################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspHI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspLU11I BsrFI BssHII BssSI BstUI BstXI Bsu36I CelII CfoI Cfr10I CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SmlI SpeI Sse8387I StuI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AclI 1402 AACGTT AA^CGTT ApaI 2053 GGGCCC GGGCC^C AseI 1009 ATTAAT AT^TAAT AsnI 1009 ATTAAT AT^TAAT AspI 622 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 480 CTCGGG C^[CT]CG[GA]G BanI 253 GGTGCC G^G[CT][GA]CC BanII 2053 GGGCCC G[GA]GC[CT]^C BcgI 1622 GCAGCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1863 AGATCT A^GATCT BmyI 2053 GGGCCC G[AGT]GC[ACT]^C BsmAI 1724 GTCTC GTCTC(1/5) BsmFI 2034 GGGAC GGGAC(10/14) BsoBI 480 CTCGGG C^[CT]CG[GA]G Bsp1286I 2053 GGGCCC G[AGT]GC[ACT]^C BspDI 637 ATCGAT AT^CGAT BspMI 245 ACCTGC ACCTGC(4/8) BsrBI 2042 GAGCGG CCGCTC(-3/-3) Bst1107I 1048 GTATAC GTA^TAC BstBI 1268 TTCGAA TT^CGAA BstEII 664 GGTTACC G^GT[ACGT]ACC BstPI 664 GGTTACC G^GT[ACGT]ACC BstZ17I 1048 GTATAC GTA^TAC ClaI 637 ATCGAT AT^CGAT DraI 736 TTTAAA TTT^AAA DraII 2034 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 2034 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 664 GGTTACC G^GT[ACGT]ACC EcoRV 1531 GATATC GAT^ATC EcoT14I 1979 CCAAGG C^C[AT][AT]GG MslI 934 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspV 1268 TTCGAA TT^CGAA PflFI 622 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2034 GGGACCT [GA]G^G[AT]CC[CT] PshBI 1009 ATTAAT AT^TAAT Psp1406I 1402 AACGTT AA^CGTT SapI 336 GAAGAGC GCTCTTC(1/4) SexAI 1915 ACCAGGT A^CC[AT]GGT SfcI 1497 CTACAG C^T[GA][CT]AG SfuI 1268 TTCGAA TT^CGAA SphI 112 GCATGC GCATG^C StyI 1979 CCAAGG C^C[AT][AT]GG Tth111I 622 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1772 CCAAAACTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 2002 CCGC CCGC(-3/-1) 2044 GCGG AlwNI 241 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 1957 CAGAAACTG BpmI 1505 CTGGAG CTGGAG(16/14) 2039 CTGGAG BsaAI 613 TACGTA [CT]AC^GT[GA] 1045 TACGTA BsaJI 479 CCTCGG C^C[ACGT][ACGT]GG 1979 CCAAGG BseRI 421 GAGGAG GAGGAG(10/8) 448 GAGGAG BsiYI 1018 CCTGAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1926 CCGAATCTTGG BslI 1018 CCTGAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1926 CCGAATCTTGG Bsp1407I 530 TGTACA T^GTACA 1547 TGTACA BsrDI 258 CATTGC GCAATG(2/0) 1836 CATTGC BsrGI 530 TGTACA T^GTACA 1547 TGTACA BstF5I 98 GGATG GGATG(2/0) 132 GGATG BstYI 1827 GGATCT [GA]^GATC[CT] 1863 AGATCT EaeI 1026 TGGCCG [CT]^GGCC[GA] 1685 CGGCCA EcoT22I 941 ATGCAT ATGCA^T 1261 ATGCAT FokI 98 GGATG GGATG(9/13) 132 GGATG HphI 1090 GGTGA GGTGA(8/7) 1633 GGTGA MflI 1827 GGATCT [GA]^GATC[CT] 1863 AGATCT NsiI 941 ATGCAT ATGCA^T 1261 ATGCAT SnaBI 613 TACGTA TAC^GTA 1045 TACGTA SspBI 530 TGTACA T^GTACA 1547 TGTACA XhoII 1827 GGATCT [GA]^GATC[CT] 1863 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccI AcsI AfaI AluI AlwI ApoI AvaII BbvI BfaI BsmI BsrI BssKI BstAPI BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI HaeIII HinfI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I