################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AhdI Alw44I AocI Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI HphI KpnI MaeI MamI MfeI MluI MroI MseI MslI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I TspRI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 601 GTCTAC GT^[AC][GT]AC AgeI 719 ACCGGT A^CCGGT AlwI 675 GATCC GGATC(4/5) AscI 97 GGCGCGCC GG^CGCGCC BalI 665 TGGCCA TGG^CCA BglII 701 AGATCT A^GATCT BsaI 64 GAGACC GGTCTC(1/5) BsaWI 719 ACCGGT [AT]^CCGG[AT] BsmBI 294 CGTCTC CGTCTC(1/5) BsmFI 810 GTCCC GGGAC(10/14) BstF5I 45 CATCC GGATG(2/0) BstYI 701 AGATCT [GA]^GATC[CT] CpoI 897 CGGTCCG CG^G[AT]CCG DraII 160 GGGCCCT [GA]G^G[ACGT]CC[CT] EarI 583 CTCTTC CTCTTC(1/4) EcoO109I 160 GGGCCCT [GA]G^G[ACGT]CC[CT] FokI 45 CATCC GGATG(9/13) Ksp632I 583 CTCTTC CTCTTC(1/4) MaeII 1057 ACGT A^CGT MaeIII 218 GTCAC ^GT[ACGT]AC MboII 584 TCTTC GAAGA(8/7) MflI 701 AGATCT [GA]^GATC[CT] MluNI 665 TGGCCA TGG^CCA MscI 665 TGGCCA TGG^CCA PflMI 398 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 719 ACCGGT A^CCGGT PstI 766 CTGCAG CTGCA^G RsrII 897 CGGTCCG CG^G[AT]CCG SapI 582 GCTCTTC GCTCTTC(1/4) SfaNI 503 GCATC GCATC(5/9) SfcI 766 CTGCAG C^T[GA][CT]AG SmaI 972 CCCGGG CCC^GGG TaqI 59 TCGA T^CGA Tsp45I 218 GTCAC ^GT[GC]AC TthHB8I 59 TCGA T^CGA Van91I 398 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 701 AGATCT [GA]^GATC[CT] XmaCI 972 CCCGGG C^CCGGG XmaI 972 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AspHI 580 GTGCTC G[AT]GC[AT]^C 658 GAGCAC AvaI 649 CCCGAG C^[CT]CG[GA]G 972 CCCGGG AvaII 423 GGACC G^G[AT]CC 898 GGTCC BsgI 535 CTGCAC GTGCAG(16/14) 964 CTGCAC BsiHKAI 580 GTGCTC G[AT]GC[AT]^C 658 GAGCAC BsmAI 64 GAGAC GTCTC(1/5) 295 GTCTC BsoBI 649 CCCGAG C^[CT]CG[GA]G 972 CCCGGG BsrBI 557 CCGCTC CCGCTC(-3/-3) 613 GAGCGG BssHII 98 GCGCGC G^CGCGC 146 GCGCGC BstXI 446 CCAGGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 518 CCAAGTCGCTGG DdeI 49 CTGAG C^T[ACGT]AG 929 CTGAG DpnII 675 GATC ^GATC 702 GATC KspI 131 CCGCGG CCGC^GG 233 CCGCGG MboI 675 GATC ^GATC 702 GATC NaeI 481 GCCGGC GCC^GGC 909 GCCGGC NdeII 675 GATC ^GATC 702 GATC NgoMIV 481 GCCGGC G^CCGGC 909 GCCGGC PvuII 251 CAGCTG CAG^CTG 763 CAGCTG SacII 131 CCGCGG CCGC^GG 233 CCGCGG Sau3AI 675 GATC ^GATC 702 GATC SphI 191 GCATGC GCATG^C 380 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI ApaI BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiYI BslI Bsp1286I BspMI BsrFI BssKI BstAPI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DsaI EaeI EagI EclXI Eco52I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NlaIII NlaIV NotI NspI RsaI Sau96I ScrFI SfoI StyI TseI