(Linear) (Six-base) MAPSORT of: KIEE5721.seq Check: 2860 from: 1 to: 2070 KIEE5721.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AccI GT'mk_AC Cuts at: 0 998 1049 1066 1496 1821 1901 2070 Size: 998 51 17 430 325 80 169 Fragments arranged by size: 998 430 325 169 80 51 17 AclI AA'CG_TT Cuts at: 0 1403 2070 Size: 1403 667 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 176 876 2070 Size: 176 700 1194 Fragments arranged by size: 1194 700 176 AlwNI CAG_nnn'CTG Cuts at: 0 246 1962 2070 Size: 246 1716 108 Fragments arranged by size: 1716 246 108 ApaI G_GGCC'C Cuts at: 0 2057 2070 Size: 2057 13 ApoI r'AATT_y Cuts at: 0 1021 1225 1990 2017 2070 Size: 1021 204 765 27 53 Fragments arranged by size: 1021 765 204 53 27 AseI AT'TA_AT Cuts at: 0 1010 2070 Size: 1010 1060 AvaI C'yCGr_G Cuts at: 0 480 2070 Size: 480 1590 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 980 1013 2070 Size: 980 33 1057 Fragments arranged by size: 1057 980 33 BanI G'GyrC_C Cuts at: 0 253 2070 Size: 253 1817 BanII G_rGCy'C Cuts at: 0 2057 2070 Size: 2057 13 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1611 1645 2070 Size: 1611 34 425 Fragments arranged by size: 1611 425 34 BfrBI ATG'CAT Cuts at: 0 943 1263 2070 Size: 943 320 807 Fragments arranged by size: 943 807 320 BglII A'GATC_T Cuts at: 0 1863 2070 Size: 1863 207 Bme1580I G_kGCm'C Cuts at: 0 2057 2070 Size: 2057 13 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1526 2060 2070 Size: 1526 534 10 Fragments arranged by size: 1526 534 10 BsaAI yAC'GTr Cuts at: 0 615 1047 2070 Size: 615 432 1023 Fragments arranged by size: 1023 615 432 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1086 1116 2070 Size: 1086 30 954 Fragments arranged by size: 1086 954 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 436 463 2070 Size: 436 27 1607 Fragments arranged by size: 1607 436 27 BsmI GAATG_Cn' Cuts at: 0 945 1215 1643 2070 Size: 945 270 428 427 Fragments arranged by size: 945 428 427 270 Bsp1286I G_dGCh'C Cuts at: 0 2057 2070 Size: 2057 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 254 2070 Size: 254 1816 BsrBI CCG'CTC Cuts at: 0 2044 2070 Size: 2044 26 BsrDI GCAATG_nn' Cuts at: 0 257 1835 2070 Size: 257 1578 235 Fragments arranged by size: 1578 257 235 BsrGI T'GTAC_A Cuts at: 0 530 1547 2070 Size: 530 1017 523 Fragments arranged by size: 1017 530 523 BstAPI GCAn_nnn'nTGC Cuts at: 0 246 1260 1393 2070 Size: 246 1014 133 677 Fragments arranged by size: 1014 677 246 133 BstBI TT'CG_AA Cuts at: 0 1269 2070 Size: 1269 801 BstEII G'GTnAC_C Cuts at: 0 664 2070 Size: 664 1406 BstYI r'GATC_y Cuts at: 0 1827 1863 2070 Size: 1827 36 207 Fragments arranged by size: 1827 207 36 BstZ17I GTA'TAC Cuts at: 0 1050 2070 Size: 1050 1020 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1631 2070 Size: 1631 439 BtsI GCAGTG_nn' Cuts at: 0 1857 2070 Size: 1857 213 ClaI AT'CG_AT Cuts at: 0 638 2070 Size: 638 1432 DraI TTT'AAA Cuts at: 0 738 2070 Size: 738 1332 EaeI y'GGCC_r Cuts at: 0 1026 1685 2070 Size: 1026 659 385 Fragments arranged by size: 1026 659 385 EarI CTCTTCn'nnn_ Cuts at: 0 331 440 1781 1865 2070 Size: 331 109 1341 84 205 Fragments arranged by size: 1341 331 205 109 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 176 876 1526 2060 2070 Size: 176 700 650 534 10 Fragments arranged by size: 700 650 534 176 10 EcoO109I rG'GnC_Cy Cuts at: 0 2035 2070 Size: 2035 35 EcoRV GAT'ATC Cuts at: 0 1533 2070 Size: 1533 537 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 130 162 601 633 736 768 864 896 Size: 130 32 439 32 103 32 96 32 Cuts at: 896 1930 1962 2070 Size: 1034 32 108 Fragments arranged by size: 1034 439 130 108 103 96 32 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 749 926 1226 2070 Size: 749 177 300 844 Fragments arranged by size: 844 749 300 177 MslI CAynn'nnrTG Cuts at: 0 938 2070 Size: 938 1132 NsiI A_TGCA'T Cuts at: 0 945 1265 2070 Size: 945 320 805 Fragments arranged by size: 945 805 320 NspI r_CATG'y Cuts at: 0 116 563 947 2070 Size: 116 447 384 1123 Fragments arranged by size: 1123 447 384 116 PfoI T'CCnGG_A Cuts at: 0 1503 2070 Size: 1503 567 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 689 721 2070 Size: 689 32 1349 Fragments arranged by size: 1349 689 32 PpuMI rG'GwC_Cy Cuts at: 0 2035 2070 Size: 2035 35 PsiI TTA'TAA Cuts at: 0 203 2070 Size: 203 1867 PspOMI G'GGCC_C Cuts at: 0 2053 2070 Size: 2053 17 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1173 1205 2070 Size: 1173 32 865 Fragments arranged by size: 1173 865 32 SapI GCTCTTCn'nnn_ Cuts at: 0 331 2070 Size: 331 1739 SexAI A'CCwGG_T Cuts at: 0 1915 2070 Size: 1915 155 SfcI C'TryA_G Cuts at: 0 1497 2070 Size: 1497 573 SnaBI TAC'GTA Cuts at: 0 615 1047 2070 Size: 615 432 1023 Fragments arranged by size: 1023 615 432 SphI G_CATG'C Cuts at: 0 116 2070 Size: 116 1954 SspI AAT'ATT Cuts at: 0 24 595 1692 2070 Size: 24 571 1097 378 Fragments arranged by size: 1097 571 378 24 StyI C'CwwG_G Cuts at: 0 1979 2070 Size: 1979 91 TatI w'GTAC_w Cuts at: 0 530 1547 2070 Size: 530 1017 523 Fragments arranged by size: 1017 530 523 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 930 962 2070 Size: 930 32 1108 Fragments arranged by size: 1108 930 32 Tth111I GACn'n_nGTC Cuts at: 0 625 2070 Size: 625 1445 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1779 2070 Size: 1779 291 Enzymes that do cut: AccI AclI AcuI AlwNI ApaI ApoI AseI AvaI BaeI BanI BanII BcgI BfrBI BglII Bme1580I BpmI BsaAI BsaXI BseRI BsmI Bsp1286I BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstYI BstZ17I BtgZI BtsI ClaI DraI EaeI EarI Eco57MI EcoO109I EcoRV Hin4I MmeI MslI NsiI NspI PfoI PpiI PpuMI PsiI PspOMI PsrI SapI SexAI SfcI SnaBI SphI SspI StyI TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AsiSI AvrII BamHI BbeI BbsI BbvCI BciVI BclI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrFI BssHII BssSI BstXI Bsu36I BtgI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PshAI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SrfI StuI SwaI TaqII XbaI XhoI XmaI XmnI ZraI