(Circular) (Six-base) MAPSORT of: pFN21AE6754.seq Check: 962 from: 1 to: 6847 pFN21AE6754 6847 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2393 2516 3309 2393 Size: 123 793 5931 Fragments arranged by size: 5931 793 123 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4657 278 Size: 53 83 186 936 3121 2468 Fragments arranged by size: 3121 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2322 3851 4190 1937 Size: 385 1529 339 4594 Fragments arranged by size: 4594 1529 385 339 Acc65I G'GTAC_C Cuts at: 4168 4168 Size: 6847 AclI AA'CG_TT Cuts at: 1563 3147 4974 5347 1563 Size: 1584 1827 373 3063 Fragments arranged by size: 3063 1827 1584 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3740 3925 4910 6294 6562 1632 Size: 2108 185 985 1384 268 1917 Fragments arranged by size: 2108 1917 1384 985 268 185 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6650 AflIII A'CryG_T Cuts at: 1203 3777 5746 6723 1203 Size: 2574 1969 977 1327 Fragments arranged by size: 2574 1969 1327 977 AgeI A'CCGG_T Cuts at: 5678 5678 Size: 6847 AhdI GACnn_n'nnGTC Cuts at: 5576 5576 Size: 6847 AleI CACnn'nnGTG Cuts at: 1166 2213 1166 Size: 1047 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3033 4007 1184 Size: 1849 974 4024 Fragments arranged by size: 4024 1849 974 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2123 2155 2123 Size: 32 6815 AlwNI CAG_nnn'CTG Cuts at: 2557 4590 6162 2557 Size: 2033 1572 3242 Fragments arranged by size: 3242 2033 1572 ApaLI G'TGCA_C Cuts at: 4904 6060 4904 Size: 1156 5691 ApoI r'AATT_y Cuts at: 1493 2481 3374 4156 4382 4534 6643 1493 Size: 988 893 782 226 152 2109 1697 Fragments arranged by size: 2109 1697 988 893 782 226 152 AseI AT'TA_AT Cuts at: 160 1032 5401 160 Size: 872 4369 1606 Fragments arranged by size: 4369 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6847 AvaI C'yCGr_G Cuts at: 1870 1943 2456 2689 2877 4089 4172 1870 Size: 73 513 233 188 1212 83 4545 Fragments arranged by size: 4545 1212 513 233 188 83 73 BamHI G'GATC_C Cuts at: 4177 4544 4177 Size: 367 6480 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 2405 2864 3247 4021 Size: 325 217 627 576 42 459 383 774 Cuts at: 4021 4168 5623 618 Size: 147 1455 1842 Fragments arranged by size: 1842 1455 774 627 576 459 383 325 217 147 42 BanII G_rGCy'C Cuts at: 1391 2034 2807 2998 4166 1391 Size: 643 773 191 1168 4072 Fragments arranged by size: 4072 1168 773 643 191 BbeI G_GCGC'C Cuts at: 2868 4025 2868 Size: 1157 5690 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2093 2839 2845 928 Size: 971 194 746 6 4930 Fragments arranged by size: 4930 971 746 194 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2269 2287 2303 2321 2503 2537 5038 Size: 34 332 18 16 18 182 34 2501 Cuts at: 5038 5072 1903 Size: 34 3678 Fragments arranged by size: 3678 2501 332 182 34 34 34 18 18 16 BciVI GTATCCnnnnn_n' Cuts at: 2929 4739 5949 2929 Size: 1810 1210 3827 Fragments arranged by size: 3827 1810 1210 BclI T'GATC_A Cuts at: 1548 2154 1548 Size: 606 6241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5458 136 Size: 107 122 71 631 605 3786 1525 Fragments arranged by size: 3786 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3558 6842 3558 Size: 3284 3563 BlpI GC'TnA_GC Cuts at: 4264 4264 Size: 6847 Bme1580I G_kGCm'C Cuts at: 1790 3365 4908 6064 1790 Size: 1575 1543 1156 2573 Fragments arranged by size: 2573 1575 1543 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6847 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3315 4578 5536 Size: 416 53 83 399 24 1898 1263 958 Cuts at: 5536 442 Size: 1753 Fragments arranged by size: 1898 1753 1263 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6847 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2534 3386 3769 5507 1239 Size: 1295 852 383 1738 2579 Fragments arranged by size: 2579 1738 1295 852 383 Bpu10I CC'TnA_GC Cuts at: 3252 3263 3675 4035 3252 Size: 11 412 360 6064 Fragments arranged by size: 6064 412 360 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3440 4972 5837 6135 3440 Size: 1532 865 298 4152 Fragments arranged by size: 4152 1532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3799 3969 5510 882 Size: 617 2300 170 1541 2219 Fragments arranged by size: 2300 2219 1541 617 170 BsaAI yAC'GTr Cuts at: 493 1204 4638 493 Size: 711 3434 2702 Fragments arranged by size: 3434 2702 711 BsaBI GATnn'nnATC Cuts at: 2159 6841 2159 Size: 4682 2165 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2286 2319 2865 4022 Size: 53 83 186 936 753 33 546 1157 Cuts at: 4022 4654 5036 5671 275 Size: 632 382 635 1451 Fragments arranged by size: 1451 1157 936 753 635 632 546 382 186 83 53 33 BsaWI w'CCGG_w Cuts at: 3951 5280 5678 5952 6099 6510 3951 Size: 1329 398 274 147 411 4288 Fragments arranged by size: 4288 1329 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4091 4094 4121 4124 1178 Size: 30 165 30 2688 3 27 3 3901 Fragments arranged by size: 3901 2688 165 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2326 3389 4099 1186 Size: 1140 1063 710 3934 Fragments arranged by size: 3934 1140 1063 710 BseYI C'CCAG_C Cuts at: 2049 2067 2073 2091 2449 2509 2728 6050 6546 Size: 18 6 18 358 60 219 3322 496 Cuts at: 6546 2049 Size: 2350 Fragments arranged by size: 3322 2350 496 358 219 60 18 18 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2045 2857 3024 1163 Size: 573 309 812 167 4986 Fragments arranged by size: 4986 812 573 309 167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4297 5058 6086 1540 Size: 275 186 2296 761 1028 2301 Fragments arranged by size: 2301 2296 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2178 2220 2998 3264 3300 4908 4993 6064 Size: 144 42 778 266 36 1608 85 1071 Cuts at: 6064 2034 Size: 2817 Fragments arranged by size: 2817 1608 1071 778 266 144 85 42 36 BsmI GAATG_Cn' Cuts at: 2793 4078 4358 4451 2793 Size: 1285 280 93 5189 Fragments arranged by size: 5189 1285 280 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2178 2220 2807 2998 3264 3300 Size: 399 244 144 42 587 191 266 36 Cuts at: 3300 3365 4166 4908 4993 6064 1391 Size: 65 801 742 85 1071 2174 Fragments arranged by size: 2174 1071 801 742 587 399 266 244 191 144 85 65 42 36 BspEI T'CCGG_A Cuts at: 6510 6510 Size: 6847 BspHI T'CATG_A Cuts at: 4736 6466 4736 Size: 1730 5117 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2393 2516 3309 3349 4202 844 Size: 1549 123 793 40 853 3489 Fragments arranged by size: 3489 1549 853 793 123 40 BsrBI CCG'CTC Cuts at: 1394 1823 4734 1394 Size: 429 2911 3507 Fragments arranged by size: 3507 2911 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3015 5342 5516 65 Size: 1183 1767 2327 174 1396 Fragments arranged by size: 2327 1767 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2420 2750 3256 5491 5678 1700 Size: 110 610 330 506 2235 187 2869 Fragments arranged by size: 2869 2235 610 506 330 187 110 BsrGI T'GTAC_A Cuts at: 96 2838 96 Size: 2742 4105 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6847 BssSI C'ACGA_G Cuts at: 2827 4115 4907 5919 2827 Size: 1288 792 1012 3755 Fragments arranged by size: 3755 1288 1012 792 BstAPI GCAn_nnn'nTGC Cuts at: 794 4135 794 Size: 3341 3506 BstBI TT'CG_AA Cuts at: 5651 5651 Size: 6847 BstEII G'GTnAC_C Cuts at: 1184 3112 1184 Size: 1928 4919 BstXI CCAn_nnnn'nTGG Cuts at: 3388 3388 Size: 6847 BstYI r'GATC_y Cuts at: 1972 3558 3755 4177 4544 4929 4946 6398 6484 Size: 1586 197 422 367 385 17 1452 86 Cuts at: 6484 6496 6842 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1586 1452 422 385 367 346 197 86 17 12 BstZ17I GTA'TAC Cuts at: 3852 3852 Size: 6847 Bsu36I CC'TnA_GG Cuts at: 2183 2774 3645 2183 Size: 591 871 5385 Fragments arranged by size: 5385 871 591 BtgI C'CryG_G Cuts at: 513 1065 3084 3117 513 Size: 552 2019 33 4243 Fragments arranged by size: 4243 2019 552 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6108 Fragments arranged by size: 6108 683 56 BtsI GCAGTG_nn' Cuts at: 3017 3201 3286 4363 5157 5177 3017 Size: 184 85 1077 794 20 4687 Fragments arranged by size: 4687 1077 794 184 85 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6847 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6812 DraI TTT'AAA Cuts at: 4151 4151 Size: 6847 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2014 5854 809 Size: 507 698 3840 1802 Fragments arranged by size: 3840 1802 698 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2574 2748 3898 Size: 54 1425 50 275 18 744 174 1150 Cuts at: 3898 4294 5183 6812 8 Size: 396 889 1629 43 Fragments arranged by size: 1629 1425 1150 889 744 396 275 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4294 1537 Size: 275 2482 4090 Fragments arranged by size: 4090 2482 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 2183 3467 4777 1353 Size: 686 144 1284 1310 3423 Fragments arranged by size: 3423 1310 1284 686 144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2534 5434 5808 5954 387 Size: 1064 138 945 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 945 374 146 138 EcoICRI GAG'CTC Cuts at: 2032 2996 2032 Size: 964 5883 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2534 3386 3740 3769 3925 4910 5507 Size: 393 902 852 354 29 156 985 597 Cuts at: 5507 6294 6562 1239 Size: 787 268 1524 Fragments arranged by size: 1524 985 902 852 787 597 393 354 268 156 29 EcoNI CCTnn'n_nnAGG Cuts at: 3982 4034 3982 Size: 52 6795 EcoO109I rG'GnC_Cy Cuts at: 2124 2884 3338 3456 3816 2124 Size: 760 454 118 360 5155 Fragments arranged by size: 5155 760 454 360 118 EcoRI G'AATT_C Cuts at: 3374 4156 4534 3374 Size: 782 378 5687 Fragments arranged by size: 5687 782 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3777 3777 Size: 6847 FspI TGC'GCA Cuts at: 5353 5353 Size: 6847 HaeII r_GCGC'y Cuts at: 1678 2868 4025 5994 1678 Size: 1190 1157 1969 2531 Fragments arranged by size: 2531 1969 1190 1157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2352 2384 3518 3550 3949 Size: 32 135 32 780 32 1134 32 399 Cuts at: 3949 3981 5488 5520 5562 5594 1373 Size: 32 1507 32 42 32 2626 Fragments arranged by size: 2626 1507 1134 780 399 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3862 4042 4191 4437 669 Size: 988 281 1924 180 149 246 3079 Fragments arranged by size: 3079 1924 988 281 246 180 149 HindIII A'AGCT_T Cuts at: 748 3416 748 Size: 2668 4179 HpaI GTT'AAC Cuts at: 3862 4437 3862 Size: 575 6272 KasI G'GCGC_C Cuts at: 2864 4021 2864 Size: 1157 5690 KpnI G_GTAC'C Cuts at: 4172 4172 Size: 6847 MfeI C'AATT_G Cuts at: 4446 4446 Size: 6847 MluI A'CGCG_T Cuts at: 6723 6723 Size: 6847 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3960 5961 6145 1305 Size: 2655 2001 184 2007 Fragments arranged by size: 2655 2007 2001 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5025 Fragments arranged by size: 5025 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2252 3833 4049 4805 5164 5323 Size: 648 1047 39 1581 216 756 359 159 Cuts at: 5323 518 Size: 2042 Fragments arranged by size: 2042 1581 1047 756 648 359 216 159 39 MspA1I CmG'CkG Cuts at: 1606 2095 2313 2554 2732 2816 3578 4132 4263 Size: 489 218 241 178 84 762 554 131 Cuts at: 4263 4940 6088 6333 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 762 677 554 489 245 241 218 178 131 84 NaeI GCC'GGC Cuts at: 2752 2752 Size: 6847 NarI GG'CG_CC Cuts at: 2865 4022 2865 Size: 1157 5690 NcoI C'CATG_G Cuts at: 513 1065 3117 513 Size: 552 2052 4243 Fragments arranged by size: 4243 2052 552 NdeI CA'TA_TG Cuts at: 387 3655 387 Size: 3268 3579 NgoMIV G'CCGG_C Cuts at: 2750 2750 Size: 6847 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6847 NotI GC'GGCC_GC Cuts at: 4294 4294 Size: 6847 NspI r_CATG'y Cuts at: 1132 1229 2230 3781 4205 5750 1132 Size: 97 1001 1551 424 1545 2229 Fragments arranged by size: 2229 1551 1545 1001 424 97 PasI CC'CwG_GG Cuts at: 1348 2800 3789 1348 Size: 1452 989 4406 Fragments arranged by size: 4406 1452 989 PciI A'CATG_T Cuts at: 3777 5746 3777 Size: 1969 4878 PflMI CCAn_nnn'nTGG Cuts at: 1108 2630 1108 Size: 1522 5325 PmeI GTTT'AAAC Cuts at: 4151 4151 Size: 6847 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4916 4948 6429 6461 4916 Size: 32 1481 32 5302 Fragments arranged by size: 5302 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2124 3338 3456 3816 2124 Size: 1214 118 360 5155 Fragments arranged by size: 5155 1214 360 118 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6847 PsiI TTA'TAA Cuts at: 4417 4417 Size: 6847 PstI C_TGCA'G Cuts at: 830 3012 3236 3986 4199 830 Size: 2182 224 750 213 3478 Fragments arranged by size: 3478 2182 750 224 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6847 PvuII CAG'CTG Cuts at: 2095 2554 2816 4132 2095 Size: 459 262 1316 4810 Fragments arranged by size: 4810 1316 459 262 SacI G_AGCT'C Cuts at: 2034 2998 2034 Size: 964 5883 SalI G'TCGA_C Cuts at: 1936 4189 1936 Size: 2253 4594 SanDI GG'GwC_CC Cuts at: 3816 3816 Size: 6847 SapI GCTCTTCn'nnn_ Cuts at: 2039 2183 2039 Size: 144 6703 SbfI CC_TGCA'GG Cuts at: 3986 4199 3986 Size: 213 6634 ScaI AGT'ACT Cuts at: 5095 6807 5095 Size: 1712 5135 SfcI C'TryA_G Cuts at: 826 1045 2660 2736 3008 3232 3982 4195 5330 Size: 219 1615 76 272 224 750 213 1135 Cuts at: 5330 6011 6202 826 Size: 681 191 1471 Fragments arranged by size: 1615 1471 1135 750 681 272 224 219 213 191 76 SfoI GGC'GCC Cuts at: 2866 4023 2866 Size: 1157 5690 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6847 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6847 SmaI CCC'GGG Cuts at: 1872 2691 4174 1872 Size: 819 1483 4545 Fragments arranged by size: 4545 1483 819 SmlI C'TyrA_G Cuts at: 820 1017 1943 2877 3419 4951 5852 6114 820 Size: 197 926 934 542 1532 901 262 1553 Fragments arranged by size: 1553 1532 934 926 901 542 262 197 SnaBI TAC'GTA Cuts at: 493 4638 493 Size: 4145 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6847 SphI G_CATG'C Cuts at: 1132 4205 1132 Size: 3073 3774 SspI AAT'ATT Cuts at: 5 52 4771 5 Size: 47 4719 2081 Fragments arranged by size: 4719 2081 47 StuI AGG'CCT Cuts at: 1517 3282 1517 Size: 1765 5082 StyI C'CwwG_G Cuts at: 513 1065 3117 3381 3922 4286 513 Size: 552 2052 264 541 364 3074 Fragments arranged by size: 3074 2052 552 541 364 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2329 2336 2627 3835 3857 4079 4874 Size: 1255 749 7 291 1208 22 222 795 Cuts at: 4874 4891 5044 5229 5674 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1208 795 749 445 291 222 185 153 22 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2838 5093 6805 Size: 274 80 33 51 1443 861 2255 1712 Cuts at: 6805 96 Size: 138 Fragments arranged by size: 2255 1712 1443 861 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4094 4126 6807 6839 4094 Size: 32 2681 32 4102 Fragments arranged by size: 4102 2681 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6847 XbaI T'CTAG_A Cuts at: 4183 4183 Size: 6847 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2797 2797 Size: 6847 XhoI C'TCGA_G Cuts at: 1943 2877 1943 Size: 934 5913 XmaI C'CCGG_G Cuts at: 1870 2689 4172 1870 Size: 819 1483 4545 Fragments arranged by size: 4545 1483 819 XmnI GAAnn'nnTTC Cuts at: 4976 4976 Size: 6847 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4655 276 Size: 53 83 186 936 3121 2468 Fragments arranged by size: 3121 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI