################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BalI BamHI BbeI BbrPI BbvCI BcgI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MunI NarI NdeI NheI NotI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 2272 CGCG CG^CG AcyI 363 GGCGTC G[GA]^CG[CT]C AflII 321 CTTAAG C^TTAAG AflIII 1215 ACATGT A^C[GA][CT]GT AocI 760 CCTCAGG CC^T[ACGT]AGG ApaI 658 GGGCCC GGGCC^C Asp700I 627 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1126 GAGCTC G[AT]GC[AT]^C AvaI 965 CCCGGG C^[CT]CG[GA]G AvrII 289 CCTAGG C^CTAGG BanI 698 GGCACC G^G[CT][GA]CC BclI 881 TGATCA T^GATCA BfrI 321 CTTAAG C^TTAAG BglI 726 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1013 AGATCT A^GATCT BlnI 289 CCTAGG C^CTAGG BsaBI 1300 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 363 GGCGTC G[GA]^CG[CT]C BsaI 193 GGTCTC GGTCTC(1/5) BsiHKAI 1126 GAGCTC G[AT]GC[AT]^C BsoBI 965 CCCGGG C^[CT]CG[GA]G BspLU11I 1215 ACATGT A^CATGT BspMI 1027 ACCTGC ACCTGC(4/8) BsrBI 55 GAGCGG CCGCTC(-3/-3) BstUI 2272 CGCG CG^CG Bsu36I 760 CCTCAGG CC^T[ACGT]AGG EaeI 1083 CGGCCA [CT]^GGCC[GA] Eco81I 760 CCTCAGG CC^T[ACGT]AGG EcoT22I 566 ATGCAT ATGCA^T FbaI 881 TGATCA T^GATCA HgaI 364 GCGTC GACGC(5/10) Hin1I 363 GGCGTC G[GA]^CG[CT]C HincII 1211 GTCAAC GT[CT]^[GA]AC HindII 1211 GTCAAC GT[CT]^[GA]AC MamI 1300 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 2272 CGCG CG^CG NruI 2271 TCGCGA TCG^CGA NsiI 566 ATGCAT ATGCA^T NspI 1215 ACATGT [GA]CATG^[CT] PflMI 1086 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 642 CAGCTG CAG^CTG SacI 1126 GAGCTC GAGCT^C SapI 976 GAAGAGC GCTCTTC(1/4) SexAI 986 ACCTGGT A^CC[AT]GGT SmaI 965 CCCGGG CCC^GGG TaqI 207 TCGA T^CGA TthHB8I 207 TCGA T^CGA Van91I 1086 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1439 CCAAAGGACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 965 CCCGGG C^CCGGG XmaI 965 CCCGGG C^CCGGG XmnI 627 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 411 AAATTT [GA]^AATT[CT] 1201 AAATTT AfaI 476 GTAC GT^AC 1002 GTAC AlwNI 982 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 1087 CAGGAACTG ApoI 411 AAATTT [GA]^AATT[CT] 1201 AAATTT BbsI 37 GAAGAC GAAGAC(2/6) 844 GAAGAC BciVI 868 GGATAC GTATCC(6/5) 1715 GGATAC BcnI 965 CCCGG CC^[GC]GG 966 CCGGG BpmI 247 CTCCAG CTGGAG(16/14) 464 CTGGAG BpuAI 37 GAAGAC GAAGAC(2/6) 844 GAAGAC BsgI 811 CTGCAC GTGCAG(16/14) 683 GTGCAG BsmI 353 GAATGC GAATGC(1/-1) 420 GAATGC BsrFI 1853 GCCGGC [GA]^CCGG[CT] 1937 GCCGGC Cfr10I 1853 GCCGGC [GA]^CCGG[CT] 1937 GCCGGC DraII 658 GGGCCCC [GA]G^G[ACGT]CC[CT] 1420 AGGGCCT DsaI 65 CCATGG C^C[GA][CT]GG 654 CCATGG EcoO109I 658 GGGCCCC [GA]G^G[ACGT]CC[CT] 1420 AGGGCCT MseI 322 TTAA T^TAA 1604 TTAA MspA1I 642 CAGCTG C[AC]G^C[GT]G 802 CCGCTG MwoI 726 GCCCCCAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 804 GCTGGGTCTGC NaeI 1853 GCCGGC GCC^GGC 1937 GCCGGC NciI 965 CCCGG CC^[GC]GG 966 CCGGG NcoI 65 CCATGG C^CATGG 654 CCATGG NgoMIV 1853 GCCGGC G^CCGGC 1937 GCCGGC PleI 1034 GAGTC GAGTC(4/5) 1489 GAGTC RsaI 476 GTAC GT^AC 1002 GTAC Tru9I 322 TTAA T^TAA 1604 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BfaI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspI MvaI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI TseI Tsp45I Tsp509I TspRI XhoII