(Circular) (Six-base) MAPSORT of: pFN21AE2137.seq Check: 5959 from: 1 to: 7387 pFN21AE2137 7387 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5197 278 Size: 53 83 186 936 3661 2468 Fragments arranged by size: 3661 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4730 1937 Size: 2793 4594 Acc65I G'GTAC_C Cuts at: 4708 4708 Size: 7387 AclI AA'CG_TT Cuts at: 1563 5514 5887 1563 Size: 3951 373 3063 Fragments arranged by size: 3951 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5450 6834 7102 1632 Size: 3818 1384 268 1917 Fragments arranged by size: 3818 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2326 820 Size: 197 1309 5881 Fragments arranged by size: 5881 1309 197 AflIII A'CryG_T Cuts at: 1203 3220 6286 7263 1203 Size: 2017 3066 977 1327 Fragments arranged by size: 3066 2017 1327 977 AgeI A'CCGG_T Cuts at: 6218 6218 Size: 7387 AhdI GACnn_n'nnGTC Cuts at: 6116 6116 Size: 7387 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7387 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7387 AlwNI CAG_nnn'CTG Cuts at: 2992 3097 5130 6702 2992 Size: 105 2033 1572 3677 Fragments arranged by size: 3677 2033 1572 105 ApaI G_GGCC'C Cuts at: 2667 2667 Size: 7387 ApaLI G'TGCA_C Cuts at: 5444 6600 5444 Size: 1156 6231 ApoI r'AATT_y Cuts at: 1493 2416 3206 4696 4922 5074 7183 1493 Size: 923 790 1490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 923 790 226 152 AseI AT'TA_AT Cuts at: 160 1032 5941 160 Size: 872 4909 1606 Fragments arranged by size: 4909 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7387 AvaI C'yCGr_G Cuts at: 1870 1943 2970 4712 1870 Size: 73 1027 1742 4545 Fragments arranged by size: 4545 1742 1027 73 AvrII C'CTAG_G Cuts at: 2294 2294 Size: 7387 BamHI G'GATC_C Cuts at: 4717 5084 4717 Size: 367 7020 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2703 4708 6163 618 Size: 325 217 627 916 2005 1455 1842 Fragments arranged by size: 2005 1842 1455 916 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2036 2667 3135 4706 1391 Size: 621 24 631 468 1571 4072 Fragments arranged by size: 4072 1571 631 621 468 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2049 2856 928 Size: 971 150 807 5459 Fragments arranged by size: 5459 971 807 150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5578 5612 1903 Size: 34 3641 34 3678 Fragments arranged by size: 3678 3641 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2867 3714 5279 6489 2867 Size: 847 1565 1210 3765 Fragments arranged by size: 3765 1565 1210 847 BclI T'GATC_A Cuts at: 1548 2886 1548 Size: 1338 6049 BfrBI ATG'CAT Cuts at: 2573 2573 Size: 7387 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2737 5998 136 Size: 107 122 71 631 605 1065 3261 1525 Fragments arranged by size: 3261 1525 1065 631 605 122 107 71 BglII A'GATC_T Cuts at: 3018 7382 3018 Size: 4364 3023 BlpI GC'TnA_GC Cuts at: 4804 4804 Size: 7387 Bme1580I G_kGCm'C Cuts at: 1790 2667 2706 2971 5448 6604 1790 Size: 877 39 265 2477 1156 2573 Fragments arranged by size: 2573 2477 1156 877 265 39 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7387 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2149 3107 5118 Size: 416 53 83 399 24 732 958 2011 Cuts at: 5118 6076 442 Size: 958 1753 Fragments arranged by size: 2011 1753 958 958 732 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7387 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 2490 6047 1239 Size: 998 253 3557 2579 Fragments arranged by size: 3557 2579 998 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2482 3544 5512 6377 6675 2482 Size: 1062 1968 865 298 3194 Fragments arranged by size: 3194 1968 1062 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2204 6050 882 Size: 617 705 3846 2219 Fragments arranged by size: 3846 2219 705 617 BsaAI yAC'GTr Cuts at: 493 1204 5178 493 Size: 711 3974 2702 Fragments arranged by size: 3974 2702 711 BsaBI GATnn'nnATC Cuts at: 3309 7381 3309 Size: 4072 3315 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2369 5194 5576 6211 Size: 53 83 186 936 836 2825 382 635 Cuts at: 6211 275 Size: 1451 Fragments arranged by size: 2825 1451 936 836 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5820 6218 6492 6639 7050 5820 Size: 398 274 147 411 6157 Fragments arranged by size: 6157 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7162 Fragments arranged by size: 7162 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 3229 3266 3695 3779 3863 3901 3947 Size: 856 1187 37 429 84 84 38 46 Cuts at: 3947 3985 4031 4115 4199 4283 4367 4451 4535 Size: 38 46 84 84 84 84 84 84 Cuts at: 4535 1186 Size: 4038 Fragments arranged by size: 4038 1187 856 429 84 84 84 84 84 84 84 84 46 46 38 38 37 BseYI C'CCAG_C Cuts at: 2771 2809 6590 7086 2771 Size: 38 3781 496 3072 Fragments arranged by size: 3781 3072 496 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2709 2801 1163 Size: 573 973 92 5749 Fragments arranged by size: 5749 973 573 92 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4837 5598 6626 1540 Size: 275 186 2836 761 1028 2301 Fragments arranged by size: 2836 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3135 5448 5533 6604 3135 Size: 2313 85 1071 3918 Fragments arranged by size: 3918 2313 1071 85 BsmI GAATG_Cn' Cuts at: 2364 2431 4898 4991 2364 Size: 67 2467 93 4760 Fragments arranged by size: 4760 2467 93 67 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2036 2667 2706 2971 3135 4706 Size: 399 222 24 631 39 265 164 1571 Cuts at: 4706 5448 5533 6604 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1571 1071 742 631 399 265 222 164 85 39 24 BspEI T'CCGG_A Cuts at: 7050 7050 Size: 7387 BspHI T'CATG_A Cuts at: 5276 7006 5276 Size: 1730 5657 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3041 4742 844 Size: 2197 1701 3489 Fragments arranged by size: 3489 2197 1701 BsrBI CCG'CTC Cuts at: 1394 1823 2062 5274 1394 Size: 429 239 3212 3507 Fragments arranged by size: 3507 3212 429 239 BsrDI GCAATG_nn' Cuts at: 65 1248 5882 6056 65 Size: 1183 4634 174 1396 Fragments arranged by size: 4634 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3858 3942 6031 6218 1700 Size: 110 2048 84 2089 187 2869 Fragments arranged by size: 2869 2089 2048 187 110 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7387 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7387 BssSI C'ACGA_G Cuts at: 5447 6459 5447 Size: 1012 6375 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7387 BstBI TT'CG_AA Cuts at: 6191 6191 Size: 7387 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7387 BstYI r'GATC_y Cuts at: 1972 3018 3162 3331 4717 5084 5469 5486 6938 Size: 1046 144 169 1386 367 385 17 1452 Cuts at: 6938 7024 7036 7382 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1386 1046 385 367 346 169 144 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2766 2766 Size: 7387 BtgI C'CryG_G Cuts at: 513 1065 2070 2659 513 Size: 552 1005 589 5241 Fragments arranged by size: 5241 1005 589 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6648 Fragments arranged by size: 6648 683 56 BtsI GCAGTG_nn' Cuts at: 2697 4903 5697 5717 2697 Size: 2206 794 20 4367 Fragments arranged by size: 4367 2206 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7387 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7352 DraI TTT'AAA Cuts at: 4691 4691 Size: 7387 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6394 809 Size: 507 5078 1802 Fragments arranged by size: 5078 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3088 4834 5723 Size: 54 1425 50 275 18 1258 1746 889 Cuts at: 5723 7352 8 Size: 1629 43 Fragments arranged by size: 1746 1629 1425 1258 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4834 1537 Size: 275 3022 4090 Fragments arranged by size: 4090 3022 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2850 2976 3486 5317 1353 Size: 1497 126 510 1831 3423 Fragments arranged by size: 3423 1831 1497 510 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5974 6348 6494 387 Size: 1064 138 4385 374 146 1280 Fragments arranged by size: 4385 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3133 3133 Size: 7387 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2490 5450 6047 6834 7102 1239 Size: 393 605 253 2960 597 787 268 1524 Fragments arranged by size: 2960 1524 787 605 597 393 268 253 EcoO109I rG'GnC_Cy Cuts at: 2664 3426 2664 Size: 762 6625 EcoRI G'AATT_C Cuts at: 4696 5074 4696 Size: 378 7009 FspI TGC'GCA Cuts at: 5893 5893 Size: 7387 HaeII r_GCGC'y Cuts at: 1678 6534 1678 Size: 4856 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 6028 6060 6102 6134 1373 Size: 32 135 32 4456 32 42 32 2626 Fragments arranged by size: 4456 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3218 4731 4977 669 Size: 988 281 1280 1513 246 3079 Fragments arranged by size: 3079 1513 1280 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7387 HpaI GTT'AAC Cuts at: 4977 4977 Size: 7387 KpnI G_GTAC'C Cuts at: 4712 4712 Size: 7387 MfeI C'AATT_G Cuts at: 4986 4986 Size: 7387 MluI A'CGCG_T Cuts at: 7263 7263 Size: 7387 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3304 6501 6685 1305 Size: 1999 3197 184 2007 Fragments arranged by size: 3197 2007 1999 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5565 Fragments arranged by size: 5565 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5345 5704 5863 518 Size: 648 4179 359 159 2042 Fragments arranged by size: 4179 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2649 2809 4803 5480 6628 6873 1606 Size: 1043 160 1994 677 1148 245 2120 Fragments arranged by size: 2120 1994 1148 1043 677 245 160 NaeI GCC'GGC Cuts at: 3860 3944 3860 Size: 84 7303 NcoI C'CATG_G Cuts at: 513 1065 2070 2659 513 Size: 552 1005 589 5241 Fragments arranged by size: 5241 1005 589 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7387 NgoMIV G'CCGG_C Cuts at: 3858 3942 3858 Size: 84 7303 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7387 NotI GC'GGCC_GC Cuts at: 4834 4834 Size: 7387 NruI TCG'CGA Cuts at: 4278 4278 Size: 7387 NsiI A_TGCA'T Cuts at: 2575 2575 Size: 7387 NspI r_CATG'y Cuts at: 1132 1229 3224 4745 6290 1132 Size: 97 1995 1521 1545 2229 Fragments arranged by size: 2229 1995 1545 1521 97 PasI CC'CwG_GG Cuts at: 1348 2735 3299 1348 Size: 1387 564 5436 Fragments arranged by size: 5436 1387 564 PciI A'CATG_T Cuts at: 3220 6286 3220 Size: 3066 4321 PflMI CCAn_nnn'nTGG Cuts at: 1108 3097 1108 Size: 1989 5398 PmeI GTTT'AAAC Cuts at: 4691 4691 Size: 7387 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5456 5488 6969 7001 5456 Size: 32 1481 32 5842 Fragments arranged by size: 5842 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7387 PsiI TTA'TAA Cuts at: 4957 4957 Size: 7387 PspOMI G'GGCC_C Cuts at: 2663 2663 Size: 7387 PstI C_TGCA'G Cuts at: 830 3668 3752 3836 3920 4004 4088 4172 4256 Size: 2838 84 84 84 84 84 84 84 Cuts at: 4256 4340 4424 4508 4592 4676 4739 830 Size: 84 84 84 84 84 63 3478 Fragments arranged by size: 3478 2838 84 84 84 84 84 84 84 84 84 84 84 84 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7387 PvuII CAG'CTG Cuts at: 2649 2649 Size: 7387 SacI G_AGCT'C Cuts at: 3135 3135 Size: 7387 SalI G'TCGA_C Cuts at: 1936 4729 1936 Size: 2793 4594 SapI GCTCTTCn'nnn_ Cuts at: 2976 2976 Size: 7387 SbfI CC_TGCA'GG Cuts at: 4739 4739 Size: 7387 ScaI AGT'ACT Cuts at: 5635 7347 5635 Size: 1712 5675 SexAI A'CCwGG_T Cuts at: 2991 2991 Size: 7387 SfcI C'TryA_G Cuts at: 826 1045 2155 2193 3025 3463 3664 3748 3832 Size: 219 1110 38 832 438 201 84 84 Cuts at: 3832 3916 4000 4084 4168 4252 4336 4420 4504 Size: 84 84 84 84 84 84 84 84 Cuts at: 4504 4588 4672 4735 5870 6551 6742 826 Size: 84 84 63 1135 681 191 1471 Fragments arranged by size: 1471 1135 1110 832 681 438 219 201 191 84 84 84 84 84 84 84 84 84 84 84 84 63 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7387 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7387 SmaI CCC'GGG Cuts at: 1872 2972 4714 1872 Size: 1100 1742 4545 Fragments arranged by size: 4545 1742 1100 SmlI C'TyrA_G Cuts at: 820 1017 1943 2326 2497 3559 5491 6392 6654 Size: 197 926 383 171 1062 1932 901 262 Cuts at: 6654 820 Size: 1553 Fragments arranged by size: 1932 1553 1062 926 901 383 262 197 171 SnaBI TAC'GTA Cuts at: 493 5178 493 Size: 4685 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7387 SphI G_CATG'C Cuts at: 1132 4745 1132 Size: 3613 3774 SspI AAT'ATT Cuts at: 5 52 5311 5 Size: 47 5259 2081 Fragments arranged by size: 5259 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7387 StyI C'CwwG_G Cuts at: 513 1065 2070 2112 2294 2315 2364 2659 2892 Size: 552 1005 42 182 21 49 295 233 Cuts at: 2892 4826 513 Size: 1934 3074 Fragments arranged by size: 3074 1934 1005 552 295 233 182 49 42 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3457 5414 5431 5584 5769 6214 325 Size: 1255 1877 1957 17 153 185 445 1498 Fragments arranged by size: 1957 1877 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5633 7345 96 Size: 274 80 33 51 1443 3656 1712 138 Fragments arranged by size: 3656 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7347 7379 7347 Size: 32 7355 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7387 XbaI T'CTAG_A Cuts at: 4723 4723 Size: 7387 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3451 3451 Size: 7387 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7387 XmaI C'CCGG_G Cuts at: 1870 2970 4712 1870 Size: 1100 1742 4545 Fragments arranged by size: 4545 1742 1100 XmnI GAAnn'nnTTC Cuts at: 2636 5516 2636 Size: 2880 4507 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5195 276 Size: 53 83 186 936 3661 2468 Fragments arranged by size: 3661 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI