################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Aor51HI AscI Asp718I AspEI AvrII BfrI BlnI BsaI BsaWI BseAI BsiWI Bsp1407I BspEI BspLU11I BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraIII Eam1105I Eco47III EcoO65I EcoRI EcoT22I FseI KpnI MluI MroI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflIII 3937 ACGTGT A^C[GA][CT]GT AlwNI 3090 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1778 GGGCCC GGGCC^C AseI 2931 ATTAAT AT^TAAT AsnI 2931 ATTAAT AT^TAAT Asp700I 3038 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 185 TGCGCA TGC^GCA BalI 2792 TGGCCA TGG^CCA BamHI 2179 GGATCC G^GATCC BbvCI 3616 CCTCAGC CCTCAGC(-5/-2) BcgI 3979 GCAGGGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3663 GGATAC GTATCC(6/5) BclI 551 TGATCA T^GATCA BsaBI 3508 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 4347 GAATGC GAATGC(1/-1) BspDI 4413 ATCGAT AT^CGAT BspHI 1491 TCATGA T^CATGA BspMI 3624 GCAGGT ACCTGC(4/8) BsrDI 3580 GCAATG GCAATG(2/0) BssHII 104 GCGCGC G^CGCGC BssSI 1507 CACGAG CACGAG(-5/-1) BstAPI 4399 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 4413 ATCGAT AT^CGAT EagI 419 CGGCCG C^GGCCG EclXI 419 CGGCCG C^GGCCG Eco52I 419 CGGCCG C^GGCCG EcoRV 3643 GATATC GAT^ATC FbaI 551 TGATCA T^GATCA FspI 185 TGCGCA TGC^GCA HindIII 3560 AAGCTT A^AGCTT HpaI 922 GTTAAC GTT^AAC MamI 3508 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2792 TGGCCA TGG^CCA MscI 2792 TGGCCA TGG^CCA NaeI 252 GCCGGC GCC^GGC NgoMIV 252 GCCGGC G^CCGGC PaeR7I 405 CTCGAG C^TCGAG PshAI 3822 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2931 ATTAAT AT^TAAT RcaI 1491 TCATGA T^CATGA SacI 3379 GAGCTC GAGCT^C StuI 3747 AGGCCT AGG^CCT XbaI 1720 TCTAGA T^CTAGA XhoI 405 CTCGAG C^TCGAG XmnI 3038 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1939 GTCTAC GT^[AC][GT]AC 2050 GTAGAC Alw44I 1046 GTGCAC G^TGCAC 2709 GTGCAC ApaLI 1046 GTGCAC G^TGCAC 2709 GTGCAC AspI 743 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1933 GACCCTGTC BbrPI 1049 CACGTG CAC^GTG 3936 CACGTG BglII 1474 AGATCT A^GATCT 1486 AGATCT BlpI 1669 GCTGAGC GC^T[ACGT]AGC 2350 GCTGAGC Bpu1102I 1669 GCTGAGC GC^T[ACGT]AGC 2350 GCTGAGC BsaAI 1049 CACGTG [CT]AC^GT[GA] 3936 CACGTG BsiEI 419 CGGCCG CG[GA][CT]^CG 480 CGGTCG BsmBI 458 CGTCTC CGTCTC(1/5) 3590 CGTCTC BsrBI 229 CCGCTC CCGCTC(-3/-3) 157 GAGCGG BstXI 1117 CCAATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2603 CCATCCAGTTGG CelII 1669 GCTGAGC GC^T[ACGT]AGC 2350 GCTGAGC DraI 1137 TTTAAA TTT^AAA 1962 TTTAAA DrdI 264 GACTTCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2882 GACCCCACAGTC EcoNI 64 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3193 CCTACCCCAGG PflFI 743 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1933 GACCCTGTC PflMI 315 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2424 CCACTCAATGG PmaCI 1049 CACGTG CAC^GTG 3936 CACGTG PmlI 1049 CACGTG CAC^GTG 3936 CACGTG PpuMI 2808 AGGACCT [GA]G^G[AT]CC[CT] 3398 AGGACCT ScaI 1273 AGTACT AGT^ACT 2332 AGTACT SmaI 42 CCCGGG CCC^GGG 427 CCCGGG SphI 1346 GCATGC GCATG^C 1900 GCATGC Tsp45I 3323 GTGAC ^GT[GC]AC 3820 GTGAC Tth111I 743 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1933 GACCCTGTC Van91I 315 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2424 CCACTCAATGG XmaCI 42 CCCGGG C^CCGGG 427 CCCGGG XmaI 42 CCCGGG C^CCGGG 427 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AocI ApoI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI XhoII