Restriction map for
pFN21AE1300
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KasI MaeI MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 70 GACGTC GACGT^C
Acc65I 628 GGTACC G^GTACC
AccII 1350 CGCG CG^CG
AflIII 20 ACATGT A^C[GA][CT]GT
Alw44I 202 GTGCAC G^TGCAC
AlwNI 133 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 202 GTGCAC G^TGCAC
Asp718I 628 GGTACC G^GTACC
AviII 260 TGCGCA TGC^GCA
BsaBI 258 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 279 GTGCAG GTGCAG(16/14)
BsmBI 543 CGTCTC CGTCTC(1/5)
BspLU11I 20 ACATGT A^CATGT
BspMI 137 ACCTGC ACCTGC(4/8)
BstEII 1344 GGTCACC G^GT[ACGT]ACC
BstPI 1344 GGTCACC G^GT[ACGT]ACC
BstUI 1350 CGCG CG^CG
BstXI 1482 CCAGGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 1344 GGTCACC G^GT[ACGT]ACC
FspI 260 TGCGCA TGC^GCA
KpnI 628 GGTACC GGTAC^C
KspI 1349 CCGCGG CCGC^GG
MamI 258 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1647 CATGACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1350 CGCG CG^CG
NcoI 47 CCATGG C^CATGG
PaeR7I 153 CTCGAG C^TCGAG
PflMI 518 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 190 GACTC GAGTC(4/5)
PpuMI 181 GGGACCT [GA]G^G[AT]CC[CT]
SacI 850 GAGCTC GAGCT^C
SacII 1349 CCGCGG CCGC^GG
SbfI 363 CCTGCAGG CCTGCA^GG
SexAI 641 ACCAGGT A^CC[AT]GGT
Sse8387I 363 CCTGCAGG CCTGCA^GG
TfiI 751 GATTC G^A[AT]TC
Van91I 518 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 472 CCAGACCCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 153 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 135 GTAC GT^AC
629 GTAC
AlwI 1634 GGATC GGATC(4/5)
1697 GGATC
BsaI 797 GAGACC GGTCTC(1/5)
1219 GAGACC
BseRI 823 CTCCTC GAGGAG(10/8)
1026 CTCCTC
BsrDI 635 GCAATG GCAATG(2/0)
1487 GCAATG
BstYI 1634 GGATCT [GA]^GATC[CT]
1697 GGATCT
DpnII 1635 GATC ^GATC
1698 GATC
HaeII 487 AGCGCC [GA]GCGC^[CT]
1580 AGCGCC
HinfI 190 GACTC G^A[ACGT]TC
751 GATTC
MboI 1635 GATC ^GATC
1698 GATC
MflI 1634 GGATCT [GA]^GATC[CT]
1697 GGATCT
NdeII 1635 GATC ^GATC
1698 GATC
NspI 20 ACATGT [GA]CATG^[CT]
1676 GCATGT
PstI 364 CTGCAG CTGCA^G
888 CTGCAG
PvuII 702 CAGCTG CAG^CTG
1525 CAGCTG
RsaI 135 GTAC GT^AC
629 GTAC
Sau3AI 1635 GATC ^GATC
1698 GATC
SfcI 364 CTGCAG C^T[GA][CT]AG
888 CTGCAG
SmlI 153 CTCGAG C^T[CT][GA]AG
853 CTCAAG
XhoII 1634 GGATCT [GA]^GATC[CT]
1697 GGATCT
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcyI AluI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI Ksp632I MaeII MaeIII MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI SmaI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XmaCI XmaI