(Linear) (Six-base) MAPSORT of: KIEE9458.seq Check: 3047 from: 1 to: 2193 KIEE9458.orf With 182 enzymes: SgfI * March 22, 2012 18:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 875 1544 2193 Size: 875 669 649 Fragments arranged by size: 875 669 649 AfeI AGC'GCT Cuts at: 0 928 947 2193 Size: 928 19 1246 Fragments arranged by size: 1246 928 19 AflIII A'CryG_T Cuts at: 0 118 2193 Size: 118 2075 AhdI GACnn_n'nnGTC Cuts at: 0 1899 2193 Size: 1899 294 AleI CACnn'nnGTG Cuts at: 0 407 1828 1840 2193 Size: 407 1421 12 353 Fragments arranged by size: 1421 407 353 12 ApaLI G'TGCA_C Cuts at: 0 312 2193 Size: 312 1881 ApoI r'AATT_y Cuts at: 0 692 1192 1266 1868 2193 Size: 692 500 74 602 325 Fragments arranged by size: 692 602 500 325 74 AseI AT'TA_AT Cuts at: 0 2054 2193 Size: 2054 139 AvaI C'yCGr_G Cuts at: 0 774 2193 Size: 774 1419 AvrII C'CTAG_G Cuts at: 0 1995 2193 Size: 1995 198 BanI G'GyrC_C Cuts at: 0 517 892 2193 Size: 517 375 1301 Fragments arranged by size: 1301 517 375 BanII G_rGCy'C Cuts at: 0 811 2193 Size: 811 1382 BbeI G_GCGC'C Cuts at: 0 521 2193 Size: 521 1672 BbsI GAAGACnn'nnnn_ Cuts at: 0 1545 2193 Size: 1545 648 BglI GCCn_nnn'nGGC Cuts at: 0 850 2193 Size: 850 1343 BglII A'GATC_T Cuts at: 0 1569 1942 2193 Size: 1569 373 251 Fragments arranged by size: 1569 373 251 Bme1580I G_kGCm'C Cuts at: 0 316 2193 Size: 316 1877 BmrI ACTGGGnnnn_n' Cuts at: 0 1069 1590 2193 Size: 1069 521 603 Fragments arranged by size: 1069 603 521 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 414 543 752 1733 1947 2193 Size: 414 129 209 981 214 246 Fragments arranged by size: 981 414 246 214 209 129 BsaAI yAC'GTr Cuts at: 0 570 2193 Size: 570 1623 BsaBI GATnn'nnATC Cuts at: 0 1947 2193 Size: 1947 246 BsaHI Gr'CG_yC Cuts at: 0 518 1156 2193 Size: 518 638 1037 Fragments arranged by size: 1037 638 518 BsaWI w'CCGG_w Cuts at: 0 1627 2193 Size: 1627 566 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 601 673 2193 Size: 601 72 1520 Fragments arranged by size: 1520 601 72 BseYI C'CCAG_C Cuts at: 0 512 847 2193 Size: 512 335 1346 Fragments arranged by size: 1346 512 335 BsiHKAI G_wGCw'C Cuts at: 0 316 452 811 2193 Size: 316 136 359 1382 Fragments arranged by size: 1382 359 316 136 BsmI GAATG_Cn' Cuts at: 0 1657 2193 Size: 1657 536 Bsp1286I G_dGCh'C Cuts at: 0 316 452 811 2193 Size: 316 136 359 1382 Fragments arranged by size: 1382 359 316 136 BspHI T'CATG_A Cuts at: 0 125 236 1548 2193 Size: 125 111 1312 645 Fragments arranged by size: 1312 645 125 111 BssSI C'ACGA_G Cuts at: 0 613 2193 Size: 613 1580 BstEII G'GTnAC_C Cuts at: 0 252 2193 Size: 252 1941 BstXI CCAn_nnnn'nTGG Cuts at: 0 210 1830 2193 Size: 210 1620 363 Fragments arranged by size: 1620 363 210 BstYI r'GATC_y Cuts at: 0 1569 1942 2193 Size: 1569 373 251 Fragments arranged by size: 1569 373 251 BtgI C'CryG_G Cuts at: 0 699 2193 Size: 699 1494 BtsI GCAGTG_nn' Cuts at: 0 280 1157 2193 Size: 280 877 1036 Fragments arranged by size: 1036 877 280 ClaI AT'CG_AT Cuts at: 0 1776 2193 Size: 1776 417 DraI TTT'AAA Cuts at: 0 77 2193 Size: 77 2116 DraIII CAC_nnn'GTG Cuts at: 0 990 2193 Size: 990 1203 EaeI y'GGCC_r Cuts at: 0 277 1058 1791 2193 Size: 277 781 733 402 Fragments arranged by size: 781 733 402 277 EarI CTCTTCn'nnn_ Cuts at: 0 1547 1877 2193 Size: 1547 330 316 Fragments arranged by size: 1547 330 316 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 71 2193 Size: 71 2122 EcoICRI GAG'CTC Cuts at: 0 809 2193 Size: 809 1384 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 414 543 752 875 1544 1733 1947 2193 Size: 414 129 209 123 669 189 214 246 Fragments arranged by size: 