################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HapII HincII HindII HpaI HpaII Ksp632I KspI MamI MflI MroI MspI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TfiI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 419 GGTACC G^GTACC AccII 247 CGCG CG^CG AciI 972 CCGC CCGC(-3/-1) AcsI 347 AAATTC [GA]^AATT[CT] AflIII 246 ACGCGT A^C[GA][CT]GT AlwI 334 GATCC GGATC(4/5) ApoI 347 AAATTC [GA]^AATT[CT] Asp718I 419 GGTACC G^GTACC BanII 317 GGGCTC G[GA]GC[CT]^C BbeI 789 GGCGCC GGCGC^C BfaI 424 CTAG C^TAG BglI 511 GCCATTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 94 CTGGAG CTGGAG(16/14) BsaAI 535 TACGTG [CT]AC^GT[GA] BseRI 462 GAGGAG GAGGAG(10/8) BsgI 1022 CTGCAC GTGCAG(16/14) BsmFI 949 GGGAC GGGAC(10/14) Bsp1407I 377 TGTACA T^GTACA BsrDI 114 GCAATG GCAATG(2/0) BsrGI 377 TGTACA T^GTACA BsrI 93 ACTGG ACTGG(1/-1) BstF5I 63 GGATG GGATG(2/0) BstUI 247 CGCG CG^CG CfoI 790 GCGC GCG^C Cfr13I 372 GGGCC G^G[ACGT]CC DraI 639 TTTAAA TTT^AAA FokI 63 GGATG GGATG(9/13) HaeII 789 GGCGCC [GA]GCGC^[CT] HgaI 130 GACGC GACGC(5/10) HhaI 790 GCGC GCG^C HinP1I 790 GCGC G^CGC HindIII 748 AAGCTT A^AGCTT HinfI 239 GACTC G^A[ACGT]TC HphI 88 GGTGA GGTGA(8/7) KasI 789 GGCGCC G^GCGCC KpnI 419 GGTACC GGTAC^C MaeI 424 CTAG C^TAG MaeII 536 ACGT A^CGT MfeI 396 CAATTG C^AATTG MluI 246 ACGCGT A^CGCGT MslI 1059 CACCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 396 CAATTG C^AATTG MvnI 247 CGCG CG^CG NarI 789 GGCGCC GG^CGCC PleI 239 GACTC GAGTC(4/5) Sau96I 372 GGGCC G^G[ACGT]CC SfoI 789 GGCGCC GGC^GCC SspBI 377 TGTACA T^GTACA SspI 799 AATATT AAT^ATT TaqI 1036 TCGA T^CGA TthHB8I 1036 TCGA T^CGA XcmI 41 CCAATTACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 130 GACGCC G[GA]^CG[CT]C 789 GGCGCC BalI 38 TGGCCA TGG^CCA 518 TGGCCA BbsI 559 GAAGAC GAAGAC(2/6) 772 GTCTTC BbvI 857 GCAGC GCAGC(8/12) 914 GCTGC BmyI 317 GGGCTC G[AGT]GC[ACT]^C 469 GTGCCC BpuAI 559 GAAGAC GAAGAC(2/6) 772 GTCTTC BsaHI 130 GACGCC G[GA]^CG[CT]C 789 GGCGCC BsiYI 458 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 495 CCTGGCATGGG BslI 458 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 495 CCTGGCATGGG BsmAI 385 GAGAC GTCTC(1/5) 613 GAGAC Bsp1286I 317 GGGCTC G[AGT]GC[ACT]^C 469 GTGCCC Cac8I 51 GCTGGC GC[ACGT]^[ACGT]GC 603 GCCAGC EaeI 38 TGGCCA [CT]^GGCC[GA] 518 TGGCCA Hin1I 130 GACGCC G[GA]^CG[CT]C 789 GGCGCC MluNI 38 TGGCCA TGG^CCA 518 TGGCCA MscI 38 TGGCCA TGG^CCA 518 TGGCCA MseI 19 TTAA T^TAA 640 TTAA PvuII 151 CAGCTG CAG^CTG 605 CAGCTG SfaNI 479 GCATC GCATC(5/9) 917 GCATC Tru9I 19 TTAA T^TAA 640 TTAA TseI 857 GCAGC G^C[AT]GC 914 GCTGC Tsp45I 119 GTGAC ^GT[GC]AC 845 GTGAC TspRI 381 CAGTG CA[GC]TG[ACGT][ACGT]^ 910 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AfaI AluI BanI BsaJI BssKI BstNI DdeI DpnII EcoRII Fnu4HI HaeIII ItaI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI Tsp509I