################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AspI AviII BamHI BbeI BbvCI BcgI BfrI BglI BsaHI BsaI BseAI BsiEI BsiWI BspDI BspEI BsrFI BssHII Bst1107I BstZ17I CfoI Cfr10I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KspI MluI MroI NaeI NarI NcoI NgoMIV NheI NotI PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### Acc65I 1958 GGTACC G^GTACC AflIII 2126 ACATGT A^C[GA][CT]GT AhdI 601 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2602 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1958 GGTACC G^GTACC AspEI 601 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1445 CCCGAG C^[CT]CG[GA]G AvrII 370 CCTAGG C^CTAGG BalI 1502 TGGCCA TGG^CCA BbrPI 1594 CACGTG CAC^GTG BciVI 2748 GTATCC GTATCC(6/5) BclI 3677 TGATCA T^GATCA BcnI 3573 CCCGG CC^[GC]GG BlnI 370 CCTAGG C^CTAGG BlpI 1624 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1624 GCTGAGC GC^T[ACGT]AGC BsaAI 1594 CACGTG [CT]AC^GT[GA] BsaBI 2328 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3367 TCCGGT [AT]^CCGG[AT] BsmBI 142 CGTCTC CGTCTC(1/5) BsmFI 2070 GGGAC GGGAC(10/14) BsoBI 1445 CCCGAG C^[CT]CG[GA]G Bsp1407I 2330 TGTACA T^GTACA BspHI 183 TCATGA T^CATGA BspLU11I 2126 ACATGT A^CATGT BsrBI 3742 GAGCGG CCGCTC(-3/-3) BsrDI 2384 GCAATG GCAATG(2/0) BsrGI 2330 TGTACA T^GTACA BssSI 2884 CTCGTG CACGAG(-5/-1) BstBI 3198 TTCGAA TT^CGAA BstEII 1489 GGTCACC G^GT[ACGT]ACC BstPI 1489 GGTCACC G^GT[ACGT]ACC CelII 1624 GCTGAGC GC^T[ACGT]AGC DsaI 2777 CCACGG C^C[GA][CT]GG EaeI 1502 TGGCCA [CT]^GGCC[GA] Eam1105I 601 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1489 GGTCACC G^GT[ACGT]ACC EcoT22I 2238 ATGCAT ATGCA^T FauI 3732 GCGGG CCCGC(4/6) FbaI 3677 TGATCA T^GATCA HgaI 875 GCGTC GACGC(5/10) KpnI 1958 GGTACC GGTAC^C MamI 2328 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1502 TGGCCA TGG^CCA MscI 1502 TGGCCA TGG^CCA NciI 3573 CCCGG CC^[GC]GG NdeI 3017 CATATG CA^TATG NruI 3038 TCGCGA TCG^CGA NsiI 2238 ATGCAT ATGCA^T NspV 3198 TTCGAA TT^CGAA PmaCI 1594 CACGTG CAC^GTG PmlI 1594 CACGTG CAC^GTG PshAI 45 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 183 TCATGA T^CATGA SacI 1627 GAGCTC GAGCT^C SfuI 3198 TTCGAA TT^CGAA SphI 3350 GCATGC GCATG^C SspBI 2330 TGTACA T^GTACA StuI 118 AGGCCT AGG^CCT XcmI 3266 CCACACTGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2602 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 959 GTAGAC GT^[AC][GT]AC 2650 GTAGAC AccII 2290 CGCG CG^CG 3039 CGCG AlwNI 1207 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 2434 CAGGAGCTG AocI 1036 CCTAAGG CC^T[ACGT]AGG 3421 CCTCAGG AseI 760 ATTAAT AT^TAAT 1677 ATTAAT AsnI 760 ATTAAT AT^TAAT 1677 ATTAAT BanI 1832 GGTGCC G^G[CT][GA]CC 1958 GGTACC BanII 1627 GAGCTC G[GA]GC[CT]^C 3734 GGGCTC BbsI 1161 GAAGAC GAAGAC(2/6) 3433 GAAGAC BpuAI 1161 GAAGAC GAAGAC(2/6) 3433 GAAGAC BsgI 231 CTGCAC GTGCAG(16/14) 2431 GTGCAG BspMI 3248 ACCTGC ACCTGC(4/8) 3437 ACCTGC BstUI 2290 CGCG CG^CG 3039 CGCG Bsu36I 1036 CCTAAGG CC^T[ACGT]AGG 3421 CCTCAGG DraIII 2425 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG 3267 CACACTGTG DrdI 1193 GACACCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3227 GACTTGTCAGTC Eco81I 1036 CCTAAGG CC^T[ACGT]AGG 3421 CCTCAGG HapII 3368 CCGG C^CGG 3574 CCGG HpaII 3368 CCGG C^CGG 3574 CCGG MaeII 1595 ACGT A^CGT 2490 ACGT MspI 3368 CCGG C^CGG 3574 CCGG MvnI 2290 CGCG CG^CG 3039 CGCG PshBI 760 ATTAAT AT^TAAT 1677 ATTAAT SapI 1428 GAAGAGC GCTCTTC(1/4) 1849 GAAGAGC SspI 169 AATATT AAT^ATT 1323 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbvI BfaI BglII BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII