Restriction map for
pFN21AE3472
(Linear) (Six-base) MAPSORT of: KIEE3472.seq Check: 2656 from: 1 to: 4536
KIEE3472.orf
With 182 enzymes: SgfI *
July 13, 2017 16:34 ..
AccI GT'mk_AC
Cuts at: 0 1940 2051 4536
Size: 1940 111 2485
Fragments arranged by size:
2485 1940 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1094 2011 3001 3569 4528 4536
Size: 586 508 917 990 568 959 8
Fragments arranged by size:
990 959 917 586 568 508 8
AflIII A'CryG_T
Cuts at: 0 3937 4536
Size: 3937 599
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3247 3279 4043 4075 4536
Size: 3247 32 764 32 461
Fragments arranged by size:
3247 764 461 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3095 4536
Size: 3095 1441
ApaI G_GGCC'C
Cuts at: 0 1782 4536
Size: 1782 2754
ApaLI G'TGCA_C
Cuts at: 0 1046 2709 4536
Size: 1046 1663 1827
Fragments arranged by size:
1827 1663 1046
ApoI r'AATT_y
Cuts at: 0 1143 1248 1812 2299 2311 4536
Size: 1143 105 564 487 12 2225
Fragments arranged by size:
2225 1143 564 487 105 12
AseI AT'TA_AT
Cuts at: 0 2932 4536
Size: 2932 1604
AvaI C'yCGr_G
Cuts at: 0 42 405 427 1839 4536
Size: 42 363 22 1412 2697
Fragments arranged by size:
2697 1412 363 42 22
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2471 2504 3422 3455 4536
Size: 2471 33 918 33 1081
Fragments arranged by size:
2471 1081 918 33 33
BamHI G'GATC_C
Cuts at: 0 2179 4536
Size: 2179 2357
BanI G'GyrC_C
Cuts at: 0 46 327 361 387 400 441 4114 4248
Size: 46 281 34 26 13 41 3673 134
Cuts at: 4248 4536
Size: 288
Fragments arranged by size:
3673 288 281 134 46 41 34 26
13
BanII G_rGCy'C
Cuts at: 0 64 90 284 428 870 1782 3383 4536
Size: 64 26 194 144 442 912 1601 1153
Fragments arranged by size:
1601 1153 912 442 194 144 64 26
BbeI G_GCGC'C
Cuts at: 0 50 365 391 404 445 4536
Size: 50 315 26 13 41 4091
Fragments arranged by size:
4091 315 50 41 26 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1385 1856 2627 3722 4536
Size: 1385 471 771 1095 814
Fragments arranged by size:
1385 1095 814 771 471
BbvCI CC'TCA_GC
Cuts at: 0 3617 4536
Size: 3617 919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3968 4002 4536
Size: 3968 34 534
Fragments arranged by size:
3968 534 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3657 4536
Size: 3657 879
BclI T'GATC_A
Cuts at: 0 551 4536
Size: 551 3985
BglI GCCn_nnn'nGGC
Cuts at: 0 94 359 4183 4536
Size: 94 265 3824 353
Fragments arranged by size:
3824 353 265 94
BglII A'GATC_T
Cuts at: 0 1474 1486 4536
Size: 1474 12 3050
Fragments arranged by size:
3050 1474 12
BlpI GC'TnA_GC
Cuts at: 0 1670 2351 4536
Size: 1670 681 2185
Fragments arranged by size:
2185 1670 681
Bme1580I G_kGCm'C
Cuts at: 0 1050 1782 2713 4536
Size: 1050 732 931 1823
Fragments arranged by size:
1823 1050 931 732
BmrI ACTGGGnnnn_n'
Cuts at: 0 643 820 3124 3314 3337 4237 4536
Size: 643 177 2304 190 23 900 299
Fragments arranged by size:
2304 900 643 299 190 177 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 3402 4536
Size: 106 3296 1134
Fragments arranged by size:
3296 1134 106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 782 2023 3144 3372 4536
Size: 680 102 1241 1121 228 1164
Fragments arranged by size:
1241 1164 1121 680 228 102
Bpu10I CC'TnA_GC
Cuts at: 0 221 3617 4536
Size: 221 3396 919
Fragments arranged by size:
3396 919 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1176 2481 3764 4536
Size: 76 1100 1305 1283 772
Fragments arranged by size:
1305 1283 1100 772 76
BsaAI yAC'GTr
Cuts at: 0 1051 3938 4536
Size: 1051 2887 598
Fragments arranged by size:
2887 1051 598
BsaBI GATnn'nnATC
Cuts at: 0 3512 4536
Size: 3512 1024
BsaHI Gr'CG_yC
Cuts at: 0 47 362 388 401 442 1755 4536
Size: 47 315 26 13 41 1313 2781
Fragments arranged by size:
2781 1313 315 47 41 26 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1877 1907 4043 4073 4392 4422 4536
Size: 1877 30 2136 30 319 30 114
Fragments arranged by size:
2136 1877 319 114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 98 249 1004 2895 3044 3364 4536
Size: 98 151 755 1891 149 320 1172
Fragments arranged by size:
1891 1172 755 320 151 149 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1384 1535 2093 4223 4536
Size: 1384 151 558 2130 313
Fragments arranged by size:
2130 1384 558 313 151
BsiEI CG_ry'CG
Cuts at: 0 422 483 4536
Size: 422 61 4053
Fragments arranged by size:
4053 422 61
BsiHKAI G_wGCw'C
Cuts at: 0 1050 1807 2713 3383 4536
Size: 1050 757 906 670 1153
Fragments arranged by size:
1153 1050 906 757 670
BsmI GAATG_Cn'
Cuts at: 0 4353 4536
Size: 4353 183
BsmBI CGTCTCn'nnnn_
Cuts at: 0 464 3596 4536
Size: 464 3132 940
Fragments arranged by size:
3132 940 464
Bsp1286I G_dGCh'C
Cuts at: 0 64 90 284 428 870 1050 1782 1807
Size: 64 26 194 144 442 180 732 25
Cuts at: 1807 2713 3383 4536
Size: 906 670 1153
Fragments arranged by size:
1153 906 732 670 442 194 180 144
64 26 25
BspHI T'CATG_A
Cuts at: 0 1491 4536
Size: 1491 3045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3615 4536
Size: 3615 921
BsrBI CCG'CTC
Cuts at: 0 159 231 4536
Size: 159 72 4305
Fragments arranged by size:
4305 159 72
BsrDI GCAATG_nn'
Cuts at: 0 3587 4536
Size: 3587 949
BsrFI r'CCGG_y
Cuts at: 0 252 307 478 4536
Size: 252 55 171 4058
Fragments arranged by size:
4058 252 171 55
BssHII G'CGCG_C
Cuts at: 0 104 4536
Size: 104 4432
BssSI C'ACGA_G
Cuts at: 0 1507 4536
Size: 1507 3029
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4405 4536
Size: 4405 131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1124 2610 4536
Size: 1124 1486 1926
Fragments arranged by size:
1926 1486 1124
BstYI r'GATC_y
Cuts at: 0 664 1474 1486 2179 2453 3222 4536
Size: 664 810 12 693 274 769 1314
Fragments arranged by size:
1314 810 769 693 664 274 12
Bsu36I CC'TnA_GG
Cuts at: 0 65 902 1750 4253 4478 4536
Size: 65 837 848 2503 225 58
Fragments arranged by size:
2503 848 837 225 65 58
BtgI C'CryG_G
Cuts at: 0 110 119 284 339 354 1675 3575 4062
Size: 110 9 165 55 15 1321 1900 487
Cuts at: 4062 4342 4536
Size: 280 194
Fragments arranged by size:
1900 1321 487 280 194 165 110 55
15 9
BtsI GCAGTG_nn'
Cuts at: 0 1407 1833 2987 4536
Size: 1407 426 1154 1549
Fragments arranged by size:
1549 1407 1154 426
ClaI AT'CG_AT
Cuts at: 0 4414 4536
Size: 4414 122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1505 1540 4536
Size: 1505 35 2996
Fragments arranged by size:
2996 1505 35
DraI TTT'AAA
Cuts at: 0 1139 1964 4536
Size: 1139 825 2572
Fragments arranged by size:
2572 1139 825
DrdI GACnn_nn'nnGTC
Cuts at: 0 270 2888 4536
Size: 270 2618 1648
Fragments arranged by size:
2618 1648 270
EaeI y'GGCC_r
Cuts at: 0 419 2265 2792 4536
Size: 419 1846 527 1744
Fragments arranged by size:
1846 1744 527 419
EagI C'GGCC_G
Cuts at: 0 419 4536
Size: 419 4117
EarI CTCTTCn'nnn_
Cuts at: 0 238 858 1307 1424 3872 3940 3967 4460
Size: 238 620 449 117 2448 68 27 493
Cuts at: 4460 4536
Size: 76
Fragments arranged by size:
2448 620 493 449 238 117 76 68
27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 95 178 4536
Size: 95 83 4358
Fragments arranged by size:
4358 95 83
EcoICRI GAG'CTC
Cuts at: 0 3381 4536
Size: 3381 1155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 680 782 1094 2011 2023 3001 3144
Size: 586 94 102 312 917 12 978 143
Cuts at: 3144 3372 3569 4528 4536
Size: 228 197 959 8
Fragments arranged by size:
978 959 917 586 312 228 197 143
102 94 12 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 68 3197 4536
Size: 68 3129 1339
Fragments arranged by size:
3129 1339 68
EcoO109I rG'GnC_Cy
Cuts at: 0 625 967 1778 1779 2809 3399 4536
Size: 625 342 811 1 1030 590 1137
Fragments arranged by size:
1137 1030 811 625 590 342 1
EcoRV GAT'ATC
Cuts at: 0 3645 4536
Size: 3645 891
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 737 887 1381 2278 4536
Size: 737 150 494 897 2258
Fragments arranged by size:
2258 897 737 494 150
FspI TGC'GCA
Cuts at: 0 187 4536
Size: 187 4349
HaeII r_GCGC'y
Cuts at: 0 50 365 391 404 445 4536
Size: 50 315 26 13 41 4091
Fragments arranged by size:
4091 315 50 41 26 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 516 548 871 903 1188 1220 3234 3266
Size: 516 32 323 32 285 32 2014 32
Cuts at: 3266 3353 3385 3629 3661 3882 3914 4536
Size: 87 32 244 32 221 32 622
Fragments arranged by size:
2014 622 516 323 285 244 221 87
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 924 2638 2951 3739 4270 4536
Size: 924 1714 313 788 531 266
Fragments arranged by size:
1714 924 788 531 313 266
HindIII A'AGCT_T
Cuts at: 0 3560 4536
Size: 3560 976
HpaI GTT'AAC
Cuts at: 0 924 4536
Size: 924 3612
KasI G'GCGC_C
Cuts at: 0 46 361 387 400 441 4536
Size: 46 315 26 13 41 4095
Fragments arranged by size:
4095 315 46 41 26 13
MfeI C'AATT_G
Cuts at: 0 2247 3569 4235 4536
Size: 2247 1322 666 301
Fragments arranged by size:
2247 1322 666 301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 2099 2276 4536
Size: 584 1515 177 2260
Fragments arranged by size:
2260 1515 584 177
MscI TGG'CCA
Cuts at: 0 2794 4536
Size: 2794 1742
MslI CAynn'nnrTG
Cuts at: 0 2551 3580 3922 4103 4536
Size: 2551 1029 342 181 433
Fragments arranged by size:
2551 1029 433 342 181
MspA1I CmG'CkG
Cuts at: 0 112 121 146 183 216 286 295 356
Size: 112 9 25 37 33 70 9 61
Cuts at: 356 398 418 469 688 1043 2100 2983 3621
Size: 42 20 51 219 355 1057 883 638
Cuts at: 3621 4536
Size: 915
Fragments arranged by size:
1057 915 883 638 355 219 112 70
61 51 42 37 33 25 20 9
9
NaeI GCC'GGC
Cuts at: 0 254 4536
Size: 254 4282
NarI GG'CG_CC
Cuts at: 0 47 362 388 401 442 4536
Size: 47 315 26 13 41 4094
Fragments arranged by size:
4094 315 47 41 26 13
NcoI C'CATG_G
Cuts at: 0 1675 3575 4342 4536
Size: 1675 1900 767 194
Fragments arranged by size:
1900 1675 767 194
NgoMIV G'CCGG_C
Cuts at: 0 252 4536
Size: 252 4284
NspI r_CATG'y
Cuts at: 0 1245 1350 1416 1904 4536
Size: 1245 105 66 488 2632
Fragments arranged by size:
2632 1245 488 105 66
PflMI CCAn_nnn'nTGG
Cuts at: 0 321 2430 4536
Size: 321 2109 2106
Fragments arranged by size:
2109 2106 321
PfoI T'CCnGG_A
Cuts at: 0 696 798 4536
Size: 696 102 3738
Fragments arranged by size:
3738 696 102
PmlI CAC'GTG
Cuts at: 0 1051 3938 4536
Size: 1051 2887 598
Fragments arranged by size:
2887 1051 598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1147 1179 2751 2783 3319 3351 4043 4075
Size: 1147 32 1572 32 536 32 692 32
Cuts at: 4075 4536
Size: 461
Fragments arranged by size:
1572 1147 692 536 461 32 32 32
32
PpuMI rG'GwC_Cy
Cuts at: 0 2809 3399 4536
Size: 2809 590 1137
Fragments arranged by size:
2809 1137 590
PshAI GACnn'nnGTC
Cuts at: 0 3826 4536
Size: 3826 710
PspOMI G'GGCC_C
Cuts at: 0 1778 4536
Size: 1778 2758
PspXI vC'TCGA_Gb
Cuts at: 0 405 4536
Size: 405 4131
PstI C_TGCA'G
Cuts at: 0 468 959 1042 1390 1909 3037 3626 4176
Size: 468 491 83 348 519 1128 589 550
Cuts at: 4176 4536
Size: 360
Fragments arranged by size:
1128 589 550 519 491 468 360 348
83
PvuII CAG'CTG
Cuts at: 0 183 216 688 1043 2100 2983 3621 4536
Size: 183 33 472 355 1057 883 638 915
Fragments arranged by size:
1057 915 883 638 472 355 183 33
SacI G_AGCT'C
Cuts at: 0 3383 4536
Size: 3383 1153
SacII CC_GC'GG
Cuts at: 0 113 122 287 357 4536
Size: 113 9 165 70 4179
Fragments arranged by size:
4179 165 113 70 9
SapI GCTCTTCn'nnn_
Cuts at: 0 238 858 3940 4460 4536
Size: 238 620 3082 520 76
Fragments arranged by size:
3082 620 520 238 76
ScaI AGT'ACT
Cuts at: 0 1275 2334 4536
Size: 1275 1059 2202
Fragments arranged by size:
2202 1275 1059
SfcI C'TryA_G
Cuts at: 0 464 955 1038 1386 1500 1905 2483 3033
Size: 464 491 83 348 114 405 578 550
Cuts at: 3033 3288 3622 4172 4536
Size: 255 334 550 364
Fragments arranged by size:
578 550 550 491 464 405 364 348
334 255 114 83
SfoI GGC'GCC
Cuts at: 0 48 363 389 402 443 4536
Size: 48 315 26 13 41 4093
Fragments arranged by size:
4093 315 48 41 26 13
SmaI CCC'GGG
Cuts at: 0 44 429 4536
Size: 44 385 4107
Fragments arranged by size:
4107 385 44
SmlI C'TyrA_G
Cuts at: 0 91 405 1191 2460 3779 4536
Size: 91 314 786 1269 1319 757
Fragments arranged by size:
1319 1269 786 757 314 91
SphI G_CATG'C
Cuts at: 0 1350 1904 4536
Size: 1350 554 2632
Fragments arranged by size:
2632 1350 554
StuI AGG'CCT
Cuts at: 0 3749 4536
Size: 3749 787
StyI C'CwwG_G
Cuts at: 0 1675 3575 3750 4342 4536
Size: 1675 1900 175 592 194
Fragments arranged by size:
1900 1675 592 194 175
TatI w'GTAC_w
Cuts at: 0 947 1273 2332 4536
Size: 947 326 1059 2204
Fragments arranged by size:
2204 1059 947 326
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1309 1341 1877 1909 4536
Size: 1309 32 536 32 2627
Fragments arranged by size:
2627 1309 536 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 746 1936 4536
Size: 746 1190 2600
Fragments arranged by size:
2600 1190 746
XbaI T'CTAG_A
Cuts at: 0 1720 4536
Size: 1720 2816
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1771 2431 2676 4536
Size: 1771 660 245 1860
Fragments arranged by size:
1860 1771 660 245
XhoI C'TCGA_G
Cuts at: 0 405 4536
Size: 405 4131
XmaI C'CCGG_G
Cuts at: 0 42 427 4536
Size: 42 385 4109
Fragments arranged by size:
4109 385 42
XmnI GAAnn'nnTTC
Cuts at: 0 3042 4536
Size: 3042 1494
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApaI ApaLI ApoI
AseI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII
SapI ScaI SfcI SfoI SmaI SmlI SphI StuI
StyI TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AscI AsiSI AvrII BfrBI BmgBI BmtI
BsaI BsaWI BseYI BsiWI BspEI BsrGI BstBI BstEII
BstZ17I BtgZI DraIII EcoRI FseI FspAI KpnI MluI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PsiI PsrI PvuI RsrII SalI SanDI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI TaqII ZraI