 5230 1563 Size: 3294 373 3063 Fragments arranged by size: 3294 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2463 2576 2786 2882 2896 3355 4793 6177 Size: 831 113 210 96 14 459 1438 1384 Cuts at: 6177 6445 1632 Size: 268 1917 Fragments arranged by size: 1917 1438 1384 831 459 268 210 113 96 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6533 AflIII A'CryG_T Cuts at: 1203 2403 5629 6606 1203 Size: 1200 3226 977 1327 Fragments arranged by size: 3226 1327 1200 977 AgeI A'CCGG_T Cuts at: 5561 5561 Size: 6730 AhdI GACnn_n'nnGTC Cuts at: 5459 5459 Size: 6730 AleI CACnn'nnGTG Cuts at: 1166 3275 1166 Size: 2109 4621 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6730 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2201 2233 2201 Size: 32 6698 AlwNI CAG_nnn'CTG Cuts at: 2101 2567 2764 3043 3082 3253 3365 3485 4473 Size: 466 197 279 39 171 112 120 988 Cuts at: 4473 6045 2101 Size: 1572 2786 Fragments arranged by size: 2786 1572 988 466 279 197 171 120 112 39 ApaLI G'TGCA_C Cuts at: 3268 4787 5943 3268 Size: 1519 1156 4055 Fragments arranged by size: 4055 1519 1156 ApoI r'AATT_y Cuts at: 1493 2330 2807 3005 4039 4265 4417 6526 1493 Size: 837 477 198 1034 226 152 2109 1697 Fragments arranged by size: 2109 1697 1034 837 477 226 198 152 AseI AT'TA_AT Cuts at: 160 1032 5284 160 Size: 872 4252 1606 Fragments arranged by size: 4252 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 AvaI C'yCGr_G Cuts at: 1870 1943 2942 3588 4055 1870 Size: 73 999 646 467 4545 Fragments arranged by size: 4545 999 646 467 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3686 3719 3686 Size: 33 6697 BamHI G'GATC_C Cuts at: 4060 4427 4060 Size: 367 6363 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2461 2526 4051 5506 618 Size: 325 217 627 674 65 1525 1455 1842 Fragments arranged by size: 1842 1525 1455 674 627 325 217 65 BanII G_rGCy'C Cuts at: 1391 2118 3222 3732 3783 3994 4001 4049 1391 Size: 727 1104 510 51 211 7 48 4072 Fragments arranged by size: 4072 1104 727 510 211 51 48 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2747 2805 928 Size: 971 848 58 4853 Fragments arranged by size: 4853 971 848 58 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2933 2967 3183 3217 4921 4955 1903 Size: 34 996 34 216 34 1704 34 3678 Fragments arranged by size: 3678 1704 996 216 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4622 5832 4622 Size: 1210 5520 BclI T'GATC_A Cuts at: 1548 3084 1548 Size: 1536 5194 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5341 136 Size: 107 122 71 631 605 3669 1525 Fragments arranged by size: 3669 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2203 6725 2203 Size: 4522 2208 BlpI GC'TnA_GC Cuts at: 2753 3216 4147 2753 Size: 463 931 5336 Fragments arranged by size: 5336 931 463 Bme1580I G_kGCm'C Cuts at: 1790 3272 3801 4791 5947 1790 Size: 1482 529 990 1156 2573 Fragments arranged by size: 2573 1482 1156 990 529 BmgBI CAC'GTC Cuts at: 1318 2620 3355 1318 Size: 1302 735 4693 Fragments arranged by size: 4693 1302 735 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3404 3573 3711 Size: 416 53 83 399 24 1987 169 138 Cuts at: 3711 3995 4461 5419 442 Size: 284 466 958 1753 Fragments arranged by size: 1987 1753 958 466 416 399 284 169 138 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6730 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2050 2299 2050 Size: 249 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2135 2168 2573 2597 3161 3269 3920 Size: 781 115 33 405 24 564 108 651 Cuts at: 3920 4036 5390 1239 Size: 116 1354 2579 Fragments arranged by size: 2579 1354 781 651 564 405 116 115 108 33 24 Bpu10I CC'TnA_GC Cuts at: 3509 3509 Size: 6730 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3011 4855 5720 6018 3011 Size: 1844 865 298 3723 Fragments arranged by size: 3723 1844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2137 2973 3425 5393 882 Size: 617 638 836 452 1968 2219 Fragments arranged by size: 2219 1968 836 638 617 452 BsaAI yAC'GTr Cuts at: 493 1204 2406 4521 493 Size: 711 1202 2115 2702 Fragments arranged by size: 2702 2115 1202 711 BsaBI GATnn'nnATC Cuts at: 6724 6724 Size: 6730 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4537 4919 5554 275 Size: 53 83 186 936 3004 382 635 1451 Fragments arranged by size: 3004 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2050 2409 3775 5163 5561 5835 5982 6393 2050 Size: 359 1366 1388 398 274 147 411 2387 Fragments arranged by size: 2387 1388 1366 411 398 359 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2717 2747 3456 3486 1178 Size: 30 165 30 1314 30 709 30 4422 Fragments arranged by size: 4422 1314 709 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 3182 3740 1186 Size: 1168 828 558 4176 Fragments arranged by size: 4176 1168 828 558 BseYI C'CCAG_C Cuts at: 3253 3336 3686 3782 3787 5933 6429 3253 Size: 83 350 96 5 2146 496 3554 Fragments arranged by size: 3554 2146 496 350 96 83 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2324 2978 3067 3467 3518 3741 1163 Size: 573 588 654 89 400 51 223 4152 Fragments arranged by size: 4152 654 588 573 400 223 89 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3643 4180 4941 5969 1540 Size: 275 186 1642 537 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 537 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 3222 3272 3732 4791 4876 5947 2118 Size: 1104 50 460 1059 85 1071 2901 Fragments arranged by size: 2901 1104 1071 1059 460 85 50 BsmI GAATG_Cn' Cuts at: 4241 4334 4241 Size: 93 6637 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 3222 3272 3732 3783 3801 3994 Size: 399 328 1104 50 460 51 18 193 Cuts at: 3994 4001 4049 4791 4876 5947 1391 Size: 7 48 742 85 1071 2174 Fragments arranged by size: 2174 1104 1071 742 460 399 328 193 85 51 50 48 18 7 BspEI T'CCGG_A Cuts at: 2050 6393 2050 Size: 4343 2387 BspHI T'CATG_A Cuts at: 4619 6349 4619 Size: 1730 5000 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3504 4085 844 Size: 2660 581 3489 Fragments arranged by size: 3489 2660 581 BsrBI CCG'CTC Cuts at: 1394 1823 2704 3121 4617 1394 Size: 429 881 417 1496 3507 Fragments arranged by size: 3507 1496 881 429 417 BsrDI GCAATG_nn' Cuts at: 65 1248 5225 5399 65 Size: 1183 3977 174 1396 Fragments arranged by size: 3977 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3682 5374 5561 1700 Size: 110 1872 1692 187 2869 Fragments arranged by size: 2869 1872 1692 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6730 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6730 BssSI C'ACGA_G Cuts at: 4790 5802 4790 Size: 1012 5718 BstAPI GCAn_nnn'nTGC Cuts at: 794 3310 794 Size: 2516 4214 BstBI TT'CG_AA Cuts at: 5534 5534 Size: 6730 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6730 BstXI CCAn_nnnn'nTGG Cuts at: 4008 4008 Size: 6730 BstYI r'GATC_y Cuts at: 1972 2203 2474 4060 4427 4812 4829 6281 6367 Size: 231 271 1586 367 385 17 1452 86 Cuts at: 6367 6379 6725 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1586 1452 385 367 346 271 231 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5239 Fragments arranged by size: 5239 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5991 Fragments arranged by size: 5991 683 56 BtsI GCAGTG_nn' Cuts at: 2531 2961 3157 3254 4246 5040 5060 2531 Size: 430 196 97 992 794 20 4201 Fragments arranged by size: 4201 992 794 430 196 97 