(Circular) (Six-base) MAPSORT of: pFN21AE1956.seq Check: 7670 from: 1 to: 6244 pFN21AE1956 6244 bp With 182 enzymes: SgfI * March 28, 2012 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4054 278 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2556 3587 1937 Size: 619 1031 4594 Fragments arranged by size: 4594 1031 619 Acc65I G'GTAC_C Cuts at: 3565 3565 Size: 6244 AclI AA'CG_TT Cuts at: 1563 4371 4744 1563 Size: 2808 373 3063 Fragments arranged by size: 3063 2808 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3144 4307 5691 5959 1632 Size: 1512 1163 1384 268 1917 Fragments arranged by size: 1917 1512 1384 1163 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6047 AflIII A'CryG_T Cuts at: 1203 2384 5143 6120 1203 Size: 1181 2759 977 1327 Fragments arranged by size: 2759 1327 1181 977 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 2650 4973 2650 Size: 2323 3921 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6244 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6244 AlwNI CAG_nnn'CTG Cuts at: 2440 2524 3004 3987 5559 2440 Size: 84 480 983 1572 3125 Fragments arranged by size: 3125 1572 983 480 84 ApaI G_GGCC'C Cuts at: 3326 3326 Size: 6244 ApaLI G'TGCA_C Cuts at: 4301 5457 4301 Size: 1156 5088 ApoI r'AATT_y Cuts at: 1493 2973 3553 3779 3931 6040 1493 Size: 1480 580 226 152 2109 1697 Fragments arranged by size: 2109 1697 1480 580 226 152 AseI AT'TA_AT Cuts at: 160 1032 4798 160 Size: 872 3766 1606 Fragments arranged by size: 3766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 2071 2274 3569 1870 Size: 73 128 203 1295 4545 Fragments arranged by size: 4545 1295 203 128 73 BamHI G'GATC_C Cuts at: 2098 2830 3270 3574 3941 2098 Size: 732 440 304 367 4401 Fragments arranged by size: 4401 732 440 367 304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2058 3565 5020 618 Size: 325 217 627 271 1507 1455 1842 Fragments arranged by size: 1842 1507 1455 627 325 271 217 BanII G_rGCy'C Cuts at: 1391 3326 3526 3563 1391 Size: 1935 200 37 4072 Fragments arranged by size: 4072 1935 200 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2513 928 Size: 971 614 4659 Fragments arranged by size: 4659 971 614 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4435 4469 1903 Size: 34 2498 34 3678 Fragments arranged by size: 3678 2498 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4136 5346 4136 Size: 1210 5034 BclI T'GATC_A Cuts at: 1548 2622 1548 Size: 1074 5170 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2039 4855 Size: 107 122 71 631 605 337 30 2816 Cuts at: 4855 136 Size: 1525 Fragments arranged by size: 2816 1525 631 605 337 122 107 71 30 BglII A'GATC_T Cuts at: 6239 6239 Size: 6244 BlpI GC'TnA_GC Cuts at: 3661 3661 Size: 6244 Bme1580I G_kGCm'C Cuts at: 1790 2126 3326 4305 5461 1790 Size: 336 1200 979 1156 2573 Fragments arranged by size: 2573 1200 1156 979 336 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6244 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2422 2455 2469 Size: 416 53 83 399 24 1005 33 14 Cuts at: 2469 2512 3975 4933 442 Size: 43 1463 958 1753 Fragments arranged by size: 1753 1463 1005 958 416 399 83 53 43 33 24 14 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 4904 1239 Size: 760 2905 2579 Fragments arranged by size: 2905 2579 760 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4369 5234 5532 4369 Size: 865 298 5081 Fragments arranged by size: 5081 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3452 4907 882 Size: 617 1953 1455 2219 Fragments arranged by size: 2219 1953 1455 617 BsaAI yAC'GTr Cuts at: 493 1204 4035 493 Size: 711 2831 2702 Fragments arranged by size: 2831 2702 711 BsaBI GATnn'nnATC Cuts at: 6238 6238 Size: 6244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4051 4433 5068 275 Size: 53 83 186 936 2518 382 635 1451 Fragments arranged by size: 2518 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4677 5075 5349 5496 5907 4677 Size: 398 274 147 