(Circular) (Six-base) MAPSORT of: pFN21AE3472.seq Check: 3466 from: 1 to: 9241 pFN21AE3472 9241 bp With 182 enzymes: SgfI * July 13, 2017 17:10 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7051 278 Size: 53 83 186 936 5515 2468 Fragments arranged by size: 5515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3945 4056 6584 1937 Size: 2008 111 2528 4594 Fragments arranged by size: 4594 2528 2008 111 Acc65I G'GTAC_C Cuts at: 6562 6562 Size: 9241 AclI AA'CG_TT Cuts at: 1563 7368 7741 1563 Size: 5805 373 3063 Fragments arranged by size: 5805 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2591 3099 4016 5006 5574 6533 7304 8688 Size: 959 508 917 990 568 959 771 1384 Cuts at: 8688 8956 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 990 959 959 917 771 568 508 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9044 AflIII A'CryG_T Cuts at: 1203 5942 8140 9117 1203 Size: 4739 2198 977 1327 Fragments arranged by size: 4739 2198 1327 977 AgeI A'CCGG_T Cuts at: 8072 8072 Size: 9241 AhdI GACnn_n'nnGTC Cuts at: 7970 7970 Size: 9241 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9241 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5252 5284 6048 6080 5252 Size: 32 764 32 8413 Fragments arranged by size: 8413 764 32 32 AlwNI CAG_nnn'CTG Cuts at: 5100 6984 8556 5100 Size: 1884 1572 5785 Fragments arranged by size: 5785 1884 1572 ApaI G_GGCC'C Cuts at: 3787 3787 Size: 9241 ApaLI G'TGCA_C Cuts at: 3051 4714 7298 8454 3051 Size: 1663 2584 1156 3838 Fragments arranged by size: 3838 2584 1663 1156 ApoI r'AATT_y Cuts at: 1493 3148 3253 3817 4304 4316 6550 6776 6928 Size: 1655 105 564 487 12 2234 226 152 Cuts at: 6928 9037 1493 Size: 2109 1697 Fragments arranged by size: 2234 2109 1697 1655 564 487 226 152 105 12 AseI AT'TA_AT Cuts at: 160 1032 4937 7795 160 Size: 872 3905 2858 1606 Fragments arranged by size: 3905 2858 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9241 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2410 2432 3844 6566 1870 Size: 73 104 363 22 1412 2722 4545 Fragments arranged by size: 4545 2722 1412 363 104 73 22 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4476 4509 5427 5460 4476 Size: 33 918 33 8257 Fragments arranged by size: 8257 918 33 33 BamHI G'GATC_C Cuts at: 4184 6571 6938 4184 Size: 2387 367 6487 Fragments arranged by size: 6487 2387 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2332 2366 2392 2405 Size: 325 217 627 264 281 34 26 13 Cuts at: 2405 2446 6119 6253 6562 8017 618 Size: 41 3673 134 309 1455 1842 Fragments arranged by size: 3673 1842 1455 627 325 309 281 264 217 134 41 34 26 13 BanII G_rGCy'C Cuts at: 1391 2069 2095 2289 2433 2875 3787 5388 6560 Size: 678 26 194 144 442 912 1601 1172 Cuts at: 6560 1391 Size: 4072 Fragments arranged by size: 4072 1601 1172 912 678 442 194 144 26 BbeI G_GCGC'C Cuts at: 2055 2370 2396 2409 2450 2055 Size: 315 26 13 41 8846 Fragments arranged by size: 8846 315 41 26 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3390 3861 4632 5727 928 Size: 971 1491 471 771 1095 4442 Fragments arranged by size: 4442 1491 1095 971 771 471 BbvCI CC'TCA_GC Cuts at: 5622 5622 Size: 9241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5973 6007 7432 7466 1903 Size: 34 4036 34 1425 34 3678 Fragments arranged by size: 4036 3678 1425 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5662 7133 8343 5662 Size: 1471 1210 6560 Fragments arranged by size: 6560 1471 1210 BclI T'GATC_A Cuts at: 1548 2556 1548 Size: 1008 8233 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2099 2364 Size: 107 122 71 631 605 337 90 265 Cuts at: 2364 6188 7852 136 Size: 3824 1664 1525 Fragments arranged by size: 3824 1664 1525 631 605 337 265 122 107 90 71 BglII A'GATC_T Cuts at: 3479 3491 9236 3479 Size: 12 5745 3484 Fragments arranged by size: 5745 3484 12 BlpI GC'TnA_GC Cuts at: 3675 4356 6658 3675 Size: 681 2302 6258 Fragments arranged by size: 6258 2302 681 Bme1580I G_kGCm'C Cuts at: 1790 3055 3787 4718 7302 8458 1790 Size: 1265 732 931 2584 1156 2573 Fragments arranged by size: 2584 2573 1265 1156 931 732 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2648 2825 5129 Size: 416 53 83 399 24 1231 177 2304 Cuts at: 5129 5319 5342 6242 6972 7930 442 Size: 190 23 900 730 958 1753 Fragments arranged by size: 2304 1753 1231 958 900 730 416 399 190 177 83 53 24 23 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9241 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2111 5407 2111 Size: 3296 5945 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2685 2787 4028 5149 5377 7901 1239 Size: 1446 102 1241 1121 228 2524 2579 Fragments arranged by size: 2579 2524 1446 1241 1121 228 102 Bpu10I CC'TnA_GC Cuts at: 2226 5622 2226 Size: 3396 5845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2081 3181 4486 5769 7366 8231 8529 2081 Size: 1100 1305 1283 1597 865 298 2793 Fragments arranged by size: 2793 1597 1305 1283 1100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7904 882 Size: 617 6405 2219 Fragments arranged by size: 6405 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3056 5943 7032 493 Size: 711 1852 2887 1089 2702 Fragments arranged by size: 2887 2702 1852 1089 711 BsaBI GATnn'nnATC Cuts at: 5517 9235 5517 Size: 3718 5523 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 2367 2393 2406 Size: 53 83 186 936 519 315 26 13 Cuts at: 2406 2447 3760 7048 7430 8065 275 Size: 41 1313 3288 382 635 1451 Fragments arranged by size: 3288 1451 1313 936 635 519 382 315 186 83 53 41 26 13 BsaWI w'CCGG_w Cuts at: 7674 8072 8346 8493 8904 7674 Size: 398 274 147 411 8011 Fragments arranged by size: 8011 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3882 3912 6048 6078 6397 Size: 30 165 30 2479 30 2136 30 319 Cuts at: 6397 6427 1178 Size: 30 3992 Fragments arranged by size: 3992 2479 2136 319 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2103 2254 3009 4900 5049 5369 1186 Size: 917 151 755 1891 149 320 5058 Fragments arranged by size: 5058 1891 917 755 320 151 149 BseYI C'CCAG_C Cuts at: 8444 8940 8444 Size: 496 8745 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3389 3540 4098 6228 1163 Size: 573 1653 151 558 2130 4176 Fragments arranged by size: 4176 2130 1653 573 558 151 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2427 2488 6691 7452 8480 1540 Size: 275 186 426 61 4203 761 1028 2301 Fragments arranged by size: 4203 2301 1028 761 426 275 186 61 BsiHKAI G_wGCw'C Cuts at: 3055 3812 4718 5388 7302 7387 8458 3055 Size: 757 906 670 1914 85 1071 3838 Fragments arranged by size: 3838 1914 1071 906 757 670 85 BsmI GAATG_Cn' Cuts at: 6358 6752 6845 6358 Size: 394 93 8754 Fragments arranged by size: 8754 394 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2469 5601 2469 Size: 3132 6109 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2095 2289 2433 2875 3055 3787 Size: 399 279 26 194 144 442 180 732 Cuts at: 3787 3812 4718 5388 6560 7302 7387 8458 1391 Size: 25 906 670 1172 742 85 1071 2174 Fragments arranged by size: 2174 1172 1071 906 742 732 670 442 399 279 194 180 144 85 26 25 BspEI T'CCGG_A Cuts at: 8904 8904 Size: 9241 BspHI T'CATG_A Cuts at: 3496 7130 8860 3496 Size: 3634 1730 3877 Fragments arranged by size: 3877 3634 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5620 6596 844 Size: 4776 976 3489 Fragments arranged by size: 4776 3489 976 BsrBI CCG'CTC Cuts at: 1394 1823 2164 2236 7128 1394 Size: 429 341 72 4892 3507 Fragments arranged by size: 4892 3507 429 341 72 BsrDI GCAATG_nn' Cuts at: 65 1248 5592 7736 7910 65 Size: 1183 4344 2144 174 1396 Fragments arranged by size: 4344 2144 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2257 2312 2483 7885 8072 1700 Size: 110 447 55 171 5402 187 2869 Fragments arranged by size: 5402 2869 447 187 171 110 55 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9241 BssHII G'CGCG_C Cuts at: 1925 2109 1925 Size: 184 9057 BssSI C'ACGA_G Cuts at: 3512 7301 8313 3512 Size: 3789 1012 4440 Fragments arranged by size: 4440 3789 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6410 794 Size: 5616 3625 BstBI TT'CG_AA Cuts at: 8045 8045 Size: 9241 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9241 BstXI CCAn_nnnn'nTGG Cuts at: 3129 4615 3129 Size: 1486 7755 BstYI r'GATC_y Cuts at: 1972 2669 3479 3491 4184 4458 5227 6571 6938 Size: 697 810 12 693 274 769 1344 367 Cuts at: 6938 7323 7340 8792 8878 8890 9236 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1344 810 769 697 693 385 367 346 274 86 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2070 2907 3755 6258 6483 2070 Size: 837 848 2503 225 4828 Fragments arranged by size: 4828 2503 848 837 225 BtgI C'CryG_G Cuts at: 513 1065 2004 2115 2124 2289 2344 2359 3680 Size: 552 939 111 9 165 55 15 1321 Cuts at: 3680 5580 6067 6347 513 Size: 1900 487 280 3407 Fragments arranged by size: 3407 1900 1321 939 552 487 280 165 111 55 15 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8502 Fragments arranged by size: 8502 683 56 BtsI GCAGTG_nn' Cuts at: 3412 3838 4992 6757 7551 7571 3412 Size: 426 1154 1765 794 20 5082 Fragments arranged by size: 5082 1765 1154 794 426 20 ClaI AT'CG_AT Cuts at: 1554 6419 1554 Size: 4865 4376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3510 3545 529 Size: 35 2946 35 6225 Fragments arranged by size: 6225 2946 35 35 DraI TTT'AAA Cuts at: 3144 3969 6545 3144 Size: 825 2576 5840 Fragments arranged by size: 5840 2576 825 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2275 4893 8248 809 Size: 507 959 2618 3355 1802 Fragments arranged by size: 3355 2618 1802 959 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2424 4270 4797 Size: 54 1425 50 275 18 594 1846 527 Cuts at: 4797 6688 7577 9206 8 Size: 1891 889 1629 43 Fragments arranged by size: 1891 1846 1629 1425 889 594 527 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2424 6688 1537 Size: 275 612 4264 4090 Fragments arranged by size: 4264 4090 612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2243 2863 3312 3429 5877 5945 5972 6465 Size: 890 620 449 117 2448 68 27 493 Cuts at: 6465 7171 1353 Size: 706 3423 Fragments arranged by size: 3423 2448 890 706 620 493 449 117 68 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2100 2183 7828 8202 8348 387 Size: 1064 138 511 83 5645 374 146 1280 Fragments arranged by size: 5645 1280 1064 511 374 146 138 83 EcoICRI GAG'CTC Cuts at: 5386 5386 Size: 9241 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2591 2685 2787 3099 4016 4028 5006 Size: 393 959 94 102 312 917 12 978 Cuts at: 5006 5149 5377 5574 6533 7304 7901 8688 8956 Size: 143 228 197 959 771 597 787 268 Cuts at: 8956 1239 Size: 1524 Fragments arranged by size: 1524 978 959 959 917 787 771 597 393 312 268 228 197 143 102 94 12 EcoNI CCTnn'n_nnAGG Cuts at: 2073 5202 2073 Size: 3129 6112 EcoO109I rG'GnC_Cy Cuts at: 2630 2972 3783 3784 4814 5404 2630 Size: 342 811 1 1030 590 6467 Fragments arranged by size: 6467 1030 811 590 342 1 EcoRI G'AATT_C Cuts at: 6550 6928 6550 Size: 378 8863 EcoRV GAT'ATC Cuts at: 5650 5650 Size: 9241 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2742 2892 3386 4283 2742 Size: 150 494 897 7700 Fragments arranged by size: 7700 897 494 150 FspI TGC'GCA Cuts at: 2192 7747 2192 Size: 5555 3686 HaeII r_GCGC'y Cuts at: 1678 2055 2370 2396 2409 2450 8388 1678 Size: 377 315 26 13 41 5938 2531 Fragments arranged by size: 5938 2531 377 315 41 26 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2521 2553 2876 2908 3193 Size: 32 135 32 949 32 323 32 285 Cuts at: 3193 3225 5239 5271 5358 5390 5634 5666 5887 Size: 32 2014 32 87 32 244 32 221 Cuts at: 5887 5919 7882 7914 7956 7988 1373 Size: 32 1963 32 42 32 2626 Fragments arranged by size: 2626 2014 1963 949 323 285 244 221 135 87 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2929 4643 4956 5744 6275 6585 Size: 988 281 991 1714 313 788 531 310 Cuts at: 6585 6831 669 Size: 246 3079 Fragments arranged by size: 3079 1714 991 988 788 531 313 310 281 246 HindIII A'AGCT_T Cuts at: 748 5565 748 Size: 4817 4424 HpaI GTT'AAC Cuts at: 2929 6831 2929 Size: 3902 5339 KasI G'GCGC_C Cuts at: 2051 2366 2392 2405 2446 2051 Size: 315 26 13 41 8846 Fragments arranged by size: 8846 315 41 26 13 KpnI G_GTAC'C Cuts at: 6566 6566 Size: 9241 MfeI C'AATT_G Cuts at: 4252 5574 6240 6840 4252 Size: 1322 666 600 6653 Fragments arranged by size: 6653 1322 666 600 MluI A'CGCG_T Cuts at: 9117 9117 Size: 9241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2589 4104 4281 8355 8539 1305 Size: 1284 1515 177 4074 184 2007 Fragments arranged by size: 4074 2007 1515 1284 184 177 MscI TGG'CCA Cuts at: 10 64 1489 1832 4799 10 Size: 54 1425 343 2967 4452 Fragments arranged by size: 4452 2967 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4556 5585 5927 6108 7199 7558 7717 Size: 648 3390 1029 342 181 1091 359 159 Cuts at: 7717 518 Size: 2042 Fragments arranged by size: 3390 2042 1091 1029 648 359 342 181 159 MspA1I CmG'CkG Cuts at: 1606 2117 2126 2151 2188 2221 2291 2300 2361 Size: 511 9 25 37 33 70 9 61 Cuts at: 2361 2403 2423 2474 2693 3048 4105 4988 5626 Size: 42 20 51 219 355 1057 883 638 Cuts at: 5626 6657 7334 8482 8727 1606 Size: 1031 677 1148 245 2120 Fragments arranged by size: 2120 1148 1057 1031 883 677 638 511 355 245 219 70 61 51 42 37 33 25 20 9 9 NaeI GCC'GGC Cuts at: 2259 2259 Size: 9241 NarI GG'CG_CC Cuts at: 2052 2367 2393 2406 2447 2052 Size: 315 26 13 41 8846 Fragments arranged by size: 8846 315 41 26 13 NcoI C'CATG_G Cuts at: 513 1065 2004 3680 5580 6347 513 Size: 552 939 1676 1900 767 3407 Fragments arranged by size: 3407 1900 1676 939 767 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9241 NgoMIV G'CCGG_C Cuts at: 2257 2257 Size: 9241 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9241 NotI GC'GGCC_GC Cuts at: 6688 6688 Size: 9241 NspI r_CATG'y Cuts at: 1132 1229 3250 3355 3421 3909 6599 8144 1132 Size: 97 2021 105 66 488 2690 1545 2229 Fragments arranged by size: 2690 2229 2021 1545 488 105 97 66 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9241 PciI A'CATG_T Cuts at: 8140 8140 Size: 9241 PflMI CCAn_nnn'nTGG Cuts at: 1108 2326 4435 1108 Size: 1218 2109 5914 Fragments arranged by size: 5914 2109 1218 PfoI T'CCnGG_A Cuts at: 2701 2803 2701 Size: 102 9139 PmeI GTTT'AAAC Cuts at: 6545 6545 Size: 9241 PmlI CAC'GTG Cuts at: 3056 5943 3056 Size: 2887 6354 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3152 3184 4756 4788 5324 5356 6048 6080 7310 Size: 32 1572 32 536 32 692 32 1230 Cuts at: 7310 7342 8823 8855 3152 Size: 32 1481 32 3538 Fragments arranged by size: 3538 1572 1481 1230 692 536 32 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4814 5404 4814 Size: 590 8651 PshAI GACnn'nnGTC Cuts at: 1613 5831 1613 Size: 4218 5023 PsiI TTA'TAA Cuts at: 6811 6811 Size: 9241 PspOMI G'GGCC_C Cuts at: 3783 3783 Size: 9241 PspXI vC'TCGA_Gb Cuts at: 2410 2410 Size: 9241 PstI C_TGCA'G Cuts at: 830 2473 2964 3047 3395 3914 5042 5631 6181 Size: 1643 491 83 348 519 1128 589 550 Cuts at: 6181 6593 830 Size: 412 3478 Fragments arranged by size: 3478 1643 1128 589 550 519 491 412 348 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9241 PvuII CAG'CTG Cuts at: 2188 2221 2693 3048 4105 4988 5626 2188 Size: 33 472 355 1057 883 638 5803 Fragments arranged by size: 5803 1057 883 638 472 355 33 SacI G_AGCT'C Cuts at: 5388 5388 Size: 9241 SacII CC_GC'GG Cuts at: 2118 2127 2292 2362 2118 Size: 9 165 70 8997 Fragments arranged by size: 8997 165 70 9 SalI G'TCGA_C Cuts at: 1936 6583 1936 Size: 4647 4594 SapI GCTCTTCn'nnn_ Cuts at: 2243 2863 5945 6465 2243 Size: 620 3082 520 5019 Fragments arranged by size: 5019 3082 620 520 SbfI CC_TGCA'GG Cuts at: 6593 6593 Size: 9241 ScaI AGT'ACT Cuts at: 3280 4339 7489 9201 3280 Size: 1059 3150 1712 3320 Fragments arranged by size: 3320 3150 1712 1059 SfcI C'TryA_G Cuts at: 826 1045 2469 2960 3043 3391 3505 3910 4488 Size: 219 1424 491 83 348 114 405 578 Cuts at: 4488 5038 5293 5627 6177 6589 7724 8405 8596 Size: 550 255 334 550 412 1135 681 191 Cuts at: 8596 826 Size: 1471 Fragments arranged by size: 1471 1424 1135 681 578 550 550 491 412 405 348 334 255 219 191 114 83 SfoI GGC'GCC Cuts at: 2053 2368 2394 2407 2448 2053 Size: 315 26 13 41 8846 Fragments arranged by size: 8846 315 41 26 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9241 SmaI CCC'GGG Cuts at: 1872 2049 2434 6568 1872 Size: 177 385 4134 4545 Fragments arranged by size: 4545 4134 385 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 2410 3196 4465 5784 7345 Size: 197 926 153 314 786 1269 1319 1561 Cuts at: 7345 8246 8508 820 Size: 901 262 1553 Fragments arranged by size: 1561 1553 1319 1269 926 901 786 314 262 197 153 SnaBI TAC'GTA Cuts at: 493 7032 493 Size: 6539 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9241 SphI G_CATG'C Cuts at: 1132 3355 3909 6599 1132 Size: 2223 554 2690 3774 Fragments arranged by size: 3774 2690 2223 554 SspI AAT'ATT Cuts at: 5 52 7165 5 Size: 47 7113 2081 Fragments arranged by size: 7113 2081 47 StuI AGG'CCT Cuts at: 1517 5754 1517 Size: 4237 5004 StyI C'CwwG_G Cuts at: 513 1065 2004 3680 5580 5755 6347 6680 513 Size: 552 939 1676 1900 175 592 333 3074 Fragments arranged by size: 3074 1900 1676 939 592 552 333 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 7268 7285 7438 7623 8068 325 Size: 1255 5688 17 153 185 445 1498 Fragments arranged by size: 5688 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2952 3278 4337 Size: 274 80 33 51 1443 975 326 1059 Cuts at: 4337 7487 9199 96 Size: 3150 1712 138 Fragments arranged by size: 3150 1712 1443 1059 975 326 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3314 3346 3882 3914 9201 9233 3314 Size: 32 536 32 5287 32 3322 Fragments arranged by size: 5287 3322 536 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2751 3941 1316 Size: 1435 1190 6616 Fragments arranged by size: 6616 1435 1190 XbaI T'CTAG_A Cuts at: 3725 6577 3725 Size: 2852 6389 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3776 4436 4681 3776 Size: 660 245 8336 Fragments arranged by size: 8336 660 245 XhoI C'TCGA_G Cuts at: 1943 2410 1943 Size: 467 8774 XmaI C'CCGG_G Cuts at: 1870 2047 2432 6566 1870 Size: 177 385 4134 4545 Fragments arranged by size: 4545 4134 385 177 XmnI GAAnn'nnTTC Cuts at: 5047 7370 5047 Size: 2323 6918 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7049 276 Size: 53 83 186 936 5515 2468 Fragments arranged by size: 5515 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BfrBI BsiWI BstZ17I DraIII FseI FspAI NruI NsiI PacI PsrI RsrII SanDI SexAI SfiI SrfI SwaI