669 414 246 214 209 189 129 123 EcoO109I rG'GnC_Cy Cuts at: 0 288 345 719 2193 Size: 288 57 374 1474 Fragments arranged by size: 1474 374 288 57 EcoRI G'AATT_C Cuts at: 0 1192 2193 Size: 1192 1001 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 979 2193 Size: 979 1214 FspI TGC'GCA Cuts at: 0 529 2193 Size: 529 1664 FspAI rTGC'GCAy Cuts at: 0 529 2193 Size: 529 1664 HaeII r_GCGC'y Cuts at: 0 499 521 789 930 949 2193 Size: 499 22 268 141 19 1244 Fragments arranged by size: 1244 499 268 141 22 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1659 1691 1804 1836 1885 1917 2193 Size: 1659 32 113 32 49 32 276 Fragments arranged by size: 1659 276 113 49 32 32 32 HindIII A'AGCT_T Cuts at: 0 2156 2193 Size: 2156 37 KasI G'GCGC_C Cuts at: 0 517 2193 Size: 517 1676 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 704 836 1478 2193 Size: 704 132 642 715 Fragments arranged by size: 715 704 642 132 MscI TGG'CCA Cuts at: 0 279 1060 1793 2193 Size: 279 781 733 400 Fragments arranged by size: 781 733 400 279 MslI CAynn'nnrTG Cuts at: 0 208 262 407 979 1828 1840 2193 Size: 208 54 145 572 849 12 353 Fragments arranged by size: 849 572 353 208 145 54 12 MspA1I CmG'CkG Cuts at: 0 504 841 847 2193 Size: 504 337 6 1346 Fragments arranged by size: 1346 504 337 6 NarI GG'CG_CC Cuts at: 0 518 2193 Size: 518 1675 NdeI CA'TA_TG Cuts at: 0 1146 2193 Size: 1146 1047 NspI r_CATG'y Cuts at: 0 122 2193 Size: 122 2071 PasI CC'CwG_GG Cuts at: 0 536 2193 Size: 536 1657 PciI A'CATG_T Cuts at: 0 118 2193 Size: 118 2075 PflMI CCAn_nnn'nTGG Cuts at: 0 1097 2193 Size: 1097 1096 PfoI T'CCnGG_A Cuts at: 0 1963 2193 Size: 1963 230 PpuMI rG'GwC_Cy Cuts at: 0 288 719 2193 Size: 288 431 1474 Fragments arranged by size: 1474 431 288 PsiI TTA'TAA Cuts at: 0 1229 2193 Size: 1229 964 PstI C_TGCA'G Cuts at: 0 865 1135 2193 Size: 865 270 1058 Fragments arranged by size: 1058 865 270 RsrII CG'GwC_CG Cuts at: 0 826 2193 Size: 826 1367 SacI G_AGCT'C Cuts at: 0 811 2193 Size: 811 1382 SanDI GG'GwC_CC Cuts at: 0 719 2193 Size: 719 1474 SbfI CC_TGCA'GG Cuts at: 0 865 2193 Size: 865 1328 SfcI C'TryA_G Cuts at: 0 861 1131 1688 2193 Size: 861 270 557 505 Fragments arranged by size: 861 557 505 270 SfoI GGC'GCC Cuts at: 0 519 2193 Size: 519 1674 SnaBI TAC'GTA Cuts at: 0 570 2193 Size: 570 1623 StuI AGG'CCT Cuts at: 0 1888 2193 Size: 1888 305 StyI C'CwwG_G Cuts at: 0 140 1025 1889 1995 2193 Size: 140 885 864 106 198 Fragments arranged by size: 885 864 198 140 106 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 599 631 2022 2054 2193 Size: 599 32 1391 32 139 Fragments arranged by size: 1391 599 139 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1671 2193 Size: 1671 522 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1111 1216 1380 2193 Size: 1111 105 164 813 Fragments arranged by size: 1111 813 164 105 XmnI GAAnn'nnTTC Cuts at: 0 928 2193 Size: 928 1265 Enzymes that do cut: AcuI AfeI AflIII AhdI AleI ApaLI ApoI AseI AvaI AvrII BanI BanII BbeI BbsI BglI BglII Bme1580I BmrI BpmI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BssSI BstEII BstXI BstYI BtgI BtsI ClaI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NarI NdeI NspI PasI PciI PflMI PfoI PpuMI PsiI PstI RsrII SacI SanDI SbfI SfcI SfoI SnaBI StuI StyI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AlfI AloI AlwNI ApaI AscI AsiSI BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI CspCI DrdI EagI EcoNI EcoRV FseI HincII HpaI KpnI MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI PvuII SacII SalI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI SwaI TaqII TatI XbaI XhoI XmaI ZraI