Restriction map for
pFN21AE0789
(Circular) (Six-base) MAPSORT of: pFN21AE0789.seq Check: 7530 from: 1 to: 5767
pFN21AE0789 5767 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2607 3110 1937
Size: 670 503 4594
Fragments arranged by size:
4594 670 503
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3830 5214 5482 1632
Size: 2198 1384 268 1917
Fragments arranged by size:
2198 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 4666 5643 1203
Size: 3463 977 1327
Fragments arranged by size:
3463 1327 977
AgeI A'CCGG_T
Cuts at: 2724 4598 2724
Size: 1874 3893
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 2258 2334 2770 2933 3510 5082 2258
Size: 76 436 163 577 1572 2943
Fragments arranged by size:
2943 1572 577 436 163 76
ApaI G_GGCC'C
Cuts at: 2169 2537 2795 2169
Size: 368 258 5141
Fragments arranged by size:
5141 368 258
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AscI GG'CGCG_CC
Cuts at: 2103 2103
Size: 5767
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2654 2977 3092 1870
Size: 73 711 323 115 4545
Fragments arranged by size:
4545 711 323 115 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2122 2318 2417 2650
Size: 325 217 627 303 32 196 99 233
Cuts at: 2650 2833 2873 3038 3088 4543 618
Size: 183 40 165 50 1455 1842
Fragments arranged by size:
1842 1455 627 325 303 233 217 196
183 165 99 50 40 32
BanII G_rGCy'C
Cuts at: 1391 2018 2060 2169 2537 2795 3086 1391
Size: 627 42 109 368 258 291 4072
Fragments arranged by size:
4072 627 368 291 258 109 42
BbeI G_GCGC'C
Cuts at: 2094 2126 2322 2421 2654 2877 3042 2094
Size: 32 196 99 233 223 165 4819
Fragments arranged by size:
4819 233 223 196 165 99 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4796
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2164 2243 2871
Size: 107 122 71 631 605 492 79 628
Cuts at: 2871 2982 4378 136
Size: 111 1396 1525
Fragments arranged by size:
1525 1396 631 628 605 492 122 111
107 79 71
BglII A'GATC_T
Cuts at: 2706 5762 2706
Size: 3056 2711
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2537 2795 3828 4984 1790
Size: 379 368 258 1033 1156 2573
Fragments arranged by size:
2573 1156 1033 379 368 258
BmgBI CAC'GTC
Cuts at: 1318 3063 1318
Size: 1745 4022
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2434 2636 2960 4427 1239
Size: 1195 202 324 1467 2579
Fragments arranged by size:
2579 1467 1195 324 202
Bpu10I CC'TnA_GC
Cuts at: 2054 2934 2054
Size: 880 4887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2063 4430 882
Size: 617 564 2367 2219
Fragments arranged by size:
2367 2219 617 564
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 2091 2123 2298
Size: 53 83 186 936 497 61 32 175
Cuts at: 2298 2319 2418 2517 2651 2690 2874 2924 3030
Size: 21 99 99 134 39 184 50 106
Cuts at: 3030 3039 3574 3956 4591 275
Size: 9 535 382 635 1451
Fragments arranged by size:
1451 936 635 535 497 382 186 184
175 134 106 99 99 83 61 53
50 39 32 21 9
BsaWI w'CCGG_w
Cuts at: 2724 4200 4598 4872 5019 5430 2724
Size: 1476 398 274 147 411 3061
Fragments arranged by size:
3061 1476 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2933 2963 1178
Size: 30 165 30 1530 30 3982
Fragments arranged by size:
3982 1530 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2161 2957 1186
Size: 895 80 796 3996
Fragments arranged by size:
3996 895 796 80
BseYI C'CCAG_C
Cuts at: 2530 2954 4970 5466 2530
Size: 424 2016 496 2831
Fragments arranged by size:
2831 2016 496 424
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2525 2954 1163
Size: 573 789 429 3976
Fragments arranged by size:
3976 789 573 429
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2142 2187 2238 2274 3009 3217
Size: 275 186 141 45 51 36 735 208
Cuts at: 3217 3978 5006 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 735 275 208 186 141
51 45 36
BsiHKAI G_wGCw'C
Cuts at: 2589 2667 3828 3913 4984 2589
Size: 78 1161 85 1071 3372
Fragments arranged by size:
3372 1161 1071 85 78
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
BsmBI CGTCTCn'nnnn_
Cuts at: 2305 2305
Size: 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2060 2169 2537 2589 2667 2795
Size: 399 228 42 109 368 52 78 128
Cuts at: 2795 3086 3828 3913 4984 1391
Size: 291 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 291 228 128
109 85 78 52 42
BspEI T'CCGG_A
Cuts at: 5430 5430
Size: 5767
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2593 2689 3054 3122 844
Size: 1749 96 365 68 3489
Fragments arranged by size:
3489 1749 365 96 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2564 2620 3654 1394
Size: 429 741 56 1034 3507
Fragments arranged by size:
3507 1034 741 429 56
BsrDI GCAATG_nn'
Cuts at: 65 1248 4262 4436 65
Size: 1183 3014 174 1396
Fragments arranged by size:
3014 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2405 2486 2504 2724 2900 2914 4411
Size: 110 595 81 18 220 176 14 1497
Cuts at: 4411 4598 1700
Size: 187 2869
Fragments arranged by size:
2869 1497 595 220 187 176 110 81
18 14
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 2103 2151 1925
Size: 178 48 5541
Fragments arranged by size:
5541 178 48
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2258 2334 2770 2862 794
Size: 1464 76 436 92 3699
Fragments arranged by size:
3699 1464 436 92 76
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 2530 2458
Size: 72 5695
BstYI r'GATC_y
Cuts at: 1972 2706 3097 3464 3849 3866 5318 5404 5416
Size: 734 391 367 385 17 1452 86 12
Cuts at: 5416 5762 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 734 391 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2094 2136 2187 2214 2238 2322 513
Size: 552 1029 42 51 27 24 84 3958
Fragments arranged by size:
3958 1029 552 84 51 42 27 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2494 487
Size: 683 56 1268 3760
Fragments arranged by size:
3760 1268 683 56
BtsI GCAGTG_nn'
Cuts at: 3283 4077 4097 3283
Size: 794 20 4953
Fragments arranged by size:
4953 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2235 2271
Size: 54 1425 50 275 18 309 96 36
Cuts at: 2271 2400 2484 2670 2893 3006 3214 4103 5732
Size: 129 84 186 223 113 208 889 1629
Cuts at: 5732 8
Size: 43
Fragments arranged by size:
1629 1425 889 309 275 223 208 186
129 113 96 84 54 50 43 36
18
EagI C'GGCC_G
Cuts at: 1537 1812 2139 2235 2271 3006 3214 1537
Size: 275 327 96 36 735 208 4090
Fragments arranged by size:
4090 735 327 275 208 96 36
EarI CTCTTCn'nnn_
Cuts at: 1353 2594 3697 1353
Size: 1241 1103 3423
Fragments arranged by size:
3423 1241 1103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2796 4354 4728 4874 387
Size: 1064 138 1207 1558 374 146 1280
Fragments arranged by size:
1558 1280 1207 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2434 2636 2960 3830 4427 5214 5482
Size: 393 802 202 324 870 597 787 268
Cuts at: 5482 1239
Size: 1524
Fragments arranged by size:
1524 870 802 787 597 393 324 268
202
EcoO109I rG'GnC_Cy
Cuts at: 2166 2166
Size: 5767
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2094 2126 2322 2421 2581 2626 2654 2877
Size: 416 32 196 99 160 45 28 223
Cuts at: 2877 3042 4914 1678
Size: 165 1872 2531
Fragments arranged by size:
2531 1872 416 223 196 165 160 99
45 32 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2933 2965 4408 4440 4482
Size: 32 135 32 1361 32 1443 32 42
Cuts at: 4482 4514 1373
Size: 32 2626
Fragments arranged by size:
2626 1443 1361 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KasI G'GCGC_C
Cuts at: 2090 2122 2318 2417 2650 2873 3038 2090
Size: 32 196 99 233 223 165 4819
Fragments arranged by size:
4819 233 223 196 165 99 32
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2070 4881 5065 1305
Size: 765 2811 184 2007
Fragments arranged by size:
2811 2007 765 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2672 10
Size: 54 1425 343 840 3105
Fragments arranged by size:
3105 1425 840 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3725 4084 4243 518
Size: 648 2559 359 159 2042
Fragments arranged by size:
2559 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2138 2240 2258 2539 2770 3024 3183
Size: 482 50 102 18 281 231 254 159
Cuts at: 3183 3860 5008 5253 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 482 281 254 245 231
159 102 50 18
NaeI GCC'GGC
Cuts at: 2488 2916 2488
Size: 428 5339
NarI GG'CG_CC
Cuts at: 2091 2123 2319 2418 2651 2874 3039 2091
Size: 32 196 99 233 223 165 4819
Fragments arranged by size:
4819 233 223 196 165 99 32
NcoI C'CATG_G
Cuts at: 513 1065 2094 2214 2322 513
Size: 552 1029 120 108 3958
Fragments arranged by size:
3958 1029 552 120 108
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NgoMIV G'CCGG_C
Cuts at: 2486 2914 2486
Size: 428 5339
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 2139 2235 2271 3006 3214 2139
Size: 96 36 735 208 4692
Fragments arranged by size:
4692 735 208 96 36
NspI r_CATG'y
Cuts at: 1132 1229 2200 2290 2389 3125 4670 1132
Size: 97 971 90 99 736 1545 2229
Fragments arranged by size:
2229 1545 971 736 99 97 90
PasI CC'CwG_GG
Cuts at: 1348 2128 1348
Size: 780 4987
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2409 1108
Size: 1301 4466
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PspOMI G'GGCC_C
Cuts at: 2165 2533 2791 2165
Size: 368 258 5141
Fragments arranged by size:
5141 368 258
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2711 2743 2711
Size: 32 5735
PstI C_TGCA'G
Cuts at: 830 2775 3119 830
Size: 1945 344 3478
Fragments arranged by size:
3478 1945 344
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2258 2770 2258
Size: 512 5255
RsrII CG'GwC_CG
Cuts at: 2903 2903
Size: 5767
SacII CC_GC'GG
Cuts at: 2139 2241 2139
Size: 102 5665
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SapI GCTCTTCn'nnn_
Cuts at: 2594 2594
Size: 5767
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2771 3115 4250 4931 5122 826
Size: 219 1726 344 1135 681 191 1471
Fragments arranged by size:
1726 1471 1135 681 344 219 191
SfoI GGC'GCC
Cuts at: 2092 2124 2320 2419 2652 2875 3040 2092
Size: 32 196 99 233 223 165 4819
Fragments arranged by size:
4819 233 223 196 165 99 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 2979 3094 1872
Size: 1107 115 4545
Fragments arranged by size:
4545 1107 115
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3871 4772 5034 820
Size: 197 926 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 2200 2389 3125 1132
Size: 1068 189 736 3774
Fragments arranged by size:
3774 1068 736 189
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2094 2214 2322 2748 3206 513
Size: 552 1029 120 108 426 458 3074
Fragments arranged by size:
3074 1029 552 458 426 120 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3794 3811 3964 4149 4594 325
Size: 1255 2214 17 153 185 445 1498
Fragments arranged by size:
2214 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3018 4013 5725
Size: 274 80 33 51 1443 1041 995 1712
Cuts at: 5725 96
Size: 138
Fragments arranged by size:
1712 1443 1041 995 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5727 5759 5727
Size: 32 5735
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 2977 3092 1870
Size: 1107 115 4545
Fragments arranged by size:
4545 1107 115
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspXI SacI SanDI SexAI SfiI SrfI SwaI XcmI