(Linear) (Six-base) MAPSORT of: KIEE6754.seq Check: 9710 from: 1 to: 2142 KIEE6754.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 388 511 1304 2142 Size: 388 123 793 838 Fragments arranged by size: 838 793 388 123 AccI GT'mk_AC Cuts at: 0 317 1846 2142 Size: 317 1529 296 Fragments arranged by size: 1529 317 296 AclI AA'CG_TT Cuts at: 0 1142 2142 Size: 1142 1000 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1735 1920 2142 Size: 1735 185 222 Fragments arranged by size: 1735 222 185 AflIII A'CryG_T Cuts at: 0 1772 2142 Size: 1772 370 AleI CACnn'nnGTG Cuts at: 0 208 2142 Size: 208 1934 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1028 2002 2142 Size: 1028 974 140 Fragments arranged by size: 1028 974 140 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 118 150 2142 Size: 118 32 1992 Fragments arranged by size: 1992 118 32 AlwNI CAG_nnn'CTG Cuts at: 0 552 2142 Size: 552 1590 ApoI r'AATT_y Cuts at: 0 476 1369 2142 Size: 476 893 773 Fragments arranged by size: 893 773 476 AvaI C'yCGr_G Cuts at: 0 451 684 872 2084 2142 Size: 451 233 188 1212 58 Fragments arranged by size: 1212 451 233 188 58 BanI G'GyrC_C Cuts at: 0 358 400 859 1242 2016 2142 Size: 358 42 459 383 774 126 Fragments arranged by size: 774 459 383 358 126 42 BanII G_rGCy'C Cuts at: 0 29 802 993 2142 Size: 29 773 191 1149 Fragments arranged by size: 1149 773 191 29 BbeI G_GCGC'C Cuts at: 0 863 2020 2142 Size: 863 1157 122 Fragments arranged by size: 1157 863 122 BbsI GAAGACnn'nnnn_ Cuts at: 0 88 834 840 2142 Size: 88 746 6 1302 Fragments arranged by size: 1302 746 88 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 264 282 298 316 498 532 2142 Size: 264 18 16 18 182 34 1610 Fragments arranged by size: 1610 264 182 34 18 18 16 BciVI GTATCCnnnnn_n' Cuts at: 0 924 2142 Size: 924 1218 BclI T'GATC_A Cuts at: 0 149 2142 Size: 149 1993 BglII A'GATC_T Cuts at: 0 1553 2142 Size: 1553 589 Bme1580I G_kGCm'C Cuts at: 0 1360 2142 Size: 1360 782 BmrI ACTGGGnnnn_n' Cuts at: 0 1310 2142 Size: 1310 832 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 529 1381 1764 2142 Size: 529 852 383 378 Fragments arranged by size: 852 529 383 378 Bpu10I CC'TnA_GC Cuts at: 0 1247 1258 1670 2030 2142 Size: 1247 11 412 360 112 Fragments arranged by size: 1247 412 360 112 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1435 2142 Size: 1435 707 BsaI GGTCTCn'nnnn_ Cuts at: 0 1794 1964 2142 Size: 1794 170 178 Fragments arranged by size: 1794 178 170 BsaBI GATnn'nnATC Cuts at: 0 154 2142 Size: 154 1988 BsaHI Gr'CG_yC Cuts at: 0 281 314 860 2017 2142 Size: 281 33 546 1157 125 Fragments arranged by size: 1157 546 281 125 33 BsaWI w'CCGG_w Cuts at: 0 1946 2142 Size: 1946 196 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2086 2089 2116 2119 2142 Size: 2086 3 27 3 23 Fragments arranged by size: 2086 27 23 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 321 1384 2094 2142 Size: 321 1063 710 48 Fragments arranged by size: 1063 710 321 48 BseYI C'CCAG_C Cuts at: 0 44 62 68 86 444 504 723 2142 Size: 44 18 6 18 358 60 219 1419 Fragments arranged by size: 1419 358 219 60 44 18 18 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 852 1019 2142 Size: 40 812 167 1123 Fragments arranged by size: 1123 812 167 40 BsiHKAI G_wGCw'C Cuts at: 0 29 173 215 993 1259 1295 2142 Size: 29 144 42 778 266 36 847 Fragments arranged by size: 847 778 266 144 42 36 29 BsmI GAATG_Cn' Cuts at: 0 788 2073 2142 Size: 788 1285 69 Fragments arranged by size: 1285 788 69 Bsp1286I G_dGCh'C Cuts at: 0 29 173 215 802 993 1259 1295 1360 Size: 29 144 42 587 191 266 36 65 Cuts at: 1360 2142 Size: 782 Fragments arranged by size: 782 587 266 191 144 65 42 36 29 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 388 511 1304 1344 2142 Size: 388 123 793 40 798 Fragments arranged by size: 798 793 388 123 40 BsrDI GCAATG_nn' Cuts at: 0 1010 2142 Size: 1010 1132 BsrFI r'CCGG_y Cuts at: 0 415 745 1251 2142 Size: 415 330 506 891 Fragments arranged by size: 891 506 415 330 BsrGI T'GTAC_A Cuts at: 0 833 2142 Size: 833 1309 BssSI C'ACGA_G Cuts at: 0 822 2110 2142 Size: 822 1288 32 Fragments arranged by size: 1288 822 32 BstAPI GCAn_nnn'nTGC Cuts at: 0 2130 2142 Size: 2130 12 BstEII G'GTnAC_C Cuts at: 0 1107 2142 Size: 1107 1035 BstXI CCAn_nnnn'nTGG Cuts at: 0 1383 2142 Size: 1383 759 BstYI r'GATC_y Cuts at: 0 1553 1750 2142 Size: 1553 197 392 Fragments arranged by size: 1553 392 197 BstZ17I GTA'TAC Cuts at: 0 1847 2142 Size: 1847 295 Bsu36I CC'TnA_GG Cuts at: 0 178 769 1640 2142 Size: 178 591 871 502 Fragments arranged by size: 871 591 502 178 BtgI C'CryG_G Cuts at: 0 1079 1112 2142 Size: 1079 33 1030 Fragments arranged by size: 1079 1030 33 BtsI GCAGTG_nn' Cuts at: 0 1012 1196 1281 2142 Size: 1012 184 85 861 Fragments arranged by size: 1012 861 184 85 DrdI GACnn_nn'nnGTC Cuts at: 0 9 2142 Size: 9 2133 EaeI y'GGCC_r Cuts at: 0 569 743 1893 2142 Size: 569 174 1150 249 Fragments arranged by size: 1150 569 249 174 EarI CTCTTCn'nnn_ Cuts at: 0 34 178 1462 2142 Size: 34 144 1284 680 Fragments arranged by size: 1284 680 144 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 529 2142 Size: 529 1613 EcoICRI GAG'CTC Cuts at: 0 27 991 2142 Size: 27 964 1151 Fragments arranged by size: 1151 964 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 529 1381 1735 1764 1920 2142 Size: 529 852 354 29 156 222 Fragments arranged by size: 852 529 354 222 156 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 1977 2029 2142 Size: 1977 52 113 Fragments arranged by size: 1977 113 52 EcoO109I rG'GnC_Cy Cuts at: 0 119 879 1333 1451 1811 2142 Size: 119 760 454 118 360 331 Fragments arranged by size: 760 454 360 331 119 118 EcoRI G'AATT_C Cuts at: 0 1369 2142 Size: 1369 773 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1772 2142 Size: 1772 370 HaeII r_GCGC'y Cuts at: 0 863 2020 2142 Size: 863 1157 122 Fragments arranged by size: 1157 863 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 347 379 1513 1545 1944 1976 2142 Size: 347 32 1134 32 399 32 166 Fragments arranged by size: 1134 399 347 166 32 32 32 HincII GTy'rAC Cuts at: 0 1857 2037 2142 Size: 1857 180 105 Fragments arranged by size: 1857 180 105 HindIII A'AGCT_T Cuts at: 0 1411 2142 Size: 1411 731 HpaI GTT'AAC Cuts at: 0 1857 2142 Size: 1857 285 KasI G'GCGC_C Cuts at: 0 859 2016 2142 Size: 859 1157 126 Fragments arranged by size: 1157 859 126 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1955 2142 Size: 1955 187 MslI CAynn'nnrTG Cuts at: 0 208 247 1828 2044 2142 Size: 208 39 1581 216 98 Fragments arranged by size: 1581 216 208 98 39 MspA1I CmG'CkG Cuts at: 0 90 308 549 727 811 1573 2127 2142 Size: 90 218 241 178 84 762 554 15 Fragments arranged by size: 762 554 241 218 178 90 84 15 NaeI GCC'GGC Cuts at: 0 747 2142 Size: 747 1395 NarI GG'CG_CC Cuts at: 0 860 2017 2142 Size: 860 1157 125 Fragments arranged by size: 1157 860 125 NcoI C'CATG_G Cuts at: 0 1112 2142 Size: 1112 1030 NdeI CA'TA_TG Cuts at: 0 1650 2142 Size: 1650 492 NgoMIV G'CCGG_C Cuts at: 0 745 2142 Size: 745 1397 NspI r_CATG'y Cuts at: 0 225 1776 2142 Size: 225 1551 366 Fragments arranged by size: 1551 366 225 PasI CC'CwG_GG Cuts at: 0 795 1784 2142 Size: 795 989 358 Fragments arranged by size: 989 795 358 PciI A'CATG_T Cuts at: 0 1772 2142 Size: 1772 370 PflMI CCAn_nnn'nTGG Cuts at: 0 625 2142 Size: 625 1517 PpuMI rG'GwC_Cy Cuts at: 0 119 1333 1451 1811 2142 Size: 119 1214 118 360 331 Fragments arranged by size: 1214 360 331 119 118 PstI C_TGCA'G Cuts at: 0 1007 1231 1981 2142 Size: 1007 224 750 161 Fragments arranged by size: 1007 750 224 161 PvuII CAG'CTG Cuts at: 0 90 549 811 2127 2142 Size: 90 459 262 1316 15 Fragments arranged by size: 1316 459 262 90 15 SacI G_AGCT'C Cuts at: 0 29 993 2142 Size: 29 964 1149 Fragments arranged by size: 1149 964 29 SanDI GG'GwC_CC Cuts at: 0 1811 2142 Size: 1811 331 SapI GCTCTTCn'nnn_ Cuts at: 0 34 178 2142 Size: 34 144 1964 Fragments arranged by size: 1964 144 34 SbfI CC_TGCA'GG Cuts at: 0 1981 2142 Size: 1981 161 SfcI C'TryA_G Cuts at: 0 655 731 1003 1227 1977 2142 Size: 655 76 272 224 750 165 Fragments arranged by size: 750 655 272 224 165 76 SfoI GGC'GCC Cuts at: 0 861 2018 2142 Size: 861 1157 124 Fragments arranged by size: 1157 861 124 SmaI CCC'GGG Cuts at: 0 686 2142 Size: 686 1456 SmlI C'TyrA_G Cuts at: 0 872 1414 2142 Size: 872 542 728 Fragments arranged by size: 872 728 542 StuI AGG'CCT Cuts at: 0 1277 2142 Size: 1277 865 StyI C'CwwG_G Cuts at: 0 1112 1376 1917 2142 Size: 1112 264 541 225 Fragments arranged by size: 1112 541 264 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 324 331 622 1830 1852 2074 2142 Size: 324 7 291 1208 22 222 68 Fragments arranged by size: 1208 324 291 222 68 22 7 TatI w'GTAC_w Cuts at: 0 833 2142 Size: 833 1309 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2089 2121 2142 Size: 2089 32 21 Fragments arranged by size: 2089 32 21 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 792 2142 Size: 792 1350 XhoI C'TCGA_G Cuts at: 0 872 2142 Size: 872 1270 XmaI C'CCGG_G Cuts at: 0 684 2142 Size: 684 1458 Enzymes that do cut: AarI AccI AclI AcuI AflIII AleI AlfI AloI AlwNI ApoI AvaI BanI BanII BbeI BbsI BcgI BciVI BclI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI MmeI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI PpuMI PstI PvuII SacI SanDI SapI SbfI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AfeI AflII AgeI AhdI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BfrBI BglI BlpI BmgBI BmtI BplI BsaAI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BssHII BstBI BtgZI ClaI CspCI DraI DraIII EagI EcoRV FseI FspI FspAI KpnI MfeI MluI MscI NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmnI ZraI