Restriction map for
pFN21AE2137
(Linear) (Six-base) MAPSORT of: KIEE2137.seq Check: 8466 from: 1 to: 2682
KIEE2137.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AflII C'TTAA_G
Cuts at: 0 321 2682
Size: 321 2361
AflIII A'CryG_T
Cuts at: 0 1215 2682
Size: 1215 1467
AlwNI CAG_nnn'CTG
Cuts at: 0 987 1092 2682
Size: 987 105 1590
Fragments arranged by size:
1590 987 105
ApaI G_GGCC'C
Cuts at: 0 662 2682
Size: 662 2020
ApoI r'AATT_y
Cuts at: 0 411 1201 2682
Size: 411 790 1481
Fragments arranged by size:
1481 790 411
AvaI C'yCGr_G
Cuts at: 0 965 2682
Size: 965 1717
AvrII C'CTAG_G
Cuts at: 0 289 2682
Size: 289 2393
BanI G'GyrC_C
Cuts at: 0 698 2682
Size: 698 1984
BanII G_rGCy'C
Cuts at: 0 7 31 662 1130 2682
Size: 7 24 631 468 1552
Fragments arranged by size:
1552 631 468 24 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 44 851 2682
Size: 44 807 1831
Fragments arranged by size:
1831 807 44
BciVI GTATCCnnnnn_n'
Cuts at: 0 862 1709 2682
Size: 862 847 973
Fragments arranged by size:
973 862 847
BclI T'GATC_A
Cuts at: 0 881 2682
Size: 881 1801
BfrBI ATG'CAT
Cuts at: 0 568 2682
Size: 568 2114
BglI GCCn_nnn'nGGC
Cuts at: 0 732 2682
Size: 732 1950
BglII A'GATC_T
Cuts at: 0 1013 2682
Size: 1013 1669
Bme1580I G_kGCm'C
Cuts at: 0 662 701 966 2682
Size: 662 39 265 1716
Fragments arranged by size:
1716 662 265 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 1102 2682
Size: 144 958 1580
Fragments arranged by size:
1580 958 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 485 2682
Size: 232 253 2197
Fragments arranged by size:
2197 253 232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 1539 2682
Size: 477 1062 1143
Fragments arranged by size:
1143 1062 477
BsaI GGTCTCn'nnnn_
Cuts at: 0 199 2682
Size: 199 2483
BsaBI GATnn'nnATC
Cuts at: 0 1304 2682
Size: 1304 1378
BsaHI Gr'CG_yC
Cuts at: 0 364 2682
Size: 364 2318
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 1224 1261 1690 1774 1858 1896 1942
Size: 37 1187 37 429 84 84 38 46
Cuts at: 1942 1980 2026 2110 2194 2278 2362 2446 2530
Size: 38 46 84 84 84 84 84 84
Cuts at: 2530 2682
Size: 152
Fragments arranged by size:
1187 429 152 84 84 84 84 84
84 84 84 46 46 38 38 37
37
BseYI C'CCAG_C
Cuts at: 0 766 804 2682
Size: 766 38 1878
Fragments arranged by size:
1878 766 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 796 2682
Size: 704 92 1886
Fragments arranged by size:
1886 704 92
BsiHKAI G_wGCw'C
Cuts at: 0 1130 2682
Size: 1130 1552
BsmI GAATG_Cn'
Cuts at: 0 359 426 2682
Size: 359 67 2256
Fragments arranged by size:
2256 359 67
Bsp1286I G_dGCh'C
Cuts at: 0 7 31 662 701 966 1130 2682
Size: 7 24 631 39 265 164 1552
Fragments arranged by size:
1552 631 265 164 39 24 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1036 2682
Size: 1036 1646
BsrBI CCG'CTC
Cuts at: 0 57 2682
Size: 57 2625
BsrFI r'CCGG_y
Cuts at: 0 1853 1937 2682
Size: 1853 84 745
Fragments arranged by size:
1853 745 84
BstYI r'GATC_y
Cuts at: 0 1013 1157 1326 2682
Size: 1013 144 169 1356
Fragments arranged by size:
1356 1013 169 144
Bsu36I CC'TnA_GG
Cuts at: 0 761 2682
Size: 761 1921
BtgI C'CryG_G
Cuts at: 0 65 654 2682
Size: 65 589 2028
Fragments arranged by size:
2028 589 65
BtsI GCAGTG_nn'
Cuts at: 0 692 2682
Size: 692 1990
EaeI y'GGCC_r
Cuts at: 0 1083 2682
Size: 1083 1599
EarI CTCTTCn'nnn_
Cuts at: 0 845 971 1481 2682
Size: 845 126 510 1201
Fragments arranged by size:
1201 845 510 126
EcoICRI GAG'CTC
Cuts at: 0 1128 2682
Size: 1128 1554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 485 2682
Size: 232 253 2197
Fragments arranged by size:
2197 253 232
EcoO109I rG'GnC_Cy
Cuts at: 0 659 1421 2682
Size: 659 762 1261
Fragments arranged by size:
1261 762 659
HincII GTy'rAC
Cuts at: 0 1213 2682
Size: 1213 1469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1299 2682
Size: 1299 1383
MspA1I CmG'CkG
Cuts at: 0 644 804 2682
Size: 644 160 1878
Fragments arranged by size:
1878 644 160
NaeI GCC'GGC
Cuts at: 0 1855 1939 2682
Size: 1855 84 743
Fragments arranged by size:
1855 743 84
NcoI C'CATG_G
Cuts at: 0 65 654 2682
Size: 65 589 2028
Fragments arranged by size:
2028 589 65
NgoMIV G'CCGG_C
Cuts at: 0 1853 1937 2682
Size: 1853 84 745
Fragments arranged by size:
1853 745 84
NruI TCG'CGA
Cuts at: 0 2273 2682
Size: 2273 409
NsiI A_TGCA'T
Cuts at: 0 570 2682
Size: 570 2112
NspI r_CATG'y
Cuts at: 0 1219 2682
Size: 1219 1463
PasI CC'CwG_GG
Cuts at: 0 730 1294 2682
Size: 730 564 1388
Fragments arranged by size:
1388 730 564
PciI A'CATG_T
Cuts at: 0 1215 2682
Size: 1215 1467
PflMI CCAn_nnn'nTGG
Cuts at: 0 1092 2682
Size: 1092 1590
PspOMI G'GGCC_C
Cuts at: 0 658 2682
Size: 658 2024
PstI C_TGCA'G
Cuts at: 0 1663 1747 1831 1915 1999 2083 2167 2251
Size: 1663 84 84 84 84 84 84 84
Cuts at: 2251 2335 2419 2503 2587 2671 2682
Size: 84 84 84 84 84 11
Fragments arranged by size:
1663 84 84 84 84 84 84 84
84 84 84 84 84 11
PvuII CAG'CTG
Cuts at: 0 644 2682
Size: 644 2038
SacI G_AGCT'C
Cuts at: 0 1130 2682
Size: 1130 1552
SapI GCTCTTCn'nnn_
Cuts at: 0 971 2682
Size: 971 1711
SexAI A'CCwGG_T
Cuts at: 0 986 2682
Size: 986 1696
SfcI C'TryA_G
Cuts at: 0 150 188 1020 1458 1659 1743 1827 1911
Size: 150 38 832 438 201 84 84 84
Cuts at: 1911 1995 2079 2163 2247 2331 2415 2499 2583
Size: 84 84 84 84 84 84 84 84
Cuts at: 2583 2667 2682
Size: 84 15
Fragments arranged by size:
832 438 201 150 84 84 84 84
84 84 84 84 84 84 84 84
38 15
SmaI CCC'GGG
Cuts at: 0 967 2682
Size: 967 1715
SmlI C'TyrA_G
Cuts at: 0 321 492 1554 2682
Size: 321 171 1062 1128
Fragments arranged by size:
1128 1062 321 171
StyI C'CwwG_G
Cuts at: 0 65 107 289 310 359 654 887 2682
Size: 65 42 182 21 49 295 233 1795
Fragments arranged by size:
1795 295 233 182 65 49 42 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1452 2682
Size: 1452 1230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1446 2682
Size: 1446 1236
XmaI C'CCGG_G
Cuts at: 0 965 2682
Size: 965 1717
XmnI GAAnn'nnTTC
Cuts at: 0 631 2682
Size: 631 2051
Enzymes that do cut:
AflII AflIII AlwNI ApaI ApoI AvaI AvrII BanI
BanII BbsI BciVI BclI BfrBI BglI BglII Bme1580I
BmrI BpmI BpuEI BsaI BsaBI BsaHI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstYI
Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
HincII MmeI MspA1I NaeI NcoI NgoMIV NruI NsiI
NspI PasI PciI PflMI PspOMI PstI PvuII SacI
SapI SexAI SfcI SmaI SmlI StyI TaqII XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
BaeI BamHI BbeI BbvCI BcgI BlpI BmgBI BmtI
BplI Bpu10I BsaAI BsaWI BsaXI BsiEI BsiWI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI MscI MslI NarI NdeI NheI NotI
PacI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI ZraI