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6730 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3538 3573 529 Size: 35 2974 35 3686 Fragments arranged by size: 3686 2974 35 35 DraI TTT'AAA Cuts at: 4034 4034 Size: 6730 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2129 5737 809 Size: 507 813 3608 1802 Fragments arranged by size: 3608 1802 813 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3054 3603 Size: 54 1425 50 275 18 177 1047 549 Cuts at: 3603 3640 3846 3984 4177 5066 6695 8 Size: 37 206 138 193 889 1629 43 Fragments arranged by size: 1629 1425 1047 889 549 275 206 193 177 138 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 4177 1537 Size: 275 1828 537 4090 Fragments arranged by size: 4090 1828 537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 2817 3123 4660 1353 Size: 933 531 306 1537 3423 Fragments arranged by size: 3423 1537 933 531 306 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5317 5691 5837 387 Size: 1064 138 3728 374 146 1280 Fragments arranged by size: 3728 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2116 3220 3730 2116 Size: 1104 510 5116 Fragments arranged by size: 5116 1104 510 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2135 2168 2463 2573 2576 2597 Size: 393 388 115 33 295 110 3 21 Cuts at: 2597 2786 2882 2896 3161 3269 3355 3920 4036 Size: 189 96 14 265 108 86 565 116 Cuts at: 4036 4793 5390 6177 6445 1239 Size: 757 597 787 268 1524 Fragments arranged by size: 1524 787 757 597 565 393 388 295 268 265 189 116 115 110 108 96 86 33 21 14 3 EcoO109I rG'GnC_Cy Cuts at: 2067 2948 3292 2067 Size: 881 344 5505 Fragments arranged by size: 5505 881 344 EcoRI G'AATT_C Cuts at: 2330 4039 4417 2330 Size: 1709 378 4643 Fragments arranged by size: 4643 1709 378 EcoRV GAT'ATC Cuts at: 2236 2236 Size: 6730 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3083 3083 Size: 6730 FspI TGC'GCA Cuts at: 2651 5236 2651 Size: 2585 4145 HaeII r_GCGC'y Cuts at: 1678 5877 1678 Size: 4199 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2066 2098 3553 Size: 32 135 32 443 32 19 32 1455 Cuts at: 3553 3571 3585 3603 5371 5403 5445 5477 1373 Size: 18 14 18 1768 32 42 32 2626 Fragments arranged by size: 2626 1768 1455 443 135 42 32 32 32 32 32 32 19 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 4074 4320 669 Size: 988 281 2136 246 3079 Fragments arranged by size: 3079 2136 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6730 HpaI GTT'AAC Cuts at: 4320 4320 Size: 6730 KpnI G_GTAC'C Cuts at: 2530 4055 2530 Size: 1525 5205 MfeI C'AATT_G Cuts at: 4329 4329 Size: 6730 MluI A'CGCG_T Cuts at: 6606 6606 Size: 6730 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2325 5844 6028 1305 Size: 1020 3519 184 2007 Fragments arranged by size: 3519 2007 1020 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 3056 3605 3848 3986 Size: 54 1425 343 177 1047 549 243 138 Cuts at: 3986 10 Size: 2754 Fragments arranged by size: 2754 1425 1047 549 343 243 177 138 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 3275 4688 5047 5206 518 Size: 648 1077 1032 1413 359 159 2042 Fragments arranged by size: 2042 1413 1077 1032 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2431 2689 2761 3238 3340 3639 3645 3700 Size: 825 258 72 477 102 299 6 55 Cuts at: 3700 4146 4823 5971 6216 1606 Size: 446 677 1148 245 2120 Fragments arranged by size: 2120 1148 825 677 477 446 299 258 245 102 72 55 6 NaeI GCC'GGC Cuts at: 3684 3684 Size: 6730 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5239 Fragments arranged by size: 5239 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6730 NgoMIV G'CCGG_C Cuts at: 3682 3682 Size: 6730 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6730 NotI GC'GGCC_GC Cuts at: 3640 4177 3640 Size: 537 6193 NspI r_CATG'y Cuts at: 1132 1229 2149 4088 5633 1132 Size: 97 920 1939 1545 2229 Fragments arranged by size: 2229 1939 1545 920 97 PasI CC'CwG_GG Cuts at: 1348 3524 1348 Size: 2176 4554 PciI A'CATG_T Cuts at: 5629 5629 Size: 6730 PflMI CCAn_nnn'nTGG Cuts at: 1108 3601 1108 Size: 2493 4237 PfoI T'CCnGG_A Cuts at: 2109 2550 2613 2697 3897 2109 Size: 441 63 84 1200 4942 Fragments arranged by size: 4942 1200 441 84 63 PmeI GTTT'AAAC Cuts at: 4034 4034 Size: 6730 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4799 4831 6312 6344 4799 Size: 32 1481 32 5185 Fragments arranged by size: 5185 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3292 3292 Size: 6730 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6730 PsiI TTA'TAA Cuts at: 4300 4300 Size: 6730 PstI C_TGCA'G Cuts at: 830 2274 2430 2514 2544 2898 2956 3315 3876 Size: 1444 156 84 30 354 58 359 561 Cuts at: 3876 4082 830 Size: 206 3478 Fragments arranged by size: 3478 1444 561 359 354 206 156 84 58 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6730 PvuII CAG'CTG Cuts at: 2689 2761 3238 3340 3700 2689 Size: 72 477 102 360 5719 Fragments arranged by size: 5719 477 360 102 72 SacI G_AGCT'C Cuts at: 2118 3222 3732 2118 Size: 1104 510 5116 Fragments arranged by size: 5116 1104 510 SalI G'TCGA_C Cuts at: 1936 4072 1936 Size: 2136 4594 SbfI CC_TGCA'GG Cuts at: 3876 4082 3876 Size: 206 6524 ScaI AGT'ACT Cuts at: 4978 6690 4978 Size: 1712 5018 SexAI A'CCwGG_T Cuts at: 2522 3537 3819 2522 Size: 1015 282 5433 Fragments arranged by size: 5433 1015 282 SfcI C'TryA_G Cuts at: 826 1045 2270 2426 2510 2540 2894 2952 3311 Size: 219 1225 156 84 30 354 58 359 Cuts at: 3311 3695 3872 4078 5213 5894 6085 826 Size: 384 177 206 1135 681 191 1471 Fragments arranged by size: 1471 1225 1135 681 384 359 354 219 206 191 177 156 84 58 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6730 SmaI CCC'GGG Cuts at: 1872 4057 1872 Size: 2185 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2990 4834 5735 5997 820 Size: 197 926 1047 1844 901 262 1553 Fragments arranged by size: 1844 1553 1047 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4521 493 Size: 4028 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6730 SphI G_CATG'C Cuts at: 1132 4088 1132 Size: 2956 3774 SspI AAT'ATT Cuts at: 5 52 4654 5 Size: 47 4602 2081 Fragments arranged by size: 4602 2081 47 StuI AGG'CCT Cuts at: 1517 2387 2484 2996 3027 1517 Size: 870 97 512 31 5220 Fragments arranged by size: 5220 870 512 97 31 StyI C'CwwG_G Cuts at: 513 1065 2004 2286 2676 4169 513 Size: 552 939 282 390 1493 3074 Fragments arranged by size: 3074 1493 939 552 390 282 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2827 4757 4774 4927 5112 5557 325 Size: 1255 1247 1930 17 153 185 445 1498 Fragments arranged by size: 1930 1498 1255 1247 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2640 2670 4976 Size: 274 80 33 51 1443 663 30 2306 Cuts at: 4976 6688 96 Size: 1712 138 Fragments arranged by size: 2306 1712 1443 663 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2717 2749 3456 3488 6690 6722 2717 Size: 32 707 32 3202 32 2725 Fragments arranged by size: 3202 2725 707 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6730 XbaI T'CTAG_A Cuts at: 4066 4066 Size: 6730 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6730 XmaI C'CCGG_G Cuts at: 1870 4055 1870 Size: 2185 4545 XmnI GAAnn'nnTTC Cuts at: 4859 4859 Size: 6730 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4538 276 Size: 53 83 186 936 3004 2468 Fragments arranged by size: 3004 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI