(Circular) (Six-base) MAPSORT of: pFN21AE9458.seq Check: 9050 from: 1 to: 6898 pFN21AE9458 6898 bp With 182 enzymes: SgfI * March 28, 2012 12:10 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4708 278 Size: 53 83 186 936 3172 2468 Fragments arranged by size: 3172 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4241 1937 Size: 2304 4594 Acc65I G'GTAC_C Cuts at: 4219 4219 Size: 6898 AclI AA'CG_TT Cuts at: 1563 5025 5398 1563 Size: 3462 373 3063 Fragments arranged by size: 3462 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2880 3549 4961 6345 6613 1632 Size: 1248 669 1412 1384 268 1917 Fragments arranged by size: 1917 1412 1384 1248 669 268 AfeI AGC'GCT Cuts at: 2933 2952 2933 Size: 19 6879 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6701 AflIII A'CryG_T Cuts at: 1203 2123 5797 6774 1203 Size: 920 3674 977 1327 Fragments arranged by size: 3674 1327 977 920 AgeI A'CCGG_T Cuts at: 5729 5729 Size: 6898 AhdI GACnn_n'nnGTC Cuts at: 3904 5627 3904 Size: 1723 5175 AleI CACnn'nnGTG Cuts at: 1166 2412 3833 3845 1166 Size: 1246 1421 12 4219 Fragments arranged by size: 4219 1421 1246 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6898 AlwNI CAG_nnn'CTG Cuts at: 4641 6213 4641 Size: 1572 5326 ApaLI G'TGCA_C Cuts at: 2317 4955 6111 2317 Size: 2638 1156 3104 Fragments arranged by size: 3104 2638 1156 ApoI r'AATT_y Cuts at: 1493 2697 3197 3271 3873 4207 4433 4585 6694 Size: 1204 500 74 602 334 226 152 2109 Cuts at: 6694 1493 Size: 1697 Fragments arranged by size: 2109 1697 1204 602 500 334 226 152 74 AseI AT'TA_AT Cuts at: 160 1032 4059 5452 160 Size: 872 3027 1393 1606 Fragments arranged by size: 3027 1606 1393 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6898 AvaI C'yCGr_G Cuts at: 1870 1943 2779 4223 1870 Size: 73 836 1444 4545 Fragments arranged by size: 4545 1444 836 73 AvrII C'CTAG_G Cuts at: 4000 4000 Size: 6898 BamHI G'GATC_C Cuts at: 4228 4595 4228 Size: 367 6531 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2522 2897 4219 5674 618 Size: 325 217 627 735 375 1322 1455 1842 Fragments arranged by size: 1842 1455 1322 735 627 375 325 217 BanII G_rGCy'C Cuts at: 1391 2816 4217 1391 Size: 1425 1401 4072 Fragments arranged by size: 4072 1425 1401 BbeI G_GCGC'C Cuts at: 2526 2526 Size: 6898 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3550 928 Size: 971 1651 4276 Fragments arranged by size: 4276 1651 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5089 5123 1903 Size: 34 3152 34 3678 Fragments arranged by size: 3678 3152 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4790 6000 4790 Size: 1210 5688 BclI T'GATC_A Cuts at: 1548 1548 Size: 6898 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2855 5509 136 Size: 107 122 71 631 605 1183 2654 1525 Fragments arranged by size: 2654 1525 1183 631 605 122 107 71 BglII A'GATC_T Cuts at: 3574 3947 6893 3574 Size: 373 2946 3579 Fragments arranged by size: 3579 2946 373 BlpI GC'TnA_GC Cuts at: 4315 4315 Size: 6898 Bme1580I G_kGCm'C Cuts at: 1790 2321 4959 6115 1790 Size: 531 2638 1156 2573 Fragments arranged by size: 2638 2573 1156 531 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6898 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3074 3595 4629 Size: 416 53 83 399 24 1657 521 1034 Cuts at: 4629 5587 442 Size: 958 1753 Fragments arranged by size: 1753 1657 1034 958 521 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6898 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2419 2548 2757 3738 3952 5558 1239 Size: 1180 129 209 981 214 1606 2579 Fragments arranged by size: 2579 1606 1180 981 214 209 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5023 5888 6186 5023 Size: 865 298 5735 Fragments arranged by size: 5735 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5561 882 Size: 617 4062 2219 Fragments arranged by size: 4062 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2575 4689 493 Size: 711 1371 2114 2702 Fragments arranged by size: 2702 2114 1371 711 BsaBI GATnn'nnATC Cuts at: 3952 6892 3952 Size: 2940 3958 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2523 3161 4705 5087 Size: 53 83 186 936 990 638 1544 382 Cuts at: 5087 5722 275 Size: 635 1451 Fragments arranged by size: 1544 1451 990 936 638 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3632 5331 5729 6003 6150 6561 3632 Size: 1699 398 274 147 411 3969 Fragments arranged by size: 3969 1699 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6673 Fragments arranged by size: 6673 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2606 2678 1186 Size: 1420 72 5406 Fragments arranged by size: 5406 1420 72 BseYI C'CCAG_C Cuts at: 2517 2852 6101 6597 2517 Size: 335 3249 496 2818 Fragments arranged by size: 3249 2818 496 335 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6325 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4348 5109 6137 1540 Size: 275 186 2347 761 1028 2301 Fragments arranged by size: 2347 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2321 2457 2816 4959 5044 6115 2321 Size: 136 359 2143 85 1071 3104 Fragments arranged by size: 3104 2143 1071 359 136 85 BsmI GAATG_Cn' Cuts at: 3662 4409 4502 3662 Size: 747 93 6058 Fragments arranged by size: 6058 747 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2321 2457 2816 4217 4959 5044 6115 Size: 399 531 136 359 1401 742 85 1071 Cuts at: 6115 1391 Size: 2174 Fragments arranged by size: 2174 1401 1071 742 531 399 359 136 85 BspEI T'CCGG_A Cuts at: 6561 6561 Size: 6898 BspHI T'CATG_A Cuts at: 2130 2241 3553 4787 6517 2130 Size: 111 1312 1234 1730 2511 Fragments arranged by size: 2511 1730 1312 1234 111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4253 844 Size: 3409 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4785 1394 Size: 429 2962 3507 Fragments arranged by size: 3507 2962 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5393 5567 65 Size: 1183 4145 174 1396 Fragments arranged by size: 4145 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5542 5729 1700 Size: 110 3732 187 2869 Fragments arranged by size: 3732 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6898 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6898 BssSI C'ACGA_G Cuts at: 2618 4958 5970 2618 Size: 2340 1012 3546 Fragments arranged by size: 3546 2340 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6898 BstBI TT'CG_AA Cuts at: 5702 5702 Size: 6898 BstEII G'GTnAC_C Cuts at: 1184 2257 1184 Size: 1073 5825 BstXI CCAn_nnnn'nTGG Cuts at: 2215 3835 2215 Size: 1620 5278 BstYI r'GATC_y Cuts at: 1972 3574 3947 4228 4595 4980 4997 6449 6535 Size: 1602 373 281 367 385 17 1452 86 Cuts at: 6535 6547 6893 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1602 1452 385 373 367 346 281 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2704 513 Size: 552 1639 4707 Fragments arranged by size: 4707 1639 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6159 Fragments arranged by size: 6159 683 56 BtsI GCAGTG_nn' Cuts at: 2285 3162 4414 5208 5228 2285 Size: 877 1252 794 20 3955 Fragments arranged by size: 3955 1252 877 794 20 ClaI AT'CG_AT Cuts at: 1554 3781 1554 Size: 2227 4671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6863 DraI TTT'AAA Cuts at: 2082 4202 2082 Size: 2120 4778 DraIII CAC_nnn'GTG Cuts at: 2995 2995 Size: 6898 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5905 809 Size: 507 4589 1802 Fragments arranged by size: 4589 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2282 3063 3796 Size: 54 1425 50 275 18 452 781 733 Cuts at: 3796 4345 5234 6863 8 Size: 549 889 1629 43 Fragments arranged by size: 1629 1425 889 781 733 549 452 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4345 1537 Size: 275 2533 4090 Fragments arranged by size: 4090 2533 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3552 3882 4828 1353 Size: 2199 330 946 3423 Fragments arranged by size: 3423 2199 946 330 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2076 5485 5859 6005 387 Size: 1064 138 487 3409 374 146 1280 Fragments arranged by size: 3409 1280 1064 487 374 146 138 EcoICRI GAG'CTC Cuts at: 2814 2814 Size: 6898 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2419 2548 2757 2880 3549 3738 3952 Size: 393 787 129 209 123 669 189 214 Cuts at: 3952 4961 5558 6345 6613 1239 Size: 1009 597 787 268 1524 Fragments arranged by size: 1524 1009 787 787 669 597 393 268 214 209 189 129 123 EcoO109I rG'GnC_Cy Cuts at: 2293 2350 2724 2293 Size: 57 374 6467 Fragments arranged by size: 6467 374 57 EcoRI G'AATT_C Cuts at: 3197 4207 4585 3197 Size: 1010 378 5510 Fragments arranged by size: 5510 1010 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2984 2984 Size: 6898 FspI TGC'GCA Cuts at: 2534 5404 2534 Size: 2870 4028 FspAI rTGC'GCAy Cuts at: 2534 2534 Size: 6898 HaeII r_GCGC'y Cuts at: 1678 2504 2526 2794 2935 2954 6045 1678 Size: 826 22 268 141 19 3091 2531 Fragments arranged by size: 3091 2531 826 268 141 22 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3664 3696 3809 3841 3890 Size: 32 135 32 2092 32 113 32 49 Cuts at: 3890 3922 5539 5571 5613 5645 1373 Size: 32 1617 32 42 32 2626 Fragments arranged by size: 2626 2092 1617 135 113 49 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4242 4488 669 Size: 988 281 2304 246 3079 Fragments arranged by size: 3079 2304 988 281 246 HindIII A'AGCT_T Cuts at: 748 4161 748 Size: 3413 3485 HpaI GTT'AAC Cuts at: 4488 4488 Size: 6898 KasI G'GCGC_C Cuts at: 2522 2522 Size: 6898 KpnI G_GTAC'C Cuts at: 4223 4223 Size: 6898 MfeI C'AATT_G Cuts at: 4497 4497 Size: 6898 MluI A'CGCG_T Cuts at: 6774 6774 Size: 6898 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2709 2841 3483 6012 6196 1305 Size: 1404 132 642 2529 184 2007 Fragments arranged by size: 2529 2007 1404 642 184 132 MscI TGG'CCA Cuts at: 10 64 1489 1832 2284 3065 3798 10 Size: 54 1425 343 452 781 733 3110 Fragments arranged by size: 3110 1425 781 733 452 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2267 2412 2984 3833 3845 4856 Size: 648 1047 54 145 572 849 12 1011 Cuts at: 4856 5215 5374 518 Size: 359 159 2042 Fragments arranged by size: 2042 1047 1011 849 648 572 359 159 145 54 12 MspA1I CmG'CkG Cuts at: 1606 2509 2846 2852 4314 4991 6139 6384 1606 Size: 903 337 6 1462 677 1148 245 2120 Fragments arranged by size: 2120 1462 1148 903 677 337 245 6 NarI GG'CG_CC Cuts at: 2523 2523 Size: 6898 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6346 NdeI CA'TA_TG Cuts at: 387 3151 387 Size: 2764 4134 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6898 NotI GC'GGCC_GC Cuts at: 4345 4345 Size: 6898 NspI r_CATG'y Cuts at: 1132 1229 2127 4256 5801 1132 Size: 97 898 2129 1545 2229 Fragments arranged by size: 2229 2129 1545 898 97 PasI CC'CwG_GG Cuts at: 1348 2541 1348 Size: 1193 5705 PciI A'CATG_T Cuts at: 2123 5797 2123 Size: 3674 3224 PflMI CCAn_nnn'nTGG Cuts at: 1108 3102 1108 Size: 1994 4904 PfoI T'CCnGG_A Cuts at: 3968 3968 Size: 6898 PmeI GTTT'AAAC Cuts at: 4202 4202 Size: 6898 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4967 4999 6480 6512 4967 Size: 32 1481 32 5353 Fragments arranged by size: 5353 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2724 2293 Size: 431 6467 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6898 PsiI TTA'TAA Cuts at: 3234 4468 3234 Size: 1234 5664 PstI C_TGCA'G Cuts at: 830 2870 3140 4250 830 Size: 2040 270 1110 3478 Fragments arranged by size: 3478 2040 1110 270 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6898 RsrII CG'GwC_CG Cuts at: 2831 2831 Size: 6898 SacI G_AGCT'C Cuts at: 2816 2816 Size: 6898 SalI G'TCGA_C Cuts at: 1936 4240 1936 Size: 2304 4594 SanDI GG'GwC_CC Cuts at: 2724 2724 Size: 6898 SbfI CC_TGCA'GG Cuts at: 2870 4250 2870 Size: 1380 5518 ScaI AGT'ACT Cuts at: 5146 6858 5146 Size: 1712 5186 SfcI C'TryA_G Cuts at: 826 1045 2866 3136 3693 4246 5381 6062 6253 Size: 219 1821 270 557 553 1135 681 191 Cuts at: 6253 826 Size: 1471 Fragments arranged by size: 1821 1471 1135 681 557 553 270 219 191 SfoI GGC'GCC Cuts at: 2524 2524 Size: 6898 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6898 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6898 SmaI CCC'GGG Cuts at: 1872 4225 1872 Size: 2353 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5002 5903 6165 820 Size: 197 926 3059 901 262 1553 Fragments arranged by size: 3059 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2575 4689 493 Size: 2082 2114 2702 Fragments arranged by size: 2702 2114 2082 SpeI A'CTAG_T Cuts at: 152 152 Size: 6898 SphI G_CATG'C Cuts at: 1132 4256 1132 Size: 3124 3774 SspI AAT'ATT Cuts at: 5 52 4822 5 Size: 47 4770 2081 Fragments arranged by size: 4770 2081 47 StuI AGG'CCT Cuts at: 1517 3893 1517 Size: 2376 4522 StyI C'CwwG_G Cuts at: 513 1065 2145 3030 3894 4000 4337 513 Size: 552 1080 885 864 106 337 3074 Fragments arranged by size: 3074 1080 885 864 552 337 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4925 4942 5095 5280 5725 325 Size: 1255 3345 17 153 185 445 1498 Fragments arranged by size: 3345 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5144 6856 96 Size: 274 80 33 51 1443 3167 1712 138 Fragments arranged by size: 3167 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2604 2636 4027 4059 6858 6890 2604 Size: 32 1391 32 2799 32 2612 Fragments arranged by size: 2799 2612 1391 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3676 1316 Size: 2360 4538 XbaI T'CTAG_A Cuts at: 4234 4234 Size: 6898 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3116 3221 3385 3116 Size: 105 164 6629 Fragments arranged by size: 6629 164 105 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6898 XmaI C'CCGG_G Cuts at: 1870 4223 1870 Size: 2353 4545 XmnI GAAnn'nnTTC Cuts at: 2933 5027 2933 Size: 2094 4804 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4706 276 Size: 53 83 186 936 3172 2468 Fragments arranged by size: 3172 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII SacII SapI SexAI SfiI SrfI SwaI