(Circular) (Six-base) MAPSORT of: pFN21AE3396.seq Check: 1031 from: 1 to: 8530 pFN21AE3396 8530 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6340 278 Size: 53 83 186 936 4804 2468 Fragments arranged by size: 4804 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2965 4656 5873 1937 Size: 1028 1691 1217 4594 Fragments arranged by size: 4594 1691 1217 1028 Acc65I G'GTAC_C Cuts at: 3963 5851 3963 Size: 1888 6642 AclI AA'CG_TT Cuts at: 1563 6657 7030 1563 Size: 5094 373 3063 Fragments arranged by size: 5094 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2429 2537 2739 3682 4550 6593 7977 8245 Size: 797 108 202 943 868 2043 1384 268 Cuts at: 8245 1632 Size: 1917 Fragments arranged by size: 2043 1917 1384 943 868 797 268 202 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8333 AflIII A'CryG_T Cuts at: 1203 4131 7429 8406 1203 Size: 2928 3298 977 1327 Fragments arranged by size: 3298 2928 1327 977 AgeI A'CCGG_T Cuts at: 7361 7361 Size: 8530 AhdI GACnn_n'nnGTC Cuts at: 2611 7259 2611 Size: 4648 3882 AleI CACnn'nnGTG Cuts at: 1166 2985 3119 1166 Size: 1819 134 6577 Fragments arranged by size: 6577 1819 134 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5750 1184 Size: 4566 3964 AlwNI CAG_nnn'CTG Cuts at: 3217 4444 6273 7845 3217 Size: 1227 1829 1572 3902 Fragments arranged by size: 3902 1829 1572 1227 ApaLI G'TGCA_C Cuts at: 6587 7743 6587 Size: 1156 7374 ApoI r'AATT_y Cuts at: 1493 2303 2449 3314 4709 5607 5839 6065 6217 Size: 810 146 865 1395 898 232 226 152 Cuts at: 6217 8326 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1395 898 865 810 232 226 152 146 AseI AT'TA_AT Cuts at: 160 1032 2766 3683 7084 160 Size: 872 1734 917 3401 1606 Fragments arranged by size: 3401 1734 1606 917 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8530 AvaI C'yCGr_G Cuts at: 1870 1943 3450 5855 1870 Size: 73 1507 2405 4545 Fragments arranged by size: 4545 2405 1507 73 AvrII C'CTAG_G Cuts at: 2375 2375 Size: 8530 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3993 4026 3993 Size: 33 8497 BamHI G'GATC_C Cuts at: 5860 6227 5860 Size: 367 8163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3837 3963 5851 7306 618 Size: 325 217 627 2050 126 1888 1455 1842 Fragments arranged by size: 2050 1888 1842 1455 627 325 217 126 BanII G_rGCy'C Cuts at: 1391 3636 5743 5849 1391 Size: 2245 2107 106 4072 Fragments arranged by size: 4072 2245 2107 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3173 5445 928 Size: 971 1274 2272 4013 Fragments arranged by size: 4013 2272 1274 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6721 6755 1903 Size: 34 4784 34 3678 Fragments arranged by size: 4784 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4764 6422 7632 4764 Size: 1658 1210 5662 Fragments arranged by size: 5662 1658 1210 BclI T'GATC_A Cuts at: 1548 5682 1548 Size: 4134 4396 BfrBI ATG'CAT Cuts at: 4245 4245 Size: 8530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7141 136 Size: 107 122 71 631 605 5469 1525 Fragments arranged by size: 5469 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2694 2887 3256 8525 2694 Size: 193 369 5269 2699 Fragments arranged by size: 5269 2699 369 193 BlpI GC'TnA_GC Cuts at: 3630 5947 3630 Size: 2317 6213 Bme1580I G_kGCm'C Cuts at: 1790 2982 5829 6591 7747 1790 Size: 1192 2847 762 1156 2573 Fragments arranged by size: 2847 2573 1192 1156 762 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8530 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2149 3583 4975 Size: 416 53 83 399 24 732 1434 1392 Cuts at: 4975 5006 6261 7219 442 Size: 31 1255 958 1753 Fragments arranged by size: 1753 1434 1392 1255 958 732 416 399 83 53 31 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2584 2880 3620 4350 5765 7190 1239 Size: 1345 296 740 730 1415 1425 2579 Fragments arranged by size: 2579 1425 1415 1345 740 730 296 Bpu10I CC'TnA_GC Cuts at: 3918 5413 3918 Size: 1495 7035 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2253 3074 4578 5699 6655 7520 7818 2253 Size: 821 1504 1121 956 865 298 2965 Fragments arranged by size: 2965 1504 1121 956 865 821 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7193 882 Size: 617 5694 2219 Fragments arranged by size: 5694 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3601 6321 493 Size: 711 2397 2720 2702 Fragments arranged by size: 2720 2702 2397 711 BsaBI GATnn'nnATC Cuts at: 4337 8524 4337 Size: 4187 4343 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6337 6719 7354 275 Size: 53 83 186 936 4804 382 635 1451 Fragments arranged by size: 4804 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5372 6963 7361 7635 7782 8193 5372 Size: 1591 398 274 147 411 5709 Fragments arranged by size: 5709 1591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4763 4793 1178 Size: 30 165 30 3360 30 4915 Fragments arranged by size: 4915 3360 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2505 3165 3509 1186 Size: 856 463 660 344 6207 Fragments arranged by size: 6207 856 660 463 344 BseYI C'CCAG_C Cuts at: 3798 7733 8229 3798 Size: 3935 496 4099 Fragments arranged by size: 4099 3935 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2221 4457 1163 Size: 573 485 2236 5236 Fragments arranged by size: 5236 2236 573 485 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5980 6741 7769 1540 Size: 275 186 3979 761 1028 2301 Fragments arranged by size: 3979 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3413 3636 4992 6591 6676 7747 3413 Size: 223 1356 1599 85 1071 4196 Fragments arranged by size: 4196 1599 1356 1071 223 85 BsmI GAATG_Cn' Cuts at: 2352 2833 5139 5725 6041 6134 2352 Size: 481 2306 586 316 93 4748 Fragments arranged by size: 4748 2306 586 481 316 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2153 2153 Size: 8530 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2982 3413 3636 4992 5743 5829 5849 Size: 399 1192 431 223 1356 751 86 20 Cuts at: 5849 6591 6676 7747 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1356 1192 1071 751 742 431 399 223 86 85 20 BspEI T'CCGG_A Cuts at: 8193 8193 Size: 8530 BspHI T'CATG_A Cuts at: 2188 6419 8149 2188 Size: 4231 1730 2569 Fragments arranged by size: 4231 2569 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5262 5451 5885 844 Size: 4418 189 434 3489 Fragments arranged by size: 4418 3489 434 189 BsrBI CCG'CTC Cuts at: 1394 1823 5749 6417 1394 Size: 429 3926 668 3507 Fragments arranged by size: 3926 3507 668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4396 7025 7199 65 Size: 1183 3148 2629 174 1396 Fragments arranged by size: 3148 2629 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7174 7361 1700 Size: 110 5364 187 2869 Fragments arranged by size: 5364 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4335 96 Size: 4239 4291 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8530 BssSI C'ACGA_G Cuts at: 4889 6590 7602 4889 Size: 1701 1012 5817 Fragments arranged by size: 5817 1701 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2424 4444 5356 794 Size: 1630 2020 912 3968 Fragments arranged by size: 3968 2020 1630 912 BstBI TT'CG_AA Cuts at: 5204 7334 5204 Size: 2130 6400 BstEII G'GTnAC_C Cuts at: 1184 3494 1184 Size: 2310 6220 BstXI CCAn_nnnn'nTGG Cuts at: 4411 4904 5408 5822 4411 Size: 493 504 414 7119 Fragments arranged by size: 7119 504 493 414 BstYI r'GATC_y Cuts at: 1972 2546 2694 2707 2794 2887 3256 4414 5509 Size: 574 148 13 87 93 369 1158 1095 Cuts at: 5509 5860 6227 6612 6629 8081 8167 8179 8525 Size: 351 367 385 17 1452 86 12 346 Cuts at: 8525 1972 Size: 1977 Fragments arranged by size: 1977 1452 1158 1095 574 385 369 367 351 346 148 93 87 86 17 13 12 Bsu36I CC'TnA_GG Cuts at: 3042 5427 3042 Size: 2385 6145 BtgI C'CryG_G Cuts at: 513 1065 2004 4782 513 Size: 552 939 2778 4261 Fragments arranged by size: 4261 2778 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5060 487 Size: 683 56 3834 3957 Fragments arranged by size: 3957 3834 683 56 BtsI GCAGTG_nn' Cuts at: 3114 3126 3981 4146 5321 5492 6046 6840 6860 Size: 12 855 165 1175 171 554 794 20 Cuts at: 6860 3114 Size: 4784 Fragments arranged by size: 4784 1175 855 794 554 171 165 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4771 4806 5061 5096 529 Size: 35 4207 35 255 35 3963 Fragments arranged by size: 4207 3963 255 35 35 35 DraI TTT'AAA Cuts at: 5834 5834 Size: 8530 DraIII CAC_nnn'GTG Cuts at: 4435 5277 4435 Size: 842 7688 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3204 5238 7537 809 Size: 507 1888 2034 2299 1802 Fragments arranged by size: 2299 2034 1888 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3507 5977 6866 Size: 54 1425 50 275 18 1677 2470 889 Cuts at: 6866 8495 8 Size: 1629 43 Fragments arranged by size: 2470 1677 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5977 1537 Size: 275 4165 4090 Fragments arranged by size: 4165 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2032 2451 3428 3849 4602 6460 1353 Size: 679 419 977 421 753 1858 3423 Fragments arranged by size: 3423 1858 977 753 679 421 419 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7117 7491 7637 387 Size: 1064 138 5528 374 146 1280 Fragments arranged by size: 5528 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3634 3634 Size: 8530 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2429 2537 2584 2739 2880 3620 3682 Size: 393 797 108 47 155 141 740 62 Cuts at: 3682 4350 4550 5765 6593 7190 7977 8245 1239 Size: 668 200 1215 828 597 787 268 1524 Fragments arranged by size: 1524 1215 828 797 787 740 668 597 393 268 200 155 141 108 62 47 EcoO109I rG'GnC_Cy Cuts at: 2396 3214 3444 3950 5422 5638 2396 Size: 818 230 506 1472 216 5288 Fragments arranged by size: 5288 1472 818 506 230 216 EcoRI G'AATT_C Cuts at: 5839 6217 5839 Size: 378 8152 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2404 2829 4631 2404 Size: 425 1802 6303 Fragments arranged by size: 6303 1802 425 FspI TGC'GCA Cuts at: 7036 7036 Size: 8530 HaeII r_GCGC'y Cuts at: 1678 7677 1678 Size: 5999 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2597 2629 2858 2890 3160 Size: 32 135 32 1025 32 229 32 270 Cuts at: 3160 3192 3206 3225 3238 3257 4220 4252 4763 Size: 32 14 19 13 19 963 32 511 Cuts at: 4763 4795 5302 5334 5516 5548 5573 5605 7171 Size: 32 507 32 182 32 25 32 1566 Cuts at: 7171 7203 7245 7277 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1566 1025 963 511 507 270 229 182 135 42 32 32 32 32 32 32 32 32 32 32 32 32 25 19 19 14 13 HincII GTy'rAC Cuts at: 669 1657 1938 2993 4137 4792 5874 6120 669 Size: 988 281 1055 1144 655 1082 246 3079 Fragments arranged by size: 3079 1144 1082 1055 988 655 281 246 HindIII A'AGCT_T Cuts at: 748 2748 2850 5654 748 Size: 2000 102 2804 3624 Fragments arranged by size: 3624 2804 2000 102 HpaI GTT'AAC Cuts at: 6120 6120 Size: 8530 KpnI G_GTAC'C Cuts at: 3967 5855 3967 Size: 1888 6642 MfeI C'AATT_G Cuts at: 3062 3539 3663 6129 3062 Size: 477 124 2466 5463 Fragments arranged by size: 5463 2466 477 124 MluI A'CGCG_T Cuts at: 8406 8406 Size: 8530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3050 7644 7828 1305 Size: 1745 4594 184 2007 Fragments arranged by size: 4594 2007 1745 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3509 10 Size: 54 1425 343 1677 5031 Fragments arranged by size: 5031 1677 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2985 3119 4151 5536 6488 6847 7006 Size: 648 1819 134 1032 1385 952 359 159 Cuts at: 7006 518 Size: 2042 Fragments arranged by size: 2042 1819 1385 1032 952 648 359 159 134 MspA1I CmG'CkG Cuts at: 1606 3629 4528 5560 5946 6623 7771 8016 1606 Size: 2023 899 1032 386 677 1148 245 2120 Fragments arranged by size: 2120 2023 1148 1032 899 677 386 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7039 Fragments arranged by size: 7039 939 552 NdeI CA'TA_TG Cuts at: 387 5023 387 Size: 4636 3894 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8530 NotI GC'GGCC_GC Cuts at: 5977 5977 Size: 8530 NruI TCG'CGA Cuts at: 5045 5045 Size: 8530 NsiI A_TGCA'T Cuts at: 4247 4247 Size: 8530 NspI r_CATG'y Cuts at: 1132 1229 4135 4245 5359 5888 7433 1132 Size: 97 2906 110 1114 529 1545 2229 Fragments arranged by size: 2906 2229 1545 1114 529 110 97 PasI CC'CwG_GG Cuts at: 1348 3925 5759 1348 Size: 2577 1834 4119 Fragments arranged by size: 4119 2577 1834 PciI A'CATG_T Cuts at: 4131 7429 4131 Size: 3298 5232 PflMI CCAn_nnn'nTGG Cuts at: 1108 3505 3737 3960 4837 1108 Size: 2397 232 223 877 4801 Fragments arranged by size: 4801 2397 877 232 223 PfoI T'CCnGG_A Cuts at: 3636 3636 Size: 8530 PmeI GTTT'AAAC Cuts at: 5834 5834 Size: 8530 PmlI CAC'GTG Cuts at: 3601 3601 Size: 8530 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6599 6631 8112 8144 6599 Size: 32 1481 32 6985 Fragments arranged by size: 6985 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3214 3950 5422 5638 3214 Size: 736 1472 216 6106 Fragments arranged by size: 6106 1472 736 216 PshAI GACnn'nnGTC Cuts at: 1613 2054 1613 Size: 441 8089 PsiI TTA'TAA Cuts at: 4290 6100 4290 Size: 1810 6720 PstI C_TGCA'G Cuts at: 830 2832 3121 5437 5882 830 Size: 2002 289 2316 445 3478 Fragments arranged by size: 3478 2316 2002 445 289 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8530 PvuII CAG'CTG Cuts at: 3629 4528 5560 3629 Size: 899 1032 6599 Fragments arranged by size: 6599 1032 899 SacI G_AGCT'C Cuts at: 3636 3636 Size: 8530 SalI G'TCGA_C Cuts at: 1936 5872 1936 Size: 3936 4594 SapI GCTCTTCn'nnn_ Cuts at: 3428 3849 3428 Size: 421 8109 SbfI CC_TGCA'GG Cuts at: 5882 5882 Size: 8530 ScaI AGT'ACT Cuts at: 6778 8490 6778 Size: 1712 6818 SfcI C'TryA_G Cuts at: 826 1045 2828 3117 4554 4653 5433 5878 7013 Size: 219 1783 289 1437 99 780 445 1135 Cuts at: 7013 7694 7885 826 Size: 681 191 1471 Fragments arranged by size: 1783 1471 1437 1135 780 681 445 289 219 191 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8530 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8530 SmaI CCC'GGG Cuts at: 1872 5857 1872 Size: 3985 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2268 3053 4593 5714 6634 7535 Size: 197 926 325 785 1540 1121 920 901 Cuts at: 7535 7797 820 Size: 262 1553 Fragments arranged by size: 1553 1540 1121 926 920 901 785 325 262 197 SnaBI TAC'GTA Cuts at: 493 6321 493 Size: 5828 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8530 SphI G_CATG'C Cuts at: 1132 5359 5888 1132 Size: 4227 529 3774 Fragments arranged by size: 4227 3774 529 SspI AAT'ATT Cuts at: 5 52 2176 3330 6454 5 Size: 47 2124 1154 3124 2081 Fragments arranged by size: 3124 2124 2081 1154 47 StuI AGG'CCT Cuts at: 1517 2125 1517 Size: 608 7922 StyI C'CwwG_G Cuts at: 513 1065 2004 2375 2937 3588 3736 3810 4476 Size: 552 939 371 562 651 148 74 666 Cuts at: 4476 4809 4818 5631 5796 5969 513 Size: 333 9 813 165 173 3074 Fragments arranged by size: 3074 939 813 666 651 562 552 371 333 173 165 148 74 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3779 4448 4518 6557 6574 6727 6912 Size: 1255 2199 669 70 2039 17 153 185 Cuts at: 6912 7357 325 Size: 445 1498 Fragments arranged by size: 2199 2039 1498 1255 669 445 185 153 70 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2105 4128 4335 Size: 274 80 33 51 1443 128 2023 207 Cuts at: 4335 6776 8488 96 Size: 2441 1712 138 Fragments arranged by size: 2441 2023 1712 1443 274 207 138 128 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8490 8522 8490 Size: 32 8498 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8530 XbaI T'CTAG_A Cuts at: 5866 5866 Size: 8530 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5278 5278 Size: 8530 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8530 XmaI C'CCGG_G Cuts at: 1870 5855 1870 Size: 3985 4545 XmnI GAAnn'nnTTC Cuts at: 4611 6659 4611 Size: 2048 6482 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6338 276 Size: 53 83 186 936 4804 2468 Fragments arranged by size: 4804 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BplI BsiWI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI