Restriction map for
pFN21AE1956
##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 550 GTAGAC GT^[AC][GT]AC
AcsI 968 GAATTT [GA]^AATT[CT]
AflIII 379 ACATGT A^C[GA][CT]GT
AhdI 640 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1317 GGGCCC GGGCC^C
ApoI 968 GAATTT [GA]^AATT[CT]
AspEI 640 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1183 GAGCAC G[AT]GC[AT]^C
AvaII 127 GGACC G^G[AT]CC
BanI 53 GGTGCC G^G[CT][GA]CC
BbsI 501 GAAGAC GAAGAC(2/6)
BclI 617 TGATCA T^GATCA
BcnI 57 CCGGG CC^[GC]GG
BglI 28 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 9 CTCCAG CTGGAG(16/14)
BpuAI 501 GAAGAC GAAGAC(2/6)
BsaI 1453 GAGACC GGTCTC(1/5)
BsiHKAI 1183 GAGCAC G[AT]GC[AT]^C
BspLU11I 379 ACATGT A^CATGT
BstEII 150 GGTGACC G^GT[ACGT]ACC
BstPI 150 GGTGACC G^GT[ACGT]ACC
BstXI 400 CCAGATGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 47 GCGC GCG^C
DsaI 29 CCGTGG C^C[GA][CT]GG
Eam1105I 640 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
FbaI 617 TGATCA T^GATCA
HapII 57 CCGG C^CGG
HhaI 47 GCGC GCG^C
HinP1I 47 GCGC G^CGC
HindIII 987 AAGCTT A^AGCTT
HpaII 57 CCGG C^CGG
MspA1I 112 CCGCTG C[AC]G^C[GT]G
MspI 57 CCGG C^CGG
NciI 57 CCGGG CC^[GC]GG
NspI 379 ACATGT [GA]CATG^[CT]
PleI 357 GACTC GAGTC(4/5)
SexAI 1007 ACCAGGT A^CC[AT]GGT
TaqI 1056 TCGA T^CGA
TthHB8I 1056 TCGA T^CGA
XbaI 1164 TCTAGA T^CTAGA
XcmI 1521 CCATCAATATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 66 CTCGGG C^[CT]CG[GA]G
269 CCCGAG
BanII 1317 GGGCCC G[GA]GC[CT]^C
1517 GAGCCC
BfaI 257 CTAG C^TAG
1165 CTAG
BsmAI 1453 GAGAC GTCTC(1/5)
1065 GTCTC
BsmFI 103 GTCCC GGGAC(10/14)
306 GTCCC
BsoBI 66 CTCGGG C^[CT]CG[GA]G
269 CCCGAG
BsrDI 1228 CATTGC GCAATG(2/0)
1380 CATTGC
BstF5I 1202 CATCC GGATG(2/0)
1209 GGATG
DdeI 359 CTCAG C^T[ACGT]AG
1391 CTAAG
DraII 1316 AGGGCCC [GA]G^G[ACGT]CC[CT]
1317 GGGCCCC
EarI 134 CTCTTC CTCTTC(1/4)
578 GAAGAG
EcoO109I 1316 AGGGCCC [GA]G^G[ACGT]CC[CT]
1317 GGGCCCC
FauI 106 CCCGC CCCGC(4/6)
111 CCCGC
FokI 1202 CATCC GGATG(9/13)
1209 GGATG
Ksp632I 134 CTCTTC CTCTTC(1/4)
578 GAAGAG
MaeI 257 CTAG C^TAG
1165 CTAG
MslI 813 CATTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1306 CATTCTTGTG
PflMI 932 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
993 CCAGATGCTGG
ScaI 582 AGTACT AGT^ACT
1366 AGTACT
SfcI 198 CTGTAG C^T[GA][CT]AG
1272 CTGTAG
Van91I 932 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
993 CCAGATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BamHI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
BstYI Cfr13I DpnII EcoRII EcoT14I
Fnu4HI HaeIII HincII HindII HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII