(Linear) (Six-base) MAPSORT of: KIEE0789.seq Check: 9977 from: 1 to: 1062 KIEE0789.orf With 182 enzymes: SgfI * December 5, 2014 12:55 .. AccI GT'mk_AC Cuts at: 0 602 1062 Size: 602 460 AgeI A'CCGG_T Cuts at: 0 719 1062 Size: 719 343 AlwNI CAG_nnn'CTG Cuts at: 0 253 329 765 928 1062 Size: 253 76 436 163 134 Fragments arranged by size: 436 253 163 134 76 ApaI G_GGCC'C Cuts at: 0 164 532 790 1062 Size: 164 368 258 272 Fragments arranged by size: 368 272 258 164 AscI GG'CGCG_CC Cuts at: 0 98 1062 Size: 98 964 AvaI C'yCGr_G Cuts at: 0 649 972 1062 Size: 649 323 90 Fragments arranged by size: 649 323 90 BanI G'GyrC_C Cuts at: 0 85 117 313 412 645 828 868 1033 Size: 85 32 196 99 233 183 40 165 Cuts at: 1033 1062 Size: 29 Fragments arranged by size: 233 196 183 165 99 85 40 32 29 BanII G_rGCy'C Cuts at: 0 13 55 164 532 790 1062 Size: 13 42 109 368 258 272 Fragments arranged by size: 368 272 258 109 42 13 BbeI G_GCGC'C Cuts at: 0 89 121 317 416 649 872 1037 1062 Size: 89 32 196 99 233 223 165 25 Fragments arranged by size: 233 223 196 165 99 89 32 25 BglI GCCn_nnn'nGGC Cuts at: 0 159 238 866 977 1062 Size: 159 79 628 111 85 Fragments arranged by size: 628 159 111 85 79 BglII A'GATC_T Cuts at: 0 701 1062 Size: 701 361 Bme1580I G_kGCm'C Cuts at: 0 164 532 790 1062 Size: 164 368 258 272 Fragments arranged by size: 368 272 258 164 BmgBI CAC'GTC Cuts at: 0 1058 1062 Size: 1058 4 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 429 631 955 1062 Size: 429 202 324 107 Fragments arranged by size: 429 324 202 107 Bpu10I CC'TnA_GC Cuts at: 0 49 929 1062 Size: 49 880 133 Fragments arranged by size: 880 133 49 BsaI GGTCTCn'nnnn_ Cuts at: 0 58 1062 Size: 58 1004 BsaHI Gr'CG_yC Cuts at: 0 25 86 118 293 314 413 512 646 Size: 25 61 32 175 21 99 99 134 Cuts at: 646 685 869 919 1025 1034 1062 Size: 39 184 50 106 9 28 Fragments arranged by size: 184 175 134 106 99 99 61 50 39 32 28 25 21 9 BsaWI w'CCGG_w Cuts at: 0 719 1062 Size: 719 343 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 928 958 1062 Size: 928 30 104 Fragments arranged by size: 928 104 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 76 156 952 1062 Size: 76 80 796 110 Fragments arranged by size: 796 110 80 76 BseYI C'CCAG_C Cuts at: 0 525 949 1062 Size: 525 424 113 Fragments arranged by size: 525 424 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 520 949 1062 Size: 520 429 113 Fragments arranged by size: 520 429 113 BsiEI CG_ry'CG Cuts at: 0 137 182 233 269 1004 1062 Size: 137 45 51 36 735 58 Fragments arranged by size: 735 137 58 51 45 36 BsiHKAI G_wGCw'C Cuts at: 0 584 662 1062 Size: 584 78 400 Fragments arranged by size: 584 400 78 BsmBI CGTCTCn'nnnn_ Cuts at: 0 300 1062 Size: 300 762 Bsp1286I G_dGCh'C Cuts at: 0 13 55 164 532 584 662 790 1062 Size: 13 42 109 368 52 78 128 272 Fragments arranged by size: 368 272 128 109 78 52 42 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 588 684 1049 1062 Size: 588 96 365 13 Fragments arranged by size: 588 365 96 13 BsrBI CCG'CTC Cuts at: 0 559 615 1062 Size: 559 56 447 Fragments arranged by size: 559 447 56 BsrFI r'CCGG_y Cuts at: 0 400 481 499 719 895 909 1062 Size: 400 81 18 220 176 14 153 Fragments arranged by size: 400 220 176 153 81 18 14 BssHII G'CGCG_C Cuts at: 0 98 146 1062 Size: 98 48 916 Fragments arranged by size: 916 98 48 BstAPI GCAn_nnn'nTGC Cuts at: 0 253 329 765 857 1062 Size: 253 76 436 92 205 Fragments arranged by size: 436 253 205 92 76 BstXI CCAn_nnnn'nTGG Cuts at: 0 453 525 1062 Size: 453 72 537 Fragments arranged by size: 537 453 72 BstYI r'GATC_y Cuts at: 0 701 1062 Size: 701 361 BtgI C'CryG_G Cuts at: 0 89 131 182 209 233 317 1062 Size: 89 42 51 27 24 84 745 Fragments arranged by size: 745 89 84 51 42 27 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 489 1062 Size: 489 573 EaeI y'GGCC_r Cuts at: 0 134 230 266 395 479 665 888 1001 Size: 134 96 36 129 84 186 223 113 Cuts at: 1001 1062 Size: 61 Fragments arranged by size: 223 186 134 129 113 96 84 61 36 EagI C'GGCC_G Cuts at: 0 134 230 266 1001 1062 Size: 134 96 36 735 61 Fragments arranged by size: 735 134 96 61 36 EarI CTCTTCn'nnn_ Cuts at: 0 589 1062 Size: 589 473 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 791 1062 Size: 791 271 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 429 631 955 1062 Size: 429 202 324 107 Fragments arranged by size: 429 324 202 107 EcoO109I rG'GnC_Cy Cuts at: 0 161 1062 Size: 161 901 HaeII r_GCGC'y Cuts at: 0 89 121 317 416 576 621 649 872 Size: 89 32 196 99 160 45 28 223 Cuts at: 872 1037 1062 Size: 165 25 Fragments arranged by size: 223 196 165 160 99 89 45 32 28 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 928 960 1062 Size: 928 32 102 Fragments arranged by size: 928 102 32 KasI G'GCGC_C Cuts at: 0 85 117 313 412 645 868 1033 1062 Size: 85 32 196 99 233 223 165 29 Fragments arranged by size: 233 223 196 165 99 85 32 29 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 65 1062 Size: 65 997 MscI TGG'CCA Cuts at: 0 667 1062 Size: 667 395 MspA1I CmG'CkG Cuts at: 0 83 133 235 253 534 765 1019 1062 Size: 83 50 102 18 281 231 254 43 Fragments arranged by size: 281 254 231 102 83 50 43 18 NaeI GCC'GGC Cuts at: 0 483 911 1062 Size: 483 428 151 Fragments arranged by size: 483 428 151 NarI GG'CG_CC Cuts at: 0 86 118 314 413 646 869 1034 1062 Size: 86 32 196 99 233 223 165 28 Fragments arranged by size: 233 223 196 165 99 86 32 28 NcoI C'CATG_G Cuts at: 0 89 209 317 1062 Size: 89 120 108 745 Fragments arranged by size: 745 120 108 89 NgoMIV G'CCGG_C Cuts at: 0 481 909 1062 Size: 481 428 153 Fragments arranged by size: 481 428 153 NotI GC'GGCC_GC Cuts at: 0 134 230 266 1001 1062 Size: 134 96 36 735 61 Fragments arranged by size: 735 134 96 61 36 NspI r_CATG'y Cuts at: 0 195 285 384 1062 Size: 195 90 99 678 Fragments arranged by size: 678 195 99 90 PasI CC'CwG_GG Cuts at: 0 123 1062 Size: 123 939 PflMI CCAn_nnn'nTGG Cuts at: 0 404 1062 Size: 404 658 PspOMI G'GGCC_C Cuts at: 0 160 528 786 1062 Size: 160 368 258 276 Fragments arranged by size: 368 276 258 160 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 706 738 1062 Size: 706 32 324 Fragments arranged by size: 706 324 32 PstI C_TGCA'G Cuts at: 0 770 1062 Size: 770 292 PvuII CAG'CTG Cuts at: 0 253 765 1062 Size: 253 512 297 Fragments arranged by size: 512 297 253 RsrII CG'GwC_CG Cuts at: 0 898 1062 Size: 898 164 SacII CC_GC'GG Cuts at: 0 134 236 1062 Size: 134 102 826 Fragments arranged by size: 826 134 102 SapI GCTCTTCn'nnn_ Cuts at: 0 589 1062 Size: 589 473 SfcI C'TryA_G Cuts at: 0 766 1062 Size: 766 296 SfoI GGC'GCC Cuts at: 0 87 119 315 414 647 870 1035 1062 Size: 87 32 196 99 233 223 165 27 Fragments arranged by size: 233 223 196 165 99 87 32 27 SmaI CCC'GGG Cuts at: 0 974 1062 Size: 974 88 SphI G_CATG'C Cuts at: 0 195 384 1062 Size: 195 189 678 Fragments arranged by size: 678 195 189 StyI C'CwwG_G Cuts at: 0 89 209 317 743 1062 Size: 89 120 108 426 319 Fragments arranged by size: 426 319 120 108 89 TatI w'GTAC_w Cuts at: 0 1013 1062 Size: 1013 49 XmaI C'CCGG_G Cuts at: 0 972 1062 Size: 972 90 Enzymes that do cut: AccI AgeI AlwNI ApaI AscI AvaI BanI BanII BbeI BglI BglII Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI BtgI BtgZI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PflMI PspOMI PsrI PstI PvuII RsrII SacII SapI SfcI SfoI SmaI SphI StyI TatI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI BmtI BplI BpuEI BsaAI BsaBI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NdeI NheI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PvuI SacI SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI