(Linear) (Six-base) MAPSORT of: KIEE3396.seq Check: 8717 from: 1 to: 3825 KIEE3396.orf With 182 enzymes: SgfI * January 29, 2014 11:45 .. AccI GT'mk_AC Cuts at: 0 960 2651 3825 Size: 960 1691 1174 Fragments arranged by size: 1691 1174 960 Acc65I G'GTAC_C Cuts at: 0 1958 3825 Size: 1958 1867 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 532 734 1677 2545 3825 Size: 424 108 202 943 868 1280 Fragments arranged by size: 1280 943 868 424 202 108 AflIII A'CryG_T Cuts at: 0 2126 3825 Size: 2126 1699 AhdI GACnn_n'nnGTC Cuts at: 0 606 3825 Size: 606 3219 AleI CACnn'nnGTG Cuts at: 0 980 1114 3825 Size: 980 134 2711 Fragments arranged by size: 2711 980 134 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3745 3825 Size: 3745 80 AlwNI CAG_nnn'CTG Cuts at: 0 1212 2439 3825 Size: 1212 1227 1386 Fragments arranged by size: 1386 1227 1212 ApoI r'AATT_y Cuts at: 0 298 444 1309 2704 3602 3825 Size: 298 146 865 1395 898 223 Fragments arranged by size: 1395 898 865 298 223 146 AseI AT'TA_AT Cuts at: 0 761 1678 3825 Size: 761 917 2147 Fragments arranged by size: 2147 917 761 AvaI C'yCGr_G Cuts at: 0 1445 3825 Size: 1445 2380 AvrII C'CTAG_G Cuts at: 0 370 3825 Size: 370 3455 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1988 2021 3825 Size: 1988 33 1804 Fragments arranged by size: 1988 1804 33 BanI G'GyrC_C Cuts at: 0 1832 1958 3825 Size: 1832 126 1867 Fragments arranged by size: 1867 1832 126 BanII G_rGCy'C Cuts at: 0 1631 3738 3825 Size: 1631 2107 87 Fragments arranged by size: 2107 1631 87 BbsI GAAGACnn'nnnn_ Cuts at: 0 1168 3440 3825 Size: 1168 2272 385 Fragments arranged by size: 2272 1168 385 BciVI GTATCCnnnnn_n' Cuts at: 0 2759 3825 Size: 2759 1066 BclI T'GATC_A Cuts at: 0 3677 3825 Size: 3677 148 BfrBI ATG'CAT Cuts at: 0 2240 3825 Size: 2240 1585 BglII A'GATC_T Cuts at: 0 689 882 1251 3825 Size: 689 193 369 2574 Fragments arranged by size: 2574 689 369 193 BlpI GC'TnA_GC Cuts at: 0 1625 3825 Size: 1625 2200 Bme1580I G_kGCm'C Cuts at: 0 977 3824 3825 Size: 977 2847 1 Fragments arranged by size: 2847 977 1 BmrI ACTGGGnnnn_n' Cuts at: 0 144 1578 2970 3001 3825 Size: 144 1434 1392 31 824 Fragments arranged by size: 1434 1392 824 144 31 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 579 875 1615 2345 3760 3825 Size: 579 296 740 730 1415 65 Fragments arranged by size: 1415 740 730 579 296 65 Bpu10I CC'TnA_GC Cuts at: 0 1913 3408 3825 Size: 1913 1495 417 Fragments arranged by size: 1913 1495 417 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 248 1069 2573 3694 3825 Size: 248 821 1504 1121 131 Fragments arranged by size: 1504 1121 821 248 131 BsaAI yAC'GTr Cuts at: 0 1596 3825 Size: 1596 2229 BsaBI GATnn'nnATC Cuts at: 0 2332 3825 Size: 2332 1493 BsaWI w'CCGG_w Cuts at: 0 3367 3825 Size: 3367 458 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2758 2788 3825 Size: 2758 30 1037 Fragments arranged by size: 2758 1037 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 500 1160 1504 3825 Size: 37 463 660 344 2321 Fragments arranged by size: 2321 660 463 344 37 BseYI C'CCAG_C Cuts at: 0 1793 3825 Size: 1793 2032 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 216 2452 3825 Size: 216 2236 1373 Fragments arranged by size: 2236 1373 216 BsiHKAI G_wGCw'C Cuts at: 0 1408 1631 2987 3825 Size: 1408 223 1356 838 Fragments arranged by size: 1408 1356 838 223 BsmI GAATG_Cn' Cuts at: 0 347 828 3134 3720 3825 Size: 347 481 2306 586 105 Fragments arranged by size: 2306 586 481 347 105 BsmBI CGTCTCn'nnnn_ Cuts at: 0 148 3825 Size: 148 3677 Bsp1286I G_dGCh'C Cuts at: 0 977 1408 1631 2987 3738 3824 3825 Size: 977 431 223 1356 751 86 1 Fragments arranged by size: 1356 977 751 431 223 86 1 BspHI T'CATG_A Cuts at: 0 183 3825 Size: 183 3642 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3257 3446 3825 Size: 3257 189 379 Fragments arranged by size: 3257 379 189 BsrBI CCG'CTC Cuts at: 0 3744 3825 Size: 3744 81 BsrDI GCAATG_nn' Cuts at: 0 2391 3825 Size: 2391 1434 BsrGI T'GTAC_A Cuts at: 0 2330 3825 Size: 2330 1495 BssSI C'ACGA_G Cuts at: 0 2884 3825 Size: 2884 941 BstAPI GCAn_nnn'nTGC Cuts at: 0 419 2439 3351 3825 Size: 419 2020 912 474 Fragments arranged by size: 2020 912 474 419 BstBI TT'CG_AA Cuts at: 0 3199 3825 Size: 3199 626 BstEII G'GTnAC_C Cuts at: 0 1489 3825 Size: 1489 2336 BstXI CCAn_nnnn'nTGG Cuts at: 0 2406 2899 3403 3817 3825 Size: 2406 493 504 414 8 Fragments arranged by size: 2406 504 493 414 8 BstYI r'GATC_y Cuts at: 0 541 689 702 789 882 1251 2409 3504 Size: 541 148 13 87 93 369 1158 1095 Cuts at: 3504 3825 Size: 321 Fragments arranged by size: 1158 1095 541 369 321 148 93 87 13 Bsu36I CC'TnA_GG Cuts at: 0 1037 3422 3825 Size: 1037 2385 403 Fragments arranged by size: 2385 1037 403 BtgI C'CryG_G Cuts at: 0 2777 3825 Size: 2777 1048 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3055 3825 Size: 3055 770 BtsI GCAGTG_nn' Cuts at: 0 1109 1121 1976 2141 3316 3487 3825 Size: 1109 12 855 165 1175 171 338 Fragments arranged by size: 1175 1109 855 338 171 165 12 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2766 2801 3056 3091 3825 Size: 2766 35 255 35 734 Fragments arranged by size: 2766 734 255 35 35 DraIII CAC_nnn'GTG Cuts at: 0 2430 3272 3825 Size: 2430 842 553 Fragments arranged by size: 2430 842 553 DrdI GACnn_nn'nnGTC Cuts at: 0 1199 3233 3825 Size: 1199 2034 592 Fragments arranged by size: 2034 1199 592 EaeI y'GGCC_r Cuts at: 0 1502 3825 Size: 1502 2323 EarI CTCTTCn'nnn_ Cuts at: 0 27 446 1423 1844 2597 3825 Size: 27 419 977 421 753 1228 Fragments arranged by size: 1228 977 753 421 419 27 EcoICRI GAG'CTC Cuts at: 0 1629 3825 Size: 1629 2196 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 532 579 734 875 1615 1677 2345 Size: 424 108 47 155 141 740 62 668 Cuts at: 2345 2545 3760 3825 Size: 200 1215 65 Fragments arranged by size: 1215 740 668 424 200 155 141 108 65 62 47 EcoO109I rG'GnC_Cy Cuts at: 0 391 1209 1439 1945 3417 3633 3825 Size: 391 818 230 506 1472 216 192 Fragments arranged by size: 1472 818 506 391 230 216 192 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 399 824 2626 3825 Size: 399 425 1802 1199 Fragments arranged by size: 1802 1199 425 399 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 592 624 853 885 1155 1187 1201 1220 Size: 592 32 229 32 270 32 14 19 Cuts at: 1220 1233 1252 2215 2247 2758 2790 3297 3329 Size: 13 19 963 32 511 32 507 32 Cuts at: 3329 3511 3543 3568 3600 3825 Size: 182 32 25 32 225 Fragments arranged by size: 963 592 511 507 270 229 225 182 32 32 32 32 32 32 32 32 25 19 19 14 13 HincII GTy'rAC Cuts at: 0 988 2132 2787 3825 Size: 988 1144 655 1038 Fragments arranged by size: 1144 1038 988 655 HindIII A'AGCT_T Cuts at: 0 743 845 3649 3825 Size: 743 102 2804 176 Fragments arranged by size: 2804 743 176 102 KpnI G_GTAC'C Cuts at: 0 1962 3825 Size: 1962 1863 MfeI C'AATT_G Cuts at: 0 1057 1534 1658 3825 Size: 1057 477 124 2167 Fragments arranged by size: 2167 1057 477 124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1045 3825 Size: 1045 2780 MscI TGG'CCA Cuts at: 0 1504 3825 Size: 1504 2321 MslI CAynn'nnrTG Cuts at: 0 980 1114 2146 3531 3825 Size: 980 134 1032 1385 294 Fragments arranged by size: 1385 1032 980 294 134 MspA1I CmG'CkG Cuts at: 0 1624 2523 3555 3825 Size: 1624 899 1032 270 Fragments arranged by size: 1624 1032 899 270 NdeI CA'TA_TG Cuts at: 0 3018 3825 Size: 3018 807 NruI TCG'CGA Cuts at: 0 3040 3825 Size: 3040 785 NsiI A_TGCA'T Cuts at: 0 2242 3825 Size: 2242 1583 NspI r_CATG'y Cuts at: 0 2130 2240 3354 3825 Size: 2130 110 1114 471 Fragments arranged by size: 2130 1114 471 110 PasI CC'CwG_GG Cuts at: 0 1920 3754 3825 Size: 1920 1834 71 Fragments arranged by size: 1920 1834 71 PciI A'CATG_T Cuts at: 0 2126 3825 Size: 2126 1699 PflMI CCAn_nnn'nTGG Cuts at: 0 1500 1732 1955 2832 3825 Size: 1500 232 223 877 993 Fragments arranged by size: 1500 993 877 232 223 PfoI T'CCnGG_A Cuts at: 0 1631 3825 Size: 1631 2194 PmlI CAC'GTG Cuts at: 0 1596 3825 Size: 1596 2229 PpuMI rG'GwC_Cy Cuts at: 0 1209 1945 3417 3633 3825 Size: 1209 736 1472 216 192 Fragments arranged by size: 1472 1209 736 216 192 PshAI GACnn'nnGTC Cuts at: 0 49 3825 Size: 49 3776 PsiI TTA'TAA Cuts at: 0 2285 3825 Size: 2285 1540 PstI C_TGCA'G Cuts at: 0 827 1116 3432 3825 Size: 827 289 2316 393 Fragments arranged by size: 2316 827 393 289 PvuII CAG'CTG Cuts at: 0 1624 2523 3555 3825 Size: 1624 899 1032 270 Fragments arranged by size: 1624 1032 899 270 SacI G_AGCT'C Cuts at: 0 1631 3825 Size: 1631 2194 SapI GCTCTTCn'nnn_ Cuts at: 0 1423 1844 3825 Size: 1423 421 1981 Fragments arranged by size: 1981 1423 421 SfcI C'TryA_G Cuts at: 0 823 1112 2549 2648 3428 3825 Size: 823 289 1437 99 780 397 Fragments arranged by size: 1437 823 780 397 289 99 SmlI C'TyrA_G Cuts at: 0 263 1048 2588 3709 3825 Size: 263 785 1540 1121 116 Fragments arranged by size: 1540 1121 785 263 116 SphI G_CATG'C Cuts at: 0 3354 3825 Size: 3354 471 SspI AAT'ATT Cuts at: 0 171 1325 3825 Size: 171 1154 2500 Fragments arranged by size: 2500 1154 171 StuI AGG'CCT Cuts at: 0 120 3825 Size: 120 3705 StyI C'CwwG_G Cuts at: 0 370 932 1583 1731 1805 2471 2804 2813 Size: 370 562 651 148 74 666 333 9 Cuts at: 2813 3626 3791 3825 Size: 813 165 34 Fragments arranged by size: 813 666 651 562 370 333 165 148 74 34 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1774 2443 2513 3825 Size: 1774 669 70 1312 Fragments arranged by size: 1774 1312 669 70 TatI w'GTAC_w Cuts at: 0 100 2123 2330 3825 Size: 100 2023 207 1495 Fragments arranged by size: 2023 1495 207 100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3273 3825 Size: 3273 552 XmnI GAAnn'nnTTC Cuts at: 0 2606 3825 Size: 2606 1219 Enzymes that do cut: AccI Acc65I AcuI AflIII AhdI AleI AlfI AlwNI ApoI AseI AvaI AvrII BaeI BanI BanII BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NdeI NruI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PsiI PstI PvuII SacI SapI SfcI SmlI SphI SspI StuI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AloI ApaI ApaLI AscI AsiSI BamHI BbeI BbvCI BcgI BglI BmgBI BmtI BplI BsaI BsaHI BsiEI BsiWI BspEI BsrFI BssHII BstZ17I ClaI DraI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI MluI NaeI NarI NcoI NgoMIV NheI NotI PacI PmeI PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI XhoI XmaI ZraI