411 5014 Fragments arranged by size: 5014 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2818 2848 1178 Size: 30 165 30 1415 30 4574 Fragments arranged by size: 4574 1415 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6244 BseYI C'CCAG_C Cuts at: 5447 5943 5447 Size: 496 5748 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5671 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3694 4455 5483 1540 Size: 275 186 1693 761 1028 2301 Fragments arranged by size: 2301 1693 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3192 4305 4390 5461 3192 Size: 1113 85 1071 3975 Fragments arranged by size: 3975 1113 1071 85 BsmI GAATG_Cn' Cuts at: 3755 3848 3755 Size: 93 6151 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 3192 3326 3526 3563 4305 4390 Size: 399 336 1066 134 200 37 742 85 Cuts at: 4390 5461 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1066 742 399 336 200 134 85 37 BspEI T'CCGG_A Cuts at: 5907 5907 Size: 6244 BspHI T'CATG_A Cuts at: 4133 5863 4133 Size: 1730 4514 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3599 844 Size: 2755 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4131 1394 Size: 429 2308 3507 Fragments arranged by size: 3507 2308 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3232 3384 4739 4913 65 Size: 1183 1984 152 1355 174 1396 Fragments arranged by size: 1984 1396 1355 1183 174 152 BsrFI r'CCGG_y Cuts at: 1700 1810 4888 5075 1700 Size: 110 3078 187 2869 Fragments arranged by size: 3078 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6244 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6244 BssSI C'ACGA_G Cuts at: 4304 5316 4304 Size: 1012 5232 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6244 BstBI TT'CG_AA Cuts at: 5048 5048 Size: 6244 BstEII G'GTnAC_C Cuts at: 1184 2155 1184 Size: 971 5273 BstXI CCAn_nnnn'nTGG Cuts at: 2412 2412 Size: 6244 BstYI r'GATC_y Cuts at: 1972 2098 2830 3270 3574 3941 4326 4343 5795 Size: 126 732 440 304 367 385 17 1452 Cuts at: 5795 5881 5893 6239 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 732 440 385 367 346 304 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 513 Size: 552 939 30 4723 Fragments arranged by size: 4723 939 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5505 Fragments arranged by size: 5505 683 56 BtsI GCAGTG_nn' Cuts at: 2327 3760 4554 4574 2327 Size: 1433 794 20 3997 Fragments arranged by size: 3997 1433 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6244 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 3548 3548 Size: 6244 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5251 809 Size: 507 3935 1802 Fragments arranged by size: 3935 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3691 4580 6209 Size: 54 1425 50 275 18 1861 889 1629 Cuts at: 6209 8 Size: 43 Fragments arranged by size: 1861 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3691 1537 Size: 275 1879 4090 Fragments arranged by size: 4090 1879 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 2578 4174 1353 Size: 792 433 1596 3423 Fragments arranged by size: 3423 1596 792 433 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3087 4831 5205 5351 387 Size: 1064 138 1498 1744 374 146 1280 Fragments arranged by size: 1744 1498 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 3144 4307 4904 5691 5959 1239 Size: 393 367 1145 1163 597 787 268 1524 Fragments arranged by size: 1524 1163 1145 787 597 393 367 268 EcoO109I rG'GnC_Cy Cuts at: 3322 3323 3322 Size: 1 6243 EcoRI G'AATT_C Cuts at: 3553 3931 3553 Size: 378 5866 FspI TGC'GCA Cuts at: 4750 4750 Size: 6244 HaeII r_GCGC'y Cuts at: 1678 5391 1678 Size: 3713 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2636 2668 2818 2850 4885 Size: 32 135 32 1064 32 150 32 2035 Cuts at: 4885 4917 4959 4991 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2035 1064 150 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2480 2631 3155 3588 3834 669 Size: 988 281 542 151 524 433 246 3079 Fragments arranged by size: 3079 988 542 524 433 281 246 151 HindIII A'AGCT_T Cuts at: 748 2992 748 Size: 2244 4000 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KpnI G_GTAC'C Cuts at: 3569 3569 Size: 6244 MfeI C'AATT_G Cuts at: 3843 3843 Size: 6244 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2578 5358 5542 1305 Size: 1273 2780 184 2007 Fragments arranged by size: 2780 2007 1273 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4422 Fragments arranged by size: 4422 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2822 3315 4202 4561 4720 518 Size: 648 1656 493 887 359 159 2042 Fragments arranged by size: 2042 1656 887 648 493 359 159 MspA1I CmG'CkG Cuts at: 1606 2119 3660 4337 5485 5730 1606 Size: 513 1541 677 1148 245 2120 Fragments arranged by size: 2120 1541 1148 677 513 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4753 Fragments arranged by size: 4753 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 3691 3691 Size: 6244 NspI r_CATG'y Cuts at: 1132 1229 2388 3602 5147 1132 Size: 97 1159 1214 1545 2229 Fragments arranged by size: 2229 1545 1214 1159 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6244 PciI A'CATG_T Cuts at: 2384 5143 2384 Size: 2759 3485 PflMI CCAn_nnn'nTGG Cuts at: 1108 2943 3004 1108 Size: 1835 61 4348 Fragments arranged by size: 4348 1835 61 PfoI T'CCnGG_A Cuts at: 2015 3266 2015 Size: 1251 4993 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4313 4345 5826 5858 4313 Size: 32 1481 32 4699 Fragments arranged by size: 4699 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6244 PsiI TTA'TAA Cuts at: 2730 3042 3814 2730 Size: 312 772 5160 Fragments arranged by size: 5160 772 312 PspOMI G'GGCC_C Cuts at: 3322 3322 Size: 6244 PstI C_TGCA'G Cuts at: 830 3596 830 Size: 2766 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6244 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SbfI CC_TGCA'GG Cuts at: 3596 3596 Size: 6244 ScaI AGT'ACT Cuts at: 2589 3373 4492 6204 2589 Size: 784 1119 1712 2629 Fragments arranged by size: 2629 1712 1119 784 SexAI A'CCwGG_T Cuts at: 3012 3012 Size: 6244 SfcI C'TryA_G Cuts at: 826 1045 2203 3277 3592 4727 5408 5599 826 Size: 219 1158 1074 315 1135 681 191 1471 Fragments arranged by size: 1471 1158 1135 1074 681 315 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 3571 1872 Size: 1699 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4348 5249 5511 820 Size: 197 926 2405 901 262 1553 Fragments arranged by size: 2405 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4035 493 Size: 3542 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6244 SphI G_CATG'C Cuts at: 1132 3602 1132 Size: 2470 3774 SspI AAT'ATT Cuts at: 5 52 4168 5 Size: 47 4116 2081 Fragments arranged by size: 4116 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6244 StyI C'CwwG_G Cuts at: 513 1065 2004 2161 2606 3210 3683 513 Size: 552 939 157 445 604 473 3074 Fragments arranged by size: 3074 939 604 552 473 445 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4271 4288 4441 4626 5071 325 Size: 1255 2691 17 153 185 445 1498 Fragments arranged by size: 2691 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2587 3371 4490 Size: 274 80 33 51 1443 610 784 1119 Cuts at: 4490 6202 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1119 784 610 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2067 2099 3079 3111 6204 6236 2067 Size: 32 980 32 3093 32 2075 Fragments arranged by size: 3093 2075 980 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6244 XbaI T'CTAG_A Cuts at: 3169 3580 3169 Size: 411 5833 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3533 3533 Size: 6244 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6244 XmaI C'CCGG_G Cuts at: 1870 3569 1870 Size: 1699 4545 XmnI GAAnn'nnTTC Cuts at: 4373 4373 Size: 6244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4052 276 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI