(Circular) (Six-base) MAPSORT of: pFN21AB8967.seq Check: 2716 from: 1 to: 5773 pFN21AB8967 5773 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3583 278 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3116 1937 Size: 1179 4594 Acc65I G'GTAC_C Cuts at: 2424 3094 2424 Size: 670 5103 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 3836 5220 5488 1632 Size: 425 1779 1384 268 1917 Fragments arranged by size: 1917 1779 1384 425 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5576 AflIII A'CryG_T Cuts at: 1203 2251 4672 5649 1203 Size: 1048 2421 977 1327 Fragments arranged by size: 2421 1327 1048 977 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 4502 4502 Size: 5773 AleI CACnn'nnGTG Cuts at: 1166 3068 1166 Size: 1902 3871 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5773 AlwNI CAG_nnn'CTG Cuts at: 3516 5088 3516 Size: 1572 4201 ApaLI G'TGCA_C Cuts at: 3830 4986 3830 Size: 1156 4617 ApoI r'AATT_y Cuts at: 1493 2352 3082 3308 3460 5569 1493 Size: 859 730 226 152 2109 1697 Fragments arranged by size: 2109 1697 859 730 226 152 AseI AT'TA_AT Cuts at: 160 1032 4327 160 Size: 872 3295 1606 Fragments arranged by size: 3295 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 3098 1870 Size: 73 1155 4545 Fragments arranged by size: 4545 1155 73 BamHI G'GATC_C Cuts at: 3103 3470 3103 Size: 367 5406 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2161 2424 2473 2794 Size: 325 217 627 272 102 263 49 321 Cuts at: 2794 3094 4549 618 Size: 300 1455 1842 Fragments arranged by size: 1842 1455 627 325 321 300 272 263 217 102 49 BanII G_rGCy'C Cuts at: 1391 2326 3092 1391 Size: 935 766 4072 Fragments arranged by size: 4072 935 766 BbeI G_GCGC'C Cuts at: 2798 2798 Size: 5773 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2571 2770 928 Size: 971 672 199 3931 Fragments arranged by size: 3931 971 672 199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3964 3998 1903 Size: 34 2027 34 3678 Fragments arranged by size: 3678 2027 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 1548 Size: 5773 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2522 4384 136 Size: 107 122 71 631 605 850 1862 1525 Fragments arranged by size: 1862 1525 850 631 605 122 107 71 BglII A'GATC_T Cuts at: 5768 5768 Size: 5773 BlpI GC'TnA_GC Cuts at: 3190 3190 Size: 5773 Bme1580I G_kGCm'C Cuts at: 1790 2478 3834 4990 1790 Size: 688 1356 1156 2573 Fragments arranged by size: 2573 1356 1156 688 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3504 4462 442 Size: 416 53 83 399 24 2087 958 1753 Fragments arranged by size: 2087 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 4433 1239 Size: 881 2313 2579 Fragments arranged by size: 2579 2313 881 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3898 4763 5061 3898 Size: 865 298 4610 Fragments arranged by size: 4610 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4436 882 Size: 617 2937 2219 Fragments arranged by size: 2937 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2542 3564 493 Size: 711 1338 1022 2702 Fragments arranged by size: 2702 1338 1022 711 BsaBI GATnn'nnATC Cuts at: 5767 5767 Size: 5773 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 2795 3580 3962 Size: 53 83 186 936 603 659 785 382 Cuts at: 3962 4597 275 Size: 635 1451 Fragments arranged by size: 1451 936 785 659 635 603 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4206 4604 4878 5025 5436 4206 Size: 398 274 147 411 4543 Fragments arranged by size: 4543 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2723 2753 1178 Size: 30 165 30 1320 30 4198 Fragments arranged by size: 4198 1320 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2482 1186 Size: 1296 4477 BseYI C'CCAG_C Cuts at: 2154 2330 4976 5472 2154 Size: 176 2646 496 2455 Fragments arranged by size: 2646 2455 496 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3012 1163 Size: 573 1276 3924 Fragments arranged by size: 3924 1276 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3223 3984 5012 1540 Size: 275 186 1222 761 1028 2301 Fragments arranged by size: 2301 1222 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3834 3919 4990 3834 Size: 85 1071 4617 Fragments arranged by size: 4617 1071 85 BsmI GAATG_Cn' Cuts at: 3284 3377 3284 Size: 93 5680 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2326 2478 3092 3834 3919 4990 1391 Size: 399 536 152 614 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 614 536 399 152 85 BspEI T'CCGG_A Cuts at: 5436 5436 Size: 5773 BspHI T'CATG_A Cuts at: 3662 5392 3662 Size: 1730 4043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3128 844 Size: 2284 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3660 1394 Size: 429 1837 3507 Fragments arranged by size: 3507 1837 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2126 4268 4442 65 Size: 1183 878 2142 174 1396 Fragments arranged by size: 2142 1396 1183 878 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4417 4604 1700 Size: 110 2607 187 2869 Fragments arranged by size: 2869 2607 187 110 BsrGI T'GTAC_A Cuts at: 96 2382 96 Size: 2286 3487 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5773 BssSI C'ACGA_G Cuts at: 3833 4845 3833 Size: 1012 4761 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5773 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5773 BstXI CCAn_nnnn'nTGG Cuts at: 3070 3070 Size: 5773 BstYI r'GATC_y Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768 Size: 1131 367 385 17 1452 86 12 346 Cuts at: 5768 1972 Size: 1977 Fragments arranged by size: 1977 1452 1131 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2121 2887 487 Size: 683 56 895 766 3373 Fragments arranged by size: 3373 895 766 683 56 BtsI GCAGTG_nn' Cuts at: 2914 3289 4083 4103 2914 Size: 375 794 20 4584 Fragments arranged by size: 4584 794 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5738 DraI TTT'AAA Cuts at: 2646 3077 2646 Size: 431 5342 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4780 809 Size: 507 3464 1802 Fragments arranged by size: 3464 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2523 3220 Size: 54 1425 50 275 18 213 480 697 Cuts at: 3220 4109 5738 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 697 480 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3220 1537 Size: 275 1408 4090 Fragments arranged by size: 4090 1408 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3703 1353 Size: 2350 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4360 4734 4880 387 Size: 1064 138 2771 374 146 1280 Fragments arranged by size: 2771 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2120 3836 4433 5220 5488 1239 Size: 393 425 63 1716 597 787 268 1524 Fragments arranged by size: 1716 1524 787 597 425 393 268 63 EcoRI G'AATT_C Cuts at: 3082 3460 3082 Size: 378 5395 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2467 2467 Size: 5773 FspI TGC'GCA Cuts at: 4279 4279 Size: 5773 HaeII r_GCGC'y Cuts at: 1678 2798 4920 1678 Size: 1120 2122 2531 Fragments arranged by size: 2531 2122 1120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4414 4446 4488 4520 1373 Size: 32 135 32 2842 32 42 32 2626 Fragments arranged by size: 2842 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3117 3363 669 Size: 988 281 1179 246 3079 Fragments arranged by size: 3079 1179 988 281 246 HindIII A'AGCT_T Cuts at: 748 2753 748 Size: 2005 3768 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KasI G'GCGC_C Cuts at: 2794 2794 Size: 5773 KpnI G_GTAC'C Cuts at: 2428 3098 2428 Size: 670 5103 MfeI C'AATT_G Cuts at: 2401 3372 2401 Size: 971 4802 MluI A'CGCG_T Cuts at: 2251 5649 2251 Size: 3398 2375 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4887 5071 1305 Size: 3582 184 2007 Fragments arranged by size: 3582 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 2525 10 Size: 54 1425 343 213 480 3258 Fragments arranged by size: 3258 1425 480 343 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 3068 3731 4090 4249 518 Size: 648 1902 663 359 159 2042 Fragments arranged by size: 2042 1902 663 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2612 2979 3189 3866 5014 5259 1606 Size: 552 454 367 210 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 552 454 367 245 210 NarI GG'CG_CC Cuts at: 2795 2795 Size: 5773 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5221 NdeI CA'TA_TG Cuts at: 387 387 Size: 5773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5773 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 3131 4676 1132 Size: 97 1902 1545 2229 Fragments arranged by size: 2229 1902 1545 97 PasI CC'CwG_GG Cuts at: 1348 3015 1348 Size: 1667 4106 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5773 PfoI T'CCnGG_A Cuts at: 2199 2199 Size: 5773 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5773 PsiI TTA'TAA Cuts at: 2550 3343 2550 Size: 793 4980 PstI C_TGCA'G Cuts at: 830 3125 830 Size: 2295 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 PvuII CAG'CTG Cuts at: 2158 2612 2158 Size: 454 5319 SalI G'TCGA_C Cuts at: 1936 3115 1936 Size: 1179 4594 SbfI CC_TGCA'GG Cuts at: 3125 3125 Size: 5773 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SfcI C'TryA_G Cuts at: 826 1045 3121 4256 4937 5128 826 Size: 219 2076 1135 681 191 1471 Fragments arranged by size: 2076 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2796 2796 Size: 5773 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 3100 1872 Size: 1228 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3877 4778 5040 820 Size: 197 926 1934 901 262 1553 Fragments arranged by size: 1934 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 3131 1132 Size: 1999 3774 SspI AAT'ATT Cuts at: 5 52 2806 3697 5 Size: 47 2754 891 2081 Fragments arranged by size: 2754 2081 891 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5773 StyI C'CwwG_G Cuts at: 513 1065 3212 513 Size: 552 2147 3074 Fragments arranged by size: 3074 2147 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2927 3800 3817 3970 4155 4600 325 Size: 1255 1347 873 17 153 185 445 1498 Fragments arranged by size: 1498 1347 1255 873 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2367 2382 4019 Size: 274 80 33 51 1443 390 15 1637 Cuts at: 4019 5731 96 Size: 1712 138 Fragments arranged by size: 1712 1637 1443 390 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5733 5765 5733 Size: 32 5741 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5773 XbaI T'CTAG_A Cuts at: 3109 3109 Size: 5773 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 2053 Size: 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 3098 1870 Size: 1228 4545 XmnI GAAnn'nnTTC Cuts at: 3902 3902 Size: 5773 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3581 276 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI