################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AlwNI AocI ApaI AscI Asp718I BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BlpI Bpu1102I BsaI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I DrdI EagI EclXI Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII SacII SalI SapI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SpeI SphI SspBI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AflIII 118 ACATGT A^C[GA][CT]GT AhdI 1894 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 312 GTGCAC G^TGCAC ApaLI 312 GTGCAC G^TGCAC AseI 2053 ATTAAT AT^TAAT AsnI 2053 ATTAAT AT^TAAT Asp700I 924 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1894 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1668 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 774 CCCGAG C^[CT]CG[GA]G AviII 527 TGCGCA TGC^GCA AvrII 1995 CCTAGG C^CTAGG BanII 807 GAGCTC G[GA]GC[CT]^C BbeI 517 GGCGCC GGCGC^C BbsI 1538 GAAGAC GAAGAC(2/6) BglI 844 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1995 CCTAGG C^CTAGG BpuAI 1538 GAAGAC GAAGAC(2/6) BsaAI 568 TACGTA [CT]AC^GT[GA] BsaBI 1943 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1627 TCCGGT [AT]^CCGG[AT] BsmI 1657 GCATTC GAATGC(1/-1) BsoBI 774 CCCGAG C^[CT]CG[GA]G BspDI 1775 ATCGAT AT^CGAT BspLU11I 118 ACATGT A^CATGT BssSI 613 CTCGTG CACGAG(-5/-1) BstEII 252 GGTGACC G^GT[ACGT]ACC BstPI 252 GGTGACC G^GT[ACGT]ACC ClaI 1775 ATCGAT AT^CGAT CpoI 825 CGGACCG CG^G[AT]CCG DraI 75 TTTAAA TTT^AAA DraIII 985 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 699 CCGTGG C^C[GA][CT]GG Eam1105I 1894 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 252 GGTGACC G^GT[ACGT]ACC EcoRI 1192 GAATTC G^AATTC FspI 527 TGCGCA TGC^GCA HindIII 2156 AAGCTT A^AGCTT KasI 517 GGCGCC G^GCGCC MamI 1943 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 517 GGCGCC GG^CGCC NdeI 1145 CATATG CA^TATG NspI 118 ACATGT [GA]CATG^[CT] PflFI 1668 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1091 CCAAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 2053 ATTAAT AT^TAAT RsrII 825 CGGACCG CG^G[AT]CCG SacI 807 GAGCTC GAGCT^C SbfI 860 CCTGCAGG CCTGCA^GG SfoI 517 GGCGCC GGC^GCC SnaBI 568 TACGTA TAC^GTA Sse8387I 860 CCTGCAGG CCTGCA^GG StuI 1886 AGGCCT AGG^CCT TaqI 1776 TCGA T^CGA Tth111I 1668 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1776 TCGA T^CGA Van91I 1091 CCAAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 924 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 517 GGCGCC G[GA]^CG[CT]C 1155 GGCGTC AfaI 571 GTAC GT^AC 591 GTAC AlwI 939 GATCC GGATC(4/5) 1778 GATCC Aor51HI 926 AGCGCT AGC^GCT 945 AGCGCT BanI 517 GGCGCC G^G[CT][GA]CC 892 GGCACC BglII 1569 AGATCT A^GATCT 1942 AGATCT BsaHI 517 GGCGCC G[GA]^CG[CT]C 1155 GGCGTC BseRI 610 CTCCTC GAGGAG(10/8) 658 GAGGAG BstXI 203 CCATGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1823 CCACGTTTGTGG BstYI 1569 AGATCT [GA]^GATC[CT] 1942 AGATCT EarI 1552 GAAGAG CTCTTC(1/4) 1882 GAAGAG Eco47III 926 AGCGCT AGC^GCT 945 AGCGCT HgaI 242 GCGTC GACGC(5/10) 1156 GCGTC Hin1I 517 GGCGCC G[GA]^CG[CT]C 1155 GGCGTC Ksp632I 1552 GAAGAG CTCTTC(1/4) 1882 GAAGAG MflI 1569 AGATCT [GA]^GATC[CT] 1942 AGATCT PpuMI 287 AGGTCCC [GA]G^G[AT]CC[CT] 718 GGGACCC PstI 861 CTGCAG CTGCA^G 1131 CTGCAG RsaI 571 GTAC GT^AC 591 GTAC Tsp45I 253 GTGAC ^GT[GC]AC 1991 GTGAC XhoII 1569 AGATCT [GA]^GATC[CT] 1942 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AluI ApoI AspHI AvaII BalI BbvI BcnI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RcaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI XcmI