(Circular) (Six-base) MAPSORT of: pFN21AE5721.seq Check: 1135 from: 1 to: 6775 pFN21AE5721 6775 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4585 278 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3003 3054 3071 3501 3826 3906 4118 1937 Size: 1066 51 17 430 325 80 212 4594 Fragments arranged by size: 4594 1066 430 325 212 80 51 17 Acc65I G'GTAC_C Cuts at: 4096 4096 Size: 6775 AclI AA'CG_TT Cuts at: 1563 3408 4902 5275 1563 Size: 1845 1494 373 3063 Fragments arranged by size: 3063 1845 1494 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2181 2881 4838 6222 6490 1632 Size: 549 700 1957 1384 268 1917 Fragments arranged by size: 1957 1917 1384 700 549 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6578 AflIII A'CryG_T Cuts at: 1203 5674 6651 1203 Size: 4471 977 1327 Fragments arranged by size: 4471 1327 977 AgeI A'CCGG_T Cuts at: 5606 5606 Size: 6775 AhdI GACnn_n'nnGTC Cuts at: 5504 5504 Size: 6775 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6775 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6775 AlwNI CAG_nnn'CTG Cuts at: 2251 3967 4518 6090 2251 Size: 1716 551 1572 2936 Fragments arranged by size: 2936 1716 1572 551 ApaI G_GGCC'C Cuts at: 4062 4062 Size: 6775 ApaLI G'TGCA_C Cuts at: 4832 5988 4832 Size: 1156 5619 ApoI r'AATT_y Cuts at: 1493 3026 3230 3995 4022 4084 4310 4462 6571 Size: 1533 204 765 27 62 226 152 2109 Cuts at: 6571 1493 Size: 1697 Fragments arranged by size: 2109 1697 1533 765 226 204 152 62 27 AseI AT'TA_AT Cuts at: 160 1032 3015 5329 160 Size: 872 1983 2314 1606 Fragments arranged by size: 2314 1983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 AvaI C'yCGr_G Cuts at: 1870 1943 2485 4100 1870 Size: 73 542 1615 4545 Fragments arranged by size: 4545 1615 542 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2985 3018 2985 Size: 33 6742 BamHI G'GATC_C Cuts at: 4105 4472 4105 Size: 367 6408 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2258 4096 5551 618 Size: 325 217 627 471 1838 1455 1842 Fragments arranged by size: 1842 1838 1455 627 471 325 217 BanII G_rGCy'C Cuts at: 1391 4062 4094 1391 Size: 2671 32 4072 Fragments arranged by size: 4072 2671 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5804 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3616 3650 4966 5000 1903 Size: 34 1679 34 1316 34 3678 Fragments arranged by size: 3678 1679 1316 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4667 5877 4667 Size: 1210 5565 BclI T'GATC_A Cuts at: 1548 1548 Size: 6775 BfrBI ATG'CAT Cuts at: 2948 3268 2948 Size: 320 6455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5386 136 Size: 107 122 71 631 605 3714 1525 Fragments arranged by size: 3714 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3868 6770 3868 Size: 2902 3873 BlpI GC'TnA_GC Cuts at: 4192 4192 Size: 6775 Bme1580I G_kGCm'C Cuts at: 1790 4062 4836 5992 1790 Size: 2272 774 1156 2573 Fragments arranged by size: 2573 2272 1156 774 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6775 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4506 5464 442 Size: 416 53 83 399 24 3089 958 1753 Fragments arranged by size: 3089 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6775 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3531 4065 5435 1239 Size: 2292 534 1370 2579 Fragments arranged by size: 2579 2292 1370 534 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4900 5765 6063 4900 Size: 865 298 5612 Fragments arranged by size: 5612 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5438 882 Size: 617 3939 2219 Fragments arranged by size: 3939 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2620 3052 4566 493 Size: 711 1416 432 1514 2702 Fragments arranged by size: 2702 1514 1416 711 432 BsaBI GATnn'nnATC Cuts at: 6769 6769 Size: 6775 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4582 4964 5599 275 Size: 53 83 186 936 3049 382 635 1451 Fragments arranged by size: 3049 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5208 5606 5880 6027 6438 5208 Size: 398 274 147 411 5545 Fragments arranged by size: 5545 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3091 3121 1178 Size: 30 165 30 1688 30 4832 Fragments arranged by size: 4832 1688 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 2468 1186 Size: 1255 27 5493 Fragments arranged by size: 5493 1255 27 BseYI C'CCAG_C Cuts at: 5978 6474 5978 Size: 496 6279 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6202 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4225 4986 6014 1540 Size: 275 186 2224 761 1028 2301 Fragments arranged by size: 2301 2224 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4836 4921 5992 4836 Size: 85 1071 5619 Fragments arranged by size: 5619 1071 85 BsmI GAATG_Cn' Cuts at: 2950 3220 3648 4286 4379 2950 Size: 270 428 638 93 5346 Fragments arranged by size: 5346 638 428 270 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4062 4094 4836 4921 5992 1391 Size: 399 2272 32 742 85 1071 2174 Fragments arranged by size: 2272 2174 1071 742 399 85 32 BspEI T'CCGG_A Cuts at: 6438 6438 Size: 6775 BspHI T'CATG_A Cuts at: 4664 6394 4664 Size: 1730 5045 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 4130 844 Size: 1415 1871 3489 Fragments arranged by size: 3489 1871 1415 BsrBI CCG'CTC Cuts at: 1394 1823 4049 4662 1394 Size: 429 2226 613 3507 Fragments arranged by size: 3507 2226 613 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2262 3840 5270 5444 65 Size: 1183 1014 1578 1430 174 1396 Fragments arranged by size: 1578 1430 1396 1183 1014 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5419 5606 1700 Size: 110 3609 187 2869 Fragments arranged by size: 3609 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2535 3552 96 Size: 2439 1017 3319 Fragments arranged by size: 3319 2439 1017 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6775 BssSI C'ACGA_G Cuts at: 4835 5847 4835 Size: 1012 5763 BstAPI GCAn_nnn'nTGC Cuts at: 794 2251 3265 3398 794 Size: 1457 1014 133 4171 Fragments arranged by size: 4171 1457 1014 133 BstBI TT'CG_AA Cuts at: 3274 5579 3274 Size: 2305 4470 BstEII G'GTnAC_C Cuts at: 1184 2669 1184 Size: 1485 5290 BstYI r'GATC_y Cuts at: 1972 3832 3868 4105 4472 4857 4874 6326 6412 Size: 1860 36 237 367 385 17 1452 86 Cuts at: 6412 6424 6770 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1860 1452 385 367 346 237 86 36 17 12 BstZ17I GTA'TAC Cuts at: 3055 3055 Size: 6775 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6223 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3636 487 Size: 683 56 2410 3626 Fragments arranged by size: 3626 2410 683 56 BtsI GCAGTG_nn' Cuts at: 3862 4291 5085 5105 3862 Size: 429 794 20 5532 Fragments arranged by size: 5532 794 429 20 ClaI AT'CG_AT Cuts at: 1554 2643 1554 Size: 1089 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6740 DraI TTT'AAA Cuts at: 2743 4079 2743 Size: 1336 5439 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5782 809 Size: 507 4466 1802 Fragments arranged by size: 4466 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3031 3690 4222 Size: 54 1425 50 275 18 1201 659 532 Cuts at: 4222 5111 6740 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1201 889 659 532 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4222 1537 Size: 275 2410 4090 Fragments arranged by size: 4090 2410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2336 2445 3786 3870 4705 1353 Size: 983 109 1341 84 835 3423 Fragments arranged by size: 3423 1341 983 835 109 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5362 5736 5882 387 Size: 1064 138 3773 374 146 1280 Fragments arranged by size: 3773 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 2881 3531 4065 4838 5435 6222 Size: 393 549 700 650 534 773 597 787 Cuts at: 6222 6490 1239 Size: 268 1524 Fragments arranged by size: 1524 787 773 700 650 597 549 534 393 268 EcoO109I rG'GnC_Cy Cuts at: 4040 4040 Size: 6775 EcoRI G'AATT_C Cuts at: 4084 4462 4084 Size: 378 6397 EcoRV GAT'ATC Cuts at: 3538 3538 Size: 6775 FspI TGC'GCA Cuts at: 5281 5281 Size: 6775 HaeII r_GCGC'y Cuts at: 1678 5922 1678 Size: 4244 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2606 2638 2741 Size: 32 135 32 563 32 439 32 103 Cuts at: 2741 2773 2869 2901 3935 3967 5416 5448 5490 Size: 32 96 32 1034 32 1449 32 42 Cuts at: 5490 5522 1373 Size: 32 2626 Fragments arranged by size: 2626 1449 1034 563 439 135 103 96 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4119 4365 669 Size: 988 281 2181 246 3079 Fragments arranged by size: 3079 2181 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6775 HpaI GTT'AAC Cuts at: 4365 4365 Size: 6775 KpnI G_GTAC'C Cuts at: 4100 4100 Size: 6775 MfeI C'AATT_G Cuts at: 4374 4374 Size: 6775 MluI A'CGCG_T Cuts at: 6651 6651 Size: 6775 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2754 2931 3231 5889 6073 1305 Size: 1449 177 300 2658 184 2007 Fragments arranged by size: 2658 2007 1449 300 184 177 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4953 Fragments arranged by size: 4953 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2943 4733 5092 5251 518 Size: 648 1777 1790 359 159 2042 Fragments arranged by size: 2042 1790 1777 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4191 4868 6016 6261 1606 Size: 2585 677 1148 245 2120 Fragments arranged by size: 2585 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6223 NdeI CA'TA_TG Cuts at: 387 387 Size: 6775 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6775 NotI GC'GGCC_GC Cuts at: 4222 4222 Size: 6775 NsiI A_TGCA'T Cuts at: 2950 3270 2950 Size: 320 6455 NspI r_CATG'y Cuts at: 1132 1229 2121 2568 2952 4133 5678 1132 Size: 97 892 447 384 1181 1545 2229 Fragments arranged by size: 2229 1545 1181 892 447 384 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6775 PciI A'CATG_T Cuts at: 5674 5674 Size: 6775 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6775 PfoI T'CCnGG_A Cuts at: 3508 3508 Size: 6775 PmeI GTTT'AAAC Cuts at: 4079 4079 Size: 6775 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2694 2726 4844 4876 6357 6389 2694 Size: 32 2118 32 1481 32 3080 Fragments arranged by size: 3080 2118 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4040 4040 Size: 6775 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6775 PsiI TTA'TAA Cuts at: 2208 4345 2208 Size: 2137 4638 PspOMI G'GGCC_C Cuts at: 4058 4058 Size: 6775 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3178 3210 3178 Size: 32 6743 PstI C_TGCA'G Cuts at: 830 4127 830 Size: 3297 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6775 SalI G'TCGA_C Cuts at: 1936 4117 1936 Size: 2181 4594 SapI GCTCTTCn'nnn_ Cuts at: 2336 2336 Size: 6775 SbfI CC_TGCA'GG Cuts at: 4127 4127 Size: 6775 ScaI AGT'ACT Cuts at: 5023 6735 5023 Size: 1712 5063 SexAI A'CCwGG_T Cuts at: 3920 3920 Size: 6775 SfcI C'TryA_G Cuts at: 826 1045 3502 4071 4123 5258 5939 6130 826 Size: 219 2457 569 52 1135 681 191 1471 Fragments arranged by size: 2457 1471 1135 681 569 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6775 SmaI CCC'GGG Cuts at: 1872 4102 1872 Size: 2230 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4879 5780 6042 820 Size: 197 926 2936 901 262 1553 Fragments arranged by size: 2936 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2620 3052 4566 493 Size: 2127 432 1514 2702 Fragments arranged by size: 2702 2127 1514 432 SpeI A'CTAG_T Cuts at: 152 152 Size: 6775 SphI G_CATG'C Cuts at: 1132 2121 4133 1132 Size: 989 2012 3774 Fragments arranged by size: 3774 2012 989 SspI AAT'ATT Cuts at: 5 52 2029 2600 3697 4699 5 Size: 47 1977 571 1097 1002 2081 Fragments arranged by size: 2081 1977 1097 1002 571 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6775 StyI C'CwwG_G Cuts at: 513 1065 3984 4214 513 Size: 552 2919 230 3074 Fragments arranged by size: 3074 2919 552 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4802 4819 4972 5157 5602 325 Size: 1255 3222 17 153 185 445 1498 Fragments arranged by size: 3222 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2535 3552 5021 Size: 274 80 33 51 1443 558 1017 1469 Cuts at: 5021 6733 96 Size: 1712 138 Fragments arranged by size: 1712 1469 1443 1017 558 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2935 2967 6735 6767 2935 Size: 32 3768 32 2943 Fragments arranged by size: 3768 2943 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2630 1316 Size: 1314 5461 XbaI T'CTAG_A Cuts at: 4111 4111 Size: 6775 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3784 3784 Size: 6775 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6775 XmaI C'CCGG_G Cuts at: 1870 4100 1870 Size: 2230 4545 XmnI GAAnn'nnTTC Cuts at: 4904 4904 Size: 6775 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4583 276 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI