################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccII AccIII AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrBI BssHII BstBI BstUI CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MunI MvnI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AclI 1141 AACGTT AA^CGTT AfaI 834 GTAC GT^AC AflIII 1772 ACATGT A^C[GA][CT]GT AlwNI 547 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG BciVI 913 GTATCC GTATCC(6/5) BclI 149 TGATCA T^GATCA BglII 1553 AGATCT A^GATCT BsaBI 150 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1946 TCCGGT [AT]^CCGG[AT] Bsp1407I 833 TGTACA T^GTACA BspLU11I 1772 ACATGT A^CATGT BsrDI 1011 CATTGC GCAATG(2/0) BsrGI 833 TGTACA T^GTACA Bst1107I 1845 GTATAC GTA^TAC BstAPI 2124 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1107 GGTGACC G^GT[ACGT]ACC BstPI 1107 GGTGACC G^GT[ACGT]ACC BstXI 1376 CCAAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1845 GTATAC GTA^TAC DrdI 3 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1107 GGTGACC G^GT[ACGT]ACC EcoRI 1369 GAATTC G^AATTC FbaI 149 TGATCA T^GATCA HindIII 1411 AAGCTT A^AGCTT HpaI 1855 GTTAAC GTT^AAC MamI 150 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 745 GCCGGC GCC^GGC NcoI 1112 CCATGG C^CATGG NdeI 1649 CATATG CA^TATG NgoMIV 745 GCCGGC G^CCGGC PaeR7I 872 CTCGAG C^TCGAG PflMI 619 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1141 AACGTT AA^CGTT RsaI 834 GTAC GT^AC SbfI 1976 CCTGCAGG CCTGCA^GG SmaI 684 CCCGGG CCC^GGG Sse8387I 1976 CCTGCAGG CCTGCA^GG SspBI 833 TGTACA T^GTACA StuI 1275 AGGCCT AGG^CCT Van91I 619 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 785 CCAGCATTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 872 CTCGAG C^TCGAG XmaCI 684 CCCGGG C^CCGGG XmaI 684 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 316 GTCTAC GT^[AC][GT]AC 1845 GTATAC AcsI 476 GAATTT [GA]^AATT[CT] 1369 GAATTC ApoI 476 GAATTT [GA]^AATT[CT] 1369 GAATTC BbeI 859 GGCGCC GGCGC^C 2016 GGCGCC BsaI 1788 GGTCTC GGTCTC(1/5) 1958 GGTCTC BsmAI 1789 GTCTC GTCTC(1/5) 1959 GTCTC BsmI 2067 GAATGC GAATGC(1/-1) 788 GCATTC BssSI 822 CTCGTG CACGAG(-5/-1) 2110 CTCGTG BstYI 1553 AGATCT [GA]^GATC[CT] 1750 AGATCC DsaI 1079 CCGTGG C^C[GA][CT]GG 1112 CCATGG EcoNI 1973 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2025 CCTGGCTAAGG FauI 995 CCCGC CCCGC(4/6) 1570 CCCGC HaeII 859 GGCGCC [GA]GCGC^[CT] 2016 GGCGCC HgaI 281 GCGTC GACGC(5/10) 314 GCGTC HincII 1855 GTTAAC GT[CT]^[GA]AC 2035 GTTGAC HindII 1855 GTTAAC GT[CT]^[GA]AC 2035 GTTGAC KasI 859 GGCGCC G^GCGCC 2016 GGCGCC MaeII 1142 ACGT A^CGT 1184 ACGT MflI 1553 AGATCT [GA]^GATC[CT] 1750 AGATCC MseI 512 TTAA T^TAA 1856 TTAA NarI 859 GGCGCC GG^CGCC 2016 GGCGCC NspI 221 GCATGT [GA]CATG^[CT] 1772 ACATGT SacI 25 GAGCTC GAGCT^C 989 GAGCTC SapI 27 GCTCTTC GCTCTTC(1/4) 171 GCTCTTC SfoI 859 GGCGCC GGC^GCC 2016 GGCGCC SmlI 872 CTCGAG C^T[CT][GA]AG 1414 CTTGAG TaqI 873 TCGA T^CGA 1550 TCGA TfiI 427 GAATC G^A[AT]TC 691 GAATC Tru9I 512 TTAA T^TAA 1856 TTAA Tsp509I 477 AATT ^AATT 1370 AATT TthHB8I 873 TCGA T^CGA 1550 TCGA XhoII 1553 AGATCT [GA]^GATC[CT] 1750 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcyI AluI AlwI AocI AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI